LOCUS       MR227469                7818 bp    DNA     circular  SYN 
DEFINITION  Kit (Myc-DDK-tagged) - Mouse kit oncogene (Kit), transcript variant 1
ACCESSION   NM_001122733
VERSION     NM_001122733.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3975..4066 
                     /label="Myc-DDK"  
     misc_feature    4096..4681 
                     /label="PolyA signal"  
     misc_feature    4153..4172 
                     /label="Restriction Enzyme"  
     misc_feature    4957..5629 
                     /label="ColE1"  
     misc_feature    6059..6853 
                     /label="Kan/Neo"  
     misc_feature    6888..7190 
                     /label="SV40 ori"  
     misc_feature    7276..7753 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3957..3963 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3957 
                     /label="Kit"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGAGGCGCT CGCGGCGCCT GGGATCTGCT CTGCGTCCTG TTGGTCCTGC 
     1081 TCCGTGGCCA GACAGCCACG TCTCAGCCAT CTGCAAGTCC AGGGGAGCCG TCTCCGCCAT 
     1141 CCATCCATCC AGCACAATCA GAGTTAATAG TTGAAGCTGG CGACACCCTC AGCCTGACGT 
     1201 GCATTGATCC CGACTTTGTC AGATGGACTT TCAAGACCTA TTTCAATGAA ATGGTTGAGA 
     1261 ATAAAAAAAA TGAATGGATC CAGGAAAAAG CCGAGGCCAC TCGCACGGGC ACATACACGT 
     1321 GCAGCAACAG CAATGGCCTC ACGAGTTCTA TTTACGTGTT TGTTAGAGAT CCTGCCAAAC 
     1381 TTTTCCTGGT TGGCCTTCCC TTGTTTGGCA AAGAAGACAG CGACGCGCTG GTCCGCTGCC 
     1441 CTCTGACAGA CCCACAGGTG TCCAATTATT CCCTCATCGA GTGTGATGGG AAATCTCTCC 
     1501 CCACGGACCT GACGTTTGTC CCAAACCCCA AGGCTGGCAT CACCATCAAA AACGTGAAGC 
     1561 GCGCCTACCA CCGGCTCTGT GTCCGCTGTG CTGCTCAGCG TGACGGTACA TGGCTGCATT 
     1621 CTGACAAATT CACCCTCAAA GTGCGGGCAG CCATCAAGGC TATCCCTGTT GTGTCTGTGC 
     1681 CTGAAACAAG TCACCTCCTT AAGAAAGGGG ACACATTTAC GGTGGTGTGC ACCATAAAAG 
     1741 ATGTGTCTAC ATCCGTGAAC TCCATGTGGC TAAAGATGAA CCCTCAGCCT CAGCACATAG 
     1801 CCCAGGTAAA GCACAATAGC TGGCACCGGG GTGACTTCAA TTATGAACGC CAGGAGACGC 
     1861 TGACTATCAG CTCGGCAAGA GTTGACGATT CTGGAGTGTT CATGTGTTAT GCCAATAATA 
     1921 CTTTTGGATC AGCAAATGTC ACAACAACCT TGAAAGTAGT AGAAAAAGGA TTCATCAACA 
     1981 TCTCCCCTGT GAAGAACACT ACAGTATTTG TAACCGATGG AGAAAACGTA GATTTGGTTG 
     2041 TTGAATACGA GGCCTACCCC AAACCCGAGC ACCAGCAGTG GATATATATG AACAGGACCT 
     2101 CGGCTAACAA AGGGAAGGAT TATGTCAAAT CTGATAACAA AAGCAACATC AGATATGTGA 
     2161 ACCAACTTCG CCTGACCAGA TTAAAAGGCA CAGAAGGAGG CACTTATACC TTTCTGGTGT 
     2221 CCAACTCTGA TGCCAGTGCT TCCGTGACAT TCAACGTTTA CGTGAACACA AAACCAGAAA 
     2281 TCCTGACGTA CGACAGGCTC ATAAATGGCA TGCTCCAGTG TGTGGCAGAG GGATTCCCGG 
     2341 AGCCCACAAT AGATTGGTAT TTTTGTACAG GAGCAGAGCA AAGGTGTACC ACTCCTGTCT 
     2401 CACCAGTGGA CGTACAGGTC CAGAATGTAT CTGTGTCACC ATTTGGAAAA CTGGTGGTTC 
     2461 AGAGTTCCAT AGACTCCAGC GTCTTCCGGC ACAACGGCAC GGTGGAGTGT AAGGCCTCCA 
     2521 ACGATGTGGG CAAGAGTTCC GCCTTCTTTA ACTTTGCATT TAAAGGTAAC AACAAAGAGC 
     2581 AAATCCAGGC CCACACTCTG TTCACGCCGC TGCTCATTGG CTTTGTGGTT GCAGCTGGCG 
     2641 CGATGGGGAT CATTGTGATG GTGCTCACCT ACAAATATTT GCAGAAACCC ATGTATGAAG 
     2701 TACAATGGAA GGTTGTCGAG GAGATAAATG GAAACAATTA TGTTTACATA GACCCGACGC 
     2761 AACTTCCTTA TGATCACAAA TGGGAGTTTC CCAGAAACAG GCTGAGTTTT GGAAAGACAT 
     2821 TGGGAGCTGG TGCCTTCGGG AAGGTCGTTG AGGCCACTGC ATATGGCTTG ATTAAGTCGG 
     2881 ATGCTGCCAT GACAGTTGCC GTGAAGATGC TCAAACCAAG TGCCCATTTA ACAGAAAGAG 
     2941 AGGCCCTAAT GTCGGAACTG AAGGTCCTGA GCTACCTGGG CAATCACATG AATATTGTGA 
     3001 ACCTGCTTGG CGCATGCACG GTGGGAGGGC CCACCCTGGT CATTACAGAA TATTGTTGCT 
     3061 ATGGTGATCT TTTGAATTTT TTGAGAAGGA AGCGTGACTC GTTTATTTTC TCAAAGCAAG 
     3121 AAGAGCAGGC AGAAGCGGCA CTTTATAAGA ACCTTCTGCA CTCAACGGAG CCTTCCTGTG 
     3181 ACAGTTCAAA TGAATATATG GACATGAAGC CTGGCGTTTC CTACGTGGTG CCAACCAAGA 
     3241 CAGACAAGAG GAGATCCGCA AGAATAGACT CGTACATAGA AAGAGACGTG ACTCCTGCCA 
     3301 TCATGGAAGA TGACGAGCTG GCTCTGGACC TGGATGATTT GCTGAGCTTC TCCTACCAGG 
     3361 TGGCCAAGGG CATGGCGTTC CTCGCCTCCA AGAATTGTAT TCACAGAGAT TTGGCAGCCA 
     3421 GGAATATCCT CCTCACTCAC GGGCGGATCA CAAAGATTTG CGATTTCGGG CTAGCCAGAG 
     3481 ACATCAGGAA TGATTCGAAT TACGTGGTCA AAGGAAATGC ACGACTGCCC GTGAAGTGGA 
     3541 TGGCACCAGA GAGCATTTTC AGCTGCGTGT ACACATTTGA AAGTGATGTC TGGTCCTATG 
     3601 GGATTTTCCT CTGGGAGCTC TTCTCCTTAG GAAGCAGCCC CTACCCAGGG ATGCCGGTCG 
     3661 ACTCCAAGTT CTACAAGATG ATCAAGGAAG GCTTCCGGAT GGTCAGCCCG GAGCACGCGC 
     3721 CTGCCGAAAT GTATGACGTC ATGAAGACTT GCTGGGACGC TGACCCCTTG AAAAGGCCAA 
     3781 CATTCAAGCA GGTTGTCCAA CTTATTGAGA AGCAGATCTC GGACAGCACC AAGCACATTT 
     3841 ACTCCAACTT GGCAAACTGC AACCCCAACC CAGAGAACCC CGTGGTGGTG GACCATTCCG 
     3901 TGAGGGTCAA CTCGGTGGGC AGCAGCGCCT CTTCTACGCA GCCCCTGCTC GTGCACGAAG 
     3961 ATGCCACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     4021 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     4081 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     4141 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     4201 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     4261 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     4321 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     4381 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     4441 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     4501 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     4561 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     4621 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     4681 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     4741 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     4801 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     4861 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     4921 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     4981 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     5041 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     5101 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     5161 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     5221 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     5281 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     5341 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     5401 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     5461 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     5521 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     5581 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     5641 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     5701 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     5761 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     5821 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     5881 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     5941 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     6001 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     6061 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     6121 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     6181 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     6241 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     6301 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     6361 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     6421 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     6481 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     6541 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     6601 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     6661 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     6721 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     6781 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     6841 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     6901 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     6961 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     7021 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     7081 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7141 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7201 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     7261 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     7321 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     7381 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     7441 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     7501 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     7561 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     7621 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     7681 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     7741 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     7801 AAATTTAACG CGAATTTT
//