LOCUS       MR227167                8844 bp    DNA     circular  SYN 
DEFINITION  Irs2 (Myc-DDK-tagged) - Mouse insulin receptor substrate 2 (Irs2)
ACCESSION   NM_001081212
VERSION     NM_001081212.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5001..5092 
                     /label="Myc-DDK"  
     misc_feature    5122..5707 
                     /label="PolyA signal"  
     misc_feature    5179..5198 
                     /label="Restriction Enzyme"  
     misc_feature    5983..6655 
                     /label="ColE1"  
     misc_feature    7085..7879 
                     /label="Kan/Neo"  
     misc_feature    7914..8216 
                     /label="SV40 ori"  
     misc_feature    8302..8779 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4983..4989 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4983 
                     /label="Irs2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTAGCGCG CCCCTGCCTG GGCCCCCCGC GTCGGCGGGC GGGGACGGCC 
     1081 CGAACCTCAA TAACAACAAC AACAACAACA ACCACAGCGT GCGCAAGTGC GGCTACCTGC 
     1141 GCAAGCAGAA GCACGGCCAC AAGCGCTTTT TCGTGTTGCG CGGCCCCGGC ACGGGCGGCG 
     1201 ACGAGGCATC CGCGGCTGGG GGGTCGCCGC CGCAGCCTCC GCGGCTGGAG TACTACGAGA 
     1261 GCGAGAAGAA GTGGAGGAGC AAGGCGGGCG CGCCGAAGCG AGTGATCGCG CTCGACTGCT 
     1321 GTCTGAACAT CAACAAGCGC GCGGACGCCA AGCACAAGTA CCTGATCGCC CTCTACACCA 
     1381 AGGACGAGTA CTTCGCTGTA GCGGCGGAGA ACGAGCAGGA GCAGGAGGGC TGGTACCGCG 
     1441 CACTCACCGA CTTGGTCAGC GAAGGCCGCT CTGGCGAGGG GGGCTCGGGC ACCACCGGAG 
     1501 GCTCTTGCAG CGCCTCTCTC CCGGGCGTCC TGGGCGGCTC AGCGGGCGCC GCTGGCTGCG 
     1561 ATGACAACTA CGGGCTCGTG ACACCCGCCA CGGCCGTCTA CCGCGAGGTG TGGCAGGTGA 
     1621 ACCTGAAACC TAAGGGACTG GGCCAGAGCA AGAACCTGAC TGGTGTATAC CGCCTATGCC 
     1681 TGTCTGCGCG CACCATCGGC TTCGTGAAGC TCAATTGCGA ACAGCCGTCG GTGACGCTGC 
     1741 AGCTTATGAA CATTCGCCGC TGCGGCCACT CGGACAGCTT CTTCTTCATC GAGGTGGGCC 
     1801 GTTCGGCCGT CACCGGTCCC GGGGAGCTGT GGATGCAAGC CGACGACTCG GTGGTGGCGC 
     1861 AGAACATCCA TGAGACCATC CTAGAAGCTA TGAAGGCACT CAAAGAGCTC TTCGAGTTCC 
     1921 GGCCTCGCAG CAAGAGTCAG TCGTCCGGGT CGTCAGCCAC GCATCCCATC AGCGTGCCGG 
     1981 GCGCGCGCCG CCACCACCAC CTAGTCAACC TACCCCCTAG CCAGACCGGC CTGGTGCGCC 
     2041 GCTCGCGCAC TGACAGCCTG GCGGCCACCC CCCCAGCAGC CAAGTGCACT TCGTGCCGGG 
     2101 TTCGTACGGC CAGCGAGGGC GACGGCGGCG CGGCAGGCGG GGCCGGGACG GCAGGAGGCA 
     2161 GGCCGATGTC GGTGGCAGGG AGCCCCCTGA GTCCCGGGCC GGTGCGCGCG CCCCTTAGCC 
     2221 GCTCGCACAC CCTGAGCGCC GGCTGCGGAG GCCGCCCGAG CAAAGTGACT CTGGCGCCGG 
     2281 CAGGGGGAGC CCTGCAACAC AGCCGCTCCA TGTCCATGCC CGTGGCGCAC TCACCTCCTG 
     2341 CAGCCACCAG CCCAGGCAGC CTGTCCTCCA GCAGTGGGCA CGGCTCGGGC TCCTACCCGC 
     2401 TGCCACCTGG CTCCCACCCG CACCTGCCTC ATCCACTGCA TCACCCCCAA GGCCAGCGTC 
     2461 CGTCCAGCGG TAGTGCCTCC GCCTCGGGCT CCCCCAGCGA CCCGGGTTTC ATGTCCCTTG 
     2521 ACGAGTATGG CTCCAGCCCT GGCGACCTGA GAGCCTTCAG TAGCCACAGG AGCAACACAC 
     2581 CCGAGTCAAT AGCGGAGACC CCGCCAGCCA GAGATGGCAG TGGGGGCGAA CTCTATGGGT 
     2641 ACATGAGCAT GGATAGACCC CTGAGCCACT GTGGCCGCCC TTACCGTAGG GTCTCAGGGG 
     2701 ATGGGGCCCA GGACCTGGAT AGAGGACTGA GGAAGAGGAC TTATTCCCTA ACCACGCCTG 
     2761 CCAGGCAGCG GCAGGTACCT CAGCCTTCCT CTGCCTCTCT AGATGAATAC ACTCTCATGA 
     2821 GGGCCACCTT CTCTGGTAGT TCAGGTCGCC TCTGCCCATC CTTCCCTGCG TCCTCTCCCA 
     2881 AAGTGGCCTA CAACCCTTAC CCAGAGGACT ATGGAGACAT TGAGATTGGT TCTCACAAGA 
     2941 GTTCCAGCAG TAACCTGGGG GCAGATGATG GCTACATGCC CATGACCCCT GGGGCAGCCC 
     3001 TTAGGAGTGG TGGTCCCAAT AGCTGCAAGA GCGATGACTA CATGCCCATG AGCCCCACAA 
     3061 GCGTGTCTGC TCCCAAGCAG ATCCTGCAGC CACGCTTGGC AGCGGCCTTG CCCCCTTCCG 
     3121 GAGCAGCCGT GCCAGCACCC CCTTCAGGGG TGGGCAGGAC CTTCCCAGTA AACGGAGGTG 
     3181 GCTACAAAGC CAGCTCCCCA GCGGAGAGCT CCCCAGAAGA CAGTGGGTAC ATGCGAATGT 
     3241 GGTGTGGCTC CAAGCTGTCT ATGGAGAACC CAGACCCTAA GCTACTCCCC AACGGGGACT 
     3301 ACCTCAACAT GTCCCCCAGC GAGGCAGGCA CTGCAGGGAC CCCACCTGAC TTCTCAGCAG 
     3361 CTTTGCGTGG AGGCAGTGAA GGCCTCAAAG GCATCCCGGG CCACTGCTAC AGCTCTTTGC 
     3421 CCCGCTCTTA TAAGGCTCCC TGTTCCTGCA GCGGAGACAA TGACCAGTAT GTGCTCATGA 
     3481 GCTCCCCTGT GGGCCGGATC TTGGAAGAGG AGAGACTGGA GCCCCAGGCC ACCCCAGGGG 
     3541 CTGGCACCTT TGGGGCAGCT GGTGGTAGTC ATACCCAGCC TCATCACTCA GCAGTGCCTT 
     3601 CCTCCATGAG GCCGAGTGCC ATCGGTGGCC GCCCTGAGGG CTTCCTGGGC CAGCGATGTC 
     3661 GGGCAGTGCG GCCTACACGC CTATCGCTAG AGGGACTGCA GACCCTTCCC AGCATGCAAG 
     3721 AGTACCCTCT ACCCACAGAG CCCAAGAGCC CTGGCGAGTA CATCAACATT GACTTTGGTG 
     3781 AGGCAGGTAC CCGTCTGTCT CCGCCTGCCC CCCCACTACT GGCATCCGCG GCCTCATCTT 
     3841 CTTCACTGCT CTCAGCTAGT AGTCCTGCTT CATCCCTGGG TTCAGGAACC CCAGGCACCA 
     3901 GCAGCGACAG CCGGCAGCGC TCTCCACTCT CTGACTATAT GAACCTGGAC TTCAGTTCTC 
     3961 CCAAGTCCCC CAAGCCTAGC ACCCGCAGTG GGGACACAGT AGGCTCCATG GATGGCCTTC 
     4021 TCTCTCCAGA GGCTTCATCC CCATACCCAC CACTGCCCCC ACGTCCTTCC ACTTCCCCTT 
     4081 CCTCCTTACA GCAGCCTCTG CCACCTGCCC CGGGAGACCT ATACCGCCTG CCTCCAGCAT 
     4141 CAGCTGCCAC TTCCCAGGGT CCCACTGCTG GCTCCTCAAT GTCCTCCGAG CCTGGGGATA 
     4201 ATGGTGACTA TACCGAGATG GCCTTTGGTG TGGCTGCAAC CCCGCCACAA CCTATCGTGG 
     4261 CACCTCCAAA GCCAGAAGGT GCCCGAGTGG CCAGTCCCAC ATCGGGCTTG AAGCGGCTAA 
     4321 GTCTCATGGA TCAGGTATCT GGGGTGGAGG CTTTCCTTCA AGTCAGCCAG CCCCCTGACC 
     4381 CCCACCGGGG TGCTAAGGTC ATCCGTGCAG ACCCACAGGG GGGACGTCGT CGCCACAGTT 
     4441 CAGAGACCTT TTCCTCTACC ACCACCGTCA CCCCAGTGTC CCCATCCTTT GCCCACAATT 
     4501 CCAAGCGCCA CAATTCGGCC TCTGTGGAAA ATGTCTCACT CAGGAAAAGC AGTGAAGGCA 
     4561 GCAGTACCCT GGGAGGAGGT GATGAGCCGC CCACATCCCC AGGACAGGCA CAGCCCTTGG 
     4621 TGGCTGTGCC CCCAGTGCCA CAGGCTAGGC CGTGGAACCC CGGTCAGCCC GGAGCTTTGA 
     4681 TTGGCTGTCC TGGAGGCAGC AGTTCTCCCA TGCGCAGAGA GACCTCCGTG GGTTTCCAGA 
     4741 ACGGCCTCAA CTATATCGCC ATCGATGTGA GAGGCGAGCA GGGGTCCTTG GCGCAGTCTC 
     4801 AGCCGCAGCC AGGAGACAAG AACTCCTGGA GCCGGACCCG TAGCCTTGGG GGGCTCCTCG 
     4861 GCACCGTCGG AGGCTCTGGC GCCAGCGGAG TGTGTGGGGG TCCAGGCACT GGAGCTTTGC 
     4921 CCTCTGCCAG CACCTATGCA AGCATCGACT TCCTGTCCCA TCACTTGAAG GAAGCCACAG 
     4981 TCGTGAAAGA GACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     5041 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     5101 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     5161 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     5221 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     5281 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     5341 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     5401 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     5461 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     5521 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     5581 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     5641 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     5701 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     5761 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     5821 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     5881 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     5941 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     6001 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     6061 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     6121 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     6181 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     6241 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     6301 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     6361 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     6421 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     6481 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     6541 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     6601 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     6661 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     6721 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     6781 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     6841 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     6901 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     6961 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     7021 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     7081 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     7141 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     7201 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     7261 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     7321 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     7381 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     7441 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     7501 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     7561 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     7621 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     7681 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     7741 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     7801 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     7861 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     7921 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     7981 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     8041 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     8101 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     8161 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     8221 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     8281 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     8341 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     8401 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     8461 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     8521 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     8581 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     8641 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     8701 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     8761 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     8821 TAACAAAAAT TTAACGCGAA TTTT
//