LOCUS       MR230774                6810 bp    DNA     circular  SYN 
DEFINITION  Rtn3 (myc-DDK-tagged) - Mouse reticulon 3 (Rtn3), transcript variant 5
ACCESSION   NM_001271486
VERSION     NM_001271486.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2967..3058 
                     /label="Myc-DDK"  
     misc_feature    3088..3673 
                     /label="PolyA signal"  
     misc_feature    3145..3164 
                     /label="Restriction Enzyme"  
     misc_feature    3949..4621 
                     /label="ColE1"  
     misc_feature    5051..5845 
                     /label="Kan/Neo"  
     misc_feature    5880..6182 
                     /label="SV40 ori"  
     misc_feature    6268..6745 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2949..2955 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2949 
                     /label="Rtn3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGAGTCG TCAGCGGCCA CTCAGTCCCC GTCAGTCTCC TCGTCGTCCT 
     1081 CCGGGGCCGA GCCGTCAGCT CTCGGCGGCG GCGGCGGGAG CCCTGGAGCC TGCCCCGCCC 
     1141 TGGGGGCGAA GAGCTGCGGC TCCTCGTGTG CGGATTCCTT TGTTTCTTCC TCTTCCTCTC 
     1201 AGCCTGTATC TATATTTTCG ACCTCACAAG GTGACTGGAC AGAAGCATTT ACAGAAGGAA 
     1261 AACCTGTAAG AGACTACCTC AGTTCCACAA AAGAAGCTGG TGGCAACGGT GTGCCAGGCA 
     1321 GTTCTCAGCT TCATTCTGAG CTGCCTGGCT CTATGCCTGA GAAATGGGTC TCAGGCTCTG 
     1381 GAGCAGCCAC AGTGGAAGTA ACTTTACCTA ACCTGAGGGG TGCCTGGCCT AACTCTGTGA 
     1441 TGGGGGAAGT CACAGAGGTT GATAGTTCTG GGGAATCTGA TGACACAGTA ATAGAGGACA 
     1501 TCACAGAAAA ACCTGACTCC CTTCCAAGTG CTGCTGCAAA AACAAGTGAA AGGGAAATCA 
     1561 AAGAGACTCC CAGTCGTGAA ACTGTGAGGA GTGAAATGTG TGAAAACTCT GAGCAGCCGC 
     1621 AGGCCCAGCC AGAAACTCCT ACTCAGAAGA GTCTGGAAGG TGAGGTGGCT TCACAAGTAC 
     1681 CTAATACCCT GAATGAAGTC ACACCTGAAA AGCTTGATAT GACTAACAAC CCCAAAGTTT 
     1741 GCTCAGCAGC ACCTCCAAGT GTTCTTAATG AGACAGGATT CTCACTAACT GTGCCAGCTT 
     1801 CTGCCAAGTT GGAATCTTTG CTTGGAAAAT ATGTTGAAGA TACAGATGGT TCCTCCCCAG 
     1861 AGGACTTGAT GGCTGTCCTC ACAGGAGCCG AGGAGAAGGG GATAGTGGAT AAAGAGGAAG 
     1921 GTGATGTTTT GGAAGCAGTG TTAGAGAAGA TAGCAGACTT TAAAAACACT TTGCCTGTGG 
     1981 AACTCTTGCA TGAAAGTGAG CTAAGTGGTT CTGAAACCAA AAATATTAAA AGCAAATACA 
     2041 GTGAAGACAG CAGAGAAACA ACTGGAGGTG CCCCTACGAT GTCTCCCGAC TTAGAGCAGG 
     2101 AGCAGCTCAC CATCAGAGCC ATTAAAGAAT TAGGAGAAAG GCAAGCTGAG AAGGTGCAGG 
     2161 ATGAAGGAAT ATCTTCTGGA GGAAAACTCA AGCAAACCTT TGCTCCACAG TCTGGGCCAC 
     2221 AGAGTTCATC TGACATCCTA GAACACACAG ATGTCAAAAC TGGATCTGAT CTTGGAATTC 
     2281 CCAAAAATCC TACTATCATC AAAAACACTA GAATAGATTC TATTTCCAGC CTTACCAAGA 
     2341 CTGAAATGGT TAACAAGAAT GTTCTAGCAA GACTTCTCAG TGATTTCCCA GTGCACGATC 
     2401 TGATTTTCTG GCGAGATGTG AAGAAGACTG GGTTTGTCTT TGGCACCACA CTGATCATGC 
     2461 TGCTCTCTCT GGCAGCCTTC AGTGTTATCA GTGTGGTCTC TTACCTCATC CTGGCTCTTC 
     2521 TCTCTGTCAC CATCAGCTTC AGAGTCTATA AGTCTGTCAT TCAAGCTGTG CAGAAGTCAG 
     2581 AAGAAGGACA TCCATTCAAG GCCTACTTGG ATGTGGACAT TACCCTGTCT TCAGAAGCTT 
     2641 TCCACAACTA CATGAATGCT GCGATGGTGC ATGTCAACAA GGCCCTCAAA CTCATTATTC 
     2701 GTCTCTTTCT GGTAGAAGAC TTGGTTGACT CCTTGAAGCT GGCTGTCTTC ATGTGGCTGA 
     2761 TGACCTATGT TGGTGCTGTT TTTAATGGAA TTACCCTTCT GATTCTCGCC GAGCTGCTTG 
     2821 TCTTCAGCGT CCCAATTGTC TATGAGAAGT ATAAGACACA GATTGACCAC TATGTTGGGA 
     2881 TTGCCCGGGA TCAGACCAAG TCAATTGTTG AAAAGATCCA AGCAAAGCTT CCTGGAATCG 
     2941 CCAAAAAAAA GGCAGAAACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     3001 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     3061 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     3121 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     3181 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     3241 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     3301 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     3361 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     3421 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     3481 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     3541 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     3601 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     3661 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     3721 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     3781 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     3841 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     3901 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     3961 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     4021 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     4081 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     4141 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     4201 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     4261 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     4321 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     4381 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     4441 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     4501 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     4561 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     4621 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     4681 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     4741 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     4801 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     4861 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     4921 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     4981 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     5041 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     5101 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     5161 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     5221 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     5281 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     5341 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     5401 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     5461 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     5521 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     5581 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     5641 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     5701 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     5761 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     5821 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     5881 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     5941 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     6001 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     6061 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     6121 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     6181 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     6241 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     6301 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     6361 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     6421 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     6481 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     6541 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     6601 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     6661 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     6721 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     6781 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//