LOCUS       MR227113                8805 bp    DNA     circular  SYN 
DEFINITION  Ttbk1 (Myc-DDK-tagged) - Mouse tau tubulin kinase 1 (Ttbk1). Note: ORF is codon optimized
ACCESSION   NM_001162864
VERSION     NM_001162864.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4962..5053 
                     /label="Myc-DDK"  
     misc_feature    5083..5668 
                     /label="PolyA signal"  
     misc_feature    5140..5159 
                     /label="Restriction Enzyme"  
     misc_feature    5944..6616 
                     /label="ColE1"  
     misc_feature    7046..7840 
                     /label="Kan/Neo"  
     misc_feature    7875..8177 
                     /label="SV40 ori"  
     misc_feature    8263..8740 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4944..4950 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4944 
                     /label="Ttbk1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCAATGCCTT GCAGCTGCTC TGAAGGACGA GACTAACATG TCAGGCGGTG 
     1081 GGGAGCAAGC GGACATTCTG CCTGCGAACT ATGTGGTTAA AGACCGGTGG AAGGTGCTGA 
     1141 AAAAGATCGG AGGAGGCGGA TTCGGGGAGA TCTACGAGGC AATGGATCTG TTGACACGAG 
     1201 AGAATGTAGC CCTTAAGGTG GAGAGTGCCC AGCAGCCGAA GCAGGTCCTG AAGATGGAGG 
     1261 TGGCCGTCCT TAAGAAGCTG CAGGGCAAAG ACCACGTTTG TCGATTCATC GGCTGCGGGA 
     1321 GAAATGAAAA ATTTAATTAC GTGGTCATGC AGCTCCAGGG ACGGAACCTC GCCGATCTTC 
     1381 GAAGATCTCA GCCACGGGGC ACATTTACGC TGTCCACAAC TTTGCGGCTG GGCAAGCAGA 
     1441 TCCTTGAGTC TATTGAGGCC ATTCACAGCG TGGGATTCCT TCACAGAGAT ATCAAGCCGT 
     1501 CTAACTTTGC AATGGGGCGC CTCCCTTCTA CATACCGGAA ATGTTACATG CTTGATTTTG 
     1561 GCCTTGCCAG GCAGTACACC AATACCACCG GGGACGTGCG GCCACCCAGG AACGTGGCAG 
     1621 GATTCCGCGG GACAGTGCGG TATGCCTCTG TTAATGCCCA CAAAAATCGG GAGATGGGGC 
     1681 GCCATGATGA CCTGTGGAGC CTGTTCTATA TGTTGGTGGA ATTCGCGGTG GGACAGCTGC 
     1741 CATGGCGCAA GATCAAGGAC AAGGAGCAGG TGGGCATGAT CAAAGAGAAG TATGAGCATA 
     1801 GGATGTTGCT CAAACACATG CCCAGCGAGT TCCATCTGTT CCTTGACCAC ATCGCAAGCC 
     1861 TCGACTACTT TACAAAGCCT GATTACCAGT TGATTATGTC AGTTTTCGAG AACAGCATGA 
     1921 AGGAACGAGG AATCGCAGAG AATGAGGCTT TCGACTGGGA GAAGGCCGGC ACAGACGCTC 
     1981 TCCTGTCAAC AAGCACCTCT ACCCCCCCTC AGCAGAACAC TAGGCAGACT GCTGCCATGT 
     2041 TTGGAGTGGT CAATGTTACA CCCGTCCCAG GTGACCTTCT CAGGGAGAAC ACTGAAGATG 
     2101 TCCTTCAGGG GGAGCATTTG TCTGATCAGG AAAACGCTCC TCCGATTCTC CCCGGAAGAC 
     2161 CGCCCGAAGG CCTGGGCCCT GGACCCCATC TTGTGCCTCA TCCAGGAGGT CCTGAGGCCG 
     2221 AGGTGTGGGA GGAAACCGAT GTGAATCGGA ACAAGCTTCG AATCAACATC GGAAAAACTC 
     2281 CATGTGTCGA GGAGGAGCAG AGCAGAGGAG TTGGGGTACC CTCCAGTCCA GTGCGAGCAC 
     2341 CTCCGGACAG CCCTACAACG CCTGTGAGGT CCCTTTGCTA TAGGAGAGTG AACAGCCCAG 
     2401 AAAGCGAGCG GCTTTCCACC GCTGCAGACG GTCGGGTTGA GCTTCAGGAA CGGCGATCCA 
     2461 GGATGGACCT GCCTGGTTCA CCATCTAGGC AAGCATGTTC CTCACAGCCA GCTCAGATGC 
     2521 TTTCCGTGGA CACCGGACAT GCTGATAGAC AAGCCAGCGG CCGGATGGAT GTAAGTGCCA 
     2581 GTGTCGAGCA AGAAGCTCTG TCCAACGCAT TCAGGTCTGT GCCTCTGGCA GAGGAGGAGG 
     2641 ACTTCGATTC TAAAGAGTGG GTCATAATCG ACAAGGAAAC CGAATTGAAG GATTTCCCCC 
     2701 CCGGCGCTGA ACCGAGCACT TCCGGCACCA CCGACGAGGA ACCGGAAGAG CTGCGCCCCT 
     2761 TGCCCGAGGA GGGCGAGGAA AGGCGCCGAC TGGGAACAGA ACCTACAGTT CGCCCTCGCG 
     2821 GCCGCGGGAT GCACACGCTT ACTGAGGAGG ATCCTAGGCA AATGCTCCCC CAGCCCGCTC 
     2881 CACCACAGCT GTCCCAGGCT GACGGCCGCA GCGAGACATC TCAACCCCCC ACTCCCGGTT 
     2941 CCCCCAGCCA CTCCCCCCTG CACTCAGGAC CTAGGCCCCG CCGGCGAGAA TCAGATCCGA 
     3001 CAGGACCCCA GAGGCAGGTG TTTAGTGTGG CCCCGCCTTT CGAGGTGAAT GGACTGCCTA 
     3061 GGGCCGTGCC TCTCGCTCTT CCATACCAGG ACTTTAAGAG GGACCTGTCT GACTACAGGG 
     3121 AACGAGCACG CCTGCTGAAT CGCGTACGGC GAGTGGGCTT CAGTCACATG CTGCTGACCG 
     3181 CCCCTCAAGT CCCTCTCGCG CCATTCCAAC CACAGGCGAA TGGTAAAGAG GGCGAGGAGG 
     3241 AGGAGGAAGA AGAGGAAGAG GAAGAGGAGG AAGAGGAGGA AGAGGAGGAG GAGGAAGAGG 
     3301 AGGAGGAGGA GGAAGAAGAG GAGGAAGAGG AGGCAGGAGC ATTGGGTGAG GTTCTTGGGC 
     3361 CCAGAAGCGG TTCTTCCAGC GAGGGCTCCG AGAGGTCAAC CGAGCGCAGC CAGGAAGGAG 
     3421 CACCTAGTAC TCTGCTTGCC GACGATCAGA AGGAGGCAAG GGGGCGCGCC TCTATGGCCG 
     3481 ACGGCGATCT GGAACCAGAG GAGGGTAGCA AAACGCTTGT GCTTGTCAGC CCCGGCGATA 
     3541 TGAAGAAGAG TCCAGTTACC GCTGAGCTGG CACCCGATCC AGACTTGGGC ACCTTGGCTG 
     3601 CTCTTACCCC CCAGCACGAG AGGCCCCAGC CCACCGGTTC CCAATTGGAC GTTTCTGAGC 
     3661 CAGGAACACT GAGCTCTATT CTCAAATCCG AACCAAAGCC GAGCGGCCCA GGGGCTGGGG 
     3721 GAGGAGTGGG CCTGGTTGCG CCCGGGGCCG GGGTCACCGC TGTGACCTCC CCTTTTACTA 
     3781 AGGTTGAGCG CACCTTCGTG CACATCGCTG AGAAGTCACA CCTCAATGTG ATGAGTTCCG 
     3841 GCGGGCAGGC CTCTCGGCCA GAGGAGCTTT CCACCGGGGG CGAGCTCGGA CTTGAGGTGC 
     3901 TTTCTGAAGG GGGGATCGCA GAGGAGGGTG CTCCTGCTCC ACTTGAGAAT GGAATGGCTC 
     3961 TGGCTGGGCT GGATGGAACA GAGATGGAAA GTTGCGCTCT CTCTGGACCA CCAGGAGAGA 
     4021 CACCTTCTGA AGTGGTGACG GATAGCCTCC CAAACGGGCC AGCCCTGGCC GACGGACCTG 
     4081 CTCCCGCCAG CCAACAGGAG CCGGTGACCA AAAAAGGTAC CACCATATCA CCTTCACGCC 
     4141 ACGCCATGCC CGGTAGCCGC CCCCGGTCCA GAATTCCAGT GCTGCTTTCA GAGGAAGATA 
     4201 CCGGTTCTGA GCCCAGCGGG AGCCTGAGTG CCAAGGAGAG ATGGAGTAAG AGAGCACGCC 
     4261 CACAGCAGGA CTTGGCCAGG CTGGTCATGG AGAAACGGCA GGGCAGGCTC CTCCTGAGAC 
     4321 TGGCTAGCGG CGCCTCTTCA AGCAGCAGTG AGGAACAGCG GAGGGCCAGC GAAACCCTGA 
     4381 GTGGCACCGG CAGCGAGGAG GACACCCCCG CCTCTGAGCC TACTACTGCT CTCCCACGCA 
     4441 AGGCCGTGCG CGCTGCCACG ACCCGGTCAC GCATCCCGCG GCCCATCAGC GTATCTATGC 
     4501 CTGTTGAGGG CCAACAGCTC CCAGGCAGGC CACACGGAGC TGCATCAGCA ACGGATCTCG 
     4561 CAATCACGAG TAGGCTGCAG TTGCAGAAGC CATCTGGGCT GGCGCCAGCT GCTGATCTCC 
     4621 GGCCTAAACA GTCCGCTTCC AGGGGCCCGG GCCCTGGCCG CGCCCAAGTG TCTAAGCCTG 
     4681 CCGCCCCGAG ATCACCCGGC CTGCCAGCAT CTACTGCAAG GCATCCCTCC GGATCACCGA 
     4741 GGAGCCAGAG CCTTAGCAGG AAAGAGTCTA GTAGCCCCTC CCACCAGGCC CGGCCTGGGG 
     4801 TGCCCCCATC TCGCGGCGTC CTCCAGGTGC GGTCCCAACC AGAGGCAAGT CCTGTGGCCC 
     4861 CTAAGAAGGG GCCTAAGGGA AAGCAGCTGC AGACTCAGCG GGCCGCCACA AAGGGCCGGG 
     4921 CCGTAGTATC TGAGGGCAGG CCTGGGGCAA GAACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     4981 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     5041 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     5101 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     5161 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     5221 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     5281 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     5341 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     5401 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     5461 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     5521 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     5581 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     5641 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     5701 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     5761 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     5821 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     5881 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     5941 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     6001 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     6061 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     6121 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     6181 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     6241 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     6301 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     6361 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     6421 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     6481 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     6541 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     6601 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     6661 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     6721 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     6781 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     6841 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     6901 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     6961 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     7021 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     7081 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     7141 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     7201 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     7261 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     7321 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     7381 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     7441 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     7501 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     7561 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     7621 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     7681 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     7741 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     7801 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     7861 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     7921 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     7981 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     8041 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     8101 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     8161 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     8221 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     8281 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     8341 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     8401 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     8461 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     8521 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     8581 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     8641 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     8701 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     8761 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//