LOCUS       MR230828                6882 bp    DNA     circular  SYN 
DEFINITION  Mid1 (myc-DDK-tagged) - Mouse midline 1 (Mid1), transcript variant 3
ACCESSION   NM_001290504
VERSION     NM_001290504.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3039..3130 
                     /label="Myc-DDK"  
     misc_feature    3160..3745 
                     /label="PolyA signal"  
     misc_feature    3217..3236 
                     /label="Restriction Enzyme"  
     misc_feature    4021..4693 
                     /label="ColE1"  
     misc_feature    5123..5917 
                     /label="Kan/Neo"  
     misc_feature    5952..6254 
                     /label="SV40 ori"  
     misc_feature    6340..6817 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3021..3027 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3021 
                     /label="Mid1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAAACACTG GAGTCGGAGC TGACCTGTCC TATTTGTCTG GAGCTTTTTG 
     1081 AGGACCCTCT CCTGCTTCCC TGCGCACATA GCCTCTGCTT CAACTGTGCC CACCGAATCC 
     1141 TGGTCTCTCA CTGTGCCACC AACGAGCCTG TGGAGTCCAT CAACGCCTTC CAGTGCCCCA 
     1201 CCTGCCGGCA TGTCATCACG CTCAGCCAGC GAGGTCTAGA CGGGCTCAAG CGCAACGTCA 
     1261 CCCTCCAAAA CATCATTGAC AGATTTCAGA AAGCATCTGT GAGCGGGCCC AACTCTCCCA 
     1321 GTGAGACCCG CAGGGAACGG GCCTTTGACG CCAACACCAT GTCCTCTGCC GAGAAGGTTC 
     1381 TCTGCCAGTT CTGTGACCAG GATCCTGCCC AGGATGCTGT GAAGACTTGC GTTACTTGTG 
     1441 AAGTGTCCTA CTGTGATGAG TGCCTGAAAG CCACTCATCC GAACAAGAAG CCCTTTACAG 
     1501 GCCATCGTCT GATTGAGCCA ATCCCGGACT CGCACATCCG GGGGCTGATG TGCCTGGAGC 
     1561 ACGAGGATGA GAAGGTGAAT ATGTACTGTG TGACCGATGA CCAGTTAATC TGTGCCTTGT 
     1621 GTAAACTGGT TGGGCGGCAC CGCGATCATC AGGTGGCAGC TTTGAGTGAG CGCTATGACA 
     1681 AATTGAAGCA AAACTTGGAG AGTAATCTCA CCAATCTTAT TAAGAGAAAC ACAGAACTGG 
     1741 AGACTCTTTT GGCTAAACTC ATCCAAACTT GTCAACATGT TGAAGTCAAT GCATCCCGTC 
     1801 AAGAAGCCAA ACTGACAGAA GAATGTGATC TTCTCATTGA AATCATTCAG CAACGAAGAC 
     1861 AAATTATTGG AACAAAGATT AAAGAAGGCA AGGTGATCAG GCTCCGCAAG TTAGCTCAGC 
     1921 AGATTGCAAA CTGTAAACAG TGCCTTGAGA GGTCTGCATC GCTCATCTCG CAAGCGGAGC 
     1981 ACTCGCTGAA GGAAAATGAC CACGCCCGTT TTCTACAGAC AGCAAAGAAT ATCACTGAGA 
     2041 GAGTCTCCAT GGCAACTGCA TCCTCCCAGG TCCTAATTCC CGAAATCAAC CTCAATGACA 
     2101 CGTTTGACAC TTTTGCCTTG GATTTTTCCC GGGAGAAGAA ACTGCTAGAA TGTCTGGATT 
     2161 ACCTAACAGC TCCCAACCCT CCCGCGATTA GAGAAGAGCT CTGCACCGCT TCCTACGACA 
     2221 CCATCACCGT CCACTGGACC TCAGAGGACG AGTTCAGCGT GGTCTCCTAC GAGCTCCAGT 
     2281 ACACCATATT CACCGGACAA GCCAATGTTG TCAGTCTGTG TAACTCGGCG GACAGCTGGA 
     2341 TGATCGTGCC CAACATCAAG CAGAACCACT ACACCGTGCA CGGCCTGCAA AGTGGCACCA 
     2401 AGTATATCTT CACGGTGAAG GCCATCAACC AGGCGGGCAG CCGTAGCAGC GAGCCCGGAA 
     2461 AGCTGAAGAC CAACAGTCAG CCGTTTAGAC TGGATCCCAA ATCGGCTCAT CGCAAGCTGA 
     2521 AGGTGTCCCA CGACAACCTG ACTGTCGAGC GCGACGAGTC GTCCTCTAAG AAGAGTCACG 
     2581 CGCCGGAGCG CTTCGCTGGT CAGGGGAGCT ACGGAGTGGC TGGCAACGTG TTCATCGACA 
     2641 GCGGCCGTCA CTACTGGGAA GTGGTCACCA GCGGAAGCAC ATGGTACGCC ATCGGCCTGG 
     2701 CGTACAGATC GGCGCCGAAA CACGAGTGGA TCGGGAAGAA CGCGGCGTCC TGGGCCCTCT 
     2761 GCCGCTGCCA CAACCACTGG GCGGTGCGAC ACGACGGCAA GGAGACCCCC ATCGCGCCGG 
     2821 CCCCTCACCT CAGGCGCGTC GGCGTCCTGC TGGACTACGA CAACGGCTCC ATCGCCTTCT 
     2881 ACGACGCTCT GAGCTCCGTC CACCTCCACA CCTTCCACGC GGCGCTCGCG CAGCCCGTGT 
     2941 GCCCCACCTT CACCGTGTGG AACAAGTGTC TGACCATCGT CACGGGTCTG CCCATCCCGG 
     3001 ACCATCTGGA CTGTACGGAG CAGCGACCTA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     3061 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     3121 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     3181 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     3241 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     3301 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     3361 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     3421 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     3481 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     3541 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     3601 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     3661 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     3721 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     3781 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     3841 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     3901 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     3961 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     4021 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     4081 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     4141 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     4201 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     4261 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     4321 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     4381 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     4441 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     4501 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     4561 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     4621 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     4681 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     4741 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     4801 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     4861 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     4921 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     4981 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     5041 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     5101 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     5161 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     5221 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     5281 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     5341 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     5401 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     5461 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     5521 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     5581 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     5641 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     5701 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     5761 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     5821 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     5881 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     5941 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     6001 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     6061 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     6121 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     6181 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     6241 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     6301 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     6361 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     6421 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     6481 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     6541 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     6601 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     6661 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     6721 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     6781 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     6841 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//