LOCUS       MR227367                7425 bp    DNA     circular  SYN 
DEFINITION  Nefm (Myc-DDK-tagged) - Mouse neurofilament, medium polypeptide (Nefm)
ACCESSION   NM_008691
VERSION     NM_008691.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3582..3673 
                     /label="Myc-DDK"  
     misc_feature    3703..4288 
                     /label="PolyA signal"  
     misc_feature    3760..3779 
                     /label="Restriction Enzyme"  
     misc_feature    4564..5236 
                     /label="ColE1"  
     misc_feature    5666..6460 
                     /label="Kan/Neo"  
     misc_feature    6495..6797 
                     /label="SV40 ori"  
     misc_feature    6883..7360 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3564..3570 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3564 
                     /label="Nefm"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGCTACACG CTGGACTCGC TGGGCAACCC GTCCGCCTAC CGGCGGGTCA 
     1081 CCGAGACCCG CTCCAGCTTC AGCCGCGTGA GCGGTTCCCC GTCCAGCGGC TTCCGCTCGC 
     1141 AGTCCTGGTC CCGCGGCTCG CCCAGCACCG TGTCCTCCTC CTACAAGCGC AGCGCGCTCG 
     1201 CCCCGCGTCT CGCCTACAGC TCGGCTATGC TCAGCTCGGC CGAGAGCAGC CTCGACTTCA 
     1261 GCCAGTCCTC GTCGCTGCTC AACGGCGGCT CCGGCGGCGA CTACAAACTG TCCCGCTCTA 
     1321 ACGAGAAAGA GCAGCTGCAG GGGCTGAACG ACCGCTTCGC CGGCTACATC GAGAAAGTGC 
     1381 ACTACTTGGA ACAACAGAAC AAGGAGATCG AAGCAGAGAT CCAGGCACTG CGGCAGAAGC 
     1441 AGGCCTCGCA CGCCCAGCTG GGTGATGCTT ACGACCAGGA GATCCGAGAG CTGCGCGCCA 
     1501 CCCTCGAGAT GGTGAACCAC GAGAAGGCTC AAGTGCAGCT GGACTCCGAT CACTTGGAGG 
     1561 AAGACATCCA CCGGCTCAAG GAGCGCTTCG AGGAGGAGGC GCGGCTGCGG GACGACACCG 
     1621 AGGCTGCCAT TCGCGCGCTG CGCAAAGACA TCGAAGAGTC GTCGATGGTT AAGGTGGAGC 
     1681 TGGACAAGAA GGTGCAGTCG CTGCAGGATG AGGTGGCTTT CCTGCGGAGC AATCACGAAG 
     1741 AGGAGGTGGC CGACCTGCTG GCTCAGATCC AGGCGTCGCA CATCACGGTA GAGCGCAAAG 
     1801 ATTACCTGAA GACAGACATC TCCACGGCGC TGAAGGAGAT CCGCTCCCAG CTCGAGTGTC 
     1861 ACTCAGACCA GAACATGCAC CAGGCCGAAG AGTGGTTCAA ATGCCGCTAC GCCAAGCTCA 
     1921 CCGAGGCGGC CGAGCAGAAC AAGGAGGCCA TTCGCTCTGC CAAGGAAGAG ATCGCCGAGT 
     1981 ACCGGCGCCA GCTGCAGTCC AAGAGCATCG AGCTCGAGTC GGTGCGAGGC ACTAAGGAGT 
     2041 CCCTGGAGCG GCAGCTCAGC GACATCGAGG AGCGCCACAA CCACGACCTC AGCAGCTACC 
     2101 AGGACACCAT CCAGCAGTTG GAAAATGAAC TTCGGGGAAC CAAGTGGGAA ATGGCTCGTC 
     2161 ATTTGCGAGA ATACCAGGAT CTCCTTAACG TCAAGATGGC CCTGGACATC GAGATCGCCG 
     2221 CGTACAGGAA ACTCCTAGAG GGGGAAGAGA CCAGATTTAG CACATTTTCA GGAAGCATCA 
     2281 CCGGGCCTCT GTACACACAC CGACAGCCCT CAGTCACAAT ATCCAGTAAG ATTCAGAAGA 
     2341 CCAAAGTCGA GGCCCCCAAG CTCAAGGTCC AACACAAATT TGTGGAGGAG ATCATCGAAG 
     2401 AAACTAAAGT GGAAGATGAG AAGTCAGAAA TGGAAGAAAC CCTCACAGCC ATCGCAGAGG 
     2461 AGTTGGCAGC CTCCGCCAAA GAGGAGAAGG AAGAGGCCGA AGAAAAGGAG GAGGAACCAG 
     2521 AAGCCGAAAA GTCTCCCGTG AAGTCTCCTG AGGCTAAGGA AGAGGAGGAG GAAGGGGAAA 
     2581 AGGAGGAAGA AGAGGAAGGC CAGGAGGAAG AAGAGGAGGA AGATGAAGGT GTCAAGTCAG 
     2641 ACCAGGCAGA AGAGGGGGGA TCTGAGAAGG AAGGCTCCAG TGAAAAAGAT GAAGGTGAGC 
     2701 AGGAAGAAGA AGAAGGAGAA ACCGAGGCAG AAGGTGAAGG AGAGGAAGCA GAAGCTAAGG 
     2761 AGGAAAAGAA AATTGAGGGA AAGGTTGAGG AAGTGGCTGT CAAGGAGGAA ATCAAGGTCG 
     2821 AGAAGCCTGA GAAAGCCAAA TCCCCTATGC CCAAATCACC CGTGGAAGAA GTAAAGCCAA 
     2881 AACCAGAGGC CAAGGCCGGG AAGGGTGAGC ACAAGGAGGA AGAGAAAGTT GAGGAAGAGA 
     2941 AGAAGGAAGT CACCAAAGAA TCACCCAAGG AAGAGAAGGT GGAGAAAAAG GAGGAGAAGC 
     3001 CAAAAGATGT TGCAGATAAA AAGAAGGCCG AGTCCCCGGT GAAAGAGAAG GCTGTGGAGG 
     3061 AGGTGATCAC CATCAGCAAG TCGGTAAAGG TGAGCCTAGA GAAAGACACC AAAGAGGAGA 
     3121 AGCTGCAGCC GCAGGAGAAG GTGAAGGAGA AGGCAGAGGA GGAGGGGGGC AGTGAGGAGG 
     3181 AAGGGAGTGA CCGTAGCCCG CAGGAGTCCA AGAAGGAAGA CATAGCTATC AATGGGGAGG 
     3241 TGGAAGGAAA AGAGGAGGAG GAGCAGGAAA CTCAGGAGAA GGGCAGTGGG CGGGAGGAGG 
     3301 AGAAAGGGGT GGTCACTAAT GGCTTAGATG TGAGCCCTGC AGAGGAGAAG AAAGGAGAGG 
     3361 ATAGCAGTGA TGATAAAGTG GTGGTCACCA AGAAGGTAGA AAAGATCACC AGCGAGGGGG 
     3421 GCGATGGTGC TACCAAATAC ATCACCAAAT CTGTAACCGT CACTCAAAAG GTTGAAGAGC 
     3481 ATGAGGAGAC CTTTGAGGAG AAGCTGGTCT CAACTAAAAA GGTAGAAAAG GTCACTTCAC 
     3541 ACGCCATAGT CAAGGAAGTC ACCCAGGGTG ACACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     3601 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     3661 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     3721 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     3781 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     3841 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     3901 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     3961 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     4021 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     4081 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     4141 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     4201 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     4261 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     4321 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     4381 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     4441 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     4501 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     4561 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     4621 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     4681 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     4741 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     4801 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     4861 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     4921 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     4981 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     5041 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     5101 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     5161 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     5221 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     5281 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     5341 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     5401 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     5461 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     5521 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     5581 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     5641 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     5701 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     5761 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     5821 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     5881 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     5941 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     6001 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     6061 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     6121 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     6181 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     6241 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     6301 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     6361 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     6421 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     6481 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     6541 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     6601 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     6661 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     6721 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     6781 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     6841 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     6901 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     6961 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     7021 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     7081 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     7141 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     7201 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     7261 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     7321 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     7381 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//