LOCUS       MR230990                7056 bp    DNA     circular  SYN 
DEFINITION  Add2 (myc-DDK-tagged) - Mouse adducin 2 (beta) (Add2), transcript variant 1
ACCESSION   NM_001271857
VERSION     NM_001271857.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3213..3304 
                     /label="Myc-DDK"  
     misc_feature    3334..3919 
                     /label="PolyA signal"  
     misc_feature    3391..3410 
                     /label="Restriction Enzyme"  
     misc_feature    4195..4867 
                     /label="ColE1"  
     misc_feature    5297..6091 
                     /label="Kan/Neo"  
     misc_feature    6126..6428 
                     /label="SV40 ori"  
     misc_feature    6514..6991 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3195..3201 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3195 
                     /label="Add2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGCGAGGAC ACGGTTCCCG AGGCGGCCTC CCCGCCACCC TCTCAGGGGC 
     1081 AGCACTACTT TGACCGGTTC TCTGAAGATG ATCCTGAGTA CTTGCGACTT CGCAACCGTG 
     1141 CAGCTGACCT GCGACAGGAC TTCAACTTGA TGGAGCAGAA GAAGAGGGTC ACCATGATCC 
     1201 TGCAGAGCCC TTCTTTCAGG GAGGAGCTGG AAGGCCTCAT CCAGGAGCAA ATGAAGAAGG 
     1261 GCAACAACTC CTCCAACATC TGGGCCCTCC GACAGATCGC GGACTTCATG GCCAGCACCT 
     1321 CCCACGCAGT CTTCCCAGCT TCTTCCATGA ACTTCTCCAT GATGACACCC ATCAACGACC 
     1381 TCCACACTGC CGACTCCCTG AACCTGGCCA AGGGGGAGAG GCTTATGCGG TGCAAGATCA 
     1441 GCAGCGTCTA CCGTCTCCTG GACCTCTACG GCTGGGCGCA GCTCAGTGAC ACCTACGTCA 
     1501 CGCTGAGAGT GAGCAAGGAG CAGGACCACT TCCTGATCAG CCCCAAGGGG GTTTCCTGCA 
     1561 GCGAGGTCAC AGCCTCCAGC CTGATTAAAG TGAACATTCT GGGAGAGGTG GTGGAGAAGG 
     1621 GCAGCAGTTG CTTCCCGGTG GACACCACGG GCTTCAGTCT GCACTCAGCC ATCTATGCCG 
     1681 CCAGGCCCGA CGTGCGGTGT GCCATCCACC TGCACACGCC TGCCACCGCA GCGGTGTCAG 
     1741 CTATGAAGTG CGGCCTCCTG CCGGTCTCCC ATAATGCCCT GCTGGTGGGG GACATGGCCT 
     1801 ACTATGACTT CAATGGGGAA ATGGAGCAGG AAGCTGACCG AATCAACTTG CAGAAGTGCC 
     1861 TTGGACCCAC CTGCAAGATT CTGGTGCTAA GAAACCATGG CATGGTCGCC CTGGGTGACA 
     1921 CCGTGGAGGA AGCTTTCTAC AAGGTCTTCC ATCTGCAGGC TGCGTGTGAG GTACAGGTGT 
     1981 CGGCTCTGTC CAGTGCTGGG GGCACTGAGA ACCTCATCCT CTTGGAGCAA GAGAAACACC 
     2041 GGCCGCACGA GGTGGGCTCT GTGCAGTGGG CCGGCAGCAC CTTCGGGCCC ATGCAGAAGA 
     2101 GCCGGCTGGG AGAGCATGAA TTTGAAGCCC TCATGAGGAT GCTGGACAAT TTAGGCTACA 
     2161 GAACAGGCTA TACGTACCGC CACCCCTTTG TCCAAGAGAA AACCAAACAC AAAAGTGAAG 
     2221 TGGAGATCCC AGCCACAGTC ACTGCCTTTG TGTTTGAAGA GGATGGAGTC CCAGTCCCCG 
     2281 CCCTGCGCCA GCACGCCCAG AAGCAGCAGA AGGAAAAGAC CCGCTGGCTT AACACTCCCA 
     2341 ACACCTACCT GCGGGTGAAC GTGGCTGACG AGGTGCAGAG GAACATGGGC AGTCCCCGAC 
     2401 CAAAGACCAC GTGGATGAAG GCTGATGAAG TGGAAAAGTC CAGCAGCGGC ATGCCCATAC 
     2461 GGATTGAAAA CCCAAACCAA TTTGTGCCTC TCTACACTGA CCCCCAGGAA GTTCTGGACA 
     2521 TGAGGAACAA GATTCGAGAG CAAAACCGAC AAGACATAAA GTCAGCCGGG CCTCAGTCTC 
     2581 AGCTCTTGGC CAGTGTCATC GCAGAGAAGA GCCGGAGTCC GTCTACAGAG AGCCAGCTGA 
     2641 TGTCCAAGGG CGATGCAGAT ACCAAAGATG AATCGGAGGA GACGGTGCCC AACCCCTTCA 
     2701 GCCAACTCAC TGACCAGGAG CTGGAGGAGT ACAAGAAGGA GGTGGAAAGG AAGAAGCTAG 
     2761 AACAGGAGCA GGAAGGGGAG AAGGACATAG CCACAGAGAA GCCTGGTTCA CCTGTAAAGT 
     2821 CCACACCTGC ATCCCCAGTG CAGAGCCCAT CAAAAGCCGG GACCAAGAGC CCAGCGGTCT 
     2881 CTCCTTCCAA GACTTCAGAG GATACCAAGA AGACAGAAGT CAGCGAAGCC AACACAGAGC 
     2941 CTGAGCCAGT GAAACCAGAA GGGCTGGTGG TGAATGGGAA GGAGGAGGAG CCGAGCGTAG 
     3001 AAGAGGCCCT CAGCAAAGGA CTGGGCCAGA TGACCACCAA CGCTGACACT GATGGCGACA 
     3061 GCTACAAGGA CAAAACCGAG TCAGTTACCA GTGGCCCCTT GTCCCCAGAG GGCTCGCCCT 
     3121 CTAAGTCACC CTCCAAAAAG AAAAAGAAAT TCCGAACCCC CTCGTTCCTG AAGAAAAGCA 
     3181 AGAAGAAGGA GAAGGTGGAA TCCACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     3241 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     3301 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     3361 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     3421 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     3481 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     3541 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     3601 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     3661 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     3721 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     3781 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     3841 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     3901 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     3961 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     4021 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     4081 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     4141 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     4201 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     4261 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     4321 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     4381 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     4441 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     4501 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     4561 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     4621 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     4681 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     4741 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     4801 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     4861 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     4921 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     4981 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     5041 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     5101 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     5161 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     5221 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     5281 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     5341 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     5401 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     5461 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     5521 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     5581 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     5641 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     5701 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     5761 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     5821 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     5881 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     5941 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     6001 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     6061 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     6121 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     6181 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     6241 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     6301 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     6361 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     6421 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     6481 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     6541 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     6601 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     6661 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     6721 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     6781 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     6841 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     6901 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     6961 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     7021 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//