LOCUS       MR227598                9138 bp    DNA     circular  SYN 
DEFINITION  Col2a1 (Myc-DDK-tagged) - Mouse collagen, type II, alpha 1 (Col2a1), transcript variant 2
ACCESSION   NM_001113515
VERSION     NM_001113515.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5295..5386 
                     /label="Myc-DDK"  
     misc_feature    5416..6001 
                     /label="PolyA signal"  
     misc_feature    5473..5492 
                     /label="Restriction Enzyme"  
     misc_feature    6277..6949 
                     /label="ColE1"  
     misc_feature    7379..8173 
                     /label="Kan/Neo"  
     misc_feature    8208..8510 
                     /label="SV40 ori"  
     misc_feature    8596..9073 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5277..5283 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5277 
                     /label="Col2a1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATCCGCCTC GGGGCTCCCC AGTCGCTGGT GCTGCTGACG CTGCTCATCG 
     1081 CCGCGGTCCT ACGGTGTCAG GGCCAGGATG CCCGAAAATT AGGGCCAAAG GGGCAGAAAG 
     1141 GAGAACCTGG AGATATCAGA GATATCATAG GACCCAGAGG ACCTCCTGGC CCTCAGGGAC 
     1201 CTGCAGGTGA ACAAGGACCC AGAGGTGATC GTGGTGACAA GGGAGAAAAG GGTGCGCCTG 
     1261 GACCCCGTGG CAGAGATGGA GAACCTGGTA CCCCTGGAAA TCCTGGCCCC GCTGGCCCTC 
     1321 CAGGTCCCCC TGGTCCCCCT GGCCTTAGTG CAGGAAACTT CGCGGCTCAG ATGGCTGGAG 
     1381 GGTATGACGA GAAGGCTGGT GGTGCCCAGA TGGGAGTCAT GCAAGGGCCC ATGGGCCCCA 
     1441 TGGGACCCCG TGGACCCCCA GGCCCTGCCG GTGCCCCCGG CCCTCAAGGA TTTCAAGGCA 
     1501 ATCCTGGTGA ACCTGGCGAG CCTGGTGTCT CTGGTCCCAT GGGTCCCCGA GGTCCTCCTG 
     1561 GCCCTGCTGG AAAACCTGGT GACGACGGTG AAGCTGGGAA GCCCGGAAAG TCTGGGGAAA 
     1621 GAGGCCTCCC TGGCCCTCAG GGTGCTCGTG GATTCCCAGG AACCCCGGGT CTCCCCGGTG 
     1681 TCAAGGGTCA CAGAGGTTAC CCAGGCCTCG ACGGTGCTAA GGGGGAAGCT GGTGCTCCGG 
     1741 GTGTGAAGGG TGAGAGTGGT TCCCCTGGTG AGAACGGATC CCCGGGCCCA ATGGGTCCCC 
     1801 GTGGCCTGCC TGGTGAGAGA GGACGGACTG GCCCTGCTGG TGCTGCTGGT GCTCGGGGTA 
     1861 ACGATGGCCA GCCAGGCCCC GCTGGACCTC CGGGTCCTGT GGGTCCCGCA GGTGGTCCTG 
     1921 GCTTCCCTGG TGCTCCTGGT GCCAAGGGCG AAGCTGGTCC CACTGGTGCT CGCGGTCCTG 
     1981 AAGGTGCTCA AGGTTCTCGT GGCGAGCCTG GCAATCCTGG GTCCCCTGGG CCTGCAGGTG 
     2041 CTTCTGGTAA CCCAGGGACT GATGGTATTC CTGGAGCCAA AGGATCCGCT GGTGCTCCTG 
     2101 GAATTGCTGG TGCCCCTGGC TTCCCTGGGC CCCGTGGCCC TCCCGGTCCT CAAGGTGCAA 
     2161 CTGGTCCCCT TGGCCCCAAA GGTCAGGCGG GTGAACCTGG CATTGCTGGC TTTAAAGGTG 
     2221 ATCAAGGCCC CAAGGGAGAG ACTGGACCTG CTGGGCCCCA AGGAGCCCCT GGCCCCGCTG 
     2281 GTGAAGAAGG CAAACGAGGT GCTCGAGGAG AGCCGGGTGG TGCTGGACCA ATCGGACCCC 
     2341 CTGGAGAGAG AGGTGCTCCT GGCAACCGTG GATTCCCAGG TCAAGATGGT CTGGCAGGTC 
     2401 CCAAGGGTGC CCCTGGAGAG CGAGGGCCCA GTGGCTTGAC TGGTCCCAAG GGAGCCAACG 
     2461 GTGACCCGGG TCGTCCTGGA GAACCTGGTC TTCCTGGAGC CAGGGGTCTT ACCGGTCGCC 
     2521 CTGGTGACGC TGGTCCTCAA GGCAAAGTTG GTCCTTCTGG AGCCCCTGGT GAAGACGGTC 
     2581 GCCCTGGACC TCCTGGTCCT CAGGGAGCTC GTGGGCAGCC TGGCGTCATG GGTTTCCCTG 
     2641 GCCCCAAAGG TGCCAACGGC GAGCCTGGCA AAGCTGGTGA GAAGGGTCTG GCTGGCGCTC 
     2701 CTGGTCTGAG AGGTCTTCCT GGAAAAGACG GTGAGACGGG AGCCGCAGGA CCCCCCGGCC 
     2761 CCAGTGGACC TGCTGGTGAA CGAGGCGAGC AGGGCGCTCC TGGACCATCA GGGTTCCAGG 
     2821 GACTTCCTGG CCCTCCCGGT CCCCCAGGTG AAGGTGGAAA GCAAGGTGAC CAGGGTATTC 
     2881 CTGGTGAAGC TGGAGCTCCT GGCCTTGTGG GTCCTCGGGG CGAGCGAGGT TTCCCAGGTG 
     2941 AACGTGGCTC TCCTGGTGCT CAGGGCCTTC AGGGTCCCCG AGGCCTCCCT GGCACTCCTG 
     3001 GTACTGATGG TCCCAAAGGT GCAGCTGGCC CAGATGGCCC CCCTGGGGCT CAGGGGCCTC 
     3061 CAGGTCTACA GGGAATGCCT GGTGAGAGAG GAGCCGCTGG CATTGCTGGG CCCAAGGGAG 
     3121 ACAGAGGCGA TGTTGGCGAG AAAGGCCCAG AGGGAGCTCC TGGGAAGGAT GGCGGCCGAG 
     3181 GTCTGACTGG GCCCATCGGA CCCCCAGGCC CAGCAGGGGC CAACGGCGAG AAGGGAGAAG 
     3241 TCGGACCTCC TGGCCCGTCA GGAAGTACCG GAGCTCGAGG TGCCCCGGGT GAACGCGGAG 
     3301 AGACCGGGCC ACCTGGACCT GCTGGATTCG CTGGCCCTCC TGGTGCTGAT GGCCAGCCTG 
     3361 GTGCCAAGGG TGATCAAGGA GAAGCCGGAC AGAAAGGAGA TGCTGGTGCC CCCGGCCCAC 
     3421 AAGGCCCCTC GGGAGCCCCT GGGCCACAGG GTCCTACTGG AGTGACTGGT CCTAAGGGAG 
     3481 CCCGAGGTGC CCAAGGTCCC CCGGGAGCCA CCGGATTCCC TGGAGCTGCT GGCCGAGTTG 
     3541 GACCCCCAGG TGCTAATGGC AATCCTGGAC CCGCCGGTCC CCCTGGTCCT GCTGGAAAAG 
     3601 ATGGTCCCAA AGGTGTTCGA GGAGACAGTG GCCCCCCTGG CAGAGCTGGT GACCCCGGTC 
     3661 TTCAAGGTCC TGCAGGAGCT CCTGGCGAGA AAGGAGAACC TGGAGATGAT GGTCCCTCTG 
     3721 GTCTTGATGG TCCTCCAGGT CCCCAGGGGC TGGCTGGTCA AAGGGGCATT GTTGGTCTGC 
     3781 CTGGTCAGCG TGGTGAGAGA GGATTCCCCG GCCTTCCCGG CCCATCGGGT GAGCCCGGCA 
     3841 AGCAGGGTGC ACCTGGCGCG TCTGGAGACA GAGGTCCTCC TGGTCCTGTG GGGCCTCCTG 
     3901 GCCTGACAGG GCCTGCAGGT GAACCTGGAC GAGAGGGCAG CCCTGGTGCT GATGGACCCC 
     3961 CTGGAAGAGA TGGTGCAGCT GGAGTCAAGG GAGATCGTGG TGAGACTGGA GCACTGGGTG 
     4021 CCCCTGGAGC TCCTGGGCCC CCAGGCTCTC CTGGTCCTGC TGGCCCAACT GGCAAACAAG 
     4081 GAGACAGAGG AGAGGCTGGT GCACAAGGTC CTATGGGTCC CTCAGGACCT GCTGGAGCCC 
     4141 GTGGGATTGC AGGCCCTCAA GGCCCCCGAG GTGACAAACG AGAATCTGGA GAGCAGGGCG 
     4201 AGAGGGGACT GAAGGGACAC CGAGGTTTCA CTGGACTGCA GGGTCTGCCT GGCCCTCCGG 
     4261 GTCCTTCTGG AGATCAGGGT GCTTCTGGCC CTGCTGGTCC TTCTGGCCCT AGAGGTCCAC 
     4321 CTGGCCCTGT TGGTCCCTCT GGCAAAGATG GCTCTAATGG AATCCCTGGC CCCATCGGGC 
     4381 CTCCAGGTCC CCGTGGACGC TCAGGAGAAA CAGGCCCTGT TGGTCCCCCT GGAAGTCCCG 
     4441 GTCCTCCTGG CCCTCCAGGT CCTCCTGGTC CTGGCATCGA CATGTCAGCC TTTGCTGGCT 
     4501 TAGGGCAGAG AGAGAAGGGC CCCGACCCCA TGCAGTACAT GCGGGCCGAC GAGGCAGACA 
     4561 GTACCTTGAG ACAGCACGAC GTGGAGGTGG ACGCTACACT CAAGTCACTG AACAACCAGA 
     4621 TTGAGAGCAT CCGCAGCCCC GACGGCTCCC GCAAGAACCC TGCTCGCACT TGCCAAGACC 
     4681 TGAAACTCTG CCACCCCGAG TGGAAGAGCG GAGACTACTG GATTGATCCC AACCAGGGCT 
     4741 GCACCTTGGA CGCCATGAAA GTTTTCTGCA ACATGGAGAC CGGCGAGACT TGCGTCTACC 
     4801 CCAACCCAGC GACTGTCCCT CGGAAAAACT GGTGGAGCAG CAAGAGCAAG GAAAAGAAAC 
     4861 ACATCTGGTT TGGAGAGACC ATGAACGGTG GCTTCCACTT CAGCTATGGC GATGGCAACC 
     4921 TGGCTCCCAA CACCGCTAAC GTCCAGATGA CTTTCCTCCG TCTACTGTCC ACTGAGGGCT 
     4981 CCCAGAACAT CACCTACCAC TGTAAGAACA GCATCGCCTA CCTGGACGAA GCGGCTGGCA 
     5041 ACCTCAAGAA GGCCTTGCTC ATCCAGGGCT CCAATGATGT AGAGATGAGG GCCGAGGGCA 
     5101 ACAGCAGGTT CACATACACT GCCCTGAAGG ATGGCTGCAC GAAACACACT GGTAAGTGGG 
     5161 GCAAGACCGT CATCGAGTAC CGATCACAGA AGACCTCCCG CCTTCCCATT ATTGACATCG 
     5221 CACCCATGGA CATTGGAGGG GCTGAACAGG AATTTGGTGT GGACATAGGG CCTGTCTGCT 
     5281 TCTTGACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     5341 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     5401 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     5461 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     5521 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     5581 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     5641 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     5701 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     5761 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     5821 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     5881 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     5941 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     6001 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     6061 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     6121 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     6181 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     6241 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     6301 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     6361 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     6421 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     6481 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     6541 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     6601 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     6661 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     6721 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     6781 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     6841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     6901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     6961 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     7021 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     7081 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     7141 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     7201 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     7261 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     7321 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     7381 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     7441 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     7501 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     7561 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     7621 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     7681 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     7741 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     7801 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     7861 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     7921 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     7981 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     8041 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     8101 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     8161 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     8221 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     8281 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     8341 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     8401 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     8461 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     8521 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     8581 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     8641 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     8701 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     8761 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     8821 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     8881 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     8941 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     9001 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     9061 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     9121 AAATTTAACG CGAATTTT
//