LOCUS       MR227138                8841 bp    DNA     circular  SYN 
DEFINITION  Tcof1 (Myc-DDK-tagged) - Mouse Treacher Collins Franceschetti syndrome 1, homolog (Tcof1), transcript variant 2
ACCESSION   NM_011552
VERSION     NM_011552.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4998..5089 
                     /label="Myc-DDK"  
     misc_feature    5119..5704 
                     /label="PolyA signal"  
     misc_feature    5176..5195 
                     /label="Restriction Enzyme"  
     misc_feature    5980..6652 
                     /label="ColE1"  
     misc_feature    7082..7876 
                     /label="Kan/Neo"  
     misc_feature    7911..8213 
                     /label="SV40 ori"  
     misc_feature    8299..8776 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4980..4986 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4980 
                     /label="Tcof1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCGAGGCC AGGAAGCGGC GGGAGCTGCT TCCCCTCATT TACCATCATC 
     1081 TGTTGCAAGC AGGCTACGTC CGCGCGGCGC GGGAAGTAAA GGAGCAGAGC GGCCAGAAGA 
     1141 GTTTCCTGAC TCAGCCCGTC ACCCTTCTGG ACATCTATAC ACACTGGCAA CAGACCTCAG 
     1201 AGCTTGGCCA GAAGCAGAAG GCAGAGGATG ATGAGACCCT TCAGGCTAAG AAGTCTCGAG 
     1261 TGTCGGATCC TGTTAGCAGC TCAGAGAGCT CGGATCAGGA GAAGGAAGAG GAGGCAGCAA 
     1321 CCGAAAGGGC CAAAGCCACC CCAAGACCGA CACCTGTCAA TTCTGCAACC GCAGCTTTGC 
     1381 CATCAAAAGT AAAAGAAAAG GGAAAGACCA AGACAGCCAA CAAGACGGTG AACTCTGTGT 
     1441 CGCACCCTGG GTCCGGAAAG ACGGTGGTCC ACCTGCTCTC TGGGAAGTCA CCCAAAAAGT 
     1501 CAGCAGAGCC CTTGGCAAAC ACTGTCTTGG CCTCAGAAAC TGAGGAGGAG GGCAATGCCC 
     1561 AAGCCCTCGG ACCCACTGCC AAGTCTGGAA CGGTGTCAGC GGGCCAAGGC AGCAGTTCCA 
     1621 GTGAAGATTC CTCCATCTCA AGCGATGAGA CAGATGTCGA GGTGAAATCT CCAGCAAAAC 
     1681 CAGCCCAGGC CAAAGCTTCA GCAGCCCCTG CCAAGGATCC TCCAGCAAGA ACAGCCCCAG 
     1741 GCCCTACCAA GTTAGGGAAT GTGGCGCCCA CACCTGCTAA ACCAGCCAGG GCGGCAGCGG 
     1801 CAGCTGCTGC TGCTGCTGTG GCTGCTGCTG CTGCTGCAGC AGCAGAAGAG TCTGAGAGCA 
     1861 GTGAGGAGGA CTCAGACAGT GAGGACGAGG CCCCTGCTGG TCTGCCCAGC CAGGTAAAAG 
     1921 CCTCTGGAAA AGGTCCCCAT GTCAGAGCCG ACTCGGTGTC TGCCAAGGGG ATCTCTGGGA 
     1981 AAGGGCCCAT CTTAGCAACC CCAGGGAAGA CTGGGCCTGC AGCCACCCAG GCCAAGGCAG 
     2041 AAAGGCCAGA GAAGGACTCG GAAACCAGCA GTGAGGACGA TTCTGATAGT GAGGATGAAA 
     2101 TGCCAGTCAC TGTGAATACT CCTCAGGCAA GGACTTCTGG GAAGAGCCCT CGGGCCAGAG 
     2161 GTACCTCAGC CCCCGCCAAG GAGTCATCCC AGAAAGGGGC TCCTGCAGTC ACCCCTGGAA 
     2221 AGGCAAGGCC TGTGGCAGCC CAGGCAGGGA AACCAGAAGC CAAGAGCAGT GAGGAGTCAG 
     2281 AGAGTGACAG TGGGGAGACA CCAGCTGCTG CGACTCTGAC CACGAGTCCT GCCAAGGTGA 
     2341 AACCTTTGGG GAAGAGCTCC CAGGTCAGAC CTGTTTCCAC CGTCACCCCG GGGTCATCGG 
     2401 GAAAAGGTGC CAACCTGCCC TGCCCTGGGA AGGTGGGGTC AGCAGCTCTC AGGGTCCAAA 
     2461 TGGTAAAGAA AGAAGATGTC TCGGAGAGCA GCAGTGCAGA GCTGGACAGT GACGGGCCTG 
     2521 GGAGCCCAGC CAAGGCAAAG GCCTCCCTTG CGCTCCCTCA GAAGGTGAGG CCTGTGGCCA 
     2581 CCCAGGTCAA GACTGACAGG GGCAAAGGCC ACTCAGGGAG CAGTGAGGAG TCATCTGACA 
     2641 GCGAAGAGGA GGCAGCACCA GCAGCCTCTG CTGCTCAGGC TAAGCCAGCT CTGGAAAAGC 
     2701 AGATGAAAGC TTCCTCTAGG AAAGGCACGC CTGCATCCGC AACAGGAGCG AGCACCTCGT 
     2761 CCCATTGTAA GGCAGGAGCG GTGACCTCTT CAGCCAGCCT GTCATCCCCA GCTCTGGCCA 
     2821 AGGGCACCCA GAGGTCAGAT GTGGACTCTT CCAGTGAGTC TGAGTCAGAA GGAGCTGCTC 
     2881 CCAGCACCCC CAGGGTACAG GGGAAGTCTG GGGGCAAGGG CCTCCAAGGG AAAGCTGCCT 
     2941 TGGGGCAAGG GGTGGCCCCA GTGCACACTC AGAAGACAGG GCCTTCGGTC AAAGCTATGG 
     3001 CTCAGGAAGA CTCAGAGAGC CTCGAGGAGG ACTCCAGCAG TGAGGAAGAG GATGAGACCC 
     3061 CAGCACAGGC CACGCCCTTG GGGAGACTTC CTCAGGCCAA AGCCAACCCA CCTCCCACTA 
     3121 AGACACCTCC AGCGTCTGCA TCTGGAAAAG CTGTGGCTGC TCCAACCAAG GGAAAACCAC 
     3181 CTGTTCCGAA CAGCACCGTC TCTGCAAGGG GCCAGCGGTC TGTGCCAGCC GCGGGAAAAG 
     3241 CAGGGGCCCC AGCAACCCAA GCCCAGAAGG GTCCCGTGGC TGGCACAGGG GAGGACTCAG 
     3301 AGAGCAGCAG TAAAGAGGAG TCTGACAGTG AAGAAGAGAC GCCAGCCCAG ATAAAACCTG 
     3361 TGGGGAAGAC CTCTCAGGTC AGAGCTGCCT CAGCCCCTGC CAAGGAGTCT CCTAAAAAAG 
     3421 GAGCCCATCC AGGAACCCCC GGCAAGACGG GATCTTCAGC TACCCAGGCC CAGCCAGGGA 
     3481 AGACAGAGGA CTCAGACAGC AGTAGTGAAG AGTCTGACAG TGACACAGAG ATGCCATCAG 
     3541 CCCAGGCGAT TAAATCCCCT CCAGTTTCTG TCAACCGTAA CAGTAGTCCA GCTGTCCCAG 
     3601 CTCCTACCCC AGAAGGAGTC CAGGCTGTGA ACACCACAAA GAAGGCCTCA GGCACCACTG 
     3661 CCCAGAGCTC CTCCTCTGAG AGTGAGGACG GGGACGAGGA CTTGATTCCT GCCACACAAC 
     3721 CCTCCACCTA TGCTCTCAGA ACCAGTGTGA CGACGCCCGC AGCCCTCTCA CGAGCAGCTT 
     3781 CCCAACCCAG CAAGAGTGAG CAGTCTAGCC GGATGCCAAA AGGCAAGAAA GCAAAGGCGG 
     3841 CGGCGTCCGC TCAGACCAGC AGTGCCGTGG AAACACTCCC CATGATGCCT CCCCAGAGCG 
     3901 CACCCATCCA GCCCAAAGCC ACCAACAAGC TCGGGAAATC CAAGCTCCCT GAGAAGCAGC 
     3961 AGCTTGCCCC AGGCTACCCC AAGGCCCCCA GGAGCTCGGA GGACAGCAGT GACACTTCTT 
     4021 CCGAGGACGA GGAGGATGCC AAGAGACCCC AGATGCCCAA GTCGGCCCAC AGGCTGGATC 
     4081 CAGACCCTTC CCAGAAGGAA ACTGTGGTAG AGGAGACCCC TACAGAATCC AGCGAAGATG 
     4141 AGATGGTGGC CCCCTCACAG TCTCTCCTCT CAGGTTACAT GACTCCCGGC TTGACTGTGG 
     4201 CTAATTCCCA GGCTTCAAAA GCTACTCCTA GGCCAGACTC CAACTCCTTA GCTTCTTCTG 
     4261 CCCCGGCCAC CAAAGACAAC CCGGATGGCA AGCAGAAGTC AAAATCCCAA CACGCAGCAG 
     4321 ACACCGCACT CCCTAAAACC GGTAGGAAAG AGGCCTCCTC GGGCTCCACG CCTCAGAAGC 
     4381 CCAAGAAGCT CAAGAAGAGC ACCTCAAGCT CGCCGGCCCC CACACAGACA CTGCCGAACA 
     4441 GCATCACCCA GCGCCTCCTG GAGCAGGCCT GGCCCCTGAG CGAGGCGCAG GTGCAGGCCT 
     4501 CTGTGGTGAA GGTCCTGACA GAGCTTCTGG AGCAGGAACG GCTGAAGGCC ACAGAGGCCA 
     4561 TCAAGGAGAG TGGGAAGAAG AGCCAGAAGC GGAAGTTATC AGGGGACCTA GAAGCTGGGG 
     4621 CCCCAAAGAA CAAGAAGAAG AAGGAGCAGC CAGTGCCCAG GGCAAGCGCT GTTTCCCCAG 
     4681 AAAAGGCTCC CATGACTTCC AAGGCAAAAT CAAAGCTTGA CAAAGGGAGT GCTGGTGGCA 
     4741 AGGGGAAGGG GTCTCCTGGC CCCCAAGGAG CCAAGGAGAA GCCCGACGGC GAGTTGCTGG 
     4801 GGATAAAGCT TGAGAGTGGC GAGCAGAGCG ACCCGAAGAG CAAGTCGAAA AAGAAGAAAT 
     4861 CCCTCAAGAA AAAAAAAGAC AAGGAGAAAA AGGAAAAGAA GAAAGGAAAA AAGTCCCTGG 
     4921 CCAAAGACTC TGCCTCGCCG ATCCAGAAGA AGAAAAAGAA GAAGAAGAAG TCAGCCGAGC 
     4981 CTGCCGTGAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     5041 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     5101 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     5161 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     5221 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     5281 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     5341 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     5401 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     5461 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     5521 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     5581 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     5641 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     5701 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     5761 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     5821 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     5881 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     5941 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     6001 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     6061 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     6121 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     6181 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     6241 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     6301 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     6361 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     6421 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     6481 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     6541 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     6601 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     6661 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     6721 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     6781 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     6841 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     6901 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     6961 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     7021 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     7081 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     7141 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     7201 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     7261 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     7321 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     7381 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     7441 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     7501 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     7561 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     7621 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     7681 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     7741 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     7801 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     7861 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     7921 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     7981 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     8041 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     8101 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     8161 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     8221 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     8281 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     8341 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     8401 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     8461 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     8521 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     8581 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     8641 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     8701 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     8761 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     8821 CAAAAATTTA ACGCGAATTT T
//