LOCUS       MR227483                8082 bp    DNA     circular  SYN 
DEFINITION  Sall4 (Myc-DDK-tagged) - Mouse sal-like 4 (Drosophila) (Sall4), transcript variant a
ACCESSION   NM_175303
VERSION     NM_175303.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4239..4330 
                     /label="Myc-DDK"  
     misc_feature    4360..4945 
                     /label="PolyA signal"  
     misc_feature    4417..4436 
                     /label="Restriction Enzyme"  
     misc_feature    5221..5893 
                     /label="ColE1"  
     misc_feature    6323..7117 
                     /label="Kan/Neo"  
     misc_feature    7152..7454 
                     /label="SV40 ori"  
     misc_feature    7540..8017 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4221..4227 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4221 
                     /label="Sall4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCGAGGCGC AAGCAGGCGA AGCCCCAGCA CATCAACTGG GAGGAGGGCC 
     1081 AGGGCGAGCA GCCTCAGCAG CTACCGAGCC CCGACCTCGC CGAGGCGCTG GCGGCGGAGG 
     1141 AACCCGGTGC TCCAGTGAAC TCCCCTGGGA ACTGCGATGA AGCCTCAGAG GACTCCATAC 
     1201 CGGTGAAGCG GCCCCGGCGG GAGGACACTC ACATCTGCAA CAAATGCTGT GCCGAGTTCT 
     1261 TTAGTCTCTC TGAATTCATG GAACACAAGA AAAGTTGCAC TAAAACCCCT CCTGTCCTCA 
     1321 TCATGAATGA CAGCGAGGGG CCAGTGCCTT CAGAGGACTT TTCCAGAGCT GCCCTGAGCC 
     1381 ACCAGCTGGG CAGCCCAAGC AATAAAGACA GTCTCCAGGA GAACGGCAGC AGCTCGGGGG 
     1441 ACTTGAAGAA GCTGGGCACG GACTCCATCC TGTACTTGAA GACAGAGGCT ACCCAGCCAT 
     1501 CCACACCCCA GGACATAAGC TATTTACCCA AAGGCAAAGT AGCCAACACC AATGTGACTC 
     1561 TGCAGGCGCT CCGCGGCACC AAGGTGGCCG TGAACCAACG GGGTGCAGAG GCACCCATGG 
     1621 CGCCCATGCC TGCTGCCCAA GGCATCCCTT GGGTCCTGGA GCAGATCCTG TGCCTGCAGC 
     1681 AGCAGCAACT CCAGCAAATC CAGCTTACGG AACAGATTCG CGTCCAGGTG AACATGTGGG 
     1741 CAGCGCACGC GCTCCACTCT GGAGTGGCGG GGGCCGACAC GCTGAAGGCC TTAAGCAGCC 
     1801 ATGTGTCTCA GCAAGTGTCC GTGTCCCAGC AGGTGTCGGC TGCCGTGGCC CTGCTCAGCC 
     1861 AGAAAGCCTC AAACCCAGCT CTGTCGCTCG ATGCCTTGAA ACAAGCCAAG CTACCTCATG 
     1921 CCAGCGTCCC CTCCGCAGCC AGCCCGTTGT CCTCGGGGTT AACGTCCTTC ACCTTGAAGC 
     1981 CTGACGGGAC ACGGGTTCTC CCCAACTTCG TGTCTCGCCT TCCCAGTGCC CTGCTACCTC 
     2041 AGACTCCGGG CTCTGTGCTC CTGCAGAGTC CCTTCTCCGC TGTGACGCTC GACCAGTCCA 
     2101 AGAAAGGAAA GGGGAAACCC CAGAACCTCT CCGCCTCTGC CTCGGTGTTA GATGTCAAGG 
     2161 CCAAGGACGA AGTCGTCCTC GGTAAGCACA AGTGTAGGTA CTGTCCCAAG GTTTTCGGGA 
     2221 CAGATAGCTC CCTTCAGATT CACCTTCGCT CCCACACCGG AGAGAGACCT TACGTGTGCC 
     2281 CTATCTGTGG TCACCGCTTC ACCACCAAGG GCAATCTCAA GGTCCACTTA CAACGACACC 
     2341 CTGAGGTGAA GGCAAACCCC CAGCTGTTGG CCGAATTCCA GGACAAAGGG GCAGTGAGTG 
     2401 CCGCTTCTCA CTATGCACTC CCTGTCCCCG TCCCTGCCGA TGAATCGAGT CTCTCTGTAG 
     2461 ACGCCGAGCC TGTCCCGGTC ACGGGAACCC CTTCTCTAGG GCTACCTCAA AAGCTCACGT 
     2521 CAGGGCCTAA TTCCAGGGAC CTCATGGGTG GCTCCTTGCC CAATGACATG CAGCCAGGGC 
     2581 CTTCTCCAGA AAGTGAGGCG GGCCTTCCAC TCCTTGGGGT GGGGATGATA CATAATCCCC 
     2641 CAAAGGCTGG GGGCTTCCAG GGCACTGGGG CCCCAGAGTC AGGGTCCGAG ACCCTGAAAT 
     2701 TGCAGCAACT AGTGGAGAAC ATAGACAAGG CCACTACTGA CCCCAACGAG TGTCTCATTT 
     2761 GTCATCGGGT CCTCAGCTGT CAGAGTTCCC TGAAGATGCA TTACCGTACC CACACAGGGG 
     2821 AGAGACCATT CCAGTGCAAG ATCTGTGGCC GGGCCTTCTC CACCAAAGGC AACCTGAAGA 
     2881 CACACCTTGG GGTTCACCGA ACCAACACGA CCGTAAAGAC CCAACATTCG TGCCCCATCT 
     2941 GCCAGAAGAA ATTCACCAAC GCCGTCATGT TACAGCAGCA TATCCGGATG CACATGGGTG 
     3001 GCCAGATCCC CAACACCCCT CTGCCAGAGA GTCCCTGTGA CTTCACGGCT CCCGAGCCCG 
     3061 TGGCCGTCAG TGAGAATGGC AGTGCCAGCG GGGTCTGCCA GGACGACGCA GCAGAAGGGA 
     3121 TGGAAGCCGA GGAGGTCTGT TCTCAGGATG TTCCCAGTGG CCCCTCAACT GTCTCTCTGC 
     3181 CGGTTCCCAG TGCCCACCTG GCATCGCCCT CTCTGGGCTT CTCTGTGTTG GCCTCCCTGG 
     3241 ATACGCAGGG GAAAGGGGCT CTTCCGGCGC TGGCCCTGCA GAGGCAGAGC AGTCGAGAAA 
     3301 ACAGCTCCCT GGAGGGCGGT GACACTGGTC CAGCCAATGA CTCTTCCTTG CTCGTGGGTG 
     3361 ACCAGGAGTG TCAGAGCCGA AGCCCAGATG CCACGGAGAC CATGTGCTAC CAGGCAGTGT 
     3421 CACCTGCCAA TAGCCAAGCC GGAAGTGTCA AGTCCCGGTC TCCCGAGGGT CACAAGGCCG 
     3481 AGGGCGTGGA GAGCTGCCGC GTTGACACCG AAGGTCGTAC CAGCCTCCCT CCAACATTTA 
     3541 TCCGAGCACA GCCCACCTTT GTCAAAGTTG AAGTGCCTGG CACCTTTGTG GGACCCCCCA 
     3601 GCATGCCCTC GGGTATGCCG CCTTTGCTAG CATCGCAGCC GCAGCCACGC CGCCAGGCCA 
     3661 AGCAGCACTG CTGCACACGG TGTGGAAAGA ACTTCTCGTC TGCCAGTGCC CTGCAGATCC 
     3721 ACGAGCGAAC ACACACGGGA GAGAAGCCTT TCGTGTGTAA CATATGCGGG CGGGCCTTCA 
     3781 CCACGAAAGG CAACCTGAAG GTACACTACA TGACTCATGG GGCCAACAAT AACTCCGCCC 
     3841 GCCGGGGAAG GAAGCTGGCC ATAGAGAACC CCATGGCCGC GCTGAGTGCT GAGGGAAAGA 
     3901 GAGCGCCCGA GGTGTTTTCC AAGGAGCTCC TGTCCCCCGC GGTGAGTGTG GACCCCGCCT 
     3961 CCTGGAACCA GTACACCAGC GTCCTGAATG GGGGTCTGGC CATGAAGACC AACGAGATCT 
     4021 CCGTGATCCA GAGCGGAGGC ATCCCCACGC TGCCTGTGTC GCTGGGGGCC AGCTCTGTGG 
     4081 TGAGCAATGG CACGATTTCC AAGCTTGACG GCTCTCAGAC CGGTGTGAGC ATGCCCATGA 
     4141 GCGGGAACGG AGAAAAGCTC GCTGTTCCCG ACGGCATGGC CAAACACCAG TTCCCTCACT 
     4201 TCCTGGAGGA AAATAAGATT GCTGTCAGCA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     4261 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     4321 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     4381 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     4441 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     4501 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     4561 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     4621 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     4681 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     4741 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     4801 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     4861 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     4921 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     4981 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     5041 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     5101 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     5161 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     5221 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     5281 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     5341 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     5401 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     5461 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     5521 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     5581 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     5641 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     5701 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     5761 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     5821 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     5881 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     5941 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     6001 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     6061 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     6121 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     6181 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     6241 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     6301 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     6361 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     6421 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     6481 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     6541 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     6601 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     6661 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     6721 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     6781 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     6841 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     6901 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     6961 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     7021 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     7081 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     7141 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     7201 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     7261 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     7321 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7381 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7441 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     7501 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     7561 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     7621 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     7681 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     7741 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     7801 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     7861 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     7921 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     7981 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     8041 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//