LOCUS       MR231001                7065 bp    DNA     circular  SYN 
DEFINITION  Hgf (myc-DDK-tagged) - Mouse hepatocyte growth factor (Hgf), transcript variant 1
ACCESSION   NM_001289458
VERSION     NM_001289458.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3222..3313 
                     /label="Myc-DDK"  
     misc_feature    3343..3928 
                     /label="PolyA signal"  
     misc_feature    3400..3419 
                     /label="Restriction Enzyme"  
     misc_feature    4204..4876 
                     /label="ColE1"  
     misc_feature    5306..6100 
                     /label="Kan/Neo"  
     misc_feature    6135..6437 
                     /label="SV40 ori"  
     misc_feature    6523..7000 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3204..3210 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3204 
                     /label="Hgf"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATGTGGGGG ACCAAACTTC TGCCGGTCCT GTTGCTGCAG CATGTCCTCC 
     1081 TGCACCTCCT CCTGCTTCAT GTCGCCATCC CCTATGCAGA AGGACAGAAG AAAAGAAGAA 
     1141 ATACACTTCA TGAATTTAAA AAGTCAGCAA AAACTACTCT TACCAAGGAA GACCCATTAC 
     1201 TGAAGATTAA AACCAAAAAA GTGAACTCTG CAGATGAGTG TGCCAACAGG TGTATCAGGA 
     1261 ACAGGGGCTT TACGTTCACT TGCAAGGCCT TCGTTTTTGA TAAGTCAAGA AAACGATGCT 
     1321 ACTGGTATCC TTTCAATAGT ATGTCAAGTG GAGTGAAAAA AGGGTTTGGC CATGAATTTG 
     1381 ACCTCTATGA AAACAAAGAC TATATTAGAA ACTGCATCAT TGGTAAAGGA GGCAGCTATA 
     1441 AAGGGACGGT ATCCATCACT AAGAGTGGCA TCAAATGCCA GCCTTGGAAT TCCATGATCC 
     1501 CCCATGAACA CAGCTTTTTG CCTTCGAGCT ATCGCGGTAA AGACCTACAG GAAAACTACT 
     1561 GTCGAAATCC TCGAGGGGAA GAAGGGGGAC CCTGGTGTTT CACAAGCAAT CCAGAGGTAC 
     1621 GCTACGAAGT CTGTGACATT CCTCAGTGTT CAGAAGTTGA ATGCATGACC TGCAATGGTG 
     1681 AAAGCTACAG AGGTCCCATG GATCACACAG AATCAGGCAA GACTTGTCAG CGCTGGGACC 
     1741 AGCAGACACC ACACCGGCAC AAGTTCTTGC CAGAAAGATA TCCCGACAAG GGCTTTGATG 
     1801 ATAATTATTG CCGCAATCCT GATGGCAAGC CGAGGCCATG GTGCTACACT CTTGACCCTG 
     1861 ACACCCCTTG GGAGTATTGT GCAATTAAAA CGTGCGCTCA CAGTGCTGTG AATGAGACTG 
     1921 ATGTCCCTAT GGAAACAACT GAATGCATTC AAGGCCAAGG AGAAGGTTAC AGGGGAACCA 
     1981 GCAATACCAT TTGGAATGGA ATTCCCTGTC AGCGTTGGGA TTCGCAGTAC CCTCACAAGC 
     2041 ATGATATCAC TCCCGAGAAC TTCAAATGCA AGGACCTTAG AGAAAATTAT TGCCGCAATC 
     2101 CAGATGGGGC TGAATCACCA TGGTGTTTTA CCACTGACCC AAACATCCGA GTTGGCTACT 
     2161 GCTCTCAAAT TCCCAAGTGT GACGTGTCAA GTGGACAAGA TTGTTATCGT GGCAATGGGA 
     2221 AAAATTACAT GGGCAACTTA TCCAAAACAA GGTCTGGACT TACATGTTCC ATGTGGGACA 
     2281 AGAATATGGA GGATTTACAC CGTCATATCT TCTGGGAGCC AGATGCTAGC AAATTGAATA 
     2341 AGAATTACTG CCGGAATCCT GATGATGATG CCCATGGACC TTGGTGCTAC ACGGGGAATC 
     2401 CTCTTATTCC TTGGGATTAT TGCCCTATTT CCCGTTGTGA AGGAGATACT ACACCTACAA 
     2461 TTGTCAATTT GGACCATCCT GTAATATCCT GTGCCAAAAC AAAACAACTG CGGGTTGTAA 
     2521 ATGGCATTCC AACACAAACA ACAGTAGGGT GGATGGTTAG TTTGAAATAC AGAAATAAAC 
     2581 ATATCTGTGG AGGATCATTG ATAAAGGAAA GTTGGGTTCT TACTGCAAGA CAATGTTTTC 
     2641 CAGCCAGAAA CAAAGACTTG AAAGACTATG AAGCTTGGCT TGGCATCCAC GATGTTCATG 
     2701 AGAGAGGCGA GGAGAAGCGC AAGCAGATCT TAAACATTTC CCAGCTGGTC TATGGTCCTG 
     2761 AAGGCTCAGA CTTGGTTTTA CTGAAGCTTG CTCGACCTGC AATCCTGGAT AACTTTGTCA 
     2821 GTACAATTGA TTTACCTAGT TATGGTTGTA CAATCCCTGA AAAGACCACT TGCAGTATTT 
     2881 ACGGCTGGGG CTACACTGGA TTGATCAACG CGGATGGTTT ATTACGAGTA GCTCATCTGT 
     2941 ATATTATGGG GAATGAGAAA TGCAGTCAGC ACCATCAAGG CAAGGTGACT TTGAATGAGT 
     3001 CTGAGTTATG TGCTGGGGCT GAAAAGATTG GATCAGGACC ATGTGAGGGA GATTATGGTG 
     3061 GCCCACTCAT TTGTGAACAA CACAAAATGA GAATGGTTCT TGGTGTCATT GTTCCTGGTC 
     3121 GTGGATGTGC CATCCCAAAT CGTCCTGGTA TTTTTGTTCG AGTAGCATAT TATGCAAAAT 
     3181 GGATACACAA AGTAATTTTG ACATACAAGT TGACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     3241 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     3301 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     3361 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     3421 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     3481 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     3541 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     3601 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     3661 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     3721 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     3781 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     3841 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     3901 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     3961 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     4021 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     4081 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     4141 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     4201 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     4261 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     4321 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     4381 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     4441 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     4501 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     4561 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     4621 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     4681 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     4741 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     4801 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     4861 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     4921 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     4981 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     5041 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     5101 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     5161 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     5221 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     5281 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     5341 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     5401 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     5461 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     5521 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     5581 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     5641 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     5701 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     5761 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     5821 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     5881 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     5941 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     6001 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     6061 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     6121 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     6181 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     6241 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     6301 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     6361 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     6421 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     6481 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     6541 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     6601 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     6661 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     6721 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     6781 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     6841 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     6901 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     6961 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     7021 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//