LOCUS       MR227397                8394 bp    DNA     circular  SYN 
DEFINITION  Grm5 (Myc-DDK-tagged) - Mouse glutamate receptor, metabotropic 5 (Grm5), transcript variant a
ACCESSION   NM_001081414
VERSION     NM_001081414.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4551..4642 
                     /label="Myc-DDK"  
     misc_feature    4672..5257 
                     /label="PolyA signal"  
     misc_feature    4729..4748 
                     /label="Restriction Enzyme"  
     misc_feature    5533..6205 
                     /label="ColE1"  
     misc_feature    6635..7429 
                     /label="Kan/Neo"  
     misc_feature    7464..7766 
                     /label="SV40 ori"  
     misc_feature    7852..8329 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4533..4539 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4533 
                     /label="Grm5"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTCCTTCTG TTGATTCTGT CAGTCCTACT TCTGAAAGAA GATGTACGAG 
     1081 GAAGTGCACA GTCCAGTGAG AGGAGGGTGG TGGCTCACAT GCCAGGTGAC ATTATTATTG 
     1141 GAGCTCTCTT CTCGGTCCAC CACCAACCAA CTGTGGACAA AGTTCATGAG AGGAAGTGTG 
     1201 GGGCAGTCCG TGAGCAGTAT GGCATTCAGA GAGTGGAAGC CATGCTGCAT ACCTTGGAAA 
     1261 GGATCAATTC AGATCCCACA CTCTTGCCCA ACATCACACT AGGCTGTGAG ATAAGAGATT 
     1321 CCTGCTGGCA TTCTGCTGTG GCCCTAGAGC AAAGCATTGA GTTTATAAGG GATTCCCTCA 
     1381 TCTCTTCGGA AGAGGAAGAA GGCTTGGTAC GCTGTGTAGA TGGCTCTTCT TCCTTCCGCT 
     1441 CCAAGAAACC CATAGTGGGA GTCATTGGGC CTGGCTCGAG TTCTGTGGCC ATTCAAGTTC 
     1501 AGAACTTGCT CCAGCTTTTC AACATACCTC AGATTGCTTA CTCTGCAACT AGCATGGATT 
     1561 TGAGTGACAA GACTCTATTC AAGTACTTCA TGAGGGTTGT ACCTTCGGAT GCCCAGCAAG 
     1621 CCCGAGCCAT GGTAGACATA GTGAAGAGAT ACAACTGGAC TTATGTCTCA GCTGTGCACA 
     1681 CAGAAGGCAA CTATGGAGAA AGTGGGATGG AGGCTTTCAA AGATATGTCA GCGAAGGAAG 
     1741 GGATTTGCAT CGCCCACTCT TACAAAATCT ACAGCAATGC TGGGGAACAG AGCTTTGACA 
     1801 AGCTGTTGAA AAAGCTCAGA AGTCATTTAC CTAAAGCCCG GGTGGTAGCC TGCTTCTGTG 
     1861 AAGGCATGAC AGTTCGAGGT CTGCTCATGG CCATGAGACG CTTGGGTCTA GCAGGGGAAT 
     1921 TTCTACTTCT GGGCAGTGAT GGCTGGGCTG ACAGGTATGA CGTGACAGAT GGGTATCAGC 
     1981 GAGAAGCTGT CGGTGGGATT ACAATCAAGC TCCAGTCTCC TGATGTCAAG TGGTTTGATG 
     2041 ATTATTATCT GAAGCTCCGG CCAGAAACAA ACCTCAGAAA CCCTTGGTTT CAAGAATTTT 
     2101 GGCAGCATCG TTTTCAGTGC CGGCTAGAAG GGTTTGCACA GGAGAACAGC AAGTACAACA 
     2161 AGACTTGCAA CAGTTCTCTA ACTCTGAGAA CGCATCATGT TCAAGATTCC AAAATGGGAT 
     2221 TTGTGATCAA TGCAATCTAT TCTATGGCTT ATGGGCTCCA CAACATGCAG ATGTCCCTGT 
     2281 GTCCAGGCTA TGCAGGCCTC TGTGATGCAA TGAAGCCAAT TGATGGGCGG AAACTTTTGG 
     2341 ACTCCCTGAT GAAAACCAAC TTTACTGGAG TTTCCGGAGA TATGATTCTA TTTGATGAAA 
     2401 ATGGAGACTC TCCAGGAAGG TATGAAATAA TGAATTTCAA GGAAATGGGA AAAGATTATT 
     2461 TTGATTACAT CAATGTTGGA AGTTGGGACA ATGGGGAATT AAAAATGGAT GATGATGAAG 
     2521 TGTGGTCCAA GAAAAATAAC ATCATCAGAT CTGTGTGCAG TGAACCGTGT GAGAAAGGAC 
     2581 AGATAAAGGT GATCCGGAAG GGAGAAGTCA GCTGTTGTTG GACCTGCACA CCTTGTAAGG 
     2641 AGAATGAGTA TGTTTTTGAT GAGTACACCT GCAAGGCGTG CCAACTGGGG TCCTGGCCCA 
     2701 CTGACGACTT GACAGGTTGT GATTTGATCC CAGTCCAGTA TCTTCGATGG GGTGACCCTG 
     2761 AGCCCATTGC AGCTGTGGTG TTTGCCTGCC TCGGTCTGCT AGCCACCCTC TTCGTTACTG 
     2821 TAATCTTCAT CATTTATCGG GACACTCCAG TGGTCAAGTC TTCCAGCAGG GAACTCTGCT 
     2881 ACATTATCCT TGCTGGCATC TGCCTGGGTT ACTTGTGTAC CTTCTGCCTC ATTGCAAAGC 
     2941 CCAAACAGAT TTACTGCTAC CTTCAGAGAA TTGGCATCGG TCTCTCTCCA GCCATGAGCT 
     3001 ACTCAGCCCT TGTAACGAAG ACCAACCGTA TTGCAAGGAT TCTAGCTGGC AGCAAGAAGA 
     3061 AGATCTGTAC CAAAAAGCCC AGATTCATGA GCGCCTGTGC TCAGTTAGTG ATCGCTTTCA 
     3121 TTCTCATCTG TATACAGTTG GGTATTATTG TGGCCCTCTT TATCATGGAG CCTCCGGATA 
     3181 TAATGCATGA CTATCCAAGC ATCCGAGAAG TCTACTTGAT TTGTAACACC ACCAACCTAG 
     3241 GGGTTGTCAC TCCTCTTGGA TACAATGGAT TATTGATTTT GAGTTGCACA TTCTATGCGT 
     3301 TCAAGACCAG AAATGTTCCA GCCAACTTTA ACGAGGCCAA ATATATTGCT TTCACCATGT 
     3361 ACACAACCTG CATCATATGG CTGGCCTTTG TGCCTATCTA CTTTGGCAGC AACTACAAAA 
     3421 TCATCACCAT GTGTTTCTCA GTCAGCCTCA GTGCCACAGT GGCCCTGGGT TGCATGTTTG 
     3481 TGCCGAAGGT GTACATCATC CTAGCCAAAC CGGAGAGAAA TGTGCGCAGC GCCTTCACAA 
     3541 CCTCTACAGT GGTGCGCATG CACGTAGGAG ATGGCAAGTC ATCATCCGCT GCCAGCAGAT 
     3601 CCAGCAGCCT AGTCAACCTG TGGAAGAGGA GGGGCTCGTC TGGGGAAACC CTAAGCTCCA 
     3661 ATGGAAAATC CGTGACTTGG GCCCAGAATG AGAAGAGCAC CCGGGGGCAG CACCTGTGGC 
     3721 AGCGACTGTC TGTCCATATC AACAAGAAGG AGAACCCCAA CCAGACAGCA GTCATCAAAC 
     3781 CCTTCCCCAA GAGCACAGAG AGCCGCGGGC AGGGTGCAGG GGCAGGTGGT GGCTCTGGCC 
     3841 CCGGTGCAGC TGGTGCTGGT AGCGCAGGAT GCACAGCGAC AGGCGGCCCA GAGCCACCAG 
     3901 ACGCCGGCCC CAAGGCGCTT TATGATGTCG CAGAGGCAGA GGAGCGCTTC CCAGCGGCTG 
     3961 CCAGGCCGCG CTCGCCATCG CCCATCAGTA CGCTGAGCCA CCTGGCAGGC TCGGCGGGCC 
     4021 GCACAGACGA CGACGCGCCG TCGCTGCACT CGGAGACCGC TGCACGCAGC AGCTCATCCC 
     4081 AGGGCTCGCT CATGGAGCAG ATTAGCAGCG TGGTGACGCG CTTCACCGCC AACATCACCG 
     4141 AGCTCAACTC CATGATGTTG TCCACCGCGG CTGCGCCGGG GCCCCCTGGT ACCCCTATCT 
     4201 GCTCTTCCTA CCTGATCCCC AAAGAGATCC AGCTGCCCAC GACCATGACG ACCTTCGCAG 
     4261 AGATCCAGCC TCTGCCGGCC ATCGAGGTGA CCGGAGGAGC TCAGCCGGCG ACAGGGCCAT 
     4321 CACCTGCCCA AGAGACGCCC GCAGGAGCTG AAGCCGCCCC AGGAAAACCG GATCTGGAGG 
     4381 AGCTGGTGGC CCTCACTCCA CCATCGCCCT TCAGGGACTC GGTGGACTCG GGGAGCACCA 
     4441 CCCCAAACTC TCCAGTCTCC GAATCGGCCC TCTGCATCCC ATCCTCTCCC AAATATGACA 
     4501 CTCTCATCAT CAGAGATTAC ACGCAGAGTT CTTCATCGTT GACGCGTACG CGGCCGCTCG 
     4561 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     4621 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     4681 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     4741 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     4801 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     4861 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     4921 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     4981 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     5041 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     5101 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     5161 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     5221 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     5281 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     5341 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     5401 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     5461 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     5521 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     5581 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     5641 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     5701 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     5761 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     5821 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     5881 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     5941 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     6001 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     6061 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     6121 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     6181 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     6241 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     6301 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     6361 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     6421 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     6481 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     6541 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     6601 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     6661 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     6721 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     6781 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     6841 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     6901 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     6961 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     7021 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     7081 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     7141 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     7201 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     7261 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     7321 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     7381 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     7441 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     7501 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     7561 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     7621 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7681 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     7741 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     7801 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     7861 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     7921 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     7981 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     8041 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     8101 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     8161 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     8221 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     8281 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     8341 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//