LOCUS       MR227542                7596 bp    DNA     circular  SYN 
DEFINITION  Tlr3 (Myc-DDK-tagged) - Mouse toll-like receptor 3 (Tlr3)
ACCESSION   NM_126166
VERSION     NM_126166.4
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3753..3844 
                     /label="Myc-DDK"  
     misc_feature    3874..4459 
                     /label="PolyA signal"  
     misc_feature    3931..3950 
                     /label="Restriction Enzyme"  
     misc_feature    4735..5407 
                     /label="ColE1"  
     misc_feature    5837..6631 
                     /label="Kan/Neo"  
     misc_feature    6666..6968 
                     /label="SV40 ori"  
     misc_feature    7054..7531 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3735..3741 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3735 
                     /label="Tlr3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAAGGGTGT TCCTCTTATC TAATGTACTC CTTTGGGGGA CTTTTGTCCC 
     1081 TATGGATTCT TCTGGTGTCT TCCACAAACC AATGCACTGT GAGATACAAC GTAGCTGACT 
     1141 GCAGCCATTT GAAGCTAACA CACATACCTG ATGATCTTCC CTCTAACATA ACAGTGTTGA 
     1201 ATCTTACTCA CAACCAACTC AGAAGATTAC CACCTACCAA CTTTACAAGA TACAGCCAAC 
     1261 TTGCTATCTT GGATGCAGGA TTTAACTCCA TTTCAAAACT GGAGCCAGAA CTGTGCCAAA 
     1321 TACTCCCTTT GTTGAAAGTA TTGAACCTGC AACATAATGA GCTCTCTCAG ATTTCTGATC 
     1381 AAACCTTTGT CTTCTGCACG AACCTGACAG AACTCGATCT AATGTCTAAC TCAATACACA 
     1441 AAATTAAAAG CAACCCTTTC AAAAACCAGA AGAATCTAAT CAAATTAGAT TTGTCTCATA 
     1501 ATGGTTTATC ATCTACAAAG TTGGGAACGG GGGTCCAACT GGAGAACCTC CAAGAACTGC 
     1561 TCTTAGCAAA AAATAAAATC CTTGCGTTGC GAAGTGAAGA ACTTGAGTTT CTTGGCAATT 
     1621 CTTCTTTACG AAAGTTGGAC TTGTCATCAA ATCCACTTAA AGAGTTCTCC CCGGGGTGTT 
     1681 TCCAGACAAT TGGCAAGTTA TTCGCCCTCC TCTTGAACAA CGCCCAACTG AACCCCCACC 
     1741 TCACAGAGAA GCTTTGCTGG GAACTTTCAA ACACAAGCAT CCAGAATCTC TCTCTGGCTA 
     1801 ACAACCAGCT GCTGGCCACC AGCGAGAGCA CTTTCTCTGG GCTGAAGTGG ACAAATCTCA 
     1861 CCCAGCTCGA TCTTTCCTAC AACAACCTCC ATGATGTCGG CAACGGTTCC TTCTCCTATC 
     1921 TCCCAAGCCT GAGGTATCTG TCTCTGGAGT ACAACAATAT ACAGCGTCTG TCCCCTCGCT 
     1981 CTTTTTATGG ACTCTCCAAC CTGAGGTACC TGAGTTTGAA GCGAGCATTT ACTAAGCAAA 
     2041 GTGTTTCACT TGCTTCACAT CCCAACATTG ACGATTTTTC CTTTCAATGG TTAAAATATT 
     2101 TGGAATATCT CAACATGGAT GACAATAATA TTCCAAGTAC CAAAAGCAAT ACCTTCACGG 
     2161 GATTGGTGAG TCTGAAGTAC CTAAGTCTTT CCAAAACTTT CACAAGTTTG CAAACTTTAA 
     2221 CAAATGAAAC ATTTGTGTCA CTTGCTCATT CTCCCTTGCT CACTCTCAAC TTAACGAAAA 
     2281 ATCACATCTC AAAAATAGCA AATGGTACTT TCTCTTGGTT AGGCCAACTC AGGATACTTG 
     2341 ATCTCGGCCT TAATGAAATT GAACAAAAAC TCAGCGGCCA GGAATGGAGA GGTCTGAGAA 
     2401 ATATATTTGA GATCTACCTA TCCTATAACA AATACCTCCA ACTGTCTACC AGTTCCTTTG 
     2461 CATTGGTCCC CAGCCTTCAA AGACTGATGC TCAGGAGGGT GGCCCTTAAA AATGTGGATA 
     2521 TCTCCCCTTC ACCTTTCCGC CCTCTTCGTA ACTTGACCAT TCTGGACTTA AGCAACAACA 
     2581 ACATAGCCAA CATAAATGAG GACTTGCTGG AGGGTCTTGA GAATCTAGAA ATCCTGGATT 
     2641 TTCAGCACAA TAACTTAGCC AGGCTCTGGA AACGCGCAAA CCCCGGTGGT CCCGTTAATT 
     2701 TCCTGAAGGG GCTGTCTCAC CTCCACATCT TGAATTTAGA GTCCAACGGC TTAGATGAAA 
     2761 TCCCAGTCGG GGTTTTCAAG AACTTATTCG AACTAAAGAG CATCAATCTA GGACTGAATA 
     2821 ACTTAAACAA ACTTGAACCA TTCATTTTTG ATGACCAGAC ATCTCTAAGG TCACTGAACC 
     2881 TCCAGAAGAA CCTCATAACA TCTGTTGAGA AGGATGTTTT CGGGCCGCCT TTTCAAAACC 
     2941 TGAACAGTTT AGATATGCGC TTCAATCCGT TCGACTGCAC GTGTGAAAGT ATTTCCTGGT 
     3001 TTGTTAACTG GATCAACCAG ACCCACACTA ATATCTCTGA GCTGTCCACT CACTACCTCT 
     3061 GTAACACTCC ACATCATTAT TATGGCTTCC CCCTGAAGCT TTTCGATACA TCATCCTGTA 
     3121 AAGACAGCGC CCCCTTTGAA CTCCTCTTCA TAATCAGCAC CAGTATGCTC CTGGTTTTTA 
     3181 TACTTGTGGT ACTGCTCATT CACATCGAGG GCTGGAGGAT CTCTTTTTAC TGGAATGTTT 
     3241 CAGTGCATCG GATTCTTGGT TTCAAGGAAA TAGACACACA GGCTGAGCAG TTTGAATATA 
     3301 CAGCCTACAT AATTCATGCC CATAAAGACA GAGACTGGGT CTGGGAACAT TTCTCCCCAA 
     3361 TGGAAGAACA AGACCAATCT CTCAAATTTT GCCTAGAAGA AAGGGACTTT GAAGCAGGCG 
     3421 TCCTTGGACT TGAAGCAATT GTTAATAGCA TCAAAAGAAG CCGAAAAATC ATTTTCGTTA 
     3481 TCACACACCA TTTATTAAAA GACCCTCTGT GCAGAAGATT CAAGGTACAT CACGCAGTTC 
     3541 AGCAAGCTAT TGAGCAAAAT CTGGATTCAA TTATACTGAT TTTTCTCCAG AATATTCCAG 
     3601 ATTATAAACT AAACCATGCA CTCTGTTTGC GAAGAGGAAT GTTTAAATCT CATTGCATCT 
     3661 TGAACTGGCC AGTTCAGAAA GAACGGATAA ATGCCTTTCA TCATAAATTG CAAGTAGCAC 
     3721 TTGGATCTCG GAATTCAGCA CATACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     3781 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     3841 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     3901 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     3961 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     4021 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     4081 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     4141 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     4201 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     4261 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     4321 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     4381 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     4441 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     4501 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     4561 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     4621 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     4681 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     4741 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     4801 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     4861 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     4921 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     4981 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     5041 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     5101 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     5161 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     5221 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     5281 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     5341 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     5401 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     5461 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     5521 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     5581 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     5641 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     5701 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     5761 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     5821 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     5881 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     5941 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     6001 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     6061 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     6121 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     6181 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     6241 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     6301 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     6361 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     6421 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     6481 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     6541 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     6601 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     6661 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     6721 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     6781 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     6841 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     6901 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     6961 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     7021 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     7081 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     7141 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     7201 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     7261 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     7321 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     7381 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     7441 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     7501 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     7561 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//