LOCUS       MR227101                7923 bp    DNA     circular  SYN 
DEFINITION  Kl (Myc-DDK-tagged) - Mouse klotho (Kl)
ACCESSION   NM_013823
VERSION     NM_013823.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4080..4171 
                     /label="Myc-DDK"  
     misc_feature    4201..4786 
                     /label="PolyA signal"  
     misc_feature    4258..4277 
                     /label="Restriction Enzyme"  
     misc_feature    5062..5734 
                     /label="ColE1"  
     misc_feature    6164..6958 
                     /label="Kan/Neo"  
     misc_feature    6993..7295 
                     /label="SV40 ori"  
     misc_feature    7381..7858 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4062..4068 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4062 
                     /label="Kl"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCTAGCCCGC GCCCCTCCTC GCCGCCCGCC GCGGCTGGTG CTGCTCCGTT 
     1081 TGCTGTTGCT GCATCTGCTG CTGCTCGCCC TGCGCGCCCG CTGCCTGAGC GCTGAGCCGG 
     1141 GTCAGGGCGC GCAGACCTGG GCTCGCTTCG CGCGCGCTCC TGCCCCAGAG GCCGCTGGCC 
     1201 TCCTCCACGA CACCTTCCCC GACGGTTTCC TCTGGGCGGT AGGCAGCGCC GCCTATCAGA 
     1261 CCGAGGGCGG CTGGCGACAG CACGGCAAAG GCGCGTCCAT CTGGGACACT TTCACCCATC 
     1321 ACTCTGGGGC GGCCCCGTCC GACTCCCCGA TCGTCGTGGC GCCGTCGGGT GCCCCGTCGC 
     1381 CTCCCCTGTC CTCCACTGGA GATGTGGCCA GCGATAGTTA CAACAACGTC TACCGCGACA 
     1441 CAGAGGGGCT GCGCGAACTG GGGGTCACCC ACTACCGCTT CTCCATATCG TGGGCGCGGG 
     1501 TGCTCCCCAA TGGCACCGCG GGCACTCCCA ACCGCGAGGG GCTGCGCTAC TACCGGCGGC 
     1561 TGCTGGAGCG GCTGCGGGAG CTGGGCGTGC AGCCGGTGGT TACCCTGTAC CATTGGGACC 
     1621 TGCCACAGCG CCTGCAGGAC ACCTATGGCG GATGGGCCAA TCGCGCCCTG GCCGACCATT 
     1681 TCAGGGATTA TGCCGAGCTC TGCTTCCGCC ACTTCGGTGG TCAGGTCAAG TACTGGATCA 
     1741 CCATTGACAA CCCCTACGTG GTGGCCTGGC ACGGGTATGC CACCGGGCGC CTGGCCCCGG 
     1801 GCGTGAGGGG CAGCTCCAGG CTCGGGTACC TGGTTGCCCA CAACCTACTT TTGGCTCATG 
     1861 CCAAAGTCTG GCATCTCTAC AACACCTCTT TCCGCCCCAC ACAGGGAGGC CGGGTGTCTA 
     1921 TCGCCTTAAG CTCCCATTGG ATCAATCCTC GAAGAATGAC TGACTATAAT ATCAGAGAAT 
     1981 GCCAGAAGTC TCTTGACTTT GTGCTAGGCT GGTTTGCCAA ACCCATATTT ATTGATGGCG 
     2041 ACTACCCAGA GAGTATGAAG AACAACCTCT CGTCTCTTCT GCCTGATTTT ACTGAATCTG 
     2101 AGAAGAGGCT CATCAGAGGA ACTGCTGACT TTTTTGCTCT CTCCTTCGGA CCAACCTTGA 
     2161 GCTTTCAGCT ATTGGACCCT AACATGAAGT TCCGCCAATT GGAGTCTCCC AACCTGAGGC 
     2221 AGCTTCTGTC TTGGATAGAT CTGGAATATA ACCACCCTCC AATATTTATT GTGGAAAATG 
     2281 GCTGGTTTGT CTCGGGAACC ACCAAAAGGG ATGATGCCAA ATATATGTAT TATCTCAAGA 
     2341 AGTTCATAAT GGAAACCTTA AAAGCAATCA GACTGGATGG GGTCGACGTC ATTGGGTACA 
     2401 CCGCGTGGTC GCTCATGGAC GGTTTCGAGT GGCATAGGGG CTACAGCATC CGGCGAGGAC 
     2461 TCTTCTACGT TGACTTTCTG AGTCAGGACA AGGAGCTGTT GCCAAAGTCT TCGGCCTTGT 
     2521 TCTACCAAAA GCTGATAGAG GACAATGGCT TTCCTCCTTT ACCTGAAAAC CAGCCCCTTG 
     2581 AAGGGACATT TCCCTGTGAC TTTGCTTGGG GAGTTGTTGA CAACTACGTT CAAGTGGACA 
     2641 CTACTCTCTC TCAGTTTACT GACCCGAATG TCTATCTGTG GGATGTGCAT CACAGTAAGA 
     2701 GGCTTATTAA AGTAGACGGG GTTGTAGCCA AGAAGAGAAA ACCTTACTGT GTTGATTTCT 
     2761 CTGCCATCCG GCCTCAGATA ACCTTACTTC GAGAAATGCG GGTCACCCAC TTTCGCTTCT 
     2821 CCCTGGACTG GGCCCTGATC TTGCCTCTGG GTAACCAGAC CCAAGTGAAC CACACGGTTC 
     2881 TGCACTTCTA CCGCTGCATG ATCAGCGAGC TGGTGCACGC CAACATCACT CCAGTGGTGG 
     2941 CCCTGTGGCA GCCAGCAGCC CCGCACCAAG GCCTGCCACA TGCCCTTGCA AAACATGGGG 
     3001 CCTGGGAGAA CCCGCACACT GCTCTGGCGT TTGCAGACTA CGCAAACCTG TGTTTTAAAG 
     3061 AGTTGGGTCA CTGGGTCAAT CTCTGGATCA CCATGAACGA GCCAAACACA CGGAACATGA 
     3121 CCTATCGTGC CGGGCACCAC CTCCTGAGAG CCCATGCCTT GGCTTGGCAT CTGTACGATG 
     3181 ACAAGTTTAG GGCGGCTCAG AAAGGCAAAA TATCCATCGC CTTGCAGGCT GACTGGATAG 
     3241 AACCGGCCTG CCCTTTCTCT CAAAATGACA AAGAAGTGGC CGAGAGAGTT TTGGAATTTG 
     3301 ATATAGGCTG GCTGGCAGAG CCTATTTTTG GTTCCGGAGA TTATCCACGT GTGATGAGGG 
     3361 ACTGGCTGAA CCAAAAAAAC AATTTTCTTT TGCCCTATTT CACCGAAGAT GAAAAAAAGC 
     3421 TAGTCCGGGG TTCCTTTGAC TTCCTGGCGG TGAGTCATTA CACCACCATT CTGGTAGACT 
     3481 GGGAAAAGGA GGATCCGATG AAATACAACG ATTACTTGGA GGTACAGGAG ATGACTGACA 
     3541 TCACATGGCT CAACTCTCCC AGTCAGGTGG CAGTGGTGCC TTGGGGGCTG CGCAAAGTGC 
     3601 TCAACTGGCT AAGGTTCAAG TACGGAGACC TCCCGATGTA TGTGACAGCC AATGGAATCG 
     3661 ATGATGACCC CCACGCCGAG CAAGACTCAC TGAGGATCTA TTATATTAAG AATTATGTGA 
     3721 ATGAGGCTCT GAAAGCCTAC GTGTTGGACG ACATCAACCT TTGTGGCTAC TTTGCGTATT 
     3781 CACTTAGTGA TCGCTCAGCT CCCAAGTCTG GCTTTTATCG ATATGCTGCG AATCAGTTTG 
     3841 AGCCCAAACC ATCTATGAAA CATTACAGGA AAATTATTGA CAGCAATGGC TTCCTGGGTT 
     3901 CTGGAACACT GGGAAGGTTT TGTCCAGAAG AATACACTGT GTGCACCGAA TGTGGATTTT 
     3961 TTCAAACCCG GAAGTCTTTG CTGGTCTTCA TCTCGTTTCT TGTTTTTACT TTTATTATTT 
     4021 CTCTTGCTCT CATTTTTCAC TACTCCAAGA AAGGCCAGAG AAGTTATAAG ACGCGTACGC 
     4081 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     4141 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     4201 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     4261 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     4321 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     4381 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     4441 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     4501 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     4561 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     4621 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     4681 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     4741 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     4801 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     4861 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     4921 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     4981 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     5041 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     5101 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     5161 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     5221 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     5281 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     5341 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     5401 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     5461 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     5521 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     5581 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     5641 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     5701 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     5761 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     5821 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     5881 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     5941 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     6001 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     6061 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     6121 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     6181 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     6241 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     6301 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     6361 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     6421 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     6481 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     6541 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     6601 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     6661 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     6721 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     6781 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     6841 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     6901 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     6961 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     7021 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     7081 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     7141 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     7201 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     7261 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     7321 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     7381 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     7441 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     7501 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     7561 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     7621 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     7681 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     7741 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     7801 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     7861 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     7921 TTT
//