LOCUS       MR230533                6573 bp    DNA     circular  SYN 
DEFINITION  Zyx (myc-DDK-tagged) - Mouse zyxin (Zyx), transcript variant 2
ACCESSION   NM_001289617
VERSION     NM_001289617.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2730..2821 
                     /label="Myc-DDK"  
     misc_feature    2851..3436 
                     /label="PolyA signal"  
     misc_feature    2908..2927 
                     /label="Restriction Enzyme"  
     misc_feature    3712..4384 
                     /label="ColE1"  
     misc_feature    4814..5608 
                     /label="Kan/Neo"  
     misc_feature    5643..5945 
                     /label="SV40 ori"  
     misc_feature    6031..6508 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2712..2718 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2712 
                     /label="Zyx"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGCCCCC CGCCCGCCTC CCGCGATCTC CGTCTCCGTC TCGGCCCCCG 
     1081 CGTTTTACGC CCCGCAGAAG AAGTTCGCCC CGGTTGTGGC CCCAAAGCCC AAAGTGAATC 
     1141 CTTTCCGGCC TGGGGACAGC GAGCCTCCTG TAGCAGCCGG GGCCCAAAGA GCGCAGATGG 
     1201 GTCGGGTGGG CGAGATCCCA CCACCACCCC CGGAAGACTT TCCTTTGCCC CCTCCTCCCC 
     1261 TTATTGGGGA GGGCGACGAC TCAGAGGGTG CCCTGGGAGG TGCCTTCCCA CCTCCACCTC 
     1321 CCCCGATGAT CGAGGAACCA TTCCCCCCTG CTCCTCTGGA GGAGGACATC TTCCCCTCCC 
     1381 CTCCACCTCC ACTGGAGGAG GAGGGAGGGC CTGAGGCCCC TACCCAGCTC CCACCGCAGC 
     1441 CCAGGGAGAA AGTGTGCAGT ATTGACCTGG AGATTGACTC TCTGTCCTCA CTGCTGGACG 
     1501 ACATGACCAA GAACGATCCC TTCAAAGCCC GGGTATCATC CGGATATGTA CCCCCACCAG 
     1561 TTGCCACTCC ATTTGTTCCC AAGCCTAGTA CCAAACCTGC CCCTGGGGGC ACAGCACCCT 
     1621 TGCCTCCTTG GAAGACCCCT TCTAGCTCCC AGCCACCACC TCAGCCGCAG GCCAAGCCTC 
     1681 AGGTCCAGCT CCATGTCCAG CCTCAGGCCA AGCCCCATGT CCAACCCCAG CCTGTGTCTT 
     1741 CTGCTAATAC ACAGCCCCGG GGTCCCCTTT CTCAGGCACC AACTCCAGCA CCTAAGTTTG 
     1801 CTCCAGTGGC TCCTAAATTT ACTCCCGTGG TTTCCAAGTT CAGCCCTGGT GCTCCAAGTG 
     1861 GACCTGGGCC ACAGCCCAAT CAAAAAATGG TGCCTCCGGA TGCTCCTTCT TCTGTGAGCA 
     1921 CAGGCTCCCC TCAGCCCCCT AGCTTCACCT ATGCTCAGCA GAAGGAGAAG CCCCTAGTTC 
     1981 AAGAGAAGCA GCACCCACAG CCTCCACCAG CTCAAAACCA AAACCAGGTA CGCTCTCCTG 
     2041 GAGGCCCAGG CCCCTTGACC CTGAAGGAGG TAGAGGAGTT GGAGCAGCTG ACCCAGCAGC 
     2101 TGATGCAGGA CATGGAACAC CCTCAGAGGC AGAGCGTGGC AGTGAATGAG TCCTGTGGCA 
     2161 AATGCAATCA GCCACTGGCC CGTGCACAGC CTGCGGTTCG TGCACTGGGA CAACTGTTCC 
     2221 ACATCACCTG CTTCACTTGC CATCAGTGTC AGCAGCAGCT GCAGGGACAG CAGTTCTATA 
     2281 GCCTGGAGGG AGCACCATAT TGTGAGGGCT GCTACACCGA CACTTTGGAG AAGTGCAACA 
     2341 CCTGTGGGCA GCCCATCACT GACCGCATGC TGAGGGCCAC TGGCAAAGCC TACCACCCAC 
     2401 AGTGCTTCAC CTGTGTGGTC TGCGCCTGTC CCCTGGAGGG CACCTCCTTC ATTGTGGACC 
     2461 AGGCCAATCA GCCCCACTGT GTCCCTGACT ATCACAAGCA ATACGCTCCA AGGTGCTCCG 
     2521 TCTGCTCGGA GCCAATCATG CCTGAGCCTG GCCGAGACGA GACTGTGCGA GTAGTGGCGC 
     2581 TGGATAAGAA CTTTCATATG AAGTGTTACA AGTGTGAGGA CTGTGGGAAA CCTCTGTCCA 
     2641 TTGAGGCAGA TGACAACGGC TGTTTCCCTC TGGATGGCCA CGTCCTTTGT CGGAAGTGCC 
     2701 ACTCCGCTAG AGCCCAGACC ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     2761 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     2821 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     2881 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     2941 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     3001 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     3061 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     3121 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     3181 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     3241 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     3301 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     3361 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     3421 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     3481 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     3541 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     3601 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     3661 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     3721 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     3781 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     3841 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     3901 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     3961 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     4021 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     4081 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     4141 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     4201 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     4261 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     4321 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     4381 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     4441 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     4501 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     4561 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     4621 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     4681 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     4741 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     4801 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     4861 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     4921 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     4981 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     5041 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     5101 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     5161 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     5221 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     5281 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     5341 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     5401 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     5461 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     5521 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     5581 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     5641 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     5701 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     5761 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     5821 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     5881 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     5941 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     6001 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     6061 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     6121 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     6181 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     6241 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     6301 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     6361 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     6421 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     6481 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     6541 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//