LOCUS       MR227356                8232 bp    DNA     circular  SYN 
DEFINITION  Gucy2d (Myc-DDK-tagged) - Mouse guanylate cyclase 2d (Gucy2d)
ACCESSION   NM_001130693
VERSION     NM_001130693.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4389..4480 
                     /label="Myc-DDK"  
     misc_feature    4510..5095 
                     /label="PolyA signal"  
     misc_feature    4567..4586 
                     /label="Restriction Enzyme"  
     misc_feature    5371..6043 
                     /label="ColE1"  
     misc_feature    6473..7267 
                     /label="Kan/Neo"  
     misc_feature    7302..7604 
                     /label="SV40 ori"  
     misc_feature    7690..8167 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4371..4377 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4371 
                     /label="Gucy2d"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCAGGTCTG CAGCAGGGCT GTCACTTTGA AGGCCAGAAC TGGACAGCAC 
     1081 CACACTGGAA GACCTGCCTG CCCTGCCAGG GCCCGTGGAG ACTCACTGTC AGTCATCTGA 
     1141 AGACTGTCTC TTCCATATCA GTCCTCTCTG TGGTATTTTG GAGTGTCCTA CTTTGGGCTG 
     1201 ACTCCCTTTC CCTGTTAGCA TGGGCAAGAG AGACCTTCAC CCTTGGGGTG CTGGGTCCCT 
     1261 GGGACTGTGA CCCCATCTTT GCCCAGGCCC TCCCCAGCAT AGCTACCCAG CTGGCTGTAG 
     1321 ATCAAGTTAA TCAGGATGCC TCACTGCTGC CAGGCTCACA GTTGGATTTC AAGGTCCTCC 
     1381 CCACAGGCTG TGACACTCCT CATGCCCTGG CCACATTCGT GGCCCACAAG AACATAGTGG 
     1441 CTGCTTTTGT AGGCCCTGTT AATCCTGGGT TCTGCTCAGC AGCAGCCCTG CTGGCCCAAG 
     1501 GCTGGGGCAA GTCCCTCTTC TCCTGGGCCT GTGAAGCTCC AGAGGGAGGA GGTGACTTAG 
     1561 TGCCCACTTT ACCTTCTGCG GCCGATGTGC TACTGTCTGT CATGAGACAC TTTGGCTGGG 
     1621 CTCGCTGGGC CATTGTGTCC TCTCACCAGG ACATCTGGGT AACCACAGCC CAGCAGCTGG 
     1681 CCACTGCTTT CAGGACTCAT GGGCTGCCCA TCGGACTGGT GACCTCTTTG GGACCTGGAG 
     1741 AGAAGGGGGC CACAGAGGTT TGCAAGCAGC TCCACAGTGT TCATGGTCTG AAAATTGTGG 
     1801 TTCTGTGCAT GCACTCGGCG CTGCTTGGAG GCCTGGAGCA GACCACCCTG CTGCACTGTG 
     1861 CCTGGGAGGA GGGCCTCACA GACGGAAGGC TGGTCTTCCT GCCCTACGAC ACGCTGCTCT 
     1921 TTGCCCTGCC CTATGGAAAC CGCTCCTACC TGGTCCTGGA TGACCATGGG CCCCTTCAGG 
     1981 AAGCCTATGA CGCAGTACTC ACTGTCAGTC TGGAGTCCAG TCCTGAGAGC CACGCCTTCA 
     2041 CTGCTACCGA GATGAGTGGA GGGGCAACTG CCAACTTGGA GCCAGAGCAG GTGTCCCCAC 
     2101 TCTTTGGAAC TATCTATGAT GCCGTCATCC TGTTGGCCCA TGCCCTGAAC CGATCTGAGA 
     2161 CCCATGGAGC GGGGCTCTCA GGGGCTCACT TAGGGGACCA CGTAAGAGCT CTTGATGTGG 
     2221 CTGGTTTTAG CCAGAGAATC CGAACAGATG GGAAAGGCAG AAGGCTAGCC CAATATGTCA 
     2281 TCCTGGACAC AGATGGTGAA GGAAGCCAGT TGGTTCCTAC CCACATTCTG GACACAAGCA 
     2341 CATGGCAGGT GCAGCCTCTG GGAAAGCCTA TACACTTTCC TGGGGGGAGC CCTCCAGCCC 
     2401 ATGACGCCAG CTGCTGGTTC GACCCCAACA CACTATGCAT CAGAGGTGTG CAGCCCCTGG 
     2461 GCAGCCTCCT GACTCTGACG ATAGCCTGTG TCCTGGCACT GGTTGGTGGA TTCCTTGCTT 
     2521 ACTTCATCAG GTTAGGCCTC CAGCAGCTGC GGCTGTTGCG GGGCCCCCAT CGGATCCTGC 
     2581 TGACATCTCA GGAGCTCACC TTCCTCCAGC GGACCCCCAG CCGGAGGAGG CCACATGTGG 
     2641 ACAGCGGCAG TGAGTCAAGA AGTGTGGTGG ATGGTGGAAG TCCACGGTCG GTGACCCAGG 
     2701 GGTCAGCAAG GAGCCTACCA GCCTTCCTGG AGCACACCAA CGTGGCCCTG TACCAGGGAG 
     2761 AGTGGGTATG GCTGAAGAAG TTTGAAGCAG GCGTGGCTCC TGATCTTCGG CCAAGTAGCC 
     2821 TTAGCTTCCT GAGAAAGTTG CGGGAGATGC GGCATGAGAA TGTTACTGCC TTCCTGGGCC 
     2881 TCTTTGTGGG CCCTGGGGTC AGTGCAATGG TGCTGGAGCA CTGTGCCCGT GGCAGCCTGG 
     2941 AGGACCTGCT GCAGAATGAG AACCTGAGGC TAGACTGGAC CTTCAAGGCC TCCTTACTGT 
     3001 TGGATCTGAT CCGAGGTTTG CGGTATCTGC ACCATCGGCG CTTCCCCCAC GGGCGCCTCA 
     3061 AGTCCAGGAA CTGTGTGGTG GACACTCGCT TTGTGCTCAA GATCACTGAT CATGGCTATG 
     3121 CAGAGTTCCT GGAGTCTCAC TGTTCTTCCA GGCCCCAGCC AGCCCCAGAA GAGCTTCTGT 
     3181 GGACGGCTCC TGAGCTGCTG CGGGGGCCTG GGAAGGCCAC CTTCAAAGGT GATGTTTTCA 
     3241 GCTTGGCCAT CATCCTGCAG GAGGTACTGA CCCGAGACCC ACCCTACTGC TCCTGGGGAC 
     3301 TCTCAGCAGA AGAGATCATC CGGAAGGTGG CATCTCCCCC TCCTCTGTGC CGGCCACTGG 
     3361 TGTCCCCTGA CCAGGGTCCC CTCGAGTGTA TCCAGTTGAT GCAGCTATGC TGGGAGGAAG 
     3421 CTCCAGATGA CAGGCCAAGC TTGGACCAGA TCTACACACA GTTCAAAAGC ATCAACCAAG 
     3481 GCAAGAAGAC AAGTGTTGTA GACTCCATGC TGCGGATGCT GGAGAAGTAT TCCGAAAGCC 
     3541 TGGAAGATCT GGTCCAGGAG CGGACTGAGG AGCTGGAACT GGAGAGGCGG AAGACAGAGA 
     3601 GGCTGCTCTC GCAGATGCTC CCTCCGTCTG TCGCCCATGC TCTAAAGATG GGGACAACAG 
     3661 TGGAGCCCGA GTACTTCGAC CAGGTCACCA TCTATTTCAG TGACATCGTG GGCTTCACCA 
     3721 CCATCTCAGC CTTGAGTGAG CCCATTGAGG TGGTGGGCTT CCTCAATGAT CTCTACACGC 
     3781 TGTTTGATGC TGTTCTCGAC AGCCATGATG TGTATAAGGT GGAGACCATA GGGGATGCCT 
     3841 ACATGGTGGC TTCTGGTCTC CCTCGGCGCA ATGGAAATCG GCACGCTGCT GAGATCGCCA 
     3901 ACCTGGCCCT GGACATCCTT AGCTATGCAG GCAACTTTCG GATGAGGCAT GCACCCGATG 
     3961 TACCCATCCG TGTCAGGGCA GGTCTGCATT CAGGACCCTG TGTGGCAGGT GTTGTGGGTC 
     4021 TCACCATGCC TCGGTACTGC CTCTTTGGGG ACACCGTCAA CACTGCCTCT AGGATGGAGT 
     4081 CCACTGGGCT GCCTTACAGG ATCCATGTCA GTCAAAGCAC TGTCCAGGCC CTGCTCAGCC 
     4141 TTGATGAAGG CTACAAAATT GATGTCAGAG GTCAGACTGA ACTGAAGGGG AAGGGCTTGG 
     4201 AGGAGACCTA CTGGCTGACA GGGAAGGTAG GATTCTGCAG ACCCCTGCCT ACACCTCTGT 
     4261 CTATCAAGCC TGGAGACCCA TGGCAGGACC GCATAAACCA AGAAATCCGG ACTGGCTTTG 
     4321 CCAAAGCACG CCAGGGACTG GCTGAGCCCA GAAAATCAGG GGAGGCTGGG CCAGGTCCCA 
     4381 CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA 
     4441 TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     4501 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     4561 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     4621 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     4681 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     4741 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     4801 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     4861 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     4921 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     4981 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     5041 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     5101 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCGGCGAGC 
     5161 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 
     5221 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 
     5281 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 
     5341 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 
     5401 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 
     5461 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
     5521 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 
     5581 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 
     5641 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 
     5701 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 
     5761 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
     5821 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 
     5881 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 
     5941 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 
     6001 TTTTAAATCA ATCTAAAGTA TATATGAGTA ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC 
     6061 GACGTTGGCT GCGAGCCCTG GGCCTTCACC CGAACTTGGG GGGTGGGGTG GGGAAAAGGA 
     6121 AGAAACGCGG GCGTATTGGC CCCAATGGGG TCTCGGTGGG GTATCGACAG AGTGCCAGCC 
     6181 CTGGGACCGA ACCCCGCGTT TATGAACAAA CGACCCAACA CCGTGCGTTT TATTCTGTCT 
     6241 TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC TCCTTCCGTG TTTCAGTTAG 
     6301 CCTCCCCCTA GGGTGGGCGA AGAACTCCAG CATGAGATCC CCGCGCTGGA GGATCATCCA 
     6361 GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA TAGAAGGCGG CGGTGGAATC 
     6421 GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAACC CCAGAGTCCC 
     6481 GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG 
     6541 ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA 
     6601 CGGGTAGCCA ACGCTATGTC CTGATAGCGA TCCGCCACAC CCAGCCGGCC ACAGTCGATG 
     6661 AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC 
     6721 ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC 
     6781 GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA 
     6841 GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA 
     6901 AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG 
     6961 TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT 
     7021 TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC 
     7081 CGCGCTGCCT CGTCTTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA 
     7141 ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT 
     7201 TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT 
     7261 CCATCTTGTT CAATCATGCG AAACGATCCT CATCCTGTCT CTTGATCGAT CTTTGCAAAA 
     7321 GCCTAGGCCT CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC 
     7381 CTCGGCCTCT GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT GGGCGGAACT 
     7441 GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG CTGACTAATT 
     7501 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCTGGT 
     7561 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7621 TCCACACCCT AACTGACACA CATTCCACAG CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT 
     7681 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 
     7741 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
     7801 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 
     7861 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC 
     7921 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT 
     7981 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG 
     8041 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT 
     8101 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA 
     8161 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT 
     8221 AACGCGAATT TT
//