LOCUS       MR227147                7716 bp    DNA     circular  SYN 
DEFINITION  Hk1 (Myc-DDK-tagged) - Mouse hexokinase 1 (Hk1), nuclear gene encoding mitochondrial protein, transcript variant 2
ACCESSION   NM_010438
VERSION     NM_010438.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3873..3964 
                     /label="Myc-DDK"  
     misc_feature    3994..4579 
                     /label="PolyA signal"  
     misc_feature    4051..4070 
                     /label="Restriction Enzyme"  
     misc_feature    4855..5527 
                     /label="ColE1"  
     misc_feature    5957..6751 
                     /label="Kan/Neo"  
     misc_feature    6786..7088 
                     /label="SV40 ori"  
     misc_feature    7174..7651 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3855..3861 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3855 
                     /label="Hk1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGACAGAAC TGCCAGCGAG GACAGGCTGT AGATGTTGAA CCAAAAATAC 
     1081 GACCCCCTCT CACAGAGGAA AAGATTGATA AGTATCTGTA TGCCATGCGG CTCTCTGATG 
     1141 AAATTCTGAT AGATATCCTG ACACGCTTCA AGAAAGAGAT GAAGAATGGC CTCTCCCGGG 
     1201 ATTATAACCC AACGGCCTCC GTCAAGATGC TGCCAACCTT TGTCCGGTCC ATTCCGGACG 
     1261 GCTCAGAAAA GGGGGATTTC ATTGCACTGG ATCTCGGCGG GTCTTCCTTT CGAATCCTGC 
     1321 GGGTGCAGGT GAACCACGAG AAGAGTCAGA ACGTCAGCAT GGAGTCTGAG GTCTACGACA 
     1381 CCCCAGAGAA CATCGTGCAC GGCAGTGGAA GCCAGCTTTT TGATCACGTC GCTGAATGCC 
     1441 TCGGAGACTT CATGGAGAAA AGGAAGATCA AGGACAAGAA ATTACCCGTG GGATTCACGT 
     1501 TTTCCTTCCC GTGCCGACAA TCCAAAATAG ACGAGGCCGT ACTGATCACG TGGACAAAGC 
     1561 GGTTCAAAGC CAGTGGCGTG GAAGGGGCGG ATGTGGTCAA GCTGCTGAAT AAAGCCATTA 
     1621 AGAAGCGAGG GGACTATGAC GCTAACATTG TAGCTGTGGT GAATGACACA GTGGGGACCA 
     1681 TGATGACCTG CGGCTACGAT GACCAACAGT GTGAAGTCGG CCTGATCATT GGCACTGGCA 
     1741 CCAATGCTTG CTACATGGAG GAACTGCGAC ACATCGACCT GGTGGAAGGC GATGAGGGGA 
     1801 GGATGTGTAT TAACACGGAA TGGGGAGCCT TTGGGGATGA TGGGTCCCTG GAAGACATTC 
     1861 GAACAGAGTT TGACAGAGAG TTAGACCGGG GATCCCTCAA CCCTGGGAAA CAGCTGTTCG 
     1921 AGAAGATGGT GAGCGGCATG TACATGGGGG AGCTGGTCCG GCTGATCCTG GTGAAGATGG 
     1981 CCAAGGAAAG CCTCTTATTT GAAGGGCGCA TTACTCCAGA GCTGCTCACG AGGGGCAAGT 
     2041 TCACCACTAG CGACGTAGCC GCCATTGAAA CGGGATGGGA ACTCTCCCCT GACCGCAGGT 
     2101 GGTACCAGGC ATATATGAGG TGCACACAAG ACACCCACAG GGATAAGGAA GGCGTTCAAA 
     2161 ATGCCAAGGA AATCTTGACC CGCCTGGGAG TGGAGCCGTC TCACGATGAC TGCGTATCGG 
     2221 TCCAGCACGT ATGCACGATC GTCTCCTTCC GATCAGCCAA CCTGGTGGCT GCCACGCTCG 
     2281 GTGCCATCTT GAACCGCCTG CGGGACAATA AGGGCACGCC CAGGCTGCGG ACCACAGTTG 
     2341 GCGTAGACGG TTCTCTCTAC AAGATGCACC CACAGTATTC CCGGCGGTTC CACAAGACCC 
     2401 TGAGGCGCCT GGTGCCTGAC TCGGACGTCC GGTTCCTCCT CTCGGAGAGT GGCAGTGGCA 
     2461 AGGGAGCCGC CATGGTGACC GCTGTGGCCT ACCGCCTGGC CGAGCAGCAC CGGCAGATTG 
     2521 AGGAAACCCT GTCCCACTTC CGCCTCAGCA AGCAGGCACT GATGGAGGTG AAGAAGAAGC 
     2581 TGCGGTCAGA GATGGAAATG GGGCTGAGAA AGGAGACCAA CAGCAGAGCT ACGGTCAAAA 
     2641 TGCTGCCTTC TTATGTTCGG AGCATCCCAG ATGGGACTGA GCATGGTGAC TTCCTGGCCT 
     2701 TGGATCTCGG AGGAACGAAT TTCCGAGTCC TACTGGTAAA GATCCGTAGT GGGAAAAAGA 
     2761 GAACAGTGGA GATGCACAAC AAGATCTACT CCATTCCCCT GGAAATCATG CAGGGCACCG 
     2821 GGGATGAGCT GTTTGATCAC ATTGTCTCCT GCATCTCCGA CTTCCTGGAC TACATGGGGA 
     2881 TCAAAGGCCC CCGGATGCCT CTGGGCTTCA CCTTCTCGTT TCCCTGCAAG CAGACGAGCC 
     2941 TAGATTGCGG AATCTTGATC ACGTGGACAA AGGGATTCAA AGCCACCGAC TGTGTGGGTC 
     3001 ACGATGTAGC CACTTTACTG AGGGATGCTG TAAAAAGGAG AGAGGAATTT GACCTGGATG 
     3061 TGGTGGCTGT GGTCAACGAC ACCGTGGGCA CCATGATGAC TTGTGCTTAT GAAGAACCTT 
     3121 CTTGTGAGAT TGGACTCATC GTGGGGACTG GCAGCAATGC CTGCTACATG GAGGAGATGA 
     3181 AAAACGTGGA GATGGTGGAG GGGAACCAGG GCCAGATGTG CATCAATATG GAATGGGGTG 
     3241 CCTTTGGTGA CAACGGCTGT CTGGACGACA TCAGAACAGA CTTCGACAAA GTGGTGGACG 
     3301 AATATTCTCT AAACAGTGGG AAACAAAGGT TTGAGAAGAT GATCAGTGGA ATGTACCTGG 
     3361 GTGAGATCGT CCGTAACATC CTGATTGACT TCACCAAGAA AGGCTTCCTC TTCCGGGGAC 
     3421 AGATCTCTGA GCCACTCAAG ACCCGAGGCA TCTTCGAGAC CAAGTTTCTC TCTCAGATCG 
     3481 AGAGTGACCG ATTAGCGCTG CTCCAGGTGC GGGCCATCCT TCAGCAGCTG GGTCTAAACA 
     3541 GCACGTGCGA CGACAGTATC CTGGTCAAGA CCGTGTGTGG GGTGGTGTCC AAGCGGGCGG 
     3601 CCCAGCTGTG TGGTGCCGGC ATGGCCGCCG TGGTGGAAAA GATCCGAGAG AACAGAGGCC 
     3661 TAGACCACCT GAATGTAACC GTGGGCGTGG ACGGGACGCT CTACAAACTC CATCCACACT 
     3721 TCTCCAGAAT CATGCACCAA ACAGTGAAGG AACTGTCACC AAAGTGTACC GTGTCCTTCC 
     3781 TCCTGTCTGA AGACGGCAGC GGCAAGGGGG CCGCCCTTAT CACAGCTGTG GGCGTGCGGC 
     3841 TCAGAGGAGA CCCTACGAAC GCCACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     3901 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     3961 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     4021 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     4081 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     4141 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     4201 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     4261 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     4321 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     4381 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     4441 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     4501 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     4561 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     4621 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     4681 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     4741 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     4801 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     4861 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     4921 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     4981 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     5041 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     5101 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     5161 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     5221 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     5281 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     5341 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     5401 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     5461 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     5521 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     5581 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     5641 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     5701 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     5761 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     5821 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     5881 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     5941 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     6001 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     6061 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     6121 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     6181 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     6241 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     6301 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     6361 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     6421 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     6481 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     6541 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     6601 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     6661 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     6721 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     6781 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     6841 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     6901 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     6961 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7021 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7081 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     7141 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     7201 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     7261 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     7321 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     7381 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     7441 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     7501 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     7561 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     7621 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     7681 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//