LOCUS       MR227515                7461 bp    DNA     circular  SYN 
DEFINITION  Eln (Myc-DDK-tagged) - Mouse elastin (Eln)
ACCESSION   NM_007925
VERSION     NM_007925.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3618..3709 
                     /label="Myc-DDK"  
     misc_feature    3739..4324 
                     /label="PolyA signal"  
     misc_feature    3796..3815 
                     /label="Restriction Enzyme"  
     misc_feature    4600..5272 
                     /label="ColE1"  
     misc_feature    5702..6496 
                     /label="Kan/Neo"  
     misc_feature    6531..6833 
                     /label="SV40 ori"  
     misc_feature    6919..7396 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3600..3606 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3600 
                     /label="Eln"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGGTCTG ACAGCGGTAG TCCCGCAGCC TGGCGTCTTG CTGATCCTCT 
     1081 TGCTCAACCT CCTCCATCCC GCGCAGCCTG GAGGGGTTCC AGGAGCTGTG CCTGGCGGAC 
     1141 TTCCTGGTGG AGTTCCCGGT GGAGTCTATT ATCCAGGGGC TGGTATTGGA GGCCTGGGAG 
     1201 GAGGAGGAGG AGCTCTGGGA CCTGGAGGAA AACCACCTAA GCCAGGTGCC GGACTTCTGG 
     1261 GAACGTTTGG AGCAGGTCCT GGAGGACTTG GAGGTGCTGG CCCGGGTGCA GGTCTCGGGG 
     1321 CCTTTCCTGC AGGCACCTTC CCAGGGGCAG GAGCTCTGGT GCCCGGGGGA GCAGCAGGGG 
     1381 CTGCTGCGGC TTATAAAGCT GCCGCCAAAG CTGGGGCTGG GCTTGGTGGC GTTGGCGGAG 
     1441 TCCCAGGTGG TGTTGGCGTT GGTGGAGTTC CAGGTGGTGT TGGAGTTGGC GGAGTCCCAG 
     1501 GTGGTGTTGG AGTTGGTGGA GTCCCTGGCG GTGTTGGTGG TATTGGTGGC ATCGGTGGCT 
     1561 TAGGAGTCTC GACAGGTGCT GTGGTGCCCC AAGTCGGAGC TGGCATCGGA GCTGGAGGAA 
     1621 AGCCTGGGAA AGTTCCTGGT GTTGGTCTTC CAGGTGTATA CCCAGGCGGA GTGCTCCCAG 
     1681 GAACAGGAGC TCGGTTCCCT GGTGTGGGGG TGCTCCCTGG AGTTCCCACT GGCACAGGAG 
     1741 TCAAAGCCAA GGCTCCAGGT GGAGGTGGTG CTTTTGCTGG AATCCCAGGG GTCGGACCCT 
     1801 TTGGGGGTCA GCAGCCTGGT GTCCCACTGG GTTATCCCAT CAAAGCACCA AAGCTGCCAG 
     1861 GTGGCTACGG ACTGCCCTAT ACCAATGGGA AATTGCCCTA TGGAGTAGCT GGTGCAGGGG 
     1921 GCAAGGCTGG CTACCCAACA GGGACAGGGG TCGGATCCCA GGCGGCGGCG GCAGCAGCTA 
     1981 AAGCAGCCAA GTATGGTGCT GGGGGAGCTG GAGTCCTCCC TGGTGTTGGA GGGGGTGGCA 
     2041 TTCCTGGTGG TGCTGGCGCA ATTCCTGGAA TTGGAGGCAT TGCAGGCGCT GGAACTCCTG 
     2101 CAGCAGCAGC TGCTGCAAAG GCTGCTGCTA AGGCTGCTAA GTATGGAGCT GCTGGAGGTT 
     2161 TAGTGCCTGG TGGACCAGGA GTTAGGCTCC CAGGTGCTGG AATCCCAGGT GTTGGTGGCA 
     2221 TTCCTGGTGT TGGTGGCATC CCAGGTGTTG GGGGCCCTGG TATTGGAGGT CCAGGCATTG 
     2281 TGGGTGGACC AGGAGCTGTG TCACCAGCTG CAGCTGCTAA AGCTGCTGCC AAAGCTGCCA 
     2341 AATACGGAGC CAGAGGTGGA GTTGGCATCC CGACATATGG GGTTGGTGCT GGTGGCTTTC 
     2401 CTGGCTATGG TGTTGGAGCT GGAGCAGGAC TTGGAGGTGC AAGCCCAGCT GCTGCTGCTG 
     2461 CCGCCGCCAA AGCTGCTAAG TATGGTGCTG GAGGAGCTGG AGCCCTGGGA GGCCTGGTGC 
     2521 CAGGTGCAGT ACCAGGTGCA CTGCCAGGTG CAGTACCAGC TGTGCCGGGA GCTGGTGGAG 
     2581 TGCCAGGAGC AGGTACCCCT GCAGCTGCAG CTGCTGCCGC CGCCGCTAAA GCAGCCGCCA 
     2641 AAGCAGGTTT GGGTCCTGGT GTTGGTGGGG TTCCTGGTGG AGTTGGTGTT GGTGGGATTC 
     2701 CCGGTGGAGT TGGTGTTGGT GGGGTTCCTG GTGGAGTTGG CCCTGGTGGT GTTACTGGTA 
     2761 TTGGAGCTGG TCCTGGCGGT CTTGGAGGAG CAGGGTCACC GGCTGCCGCT AAATCTGCTG 
     2821 CTAAGGCAGC TGCCAAAGCC CAGTACAGAG CTGCCGCTGG GCTTGGAGCT GGTGTCCCTG 
     2881 GATTTGGGGC TGGTGCTGGT GTCCCCGGAT TTGGGGCTGG TGCTGGTGTC CCCGGATTTG 
     2941 GGGCTGGTGC TGGTGTCCCC GGATTTGGGG CTGGTGCTGG TGTCCCTGGA TTTGGAGCTG 
     3001 GAGCAGTACC TGGATCGCTG GCTGCATCCA AAGCTGCTAA ATATGGAGCA GCAGGTGGCC 
     3061 TTGGTGGCCC TGGAGGTCTC GGTGGCCCTG GAGGTCTCGG TGGACCTGGA GGACTTGGTG 
     3121 GGGCTGGTGT TCCCGGTAGA GTAGCAGGAG CTGCACCCCC TGCTGCTGCC GCTGCTGCTG 
     3181 CCAAAGCTGC TGCTAAGGCT GCCCAGTATG GCCTTGGTGG AGCCGGAGGA TTGGGAGCCG 
     3241 GTGGACTGGG GGCCGGTGGA CTGGGAGCCG GTGGACTGGG AGCTGGTGGA CTGGGAGCCG 
     3301 GTGGACTGGG AGCTGGTGGA CTGGGAGCCG GTGGACTGGG AGCTGGTGGA GGTGTGTCCC 
     3361 CTGCTGCAGC TGCTAAGGCA GCCAAATATG GTGCTGCTGG CCTTGGAGGT GTCCTAGGAG 
     3421 CCAGGCCATT CCCAGGTGGA GGAGTTGCAG CAAGACCTGG CTTTGGACTT TCTCCCATTT 
     3481 ATCCAGGTGG TGGTGCTGGG GGCCTGGGAG TTGGTGGAAA ACCCCCGAAG CCCTATGGAG 
     3541 GAGCCCTTGG AGCCCTGGGA TACCAAGGTG GGGGCTGCTT TGGGAAATCC TGTGGCCGGA 
     3601 AGAGAAAGAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     3661 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     3721 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     3781 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     3841 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     3901 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     3961 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     4021 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     4081 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     4141 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     4201 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     4261 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     4321 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     4381 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     4441 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     4501 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     4561 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     4621 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     4681 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     4741 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     4801 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     4861 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     4921 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     4981 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     5041 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     5101 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     5161 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     5221 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     5281 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     5341 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     5401 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     5461 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     5521 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     5581 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     5641 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     5701 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     5761 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     5821 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     5881 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     5941 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     6001 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     6061 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     6121 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     6181 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     6241 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     6301 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     6361 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     6421 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     6481 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     6541 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     6601 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     6661 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     6721 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     6781 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     6841 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     6901 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     6961 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     7021 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     7081 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     7141 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     7201 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     7261 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     7321 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     7381 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     7441 CAAAAATTTA ACGCGAATTT T
//