LOCUS       MR227416                8424 bp    DNA     circular  SYN 
DEFINITION  Plcb2 (Myc-DDK-tagged) - Mouse phospholipase C, beta 2 (Plcb2)
ACCESSION   NM_177568
VERSION     NM_177568.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4581..4672 
                     /label="Myc-DDK"  
     misc_feature    4702..5287 
                     /label="PolyA signal"  
     misc_feature    4759..4778 
                     /label="Restriction Enzyme"  
     misc_feature    5563..6235 
                     /label="ColE1"  
     misc_feature    6665..7459 
                     /label="Kan/Neo"  
     misc_feature    7494..7796 
                     /label="SV40 ori"  
     misc_feature    7882..8359 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4563..4569 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4563 
                     /label="Plcb2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCTTTGCTC AACCCTGTTC TATTGCCCCC TAACGTGAAG GCATATCTGA 
     1081 GCCAAGGGGA GCGCTTCATC AAGTGGGACG ATGAAACTTC AATAGCCTCT CCTGTTATCC 
     1141 TCCGTGTGGA TCCCAAGGGC TATTACTTAT ACTGGACATA TCAAAATCAG GAGATGGAGT 
     1201 TCCTGGATGT CACGAGTATC CGAGATACTC GCTTTGGGAA GTTTGCAAAG ATACCCAAGA 
     1261 GCCAGAAACT CCGGGAGGTC TTCAACATGG ACTTTCCAGA CAACCACTTC CTGCTGAAAA 
     1321 CACTCACAGT GGTGTCCGGC CCTGACATGG TGGACCTCAC CTTCTACAAC TTTGTCTCTT 
     1381 ACAAGGAGAA CGTAGGCAAG GACTGGGCTG AGGACGTGCT AGCTCTCGCC AAACACCCGA 
     1441 TGACAGTCAA TGCTCCTCGA AGCACATTCC TGGACAAGAT CCTGGTGAAG CTTAAGATGC 
     1501 AGCTCAACCC TGAAGGGAAG ATTCCCGTGA AGAATTTTTT CCAGATGTTT CCTGCTGATC 
     1561 GAAAACGGGT GGAAGCTGCC CTCGGTGCTT GTCACCTTGC AAAAGGCAAA AATGATGCTA 
     1621 TCAACCCAGA GGACTTCCCA GAATCCGTGT ACAAGAGCTT CCTCATGAGC CTCTGTCCTC 
     1681 GGCCAGAAAT CGACGAGATC TTCACTTCCT ACCACTCTAA AGCTAAGCCC TACATGACCA 
     1741 AGGAACACCT GACCAAATTC ATCAATCAGA AGCAGCGAGA CCCTCGACTC AACTCCTTGC 
     1801 TGTTCCCACC AGCCCGGCCT GAGCAAGTTC AAGTGCTCAT TGACAAGTAC GAACCCAGCG 
     1861 GCATCAATGT GCAGAGGGGC CAGCTGTCAC CAGAGGGGAT GGTCTGGTTT CTCTGTGGAC 
     1921 CAGAGAACAG TGTGCTGGCC CACGATACAC TGCTGATCCA CCAAGACATG ACACAACCAC 
     1981 TGAATCACTA TTTCATCAAC TCCTCACACA ACACCTACCT GACAGCCGGC CAGTTCTCAG 
     2041 GCCTTTCCTC GGCTGAGATG TACCGCCAGG TGCTGCTGTC TGGCTGCCGG TGTGTAGAGT 
     2101 TAGACTGTTG GAAGGGCAAG CCCCCTGACG AAGAGCCCAT TATCACCCAC GGCTTCACCA 
     2161 TGACCACAGA TATCTTGTTC AAGGAAGCAA TTGAAGCCAT TGCAGAAAGT GCCTTTAAGA 
     2221 CCTCCCCGTA TCCTGTCATC CTATCATTTG AAAACCATGT AGACTCACCC CGGCAACAGG 
     2281 CTAAGATGGC TGAGTACTGC AGGAGCATGT TTGGAGAGAC CTTGCTCACA GATCCCCTGG 
     2341 AAAATTTCCC TCTGAAACCT GGCATCCCTC TGCCCAGCCC CGAGGACCTC CGGGGCAAGA 
     2401 TCCTCATTAA GAATAAGAAG AACCAGTTTT CTGGGCCAGC ATCCCCCAGC AAGAAGCCTG 
     2461 GTGGGGTGGC TGAGGGCAGC CTCCCGTCTA GTGTCCCTGT AGAAGAGGAC ACGGGGTGGA 
     2521 CTGCTGAGGA CCGGACTGAG GTGGAGGAGG AGGAGGTGGT GGAAGAGGAG GAAGAGGAGG 
     2581 AGTCAGGAAA CCTGGATGAA GAAGAAATTA AGAAGATGCA GTCGGATGAG GGCACAGCAG 
     2641 GCCTGGAGGT GACAGCTTAT GAGGAAATGT CCAGCCTTGT CAATTATATC CAGCCCACCA 
     2701 AGTTTATCTC CTTCGAGTTC TCTGCACAGA AGAACAGAAG TTACGTTGTC TCTTCCTTCA 
     2761 CGGAGCTCAA GGCCTACGAG CTGCTCTCCA AGGCCTCCAT GCAGTTTGTG GACTATAATA 
     2821 AACGCCAGAT GAGCCGTGTG TACCCCAAGG GCACACGCAT GGACTCTTCC AACTACATGC 
     2881 CCCAGATGTT CTGGAATGCT GGATGTCAGA TGGTTGCCCT CAATTTCCAG ACAATGGACC 
     2941 TGCCCATGCA GCAGAACATG GCACTGTTTG AGTTCAACGG GCAGAGTGGC TACCTCCTAA 
     3001 AGCACGAGTT CATGCGCAGA CTGGACAAGC AGTTCAACCC CTTCTCAGTG GACCGCATTG 
     3061 ATGTGGTGGT AGCCACCACC CTTTCCATTA CGATCATCTC TGGGCAGTTC CTGTCAGAGC 
     3121 GCAGTGTACG CACCTATGTG GAAGTGGAAC TGTTTGGCCT CCCAGGGGAC CCCAAGAGGC 
     3181 GGTATCGAAC CAAGCTGTCA CCTACTGCTA ACTCCATCAA TCCTGTCTGG AAAGAGGAAC 
     3241 CCTTTATCTT TGAGAAGATC TTGATGCCTG AGCTGGCCTC CCTCAGGATA GCTGTGATGG 
     3301 AAGAAGGCAG CAAATTTCTT GGACACCGCA TCATCCCCAT CAATGCCTTA CATTCTGGAT 
     3361 ACCACCATCT GTGCCTGCGC AGTGAGAGCA ACATGGCCCT TACCATGCCA GCTCTCTTTG 
     3421 TCTTCCTGGA GATGAAGGAC TACATACCTG ACACCTGGGC AGATCTTACA GTAGCCCTTG 
     3481 CCAACCCCAT CAAGTACTTC AATGCCCAGG ATAAGAAGTC CGTGAAGCTC AAAGGAGTGA 
     3541 CAGGAAGTCT GCCCGAGAAA CTCTTCTCTG GGACACCTGT TGCCAGTCAG TCCAATGGGG 
     3601 CGCCAGTCTC GGCAGGCAAT GGGTCAACAG CGCCTGGGAC CAAGGCCACG GGGGAAGAAG 
     3661 CTACGAAAGA AGTGACAGAG CCACAGACCG CCAGCTTGGA AGAGTTGCGG GAACTGAAGG 
     3721 GCGTGGTGAA ACTGCAGCGA AGACATGAGA AGGAGCTTCG AGAGTTGGAG CGCCGTGGAG 
     3781 CCCGGCGCTG GGAGGAACTG CTGCAGCGTG GCGCCGCACA GCTGGCGGAG CTCCAGACCC 
     3841 AGGCAGCAGG CTGCAAGCTC CGCCCAGGCA AGGGCTCCCG CAAGAAGAGG ACCCTGCCCT 
     3901 GTGAGGAGAC CGTCGTGGCG CCTAGCGAGC CCCACGACCG GGCGGACCCG CGCGTGCAGG 
     3961 AGCTGAAGGA CAGGCTGGAG CAGGAGCTGC AGCAGCAAGG CGAGGAGCAG TACCGCTCCG 
     4021 TCCTCAAGCG CAAGGAGCAG CACGTGACCG AGCAAATCGC CAAGATGATG GAGCTGGCCA 
     4081 GAGAGAAACA GGCTGCTGAA CTCAAGACCT TCAAGGAGAC CTCAGAAACT GACACCAAAG 
     4141 AAATGAAGAA AAAACTGGAG GCCAAGAGGC TGGAACGGAT CCAAGCCATG ACCAAGGTCA 
     4201 CCACAGACAA GGTGGCCCAG GAGAGGCTCA AGAGAGAAAT TAACAACTCC CACATCCAGG 
     4261 AAGTGGTCCA GGCTGTCAAG CAGATGACAG AGACCCTGGA GCGTCACCAG GAGAAGCTGG 
     4321 AAGAGAGGCA GACAGCCTGC CTGGAGCAGA TCCAGGCAAT GGAAAAGCAG TTCCAGGAGA 
     4381 AGGCGCTGGC AGAGTATGAG GCCAAGATGA AGGGCCTGGA GGCTGAAGTA AAGGAGTCGG 
     4441 TGCGGGCCTA CTTCAAGGAC TGCTTCCCCA CCGAAGCAGA AGACAAGCCT GAGAGGTCCT 
     4501 GTGAGGCCTC TGAGGAGTCG TGTCCACAGG AACCACTTGT GAGCAAGGCA GACACTCAGG 
     4561 AGAGCCGCCT CACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     4621 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     4681 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     4741 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     4801 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     4861 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     4921 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     4981 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     5041 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     5101 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     5161 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     5221 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     5281 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     5341 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     5401 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     5461 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     5521 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     5581 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     5641 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     5701 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     5761 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     5821 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     5881 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     5941 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     6001 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     6061 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     6121 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     6181 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     6241 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     6301 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     6361 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     6421 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     6481 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     6541 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     6601 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     6661 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     6721 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     6781 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     6841 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     6901 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     6961 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     7021 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     7081 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     7141 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     7201 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     7261 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     7321 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     7381 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     7441 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     7501 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     7561 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     7621 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     7681 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7741 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7801 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     7861 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     7921 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     7981 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     8041 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     8101 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     8161 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     8221 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     8281 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     8341 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     8401 TAACAAAAAT TTAACGCGAA TTTT
//