LOCUS       MR227334                8499 bp    DNA     circular  SYN 
DEFINITION  Slc12a2 (Myc-DDK-tagged) - Mouse solute carrier family 12, member 2 (Slc12a2)
ACCESSION   NM_009194
VERSION     NM_009194.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4656..4747 
                     /label="Myc-DDK"  
     misc_feature    4777..5362 
                     /label="PolyA signal"  
     misc_feature    4834..4853 
                     /label="Restriction Enzyme"  
     misc_feature    5638..6310 
                     /label="ColE1"  
     misc_feature    6740..7534 
                     /label="Kan/Neo"  
     misc_feature    7569..7871 
                     /label="SV40 ori"  
     misc_feature    7957..8434 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4638..4644 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4638 
                     /label="Slc12a2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGCCCGGG CCCGCGGCGC CCTCGTCTGG CGCCCCGCGG CCCGCCCGGG 
     1081 ATGGGGACGC GCCGCTGGCT GCCGCCGCCG GGGTGGACCT GCCCGGCACG GCCGTGCCCT 
     1141 CCGGCCAAGA GGATGCTACT ACGGCTGGGA GGCAGGCTGG CGGCGGGGTG CGCGGAGAGG 
     1201 GAACCCCAGC GGCGGGCGAT GGGCTGGGCA GACCTCTGGG GCCCACCCCC AGCCAGAGCC 
     1261 GCTTCCAGGT GGACCCGGTT TCCGAGAACG CCGGGCGGGC CGCGGCGGCT GCGGCGGCGG 
     1321 CGGCGGCGGC TGCGGCTGCG GCCGGGGCGG CGGGCAAGGA GACCCCCGCG GCCGGGAAAG 
     1381 CCGGCGGTGA GAGCGGCGTG GCCAAGGGCA GCGAGGAAGC CAAGGGCCGC TTCCGCGTGA 
     1441 ACTTCGTGGA CCCGGCTGCC TCGTCGTCGG CGGACGACAG CCTGTCGGAT GCGGCGGGGG 
     1501 TCGGTGGCGA CGGGCCCAAC GTGAGCTTCC AGAACGGCGG GGACACGGTG CTGAGCGAGG 
     1561 GCAGCAGCCT GCACTCGGGG GGTGGCAGTG GACACCACCA GCAGTACTAC TATGACACCC 
     1621 ACACCAACAC CTACTACCTG CGCACCTTCG GCCACAACAC CATGGACGCG GTGCCCAGGA 
     1681 TCGACCACTA CCGGCACACG GCCGCGCAGC TGGGCGAGAA GCTGCTCCGG CCCAGCCTGG 
     1741 CAGAGCTCCA CGATGAGCTG GAAAAGGAAC CTTTTGAGGA TGGCTTTGCG AATGGAGAAG 
     1801 AAAGTACTCC AACCAGAGAT GCTGTGGTCG CATACACTGC CGAGAGTAAA GGAGTTGTAA 
     1861 AGTTTGGCTG GATCAAGGGT GTATTAGTCC GCTGCATGTT AAACATTTGG GGTGTGATGC 
     1921 TCTTTATTAG ATTGTCATGG ATCGTGGGTC AAGCTGGGAT AGGTCTCTCT GTCGTCGTAA 
     1981 TAGCAATGGC CACTGTTGTG ACAACTATCA CAGGATTGTC TACTTCAGCA ATAGCTACCA 
     2041 ATGGGTTCGT GAGAGGAGGA GGAGCGTACT ATTTAATCTC TAGAAGTCTA GGGCCGGAAT 
     2101 TTGGTGGTGC AATTGGCTTG ATCTTTGCTT TTGCCAATGC GGTGGCCGTT GCTATGTATG 
     2161 TTGTCGGATT CGCAGAGACT GTGGTGGAGC TGCTTAAGGA ACATTCCATA CTTATGATAG 
     2221 ATGAAATCAA TGATATCCGA ATTATTGGAG CCATTACAGT CGTGATTCTT CTGGGCATCT 
     2281 CAGTGGCTGG AATGGAGTGG GAAGCAAAGG CTCAGATTGT GCTGCTGGTG ATCCTTCTCC 
     2341 TGGCCATCGC CGACTTCGTC ATAGGGACGT TTATCTCACT GGAGAGCAAG AAGCCCAAGG 
     2401 GTTTCTTTGG CTATAAATCT GAAATATTTA ATGAGAACTT TGGGCCGGAT TTTCGAGAAG 
     2461 AAGAGACTTT CTTCTCTGTA TTTGCCATCT TTTTTCCTGC TGCAACTGGT ATTCTAGCTG 
     2521 GAGCAAATAT CTCAGGTGAT CTTGCAGATC CTCAGTCAGC CATACCCAAA GGAACACTCT 
     2581 TAGCCATTTT GATTACTACA GTGGTTTACA TAGGAATTGC AGTGTCTGTG GGTTCGTGTG 
     2641 TTGTTCGGGA TGCCACTGGC AATGTTAATG ATACCATTAC AACAGAGCTG ACAAACTGTA 
     2701 CTTCTGCAGC CTGCAAACTA AACTTTGACT TCTCTTATTG TGAGAGCAAT ACTTGTTCTT 
     2761 ACGGGCTAAT GAACAACTTT CAGGTGATGA GCATGGTGTC AGGATTTGCA CCCTTGATCT 
     2821 CTGCAGGGAT CTTTTCAGCC ACGCTCTCGT CAGCACTAGC ATCTCTTGTG AGTGCTCCCA 
     2881 AAATATTTCA GGCTCTATGT AAGGACAATA TCTACCCAGC TTTCCAGATG TTTGCTAAAG 
     2941 GTTATGGGAA AAATAATGAA CCGCTTCGTG GTTACATCTT AACATTTCTG ATTGCACTGG 
     3001 GATTCATCCT GATTGCTGAA TTGAACGTTA TTGCTCCAAT TATCTCTAAT TTCTTCCTTG 
     3061 CATCGTATGC CTTAATCAAC TTTTCAGTAT TCCATGCATC ACTTGCAAAA TCTCCAGGAT 
     3121 GGCGTCCTGC ATTCAAATAC TACAACATGT GGATCTCCCT TATTGGAGCA ATTCTTTGTT 
     3181 GCATAGTGAT GTTTGTCATT AACTGGTGGG CTGCGTTGCT CACATATGTG ATAGTCCTTG 
     3241 GGCTGTACAT TTATGTTACT TACAAAAAAC CAGATGTGAA CTGGGGATCC TCCACACAAG 
     3301 CGCTGACTTA CCTCAGTGCA CTGCAGCATT CAATCCGTCT TTCTGGGGTG GAGGACCATG 
     3361 TGAAAAACTT CAGACCACAG TGCCTTGTAA TGACAGGTTC TCCAAACTCA CGGCCTGCTT 
     3421 TACTTCATCT TGTTCATGAC TTCACGAAAA ATGTTGGTTT AATGATCTGT GGCCATGTGC 
     3481 ATATGGGCCC CAGAAGACAA GCTATGAAAG AGATGTCCAT TGATCAAGCC AGATATCAGC 
     3541 GATGGCTCAT TAAAAACAAA ATGAAGGCTT TCTATGCTCC GGTTCACGCA GATGACTTGC 
     3601 GAGAAGGTGC ACAATACTTG ATGCAGGCTG CTGGACTTGG TCGTATGAAG CCAAACACAC 
     3661 TTGTCCTGGG ATTTAAGAAA GATTGGTTAC AAGCCGATAT GAGGGATGTG GATATGTATA 
     3721 TAAACTTATT TCATGATGCT TTTGACATAC AATTTGGAGT TGTGGTTATC CGCCTGAAGG 
     3781 AAGGACTGGA TATATCACAC CTCCAAGGTC AGGAAGAATT ATTATCTTCA CAAGAAAAAT 
     3841 CACCTGGTAC CAAGGATGTG GTAGTAAATG TGGATTACAG TAAAAAGTCA GATCAAGATA 
     3901 CTTGCAAATC ATCTGGTGAA AAATCCATTA CACAGAAAGA TGAGGAAGAG GATGGCAAGA 
     3961 CTCCAACTCA GCCACTGTTG AAAAAAGAAT CCAAAGGCCC TATTGTGCCT TTAAATGTAG 
     4021 CTGACCAAAA GCTTCTTGAA GCTAGCACAC AGTTTCAGAA AAAACAAGGG AAGAATACTA 
     4081 TAGATGTCTG GTGGCTTTTT GATGATGGAG GTTTGACATT GTTGATACCC TATCTTCTGA 
     4141 CTACCAAGAA AAAGTGGAAA GATTGTAAGA TCCGAGTATT CATTGGTGGA AAAATAAACA 
     4201 GAATAGACCA TGACCGGAGA GCGATGGCTA CTTTATTGAG TAAATTCCGG ATAGACTTCT 
     4261 CTGATATCAT GGTCCTTGGA GATATCAACA CCAAGCCAAA AAAAGAAAAT ATTATTGCTT 
     4321 TTGATGACAT GATCGAGCCG TACAGACTTC ACGAAGATGA TAAAGAACAG GACATTGCAG 
     4381 ATAAGATGAA AGAAGATGAG CCTTGGCGAA TCACAGATAA TGAGCTTGAG CTTTATAAGA 
     4441 CTAAGACATA TCGACAGATC AGGTTAAATG AATTATTAAA GGAGCATTCA AGCACAGCTA 
     4501 ACATTATTGT CATGAGTCTT CCAGTCGCAC GGAAAGGCGC TGTGTCCAGT GCTCTGTATA 
     4561 TGGCTTGGTT AGAAGCTCTC TCGAAGGACC TGCCACCAGT CCTCCTGGTT CGTGGGAATC 
     4621 ATCAGAGCGT CCTTACCTTC TACTCAACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     4681 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     4741 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     4801 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     4861 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     4921 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     4981 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     5041 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     5101 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     5161 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     5221 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     5281 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     5341 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     5401 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     5461 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     5521 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     5581 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     5641 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     5701 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     5761 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     5821 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     5881 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     5941 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     6001 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     6061 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     6121 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     6181 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     6241 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     6301 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     6361 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     6421 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     6481 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     6541 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     6601 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     6661 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     6721 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     6781 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     6841 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     6901 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     6961 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     7021 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     7081 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     7141 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     7201 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     7261 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     7321 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     7381 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     7441 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     7501 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     7561 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     7621 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     7681 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     7741 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7801 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7861 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     7921 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     7981 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     8041 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     8101 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     8161 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     8221 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     8281 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     8341 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     8401 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     8461 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//