LOCUS       MR227135                9273 bp    DNA     circular  SYN 
DEFINITION  Grin2a (Myc-DDK-tagged) - Mouse glutamate receptor, ionotropic, NMDA2A (epsilon 1) (Grin2a)
ACCESSION   NM_008170
VERSION     NM_008170.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5430..5521 
                     /label="Myc-DDK"  
     misc_feature    5551..6136 
                     /label="PolyA signal"  
     misc_feature    5608..5627 
                     /label="Restriction Enzyme"  
     misc_feature    6412..7084 
                     /label="ColE1"  
     misc_feature    7514..8308 
                     /label="Kan/Neo"  
     misc_feature    8343..8645 
                     /label="SV40 ori"  
     misc_feature    8731..9208 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5412..5418 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5412 
                     /label="Grin2a"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGCAGACTG GGCTACTGGA CCTTGCTGGT ATTGCCGGCC CTTCTGGTCT 
     1081 GGCACGGTCC GGCGCAGAAC GCGGCGGCGG AGAAGGGTAC TCCAGCGCTG AACATTGCGG 
     1141 TGCTGCTGGG TCACAGCCAC GACGTGACAG AACGCGAACT TCGAAATCTG TGGGGCCCGG 
     1201 AGCAGGCAAC CGGCTTGCCC CTGGATGTGA ACGTGGTGGC GTTATTGATG AACCGCACTG 
     1261 ACCCTAAGAG CCTCATCACG CATGTGTGCG ACCTCATGTC CGGGGCGCGC ATCCATGGCT 
     1321 TGGTGTTTGG AGATGATACG GACCAAGAGG CTGTGGCTCA GATGCTGGAT TTTATCTCCT 
     1381 CACAGACTTT CATCCCCATC TTGGGCATTC ATGGGGGTGC ATCTATGATC ATGGCTGACA 
     1441 AGGATCCGAC ATCCACGTTC TTCCAGTTTG GAGCTTCCAT CCAGCAGCAA GCCACGGTCA 
     1501 TGCTGAAGAT CATGCAGGAC TATGACTGGC ATGTCTTCTC CTTGGTCACC ACCATCTTCC 
     1561 CTGGCTACAG AGACTTCATC AGCTTCATCA AGACAACAGT GGACAACAGC TTTGTGGGCT 
     1621 GGGATATGCA GAACGTGATC ACACTGGACA CCTCTTTTGA GGACGCCAAG ACACAGGTCC 
     1681 AGCTGAAGAA GATCCACTCC TCTGTCATCC TGCTCTACTG CTCCAAGGAT GAGGCTGTCC 
     1741 TCATCCTGAG CGAGGCTCGC TCTCTTGGCC TCACCGGCTA CGATTTCTTC TGGATTGTCC 
     1801 CCAGTTTGGT CTCCGGGAAC ACAGAGCTCA TCCCCAAAGA GTTTCCATCG GGTCTCATTT 
     1861 CAGTCTCTTA CGACGACTGG GACTACAGTC TGGAGGCAAG AGTGAGAGAC GGTCTTGGGA 
     1921 TCTTAACCAC TGCCGCATCT TCCATGTTGG AGAAATTCTC CTACATTCCC GAGGCCAAGG 
     1981 CCAGCTGCTA CGGGCAGACA GAGAAGCCGG AGACCCCGCT ACACACTCTG CACCAATTTA 
     2041 TGGTCAATGT GACTTGGGAT GGCAAGGACT TGTCCTTCAC TGAGGAAGGC TATCAGGTGC 
     2101 ACCCCAGGCT TGTGGTGATC GTGCTGAATA AGGACCGGGA ATGGGAAAAG GTGGGCAAGT 
     2161 GGGAGAATCA GACTCTGAGC CTGCGGCATG CTGTGTGGCC AAGGTATAAG TCCTTTTCTG 
     2221 ACTGTGAGCC AGATGACAAC CACCTCAGCA TTGTCACCTT GGAGGAAGCC CCCTTCGTCA 
     2281 TCGTAGAAGA CATAGACCCA CTGACTGAGA CCTGCGTCAG GAACACGGTA CCCTGTCGGA 
     2341 AGTTTGTCAA GATCAACAAT TCAACCAACG AAGGGATGAA TGTGAAGAAG TGCTGCAAGG 
     2401 GGTTCTGCAT CGACATCCTT AAGAAACTGT CCAGAACTGT AAAGTTCACC TATGACCTCT 
     2461 ACCTGGTGAC CAATGGGAAG CATGGGAAAA AGGTTAACAA TGTGTGGAAT GGAATGATAG 
     2521 GCGAAGTGGT CTATCAACGA GCAGTTATGG CCGTGGGCTC CCTCACCATC AATGAGGAGC 
     2581 GTTCAGAAGT GGTGGACTTC TCCGTGCCCT TTGTGGAGAC AGGAATCAGT GTCATGGTCT 
     2641 CCAGGAGTAA TGGCACTGTT TCCCCTTCTG CTTTCCTTGA ACCCTTCAGC GCCTCTGTCT 
     2701 GGGTGATGAT GTTCGTGATG CTGCTCATTG TCTCTGCCAT TGCTGTCTTC GTTTTTGAAT 
     2761 ACTTCAGTCC TGTTGGATAC AACAGAAACT TAGCCAAAGG GAAAGCTCCC CATGGGCCTT 
     2821 CTTTTACCAT TGGAAAAGCT ATATGGCTCC TCTGGGGCCT GGTCTTCAAC AATTCTGTGC 
     2881 CCGTCCAGAA TCCTAAAGGC ACAACCAGCA AGATAATGGT ATCAGTGTGG GCCTTCTTTG 
     2941 CCGTCATCTT CCTTGCAAGT TACACAGCCA ACCTGGCTGC CTTCATGATC CAGGAGGAGT 
     3001 TTGTGGACCA AGTGACTGGC CTCAGTGACA AGAAGTTCCA GAGACCTCAT GACTATTCTC 
     3061 CGCCTTTCCG ATTTGGGACA GTACCCAATG GAAGTACAGA AAGGAATATT CGTAACAACT 
     3121 ACCCCTATAT GCACCAGTAC ATGACCAAAT TCAACCAGAG GGGCGTAGAG GATGCCTTGG 
     3181 TCAGCTTGAA AACTGGGAAG TTGGACGCTT TCATCTATGA CGCAGCTGTC TTGAACTACA 
     3241 AGGCCGGGAG GGATGAAGGC TGTAAACTGG TGACCATTGG GAGCGGGTAC ATCTTTGCCA 
     3301 CCACAGGCTA TGGAATTGCT CTGCAGAAGG GCTCACCCTG GAAGAGGCAG ATTGACCTCG 
     3361 CTCTGCTCCA GTTTGTTGGT GACGGTGAGA TGGAAGAGCT GGAGACACTG TGGCTTACGG 
     3421 GCATCTGCCA CAACGAGAAG AATGAGGTGA TGAGCAGCCA GCTGGACATC GACAACATGG 
     3481 CAGGAGTTTT CTACATGCTG GCTGCAGCTA TGGCCCTCAG CCTCATCACC TTCATCTGGG 
     3541 AGCACCTCTT CTACTGGAAG CTGCGCTTCT GCTTCACAGG CGTGTGTTCT GACCGGCCCG 
     3601 GGCTGCTCTT CTCCATCAGC AGGGGCATCT ACAGTTGCAT CCATGGAGTG CACATTGAAG 
     3661 AAAAGAAGAA GTCTCCAGAC TTCAATCTGA CTGGGTCACA GAGCAACATG CTAAAGCTTC 
     3721 TCCGCTCAGC TAAAAACATC TCCAACATGT CCAACATGAA CTCCTCGCGA ATGGACTCAC 
     3781 CCAAAAGAGC TGCTGACTTC ATCCAAAGAG GCTCACTTAT TGTGGACATG GTTTCAGACA 
     3841 AGGGAAATTT GATATACTCA GATAACAGGT CCTTTCAAGG GAAGGACAGT ATATTTGGAG 
     3901 AAAACATGAA TGAACTGCAA ACATTTGTGG CCAACAGGCA CAAGGATAGT CTCAGTAACT 
     3961 ATGTGTTTCA GGGACAGCAT CCTCTCACTC TCAATGAGTC CAACCCCAAC ACAGTGGAGG 
     4021 TGGCTGTCAG CACTGAATCC AAAGGGAACT CCCGACCCCG GCAGCTTTGG AAGAAATCCA 
     4081 TGGAGTCTCT ACGCCAGGAT TCTCTAAACC AGAACCCAGT CTCCCAGAGG GATGAGAAGA 
     4141 CTGCAGAGAA TAGGACCCAC TCCCTAAAGA GTCCTAGGTA TCTTCCAGAA GAGGTAGCCC 
     4201 ATTCTGACAT TTCTGAAACC TCAAGCCGGG CCACATGCCA CAGGGAGCCA GATAATAATA 
     4261 AGAACCACAA GACCAAGGAT AACTTCAAAA GGTCAATGGC CTCTAAATAC CCCAAGGACT 
     4321 GTAGTGAGGT TGAACGTACC TACGTGAAAA CCAAAGCAAG TTCTCCCAGG GATAAGATCT 
     4381 ACACCATCGA TGGTGAGAAG GAGCCCAGCT TCCACTTAGA TCCTCCACAG TTCATTGAAA 
     4441 ACATAGTCTT GCCTGAGAAT GTGGACTTCC CAGATACCTA CCAAGATCAC AATGAGAATT 
     4501 TCCGCAAGGG GGACTCCACA CTGCCCATGA ACAGGAACCC ACTACACAAT GAAGATGGGC 
     4561 TTCCCAACAA TGACCAGTAT AAACTCTATG CCAAGCACTT TACCTTGAAA GACAAGGGTT 
     4621 CCCCACATAG TGAGGGCAGT GATCGATATC GGCAGAACTC CACGCATTGC AGAAGCTGCC 
     4681 TCTCAAACCT GCCCACCTAC TCAGGCCACT TTACCATGAG ATCTCCTTTC AAGTGTGATG 
     4741 CCTGTCTGCG GATGGGGAAC CTCTATGACA TTGATGAAGA CCAGATGCTT CAGGAGACAG 
     4801 GCAACCCAGC TACTCGTGAG GAGGCCTACC AGCAGGACTG GTCACAGAAC AACGCCCTCC 
     4861 AGTTCCAGAA GAACAAGCTA AAGATTAATC GACAGCACTC CTATGATAAC ATTCTCGACA 
     4921 AACCCAGGGA GATAGACCTT AGCAGGCCCT CTCGTAGCAT AAGCCTCAAG GACAGGGAAA 
     4981 GGCTACTGGA GGGCAACTTA TACGGGAGCC TGTTCAGTGT CCCCTCAAGC AAACTCTTGG 
     5041 GGAACAAAAG CTCCCTTTTC CCCCAAGGTC TGGAGGACAG CAAGAGGAGC AAATCTCTCT 
     5101 TGCCAGACCA TACCTCTGAT AATCCTTTCC TCCACACGTA CGGGGATGAC CAACGCTTAG 
     5161 TTATTGGGAG ATGTCCCTCG GACCCTTACA AACACTCATT GCCATCACAG GCAGTAAATG 
     5221 ACAGCTATCT TCGGTCATCC TTGAGGTCAA CAGCATCATA TTGCTCCAGG GACAGTCGGG 
     5281 GCCACAGTGA TGTGTATATT TCAGAGCATG TTATGCCTTA TGCTGCAAAT AAGAATAACA 
     5341 TGTACTCTAC CCCCAGGGTT TTAAATTCCT GCAGCAATAG ACGTGTGTAC AAGAAAATGC 
     5401 CTAGTATTGA ATCTGATGTC ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     5461 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     5521 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     5581 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     5641 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     5701 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     5761 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     5821 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     5881 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     5941 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     6001 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     6061 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     6121 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     6181 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     6241 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     6301 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     6361 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     6421 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     6481 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     6541 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     6601 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     6661 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     6721 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     6781 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     6841 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     6901 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     6961 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     7021 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     7081 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     7141 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     7201 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     7261 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     7321 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     7381 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     7441 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     7501 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     7561 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     7621 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     7681 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     7741 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     7801 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     7861 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     7921 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     7981 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     8041 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     8101 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     8161 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     8221 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     8281 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     8341 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     8401 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     8461 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     8521 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     8581 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     8641 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     8701 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     8761 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     8821 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     8881 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     8941 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     9001 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     9061 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     9121 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     9181 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     9241 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//