LOCUS       MR227301                8640 bp    DNA     circular  SYN 
DEFINITION  Ltbp3 (Myc-DDK-tagged) - Mouse latent transforming growth factor beta binding protein 3 (Ltbp3)
ACCESSION   NM_008520
VERSION     NM_008520.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4797..4888 
                     /label="Myc-DDK"  
     misc_feature    4918..5503 
                     /label="PolyA signal"  
     misc_feature    4975..4994 
                     /label="Restriction Enzyme"  
     misc_feature    5779..6451 
                     /label="ColE1"  
     misc_feature    6881..7675 
                     /label="Kan/Neo"  
     misc_feature    7710..8012 
                     /label="SV40 ori"  
     misc_feature    8098..8575 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4779..4785 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4779 
                     /label="Ltbp3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCCGGGCCC CGAGGGGCTG CCCACGGCCT GGCCCCTGCG ATGCGCCAGG 
     1081 CCGGGGCATT GGGGCTGCTG GCACTACTCC TGCTGGCGCT GCTGGGCCCC GGCGGCGGGG 
     1141 CCGAGGGTGG GCCGGCCGGC GAGCGGGGCA CAGGCGGGGG CGGGGCGCTG GCCCGCGAAC 
     1201 GCTTCAAGGT GGTCTTTGCG CCAGTGATCT GCAAGCGGAC CTGTCTGAAG GGCCAGTGTC 
     1261 GGGACAGCTG TCAGCAGGGC TCCAACATGA CGCTCATCGG AGAGAACGGC CACAGCACCG 
     1321 ACACGCTCAC CGGTTCTGGC TTCCGCGTGG TGGTGTGCCC TCTACCCTGC ATGAACGGTG 
     1381 GCCAGTGCTC TTCCCGAAAC CAGTGCCTGT GTCCCCCGGA TTTCACGGGG CGCTTCTGCC 
     1441 AGGTGCCTGC TGCAGGAACC GGAGCTGGCA CCGGGAGTTC AGGCCCCGGC CTGGCCCGGA 
     1501 CCGGGGCCAT GTCCACAGGC CCGCTGCCGC CCCTTGCCCC AGAAGGAGAG TCTGTGGCTA 
     1561 GCAAACACGC CATTTACGCG GTGCAGGTGA TCGCAGATCC TCCCGGGCCG GGGGAGGGTC 
     1621 CTCCTGCACA ACATGCAGCC TTCTTGGTGC CCCTGGGGCC AGGACAAATC TCGGCAGAAG 
     1681 TGCAGGCTCC GCCCCCCGTG GTGAACGTGC GTGTCCATCA CCCTCCTGAA GCTTCCGTTC 
     1741 AGGTGCACCG CATCGAGGGG CCGAACGCTG AAGGCCCAGC CTCTTCCCAG CACTTGCTGC 
     1801 CGCATCCCAA GCCCCAGCAC CCGAGGCCAC CCACCCAAAA GCCACTGGGC CGCTGCTTCC 
     1861 AGGACACATT GCCCAAGCAG CCTTGTGGCA GCAACCCTTT GCCTGGCCTT ACCAAGCAGG 
     1921 AAGATTGCTG CGGTAGCATC GGTACTGCCT GGGGACAAAG CAAGTGTCAC AAGTGCCCAC 
     1981 AGCTTCAGTA TACAGGGGTG CAGAAGCCTG GACCTGTACG TGGGGAGGTG GGTGCTGACT 
     2041 GCCCCCAGGG CTACAAGAGG CTCAACAGCA CCCACTGCCA GGATATCAAC GAATGTGCGA 
     2101 TGCCTGGCAT GTGTCGCCAT GGTGACTGCC TCAACAACCC TGGCTCTTAT CGCTGTGTCT 
     2161 GCCCGCCCGG TCATAGCTTG GGTCCCTCGC GCACACAGTG CATTGCCGAC AAACCAGAGG 
     2221 AGAAGAGCCT GTGTTTCCGC CTTGTGAGCA CCGAACACCA GTGCCAGCAC CCTCTGACCA 
     2281 CACGCCTAAC CCGCCAGCTC TGCTGCTGTA GTGTGGGTAA AGCCTGGGGT GCCCGGTGCC 
     2341 AGCGCTGCCC GGCAGATGGT ACAGCAGCCT TCAAGGAGAT CTGCCCGGCT GGGAAAGGGT 
     2401 ACCATATCCT CACCTCCCAC CAGACGCTCA CCATCCAGGG GGAAAGCGAC TTCTCCCTCT 
     2461 TCCTGCACCC CGACGGGCCA CCCAAACCCC AGCAGCTTCC TGAAAGCCCC AGCCGAGCAC 
     2521 CACCCCTCGA GGACACAGAG GAAGAGAGAG GAGTGACCAT GGATCCACCA GTGAGTGAGG 
     2581 AGCGATCGGT GCAGCAGAGC CACCCCACTA CCACCACCTC ACCCCCCCGG CCTTACCCAG 
     2641 AGCTCATCTC TCGCCCCTCC CCACCTACCT TCCACCGGTT CCTGCCAGAC TTGCCCCCAT 
     2701 CCCGAAGTGC AGTGGAGATC GCCCCCACTC AGGTCACAGA GACCGATGAG TGCCGATTGA 
     2761 ACCAGAATAT CTGTGGCCAT GGACAGTGTG TGCCTGGCCC CTCGGATTAC TCCTGCCACT 
     2821 GCAACGCTGG CTACCGGTCA CACCCGCAGC ACCGCTACTG TGTTGATGTG AACGAGTGCG 
     2881 AGGCAGAGCC CTGCGGCCCC GGGAAAGGCA TCTGTATGAA CACTGGTGGC TCCTACAATT 
     2941 GTCACTGCAA CCGAGGCTAC CGCCTCCACG TGGGTGCAGG GGGCCGCTCG TGCGTGGACC 
     3001 TGAACGAGTG CACCAAGCCT CACCTGTGTG GGGACGGTGG CTTCTGCATC AACTTCCCTG 
     3061 GTCACTACAA ATGCAACTGC TATCCTGGCT ACCGGCTCAA GGCCTCCCGA CCGCCCATTT 
     3121 GCGAAGACAT CGACGAGTGT CGCGACCCTA GCACCTGCCC TGATGGCAAA TGTGAAAACA 
     3181 AACCTGGCAG CTTCAAGTGC ATCGCCTGCC AGCCTGGCTA CCGTAGCCAG GGGGGCGGGG 
     3241 CCTGTCGTGA TGTCAACGAA TGCTCCGAGG GTACCCCCTG CTCTCCTGGA TGGTGTGAGA 
     3301 ACCTTCCGGG TTCTTACCGT TGCACGTGTG CCCAGGGATA CGAACCCGCA CAGGACGGCC 
     3361 TCAGTTGCAT AGACGTGGAT GAGTGTGAGG CTGGGAAAGT GTGCCAAGAT GGCATCTGCA 
     3421 CGAACACACC AGGCTCTTTC CAGTGTCAGT GCCTCTCCGG CTATCATCTG TCAAGGGATC 
     3481 GGAGCCGCTG TGAGGACATT GATGAATGTG ACTTCCCTGC GGCCTGCATC GGGGGTGACT 
     3541 GCATCAATAC CAATGGTTCC TACAGATGTC TCTGTCCCCA GGGTCATCGG TTGGTGGGCG 
     3601 GTAGGAAGTG CCAAGATATA GATGAGTGCA GCCAGGACCC AGGCCTGTGC CTGCCCCACG 
     3661 GGGCCTGCGA GAACCTCCAG GGCTCCTATG TCTGTGTCTG TGATGAGGGT TTCACACTCA 
     3721 CCCAGGACCA GCATGGGTGT GAGGAGGTGG AGCAGCCCCA CCACAAGAAG GAGTGCTACC 
     3781 TTAACTTCGA TGACACAGTG TTCTGTGACA GCGTATTGGC TACCAATGTC ACTCAGCAGG 
     3841 AATGCTGTTG CTCTCTGGGA GCTGGTTGGG GAGACCACTG CGAAATCTAT CCCTGTCCAG 
     3901 TCTACAGCTC AGCCGAATTT CACAGCCTGT GTCCTGATGG GAAAGGCTAC ACTCAGGACA 
     3961 ACAACATTGT GAACTATGGC ATTCCTGCCC ACCGTGACAT CGACGAATGC ATATTGTTTG 
     4021 GGGCAGAGAT CTGCAAGGAG GGCAAGTGTG TGAACACGCA GCCCGGCTAC GAGTGCTACT 
     4081 GCAAGCAGGG CTTCTACTAC GATGGCAACC TGCTGGAGTG CGTGGACGTG GATGAGTGCT 
     4141 TGGATGAGTC TAACTGCAGG AACGGAGTGT GTGAGAACAC ACGTGGCGGC TACCGCTGTG 
     4201 CCTGCACGCC GCCGGCAGAG TACAGTCCCG CACAGCGCCA GTGTCTGAGC CCGGAGGAGA 
     4261 TGGAGCACGC CCCAGAGAGA CGTGAAGTGT GCTGGGGCCA GCGAGGAGAG GACGGCATGT 
     4321 GTATGGGGCC CCTGGCGGGA CCTGCCCTCA CTTTTGATGA CTGCTGCTGC CGCCAGGGCC 
     4381 GCGGCTGGGG TACCCAGTGC AGACCGTGCC CGCCACGTGG CACCGGGTCC CAGTGCCCGA 
     4441 CTTCACAGAG TGAGAGCAAT TCATTCTGGG ACACAAGCCC CCTGCTACTG GGGAAGTCTC 
     4501 CGCGAGACGA AGACAGCTCA GAGGAGGATT CAGATGAGTG CCGTTGTGTG AGCGGACGCT 
     4561 GTGTGCCACG GCCAGGCGGG GCGGTATGCG AGTGTCCTGG AGGCTTTCAG CTGGACGCCT 
     4621 CCCGTGCCCG CTGCGTGGAC ATTGATGAGT GCCGAGAACT GAACCAGCGG GGACTACTGT 
     4681 GTAAGAGCGA GCGGTGCGTG AACACCAGTG GATCCTTCCG CTGTGTCTGC AAAGCTGGCT 
     4741 TCACGCGCAG CCGCCCTCAC GGGGCCTGCG TGCCTCAGCG CCGCCGCACG CGTACGCGGC 
     4801 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     4861 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     4921 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     4981 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     5041 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     5101 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     5161 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     5221 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     5281 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     5341 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     5401 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     5461 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     5521 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     5581 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     5641 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     5701 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     5761 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     5821 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     5881 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     5941 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     6001 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     6061 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     6121 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     6181 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     6241 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     6301 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     6361 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     6421 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     6481 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     6541 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     6601 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     6661 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     6721 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     6781 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     6841 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     6901 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     6961 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     7021 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     7081 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     7141 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     7201 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     7261 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     7321 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     7381 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     7441 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     7501 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     7561 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     7621 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     7681 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     7741 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     7801 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     7861 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     7921 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     7981 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     8041 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     8101 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     8161 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     8221 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     8281 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     8341 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     8401 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     8461 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     8521 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     8581 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//