LOCUS       MR227348                7242 bp    DNA     circular  SYN 
DEFINITION  Itgb3 (Myc-DDK-tagged) - Mouse integrin beta 3 (Itgb3)
ACCESSION   NM_016780
VERSION     NM_016780.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3399..3490 
                     /label="Myc-DDK"  
     misc_feature    3520..4105 
                     /label="PolyA signal"  
     misc_feature    3577..3596 
                     /label="Restriction Enzyme"  
     misc_feature    4381..5053 
                     /label="ColE1"  
     misc_feature    5483..6277 
                     /label="Kan/Neo"  
     misc_feature    6312..6614 
                     /label="SV40 ori"  
     misc_feature    6700..7177 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3381..3387 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3381 
                     /label="Itgb3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCGAGCGCAG TGGCCGGGAC AACTCTGGGC CGCTCTGCTG GCGCTGGGGG 
     1081 CGCTGGCGGG CGTTGTTGTT GGAGAGTCCA ACATCTGTAC CACACGAGGC GTGAACTCCT 
     1141 GCCAGCAGTG TCTGGCTGTG AGTCCTGTGT GTGCCTGGTG CTCAGATGAG ACTTTGTCTC 
     1201 AGGGCTCACC CCGATGTAAC CTGAAGGAGA ACCTGCTGAA GGACAATTGT GCTCCAGAGT 
     1261 CTATTGAGTT CCCAGTCAGT GAGGCCCAGA TCCTGGAGGC TAGGCCACTC AGCAGCAAGG 
     1321 GCTCTGGAAG CAGCGCCCAG ATCACTCAAG TCAGCCCTCA GAGGATTGCC CTTCGACTAC 
     1381 GGCCAGATGA TTCGAAGATC TTCTCACTTC AAGTGCGGCA GGTGGAGGAT TACCCCGTGG 
     1441 ACATCTACTA CTTGATGGAC CTGTCTTTCT CCATGAAGGA TGATCTGTCC AGCATCCAGA 
     1501 CCCTGGGTAC CAAGTTGGCC TCTCAGATGC GCAAGCTTAC TAGCAACCTT CGGATTGGCT 
     1561 TTGGGGCCTT CGTGGACAAG CCTGTATCGC CGTACATGTA CATCTCCCCA CCACAGGCAA 
     1621 TCAAAAACCC CTGTTACAAT ATGAAGAATG CCTGCTTGCC CATGTTTGGC TACAAACACG 
     1681 TGCTGACGCT AACCGACCAG GTGTCCCGCT TCAATGAAGA AGTGAAGAAA CAGAGCGTGT 
     1741 CCCGTAATCG AGATGCCCCA GAGGGCGGCT TTGACGCCAT CATGCAGGCT ACAGTATGTG 
     1801 ATGAAAAAAT TGGCTGGAGG AATGACGCAT CCCATTTGCT AGTGTTTACC ACGGATGCCA 
     1861 AGACCCATAT TGCCCTGGAT GGAAGGCTGG CAGGCATTGT CCTGCCCAAT GATGGGCACT 
     1921 GTCACATTGG CACCGACAAC CACTACTCTG CCTCCACTAC CATGGACTAC CCATCTCTGG 
     1981 GGCTGATGAC TGAGAAACTA TCCCAGAAAA ACATTAACTT GATCTTTGCA GTGACTGAAA 
     2041 ATGTCGTCAG CCTTTACCAG AATTATAGTG AGCTCATTCC TGGGACCACA GTGGGAGTCC 
     2101 TGTCTGATGA CTCAAGCAAC GTCCTCCAGC TCATTGTTGA TGCTTACGGG AAAATCCGCT 
     2161 CTAAAGTGGA GCTGGAAGTA CGTGACCTGC CGGAAGAACT GTCACTGTCC TTCAATGCCA 
     2221 CCTGCCTCAA CAACGAGGTT ATCCCGGGCC TCAAGTCTTG TGTGGGCCTC AAGATTGGAG 
     2281 ACACGGTGAG CTTTAGTATC GAGGCCAAGG TGCGTGGCTG CCCCCAGGAG AAGGAGCAGT 
     2341 CTTTCACTAT CAAGCCTGTG GGCTTTAAGG ACAGCCTCAC CGTCCAGGTG ACCTTCGACT 
     2401 GTGACTGTGC CTGCCAGGCC TTTGCCCAGC CTTCCAGCCC ACGCTGCAAC AATGGGAACG 
     2461 GGACTTTTGA GTGTGGGGTG TGCCGCTGTG ACCAGGGCTG GCTGGGGTCC ATGTGTGAGT 
     2521 GCTCTGAGGA GGATTACCGA CCCTCTCAGC AGGAAGAGTG CAGCCCCAAG GAGGGCCAGC 
     2581 CCATCTGCAG CCAGCGGGGA GAGTGCCTCT GTGGCCAGTG TGTCTGCCAT AGCAGCGACT 
     2641 TCGGCAAGAT CACTGGCAAG TACTGTGAGT GCGATGACTT CTCCTGCGTC CGCTACAAAG 
     2701 GGGAGATGTG TTCCGGCCAT GGGCAATGTA ACTGTGGGGA CTGCGTGTGT GACTCGGACT 
     2761 GGACTGGCTA CTACTGCAAC TGTACTACAC GCACTGACAC CTGCATGTCC ACCAATGGGC 
     2821 TGCTGTGCAG CGGCCGGGGC AACTGCGAGT GCGGCAGCTG TGTGTGCGTC CAGCCAGGCT 
     2881 CCTATGGAGA CACCTGTGAG AAGTGCCCCA CCTGCCCAGA TGCCTGCTCC TTTAAGAAGG 
     2941 AGTGTGTGGA GTGTAAGAAG TTCAACCGGG GAACGCTCCA TGAAGAAAAC ACCTGCAGCC 
     3001 GCTACTGCCG GGATGACATC GAGCAGGTGA AAGAGCTGAC GGATACTGGC AAAAACGCCG 
     3061 TGAATTGTAC CTACAAGAAC GAGGATGACT GTGTCGTCAG ATTCCAGTAC TACGAAGACA 
     3121 CCAGTGGGAG GGCAGTCCTC TATGTGGTGG AAGAGCCTGA GTGTCCCAAG GGTCCTGATA 
     3181 TCCTGGTGGT CCTGCTGTCA GTGATGGGGG CCATCCTGCT CATTGGCCTT GCTACTCTGC 
     3241 TCATCTGGAA GCTACTCATC ACTATCCATG ACCGGAAGGA ATTTGCTAAA TTTGAGGAAG 
     3301 AACGAGCCAG AGCCAAGTGG GACACAGCAA ACAACCCGCT GTATAAAGAG GCCACCTCCA 
     3361 CCTTCACCAA TATCACCTAC CGGGGGACTA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     3421 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     3481 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     3541 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     3601 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     3661 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     3721 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     3781 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     3841 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     3901 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     3961 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     4021 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     4081 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     4141 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     4201 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     4261 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     4321 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     4381 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     4441 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     4501 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     4561 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     4621 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     4681 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     4741 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     4801 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     4861 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     4921 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     4981 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     5041 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     5101 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     5161 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     5221 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     5281 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     5341 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     5401 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     5461 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     5521 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     5581 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     5641 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     5701 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     5761 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     5821 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     5881 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     5941 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     6001 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     6061 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     6121 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     6181 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     6241 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     6301 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     6361 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     6421 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     6481 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     6541 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     6601 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     6661 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     6721 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     6781 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     6841 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     6901 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     6961 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     7021 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     7081 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     7141 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     7201 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//