LOCUS       MR230884                6939 bp    DNA     circular  SYN 
DEFINITION  Cast (myc-DDK-tagged) - Mouse calpastatin (Cast), transcript variant 4
ACCESSION   NM_001301156
VERSION     NM_001301156.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3096..3187 
                     /label="Myc-DDK"  
     misc_feature    3217..3802 
                     /label="PolyA signal"  
     misc_feature    3274..3293 
                     /label="Restriction Enzyme"  
     misc_feature    4078..4750 
                     /label="ColE1"  
     misc_feature    5180..5974 
                     /label="Kan/Neo"  
     misc_feature    6009..6311 
                     /label="SV40 ori"  
     misc_feature    6397..6874 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3078..3084 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3078 
                     /label="Cast"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGTACCACA GAGACTAAGG CTGTGAAAAC AGAGTCTAAG AAACCACAAT 
     1081 CAAGTGAGCA ACCTGTGGTT CACGAGAAAA AGTCAAAAGG AGGGCCAAAG GAAGGGTCAG 
     1141 AGCCCAAAAA TCTTCCGAAG CACACATCAA GTACAGGAAG CAAGCATGCT CATAAAGAAA 
     1201 AAGCACTTTC CAGATCAAAT GAACAGATGG TGTCAGAGAA ACCATCAGAA TCAAAGACAA 
     1261 AATTTCAAGA TGTTCCTTCA GCTGGTGGAG AGAGTGTGGC TGGAGGTGGG ACTGTGGCCA 
     1321 CTGCCTTGGA CAAAGTGGTT GGTAAGAAAA AAGAACAGAA ACCATTCACA CCAGCTTCAC 
     1381 CTGTGCAGTC CACACCGAGC AAGCCATCTG ACAAGTCAGG CATGGATGCT GCCTTGGATG 
     1441 ACCTGATAGA CACCTTAGGT GGACATGAAG ATACTAACAG AGATGATCCA CCATATACTG 
     1501 GACCAGTAGT TTTGGACCCA ATGTATTCTA CCTACCTTGA GGCACTGGGT ATAAAAGAAG 
     1561 GGACTATTCC TCCAGAGTAT AGGAAACTTT TGGAAAAAAA TGAAGGGATC ACACAACCTC 
     1621 TTCCAGACTC TCCTAAACCT ATGGGGACTG ACCAGGCTAT AGATGCCTTG TCATCTGATT 
     1681 TCACCTGTAG TTCTCCAACT GGAAAGCAAA GTGAGAAAGA GAAGTCTACT GGGGAAATTT 
     1741 TCAAAGCTCA GTCTGCAGGA GTGACCAGAA GTTCTGTTCC CCCCAAGGAG AAGAAAAGGA 
     1801 AGGTGGAAGA GGAGGTCATA AATGATCAAG CACTTCAGGC TCTGTCAGAT TCACTGGGCA 
     1861 CCCGGCAGCC AGATCCTCCG AGCCACGTTA GCCAAGCTGA ACAAGTCAAA GAGGCAAAAG 
     1921 CAAAAGAAGA AAGGCAGGAG AAGTGTGGTG AAGATGAGGA CACAGTCCCA GCTGAGTACA 
     1981 GGTTAAAACC AGCAAAGGAT AAAGATGGAA AACCACTATT GCCAGAGCCT GAAGAAACAT 
     2041 CTAAGAGCCT GAGTGAGTCG GAGCTGATTG GGGAGCTTTC AGCAGATTTT GACCGATCTA 
     2101 CATATCAAGA CAAACCATCT ACGCCAGCTG AAAAGAAATC CAATGACACA TCCCAAACTC 
     2161 CTCCGGGGGA GACTGTGCCT CGGGCCTCCA TGTGCAGTAT ACGGTCAGCG CCACCCAAAC 
     2221 TAGCATCCTT GAAGGGCGTG GTACCAGAAG ATGCTGTTGA AACCTTGGCT GGAAGCCTGG 
     2281 GGACAAGGGA AGCAGATCCA GAACATGAAA AAACTGTGGA GGATAAAGTC AAGGAGAAAG 
     2341 CTAAAGAAGA AGAGCATGAA AAACTTGGTG AAAAAGAAGA AACAGTGCCT CCTGATTATC 
     2401 GACTAGAAGA AGTCAAGGAT AAGGATGGAA AACCACTCCT GCCCAAAGAA TCCCAGGAAC 
     2461 AACTTGCACC CTTAAGCGAT GACTTCCTTC TTGATGCCTT GTCTCAGGAC TTCTCCAGTC 
     2521 CGGCAAACAT ATCGTCTCTT GAATTTGAAG ATGCCAAACT TTCTGCTGCC ATTTCTGAAG 
     2581 TAGTTTCTCA GACACCTGCT CCAAGCACCC ATGCAGCAGC TCCACTGCCT GGCACTGAGC 
     2641 AGAAAGACAA AGAACTTGAT GATGCCTTGG ATGAACTTTC TGACAGTCTT GGACAAAGGC 
     2701 CGCCTGATCC AGATGAGAAC AAACCACTGG ATGACAAAGT GAAGGAGAAA ATCAAACCAG 
     2761 AGCATAGCGA GAAACTGGGA GAAAGAGACG ACACCATCCC CCCTGAATAC AGGCATCTCT 
     2821 TGGATAATGA TGGGAAGGAC AAACCAGAGA AGCCACCGAC TAAGAAAACA GAGAAACCTG 
     2881 ATCAGGACCG GGACCCCATT GATGCCCTCT CAGAAGATTT GGATAGCTGC CCCTCAACTA 
     2941 CAGAGACCTC AAAGAATACA GCAAAGGGGA AGAGCAAGAA GACTTCAAGT TCCAAAGCAT 
     3001 CCAAGGACGG AGAGAAAACA AAGGACTCTT CCAAGAAGAC AGAGGAAGTG TCCAAGCCAA 
     3061 AGGCTAAAGA AGATGCAAGA CACAGTACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     3121 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     3181 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     3241 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     3301 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     3361 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     3421 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     3481 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     3541 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     3601 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     3661 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     3721 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     3781 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     3841 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     3901 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     3961 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     4021 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     4081 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     4141 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     4201 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     4261 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     4321 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     4381 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     4441 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     4501 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     4561 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     4621 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     4681 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     4741 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     4801 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     4861 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     4921 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     4981 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     5041 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     5101 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     5161 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     5221 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     5281 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     5341 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     5401 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     5461 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     5521 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     5581 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     5641 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     5701 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     5761 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     5821 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     5881 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     5941 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     6001 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     6061 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     6121 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     6181 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     6241 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     6301 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     6361 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     6421 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     6481 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     6541 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     6601 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     6661 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     6721 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     6781 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     6841 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     6901 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//