LOCUS       MR227324                8340 bp    DNA     circular  SYN 
DEFINITION  Itgam (Myc-DDK-tagged) - Mouse integrin alpha M (Itgam), transcript variant 2
ACCESSION   NM_008401
VERSION     NM_008401.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4497..4588 
                     /label="Myc-DDK"  
     misc_feature    4618..5203 
                     /label="PolyA signal"  
     misc_feature    4675..4694 
                     /label="Restriction Enzyme"  
     misc_feature    5479..6151 
                     /label="ColE1"  
     misc_feature    6581..7375 
                     /label="Kan/Neo"  
     misc_feature    7410..7712 
                     /label="SV40 ori"  
     misc_feature    7798..8275 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4479..4485 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4479 
                     /label="Itgam"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACTCTTAAA GCTCTTCTGG TCACAGCCCT AGCCTTGTGT CATGGCTTCA 
     1081 ATCTGGACAC TGAACATCCC ATGACCTTCC AAGAGAATGC AAAAGGCTTT GGACAGAGTG 
     1141 TGGTCCAGCT TGGCGGAACC AGTGTGGTTG TTGCAGCCCC CCAGGAGGCA AAGGCTGTTA 
     1201 ACCAGACAGG TGCCCTCTAC CAGTGTGACT ACAGCACAAG CCGGTGTCAC CCCATCCCCC 
     1261 TGCAAGTACC TCCAGAGGCT GTGAATATGT CCTTGGGCCT GTCCCTGGCT GTTTCTACTG 
     1321 TCCCCCAGCA GCTGCTGGCC TGTGGCCCCA CGGTGCACCA AAACTGCAAG GAGAATACTT 
     1381 ATGTGAATGG ATTGTGCTAT TTGTTCGGCT CCAACCTGCT GAGGCCGCCC CAGCAGTTCC 
     1441 CAGAGGCTCT CAGAGAATGT CCTCAGCAGG AGAGTGACAT TGTCTTCTTG ATTGATGGCT 
     1501 CCGGTAGCAT CAACAACATT GACTTTCAGA AGATGAAGGA GTTTGTCTCA ACTGTGATGG 
     1561 AGCAGTTCAA AAAGTCTAAA ACCTTGTTCT CTTTGATGCA GTACTCGGAC GAGTTCCGGA 
     1621 TTCACTTCAC CTTCAATGAC TTCAAGAGAA ACCCTAGCCC AAGATCACAT GTGAGCCCCA 
     1681 TAAAGCAGCT GAATGGGAGG ACAAAAACTG CCTCAGGGAT CCGGAAAGTA GTGAGAGAAC 
     1741 TGTTTCACAA AACCAATGGG GCCCGGGAGA ATGCTGCGAA GATCCTAGTT GTCATCACAG 
     1801 ATGGAGAAAA ATTCGGTGAT CCCTTGGATT ATAAGGATGT CATCCCCGAG GCAGACAGAG 
     1861 CAGGGGTCAT TCGCTACGTA ATTGGGGTGG GAAATGCCTT CAACAAACCA CAGTCCCGCA 
     1921 GAGAGCTCGA CACCATCGCA TCTAAGCCAG CTGGTGAACA CGTGTTCCAA GTGGACAACT 
     1981 TTGAAGCCCT GAATACCATT CAGAACCAGC TTCAGGAAAA GATCTTTGCA ATTGAGGGCA 
     2041 CGCAGACAGG AAGTACCAGC TCCTTTGAGC ATGAGATGTC TCAAGAAGGC TTCAGTGCTT 
     2101 CCATTACCTC TAATGGTCCC TTGCTGGGCT CTGTGGGGAG CTTTGACTGG GCAGGTGGAG 
     2161 CCTTCCTGTA TACATCGAAG GATAAAGTCA CCTTCATCAA CACAACCAGA GTGGATTCAG 
     2221 ACATGAATGA TGCTTACCTG GGTTATGCTT CTGCAGTCAT CTTGAGGAAC CGTGTCCAAA 
     2281 GCTTGGTTTT AGGAGCACCT CGGTATCAGC ATATTGGCTT GGTGGTGATG TTCAGGGAGA 
     2341 ATTTCGGTAC CTGGGAGCCC CACACTAGCA TCAAGGGCAG CCAGATTGGC TCTTATTTTG 
     2401 GGGCCTCCCT TTGCTCTGTG GACATGGACG CTGATGGCAA TACCAACTTG ATCCTCATTG 
     2461 GGGCCCCTCA TTACTATGAG AAGACCCGAG GAGGCCAGGT GTCAGTGTGT CCCTTGCCTC 
     2521 GAGGGAGGGC ACGGTGGCAG TGTGAAGCTC TTCTCCACGG TGATCAGGGT CATCCCTGGG 
     2581 GTCGCTTTGG GGCAGCCCTG ACAGTGCTGG GAGACGTGAA TGGGGACAAA CTGACAGATG 
     2641 TGGCCATTGG GGCCCCAGGA GAGCAGGAGA ATCAGGGCGC TGTCTACATT TTTTATGGAG 
     2701 CATCAATAGC CAGCCTCAGT GCCTCCCACA GCCAGCGGAT CATAGGCGCC CACTTCTCCC 
     2761 CTGGGCTCCA GTACTTCGGG CAGTCTCTGA GTGGGGGTAA GGATCTCACA ATGGATGGCT 
     2821 TGATGGACCT GGCTGTTGGG GCGCAGGGGC ATCTTCTGCT GCTCAGAGCC CAGCCGGTGC 
     2881 TGAGACTGGA GGCAACCATG GAATTCAGCC CCAAGAAAGT AGCAAGGAGT GTGTTTGCAT 
     2941 GTCAAGAACA AGTATTAAAA AACAAGGATG CTGGGGAGGT CAGAGTCTGC CTCCGTGTCC 
     3001 GCAAGAACAC CAAGGACCGT CTGCGCGAAG GAGATATCCA GAGCACTGTC ACTTATGACC 
     3061 TGGCTTTAGA CCCTGGCCGC TCACGTATCC GTGCCTTCTT TGATGAGACA AAGAACAACA 
     3121 CACGCAGGCG CACCCAGGTC TTTGGATTGA TGCAGAAATG TGAAACACTG AAGCTAATTT 
     3181 TACCGGACTG CGTGGACGAC TCAGTGAGCC CCATCATCCT GCGCCTCAAT TATACACTGG 
     3241 TTGGGGAGCC CTTGAGGTCC TTTGGGAACC TCCGACCAGT TCTGGCTATG GATGCTCAGA 
     3301 GGTTCTTCAC AGCTATGTTT CCCTTTGAGA AAAATTGCGG CAATGACAGC ATCTGCCAAG 
     3361 ACGATCTCAG CATCACCATG AGTGCCATGG GCTTGGACAC TTTGGTGGTG GGAGGCCCCC 
     3421 AGGACTTTAA CATGAGTGTG ACTCTGAGAA ATGACGGTGA GGATTCCTAC GGGACCCAGG 
     3481 TTACCGTCTA CTACCCATCT GGCTTATCTT ACCGGAAGGA TTCAGCAAGC CAGAACCCGC 
     3541 TCACCAAGAA GCCTTGGTTT GTAAAGCCTG CTGAGTCCAG CTCTTCTTCT GAAGGGCATG 
     3601 GGGCTCTGAA GAGTACCACC TGGAACATAA ACCATCCCAT CTTTCCTGCT AATTCTGAGG 
     3661 TCACATTTAA TGTCACATTT GATGTGGACT CTCATGCCTC CTTTGGGAAC AAACTGCTCC 
     3721 TCAAAGCCAT TGTGGCCAGT GAGAACAACA TGTCCAGGAC CCACAAAACC AAGTTTCAGT 
     3781 TGGAGCTGCC TGTGAAGTAC GCCATCTACA TGATTGTCAC CAGTGATGAG AGTTCTATCA 
     3841 GATATCTCAA CTTCACGGCT TCAGAGATGA CCAGTAAGGT CATACAGCAT CAGTACCAGT 
     3901 TCAACAACCT GGGCCAGAGG AGCCTCCCTG TCAGTGTAGT CTTCTGGATC CCTGTTCAGA 
     3961 TCAACAATGT GACCGTATGG GATCATCCCC AGGTCATCTT CTCCCAGAAC CTCTCAAGTG 
     4021 CCTGTCACAC TGAGCAGAAA TCCCCCCCTC ACTCCAATTT CCGGGATCAG CTTGAAAGGA 
     4081 CCCCAGTGCT GAACTGCTCT GTTGCAGTCT GTAAGAGAAT TCAGTGTGAC CTTCCATCCT 
     4141 TCAACACTCA GGAAATATTC AATGTCACCC TCAAGGGCAA CCTATCATTT GACTGGTACA 
     4201 TCAAGACTTC TCATGGTCAC CTCCTGCTTG TGAGCAGCAC TGAGATCCTG TTTAATGACT 
     4261 CTGCGTTTGC CCTGCTTCCA GGGCAGGAGT CGTATGTGAG GTCTAAGACA GAGACCAAAG 
     4321 TGGAGCCATA TGAAGTTCAC AATCCTGTAC CACTCATTGT GGGCAGCTCC ATTGGGGGCC 
     4381 TGGTGCTCTT GGCTCTCATC ACTGCTGGCC TATACAAGCT TGGCTTTTTC AAGCGGCAGT 
     4441 ACAAGGACAT GATGAATGAA GCTGCCCCCC AAGACGCCCC ACCTCAGACG CGTACGCGGC 
     4501 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     4561 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     4621 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     4681 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     4741 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     4801 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     4861 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     4921 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     4981 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     5041 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     5101 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     5161 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     5221 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     5281 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     5341 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     5401 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     5461 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     5521 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     5581 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     5641 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     5701 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     5761 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     5821 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     5881 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     5941 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     6001 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     6061 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     6121 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     6181 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     6241 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     6301 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     6361 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     6421 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     6481 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     6541 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     6601 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     6661 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     6721 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     6781 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     6841 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     6901 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     6961 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     7021 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     7081 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     7141 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     7201 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     7261 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     7321 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     7381 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     7441 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     7501 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     7561 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     7621 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     7681 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     7741 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     7801 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     7861 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     7921 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     7981 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     8041 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     8101 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     8161 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     8221 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     8281 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//