LOCUS       MR227512                8469 bp    DNA     circular  SYN 
DEFINITION  Hipk2 (Myc-DDK-tagged) - Mouse homeodomain interacting protein kinase 2 (Hipk2), transcript variant 2
ACCESSION   NM_010433
VERSION     NM_010433.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4626..4717 
                     /label="Myc-DDK"  
     misc_feature    4747..5332 
                     /label="PolyA signal"  
     misc_feature    4804..4823 
                     /label="Restriction Enzyme"  
     misc_feature    5608..6280 
                     /label="ColE1"  
     misc_feature    6710..7504 
                     /label="Kan/Neo"  
     misc_feature    7539..7841 
                     /label="SV40 ori"  
     misc_feature    7927..8404 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4608..4614 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4608 
                     /label="Hipk2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCCCCGTG TACGAAGGTA TGGCCTCACA TGTGCAAGTT TTCTCCCCTC 
     1081 ACACCCTTCA ATCAAGTGCC TTCTGTAGTG TGAAGAAACT AAAAGTAGAG CCAAGTTCCA 
     1141 ACTGGGACAT GACTGGGTAC GGCTCCCACA GCAAAGTGTA CAGCCAGAGC AAGAACATAC 
     1201 CACCTTCTCA GCCAGCCTCC ACAACCGTCA GCACCTCCTT GCCAATCCCC AACCCAAGCC 
     1261 TACCTTACGA GCAGACCATC ATCTTCCCAG GAAGTACTGG ACACATAGTT GTAACATCAG 
     1321 CAAGTAGCAC TTCTGTCACC GGGCAAGTCC TCGGTGGACC ACATAACCTA ATGCGTCGAA 
     1381 GCACTGTGAG CCTCCTTGAC ACCTACCAAA AATGTGGACT CAAGCGCAAG AGTGAGGAGA 
     1441 TTGAGAACAC AAGCAGCGTG CAGATCATTG AAGAGCATCC ACCCATGATT CAGAATAATG 
     1501 CCAGCGGGGC CACTGTAGCC ACTGCCACCA CATCCACTGC CACCTCCAAA AACAGTGGCT 
     1561 CCAACAGTGA AGGGGACTAT CAGCTGGTGC AGCATGAAGT ACTGTGCTCC ATGACCAACA 
     1621 CCTATGAGGT TTTAGAGTTC TTGGGGCGAG GGACATTTGG GCAAGTGGTC AAGTGCTGGA 
     1681 AACGGGGCAC CAATGAAATT GTGGCCATTA AGATCCTCAA GAACCACCCC TCCTATGCCC 
     1741 GGCAAGGCCA GATTGAAGTG AGCATCTTGG CCCGGCTGAG CACGGAGAGC GCTGATGACT 
     1801 ACAATTTTGT GCGGGCCTAT GAGTGCTTCC AGCACAAGAA CCACACGTGC TTAGTCTTTG 
     1861 AGATGTTGGA GCAGAACCTT TATGACTTTC TGAAACAGAA CAAGTTTAGT CCCTTGCCCC 
     1921 TCAAGTATAT TCGCCCAGTC CTCCAGCAGG TAGCCACAGC CCTGATGAAA CTCAAAAGCC 
     1981 TGGGTCTTAT CCATGCTGAC CTCAAACCAG AAAACATCAT GCTGGTGGAT CCATCCAGAC 
     2041 AACCGTACCG AGTGAAGGTC ATTGACTTTG GTTCAGCTAG TCATGTGTCC AAAGCTGTCT 
     2101 GCTCTACGTA CTTGCAATCC AGATACTACC GGGCCCCTGA GATCATCCTT GGTTTGCCAT 
     2161 TCTGTGAGGC AATTGACATG TGGTCCCTTG GTTGTGTCAT TGCTGAACTG TTCCTGGGCT 
     2221 GGCCATTATA CCCAGGTGCT TCTGAGTATG ATCAGATTCG GTATATTTCA CAAACACAGG 
     2281 GTTTACCAGC TGAATATTTA TTAAGTGCAG GGACAAAGAC AACTAGGTTT TTCAACCGTG 
     2341 ACACAGACTC ACCGTATCCT TTGTGGAGGC TAAAGACACC AGATGACCAT GAAGCAGAGA 
     2401 CGGGAATTAA GTCAAAGGAA GCAAGAAAGT ACATTTTCAA CTGTTTGGAT GATATGGCCC 
     2461 AGGTGAACAT GACAACAGAT TTGGAAGGGA GTGACATGTT AGTAGAGAAG GCAGACCGAC 
     2521 GGGAGTTCAT TGACCTGTTA AAGAAGATGC TGACCATCGA TGCTGATAAG AGAGTCACTC 
     2581 CCATTGAAAC TCTGAACCAC CCCTTTGTCA CCATGACACA CCTGCTTGAC TTCCCCCATA 
     2641 GTGCCCATGT CAAATCTTGT TTCCAAAACA TGGAGATTTG CAAGCGCCGG GTGAATATGT 
     2701 ATGACACAGT GAACCAGAGC AAAACACCTT TCATCACTCA CGTGGCCCCC AGCACATCCA 
     2761 CCAACTTGAC CATGACCTTT AACAACCAGC TGACCACTGT CCACAACCAG GCTCCCACCA 
     2821 CCTCCAGTGC CACTCTTTCC TTAGCCAATC CCGAAGTCTC CATACTAAAC TACCAATCTG 
     2881 CACTCTACCA GCCCTCAGCG GCATCCATGG CCGCGGTGGC CCCGCGGAGC ATGCCCCTGC 
     2941 AGACGGGAAC AGCCCAAATT TGTGCCCGAC CTGATCCCTT CCAGCAAGCT CTCATCGTGT 
     3001 GTCCCCCTGG CTTCCAAGGC CTGCAGGCCT CTCCCTCCAA GCACGCTGGC TACTCAGTGC 
     3061 GGATGGAAAA TGCTGTCCCC ATCGTCACCC AGGCGCCAGG AGCTCAGCCT CTTCAGATCC 
     3121 AACCAGGCCT GCTTGCTCAG CAGGCCTGGC CAGGTGGGGC CCAACAGATC CTACTTCCTC 
     3181 CAGCATGGCA GCAGCTGACT GGCGTGGCCA CCCACACATC TGTACAGCAT GCAGCTGTGA 
     3241 TTCCTGAGAC CATGGCAGGC ACTCAGCAAC TGGCTGACTG GAGGAACACG CATGCTCATG 
     3301 GAAGTCATTA CAATCCCATC ATGCAGCAGC CTGCACTCTT GACTGGTCAT GTGACCCTTC 
     3361 CAGCTGCCCA GCCCTTAAAT GTGGGTGTAG CCCATGTGAT GAGGCAACAA CCAACCAGCA 
     3421 CCACCTCCTC CAGGAAGAGT AAGCAGCACC AGTCATCTGT GAGAAATGTC TCCACCTGTG 
     3481 AGGTGACCTC TTCACAGGCT ATCAGCTCCC CTCAGCGATC CAAGCGTGTC AAGGAGAACA 
     3541 CTCCCCCACG GTGCGCCATG GTACACAGCA GCCCAGCTTG CAGCACCTCA GTCACCTGTG 
     3601 GGTGGGGCGA CGTGGCCTCC AGCACCACCC GGGAGCGACA GCGGCAGACG ATTGTCATCC 
     3661 CCGACACCCC GAGCCCCACA GTCAGTGTCA TCACCATCAG CAGTGACACC GATGAAGAAG 
     3721 AGGAGCAGAA GCACGCCCCC ACCAGCACAG TCTCCAAGCA AAGAAAAAAT GTCATCAGCT 
     3781 GTGTCACCGT CCACGACTCT CCCTACTCTG ACTCCTCCAG CAACACCAGC CCCTACTCGG 
     3841 TGCAGCAGCG CACAGGGCAC AACGGCACCA ACACCTTGGA CACCAAGGGG GGCCTAGAGA 
     3901 ATCACTGCAC TGGCAACCCC CGCACCATCA TCGTACCCCC ACTGAAGACC CAGGCCAGTG 
     3961 AAGTGTTGGT AGAATGTGAC AGCCTAGGGC CAGCGATCAG TGCCAGTCAC CATTCGTCCT 
     4021 CCTTCAAGTC CAAGTCCTCC AGCACCGTGA CCTCCACCAG TGGACACTCT TCAGGGAGCT 
     4081 CTTCAGGAGC CATCGCCTAC CGTCAGCAGC GGCCAGGCCC CCACTTCCAG CAGCAGCAGC 
     4141 CCCTCAATCT CAGCCAGGCC CAGCAGCACA TGGCTGCGGA CCGCACCGGG AGTCACCGTC 
     4201 GACAGCAGGC CTACATCACT CCGACCATGG CGCAAGCTCC GTACACCTTC CCGCACAACA 
     4261 GCCCCAGCCA TGGCACTGTT CACCCCCACC TGGCTGCGGC TGCCCACCTC CCCACCCAGC 
     4321 CTCACCTCTA CACCTATACA GCGCCCACAG CCCTAGGCTC CACCGGCACT GTAGCCCACC 
     4381 TGGTGGCATC CCAAGGCTCA GCACGCCACA CCGTGCAGCA CACTGCCTAT CCGGCCAGCA 
     4441 TCGTCCACCA GGTCCCTGTG AGCATGGGGC CCCGGGTCCT GCCCTCGCCC ACCATCCACC 
     4501 CCAGTCAGTA TCCAGCCCAG TTTGCCCACC AGACCTATAT CAGCGCCTCG CCAGCCTCCA 
     4561 CCGTCTACAC TGGATACCCA CTGAGTCCTG CCAAGGTCAA CCAATACCCT TACATAACGC 
     4621 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     4681 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     4741 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     4801 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     4861 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     4921 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     4981 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     5041 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     5101 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     5161 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     5221 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     5281 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     5341 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     5401 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     5461 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     5521 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     5581 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     5641 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     5701 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     5761 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     5821 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     5881 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     5941 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     6001 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     6061 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     6121 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     6181 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     6241 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     6301 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     6361 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     6421 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     6481 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     6541 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     6601 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     6661 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     6721 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     6781 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     6841 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     6901 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     6961 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     7021 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     7081 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     7141 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     7201 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     7261 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     7321 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     7381 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     7441 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     7501 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     7561 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     7621 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     7681 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     7741 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     7801 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7861 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     7921 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     7981 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     8041 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     8101 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     8161 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     8221 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     8281 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     8341 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     8401 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     8461 GCGAATTTT
//