LOCUS       MR227349                8364 bp    DNA     circular  SYN 
DEFINITION  Hipk2 (Myc-DDK-tagged) - Mouse homeodomain interacting protein kinase 2 (Hipk2), transcript variant 1
ACCESSION   NM_001136065
VERSION     NM_001136065.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4521..4612 
                     /label="Myc-DDK"  
     misc_feature    4642..5227 
                     /label="PolyA signal"  
     misc_feature    4699..4718 
                     /label="Restriction Enzyme"  
     misc_feature    5503..6175 
                     /label="ColE1"  
     misc_feature    6605..7399 
                     /label="Kan/Neo"  
     misc_feature    7434..7736 
                     /label="SV40 ori"  
     misc_feature    7822..8299 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4503..4509 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4503 
                     /label="Hipk2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCTCACAT GTGCAAGTTT TCTCCCCTCA CACCCTTCAA TCAAGTGCCT 
     1081 TCTGTAGTGT GAAGAAACTA AAAGTAGAGC CAAGTTCCAA CTGGGACATG ACTGGGTACG 
     1141 GCTCCCACAG CAAAGTGTAC AGCCAGAGCA AGAACATACC ACCTTCTCAG CCAGCCTCCA 
     1201 CAACCGTCAG CACCTCCTTG CCAATCCCCA ACCCAAGCCT ACCTTACGAG CAGACCATCA 
     1261 TCTTCCCAGG AAGTACTGGA CACATAGTTG TAACATCAGC AAGTAGCACT TCTGTCACCG 
     1321 GGCAAGTCCT CGGTGGACCA CATAACCTAA TGCGTCGAAG CACTGTGAGC CTCCTTGACA 
     1381 CCTACCAAAA ATGTGGACTC AAGCGCAAGA GTGAGGAGAT TGAGAACACA AGCAGCGTGC 
     1441 AGATCATTGA AGAGCATCCA CCCATGATTC AGAATAATGC CAGCGGGGCC ACTGTAGCCA 
     1501 CTGCCACCAC ATCCACTGCC ACCTCCAAAA ACAGTGGCTC CAACAGTGAA GGGGACTATC 
     1561 AGCTGGTGCA GCATGAAGTA CTGTGCTCCA TGACCAACAC CTATGAGGTT TTAGAGTTCT 
     1621 TGGGGCGAGG GACATTTGGG CAAGTGGTCA AGTGCTGGAA ACGGGGCACC AATGAAATTG 
     1681 TGGCCATTAA GATCCTCAAG AACCACCCCT CCTATGCCCG GCAAGGCCAG ATTGAAGTGA 
     1741 GCATCTTGGC CCGGCTGAGC ACGGAGAGCG CTGATGACTA CAATTTTGTG CGGGCCTATG 
     1801 AGTGCTTCCA GCACAAGAAC CACACGTGCT TAGTCTTTGA GATGTTGGAG CAGAACCTTT 
     1861 ATGACTTTCT GAAACAGAAC AAGTTTAGTC CCTTGCCCCT CAAGTATATT CGCCCAGTCC 
     1921 TCCAGCAGGT AGCCACAGCC CTGATGAAAC TCAAAAGCCT GGGTCTTATC CATGCTGACC 
     1981 TCAAACCAGA AAACATCATG CTGGTGGATC CATCCAGACA ACCGTACCGA GTGAAGGTCA 
     2041 TTGACTTTGG TTCAGCTAGT CATGTGTCCA AAGCTGTCTG CTCTACGTAC TTGCAATCCA 
     2101 GATACTACCG GGCCCCTGAG ATCATCCTTG GTTTGCCATT CTGTGAGGCA ATTGACATGT 
     2161 GGTCCCTTGG TTGTGTCATT GCTGAACTGT TCCTGGGCTG GCCATTATAC CCAGGTGCTT 
     2221 CTGAGTATGA TCAGATTCGG TATATTTCAC AAACACAGGG TTTACCAGCT GAATATTTAT 
     2281 TAAGTGCAGG GACAAAGACA ACTAGGTTTT TCAACCGTGA CACAGACTCA CCGTATCCTT 
     2341 TGTGGAGGCT AAAGACACCA GATGACCATG AAGCAGAGAC GGGAATTAAG TCAAAGGAAG 
     2401 CAAGAAAGTA CATTTTCAAC TGTTTGGATG ATATGGCCCA GGTGAACATG ACAACAGATT 
     2461 TGGAAGGGAG TGACATGTTA GTAGAGAAGG CAGACCGACG GGAGTTCATT GACCTGTTAA 
     2521 AGAAGATGCT GACCATCGAT GCTGATAAGA GAGTCACTCC CATTGAAACT CTGAACCACC 
     2581 CCTTTGTCAC CATGACACAC CTGCTTGACT TCCCCCATAG TGCCCATGTC AAATCTTGTT 
     2641 TCCAAAACAT GGAGATTTGC AAGCGCCGGG TGAATATGTA TGACACAGTG AACCAGAGCA 
     2701 AAACACCTTT CATCACTCAC GTGGCCCCCA GCACATCCAC CAACTTGACC ATGACCTTTA 
     2761 ACAACCAGCT GACCACTGTC CACAACCAGC CCTCAGCGGC ATCCATGGCC GCGGTGGCCC 
     2821 CGCGGAGCAT GCCCCTGCAG ACGGGAACAG CCCAAATTTG TGCCCGACCT GATCCCTTCC 
     2881 AGCAAGCTCT CATCGTGTGT CCCCCTGGCT TCCAAGGCCT GCAGGCCTCT CCCTCCAAGC 
     2941 ACGCTGGCTA CTCAGTGCGG ATGGAAAATG CTGTCCCCAT CGTCACCCAG GCGCCAGGAG 
     3001 CTCAGCCTCT TCAGATCCAA CCAGGCCTGC TTGCTCAGGC CTGGCCAGGT GGGGCCCAAC 
     3061 AGATCCTACT TCCTCCAGCA TGGCAGCAGC TGACTGGCGT GGCCACCCAC ACATCTGTAC 
     3121 AGCATGCAGC TGTGATTCCT GAGACCATGG CAGGCACTCA GCAACTGGCT GACTGGAGGA 
     3181 ACACGCATGC TCATGGAAGT CATTACAATC CCATCATGCA GCAGCCTGCA CTCTTGACTG 
     3241 GTCATGTGAC CCTTCCAGCT GCCCAGCCCT TAAATGTGGG TGTAGCCCAT GTGATGAGGC 
     3301 AACAACCAAC CAGCACCACC TCCTCCAGGA AGAGTAAGCA GCACCAGTCA TCTGTGAGAA 
     3361 ATGTCTCCAC CTGTGAGGTG ACCTCTTCAC AGGCTATCAG CTCCCCTCAG CGATCCAAGC 
     3421 GTGTCAAGGA GAACACTCCC CCACGGTGCG CCATGGTACA CAGCAGCCCA GCTTGCAGCA 
     3481 CCTCAGTCAC CTGTGGGTGG GGCGACGTGG CCTCCAGCAC CACCCGGGAG CGACAGCGGC 
     3541 AGACGATTGT CATCCCCGAC ACCCCGAGCC CCACAGTCAG TGTCATCACC ATCAGCAGTG 
     3601 ACACCGATGA AGAAGAGGAG CAGAAGCACG CCCCCACCAG CACAGTCTCC AAGCAAAGAA 
     3661 AAAATGTCAT CAGCTGTGTC ACCGTCCACG ACTCTCCCTA CTCTGACTCC TCCAGCAACA 
     3721 CCAGCCCCTA CTCGGTGCAG CAGCGCACAG GGCACAACGG CACCAACACC TTGGACACCA 
     3781 AGGGGGGCCT AGAGAATCAC TGCACTGGCA ACCCCCGCAC CATCATCGTA CCCCCACTGA 
     3841 AGACCCAGGC CAGTGAAGTG TTGGTAGAAT GTGACAGCCT AGGGCCAGCG ATCAGTGCCA 
     3901 GTCACCATTC GTCCTCCTTC AAGTCCAAGT CCTCCAGCAC CGTGACCTCC ACCAGTGGAC 
     3961 ACTCTTCAGG GAGCTCTTCA GGAGCCATCG CCTACCGTCA GCAGCGGCCA GGCCCCCACT 
     4021 TCCAGCAGCA GCAGCCCCTC AATCTCAGCC AGGCCCAGCA GCACATGGCT GCGGACCGCA 
     4081 CCGGGAGTCA CCGTCGACAG CAGGCCTACA TCACTCCGAC CATGGCGCAA GCTCCGTACA 
     4141 CCTTCCCGCA CAACAGCCCC AGCCATGGCA CTGTTCACCC CCACCTGGCT GCGGCTGCCC 
     4201 ACCTCCCCAC CCAGCCTCAC CTCTACACCT ATACAGCGCC CACAGCCCTA GGCTCCACCG 
     4261 GCACTGTAGC CCACCTGGTG GCATCCCAAG GCTCAGCACG CCACACCGTG CAGCACACTG 
     4321 CCTATCCGGC CAGCATCGTC CACCAGGTCC CTGTGAGCAT GGGGCCCCGG GTCCTGCCCT 
     4381 CGCCCACCAT CCACCCCAGT CAGTATCCAG CCCAGTTTGC CCACCAGACC TATATCAGCG 
     4441 CCTCGCCAGC CTCCACCGTC TACACTGGAT ACCCACTGAG TCCTGCCAAG GTCAACCAAT 
     4501 ACCCTTACAT AACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     4561 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     4621 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     4681 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     4741 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     4801 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     4861 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     4921 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     4981 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     5041 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     5101 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     5161 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     5221 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     5281 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     5341 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     5401 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     5461 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     5521 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     5581 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     5641 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     5701 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     5761 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     5821 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     5881 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     5941 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     6001 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     6061 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     6121 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     6181 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     6241 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     6301 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     6361 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     6421 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     6481 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     6541 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     6601 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     6661 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     6721 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     6781 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     6841 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     6901 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     6961 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     7021 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     7081 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     7141 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     7201 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     7261 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     7321 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     7381 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     7441 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     7501 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     7561 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     7621 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7681 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7741 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     7801 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     7861 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     7921 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     7981 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     8041 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     8101 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     8161 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     8221 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     8281 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     8341 TAACAAAAAT TTAACGCGAA TTTT
//