LOCUS       MR227232                8394 bp    DNA     circular  SYN 
DEFINITION  Pcdh15 (Myc-DDK-tagged) - Mouse protocadherin 15 (Pcdh15), transcript variant N
ACCESSION   NM_001142748
VERSION     NM_001142748.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4551..4642 
                     /label="Myc-DDK"  
     misc_feature    4672..5257 
                     /label="PolyA signal"  
     misc_feature    4729..4748 
                     /label="Restriction Enzyme"  
     misc_feature    5533..6205 
                     /label="ColE1"  
     misc_feature    6635..7429 
                     /label="Kan/Neo"  
     misc_feature    7464..7766 
                     /label="SV40 ori"  
     misc_feature    7852..8329 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4533..4539 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4533 
                     /label="Pcdh15"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTTCCTACAG TTTGCTGTCT GGAAGTGTTT ACCCCATGGG ATCCTCATTG 
     1081 CCTCTCTCTT GGTAGTCAGC TGGGGCCAGT ATGACGATGA TTGCAAACTA GCTAGGGGAG 
     1141 GACCACCAGC TACTATCGTG GCCATTGATG AAGAGAGTCG AAACGGTACA ATTCTGGTGG 
     1201 ATAACATGTT GATTAAGGGG ACTGCCGGAG GACCAGACCC CACCATAGAG CTCTCTTTAA 
     1261 AGGACAACGT GGACTACTGG GTGTTGCTGG ACCCCGTTAA ACAGATGCTT TTCCTGAACA 
     1321 GTACCGGAAG AGTTCTGGAT AGAGACCCAC CAATGAACAT ACACTCCATT GTGGTGCAAG 
     1381 TCCAGTGTGT CAACAAGAAG GTTGGCACAG TTATCTATCA TGAAGTACGC ATCGTGGTGC 
     1441 GAGATCGGAA TGACAACTCC CCCACATTCA AGCATGAAAG CTACTATGCC ACCGTGAATG 
     1501 AGCTCACTCC AGTTGGCACC ACGATATTCA CGGGGTTCTC GGGAGACAAT GGAGCTACAG 
     1561 ACATAGACGA TGGCCCTAAT GGACAGATAG AATACGTGAT TCAGTACAAC CCAGAAGATC 
     1621 CGACATCCAA CGACACCTTT GAAATTCCAC TCATGCTGAC TGGCAACGTG GTACTGAGGA 
     1681 AAAGACTCAA CTATGAGGAT AAGACTCGCT ACTATGTCAT CATCCAAGCA AATGACCGTG 
     1741 CACAAAATCT GAATGAGAGG CGAACAACCA CCACCACCCT CACAGTAGAT GTTCTAGATG 
     1801 GAGATGACCT GGGACCTATG TTTCTGCCTT GTGTTCTTGT GCCAAACACA CGTGACTGTC 
     1861 GTCCACTCAC CTACCAAGCT GCCATTCCTG AACTGAGGAC TCCGGAAGAA CTGAACCCTA 
     1921 TTTTGGTGAC ACCACCTATC CAAGCCATTG ATCAGGACCG AAACATCCAA CCACCATCTG 
     1981 ATCGACCTGG CATCCTCTAC TCCATCCTTG TCGGCACCCC TGAGGATTAC CCCCGCTTCT 
     2041 TCCATATGCA TCCCAGGACT GCAGAACTCA CTCTCCTGGA GCCAGTAAAC AGAGACTTCC 
     2101 ATCAAAAATT TGATTTGGTT ATTAAGGCTG AGCAGGACAA TGGCCACCCA CTTCCTGCCT 
     2161 TTGCTAGTCT GCACATCGAA ATACTAGACG AAAACAATCA GAGTCCATAC TTCACAATGC 
     2221 CCAGCTATCA AGGATACATC CTGGAATCCG CCCCAGTGGG AGCCACCATT TCTGAGAGCC 
     2281 TAAACTTAAC CACTCCTCTG AGAATTGTAG CTCTGGACAA AGACATAGAA GACACAAAAG 
     2341 ATCCAGAGCT CCACCTCTTC CTGAATGACT ACACCTCGGT CTTCACTGTG ACACCCACTG 
     2401 GTATCACCCG CTACCTCACC CTGCTTCAAC CTGTGGACAG GGAGGAACAG CAAACCTACA 
     2461 CCTTTCTGAT AACAGCGTTT GATGGCGTGC AAGAAAGTGA GCCAGTCGTG GTCAATATCC 
     2521 GAGTGATGGA TGCAAATGAT AACACGCCCA CCTTCCCTGA AATCTCCTAT GATGTCTATG 
     2581 TTTACACAGA CATGAGTCCT GGGGACAGCG TCATTCAGCT GACAGCGGTA GATGCTGATG 
     2641 AAGGCTCTAA TGGGGAGATC TCCTATGAAA TACTGGTGGG GGGCAAGGGA GACTTCGTGA 
     2701 TCAACAAGAC CACAGGGCTG GTGAGCATTG CACCAGGCGT GGAGCTGATC GTGGGACAGA 
     2761 CGTATGCGCT CACAGTGCAG GCTTCGGACA ACGCCCCGCC TGCAGAAAGA AGGCACTCCA 
     2821 TCTGCACAGT GTACATCGAG GTGCTTCCTC CTAACAACCA GAGCCCTCCC CGCTTCCCGC 
     2881 AGCTGATGTA CAGTCTGGAA GTCAGCGAGG CCATGAGGAT CGGTGCTATT TTATTAAATC 
     2941 TACAGGCAAC TGATCGAGAG GGAGATCCAA TCACATATGC CATCGAGAAT GGAGACCCTC 
     3001 AGAGAGTTTT TAATCTTTCA GAAACCACAG GGATTCTCAG CCTAGGGAAG GCTCTAGACC 
     3061 GCGAGAGCAC AGACCGCTAC ATCCTCATCG TCACAGCCTC AGATGGCAGA CCGGATGGAA 
     3121 CCTCAACTGC CACTGTGAAC ATAGTGGTGA CGGACGTCAA TGACAACGCT CCCGTGTTCG 
     3181 ATCCCTATCT GCCCAGGAAC CTCTCTGTGG TGGAGGAAGA AGCCAATGCC TTTGTGGGTC 
     3241 AAGTCCGGGC AACAGACCCA GATGCTGGGA TAAACGGCCA AGTTCACTAC AGCCTGGGGA 
     3301 ACTTCAACAA CCTCTTCCGC ATCACATCCA ACGGGAGCAT TTACACAGCC GTGAAGCTGA 
     3361 ACAGGGAAGC CAGGGACCAC TATGAACTGG TTGTCGTGGC AACAGATGGA GCAGTCCACC 
     3421 CTCGACATTC AACTCTGACA CTGTACATCA AGGTGTTGGA CATTGATGAT AACAGTCCTG 
     3481 TTTTTACCAA TTCAACGTAC ACAGTTGTCG TTGAAGAGAA TCTGCCAGCC GGGACCTCCT 
     3541 TTCTTCAAAT AGAGGCCAAG GATGTTGACC TTGGAGCCAA TGTGTCATAT CGGATCAGAA 
     3601 GCCCAGAAGT GAAACACCTT TTTGCACTGC ATCCATTCAC TGGAGAATTG TCTCTTCTGA 
     3661 GGAGTTTGGA TTATGAGGCC TTTCCGGACC AGGAGGCAAG CATCACATTC TTGGTGGAGG 
     3721 CCTTTGACAT TTATGGGACT ATGCCACCTG GTATAGCAAC AGTCACGGTA ATTGTGAAGG 
     3781 ACATGAATGA CTACCCTCCA GTGTTTAGCA AACGCATCTA CAAGGGGATG GTGGCTCCAG 
     3841 ATGCAGTCAA GGGGACACCA ATCACCACCG TTTATGCTGA AGATGCGGAC CCACCTGGGA 
     3901 TGCCTGCAAG TAGGGTGAGG TATCGAGTGG ACGACGTGCA GTTTCCATAC CCAGCCAGTA 
     3961 TTTTTGATGT AGAGGAAGAT TCTGGAAGAG TAGTAACCCG CGTCAATCTT AATGAAGAGC 
     4021 CTACTACGAT TTTCAAGCTG GTGGTTGTGG CTTTTGATGA CGGCGAACCT GTGATGTCCA 
     4081 GCAGTGCCAC GGTGAGAATT CTTGTCTTAC ATCCTGGAGA GATCCCACGC TTCACCCAAG 
     4141 AGGAATACAG ACCTCCTCCT GTAAGTGAGC TTGCGGCCAG AGGGACTGTA GTTGGTGTCA 
     4201 TTTCTGCTGC TGCCATTAAT CAGAGCATCG TGTACTCCAT TGTGGCAGGA AATGAGGAAG 
     4261 ACAAGTTTGG AATCAACAAT GTCACTGGGG TCATCTATGT GAATTCACCA TTGGATTACG 
     4321 AGACAAGGAC CAGCTATGTG CTCCGGGTAC AAGCAGATTC TCTGGAAGTG GTCCTTGCCA 
     4381 ATCTCCGAGT CCCTTCAAAA AGTCTGTCTG TGATACCATG TTCATGGAGA ACCCAGGTGT 
     4441 CAAAGTCTCT AGGACTGGAG CTAGGGGTGC CTGTGAGCCA CAGTGTGGAA TCTGGAACTA 
     4501 GAACTGGGTC CTCTACAAGA GCAGCAAGTG TTCCTATCCA CACGCGTACG CGGCCGCTCG 
     4561 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     4621 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     4681 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     4741 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     4801 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     4861 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     4921 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     4981 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     5041 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     5101 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     5161 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     5221 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     5281 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     5341 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     5401 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     5461 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     5521 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     5581 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     5641 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     5701 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     5761 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     5821 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     5881 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     5941 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     6001 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     6061 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     6121 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     6181 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     6241 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     6301 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     6361 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     6421 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     6481 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     6541 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     6601 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     6661 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     6721 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     6781 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     6841 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     6901 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     6961 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     7021 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     7081 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     7141 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     7201 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     7261 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     7321 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     7381 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     7441 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     7501 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     7561 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     7621 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7681 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     7741 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     7801 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     7861 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     7921 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     7981 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     8041 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     8101 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     8161 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     8221 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     8281 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     8341 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//