LOCUS       MR227091                8769 bp    DNA     circular  SYN 
DEFINITION  Ehmt1 (Myc-DDK-tagged) - Mouse euchromatic histone methyltransferase 1 (Ehmt1), transcript variant 1
ACCESSION   NM_001012518
VERSION     NM_001012518.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4926..5017 
                     /label="Myc-DDK"  
     misc_feature    5047..5632 
                     /label="PolyA signal"  
     misc_feature    5104..5123 
                     /label="Restriction Enzyme"  
     misc_feature    5908..6580 
                     /label="ColE1"  
     misc_feature    7010..7804 
                     /label="Kan/Neo"  
     misc_feature    7839..8141 
                     /label="SV40 ori"  
     misc_feature    8227..8704 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4908..4914 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4908 
                     /label="Ehmt1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCGCCGCT GATGCTGAGC AGGCAGTTCT GGCCAAGCAA GAGACCAAGC 
     1081 AGGATTGCTG CATGAAAACT GAGCTGCTAA GGGAAGATAC ACCTATGGCT GCTGATGAAG 
     1141 GTTCCACAGA GAAACAAGAA GGAGAGACTC CCATGGCTGC AGATGGAGAA ACAAATGGGT 
     1201 CTTGTGAAAA GAGTGGGGAT CCCAGCCATC TAAATGCACC CAAACACACT CAGGAGAACA 
     1261 CAAGAGCTAG CCCACAGGAA GGCACCAACA GAGTGTCTCG GGTGGCAGAA AATGGGGTTT 
     1321 CAGAAAGAGA CACAGAAGTG GGGAAGCAAA ACCATGTCAC AGCTGACGAC TTCATGCAGA 
     1381 CATCTGTCAT TGGCAGCAAT GGATATTTCT TAAATAAACC AGCCCTGCAG GGGCAGCCGT 
     1441 TGAGGACTCC CAACATTCTA ACCTCCTCGC TTCCTGGTCA TGCTGCAAAA ACTCTTCCTG 
     1501 GAGGAGCCAG TAAATGCAGG ACTCTGAGTG CACTTCCTCA GACACCAACC ACAGCACCCA 
     1561 CTGTGCCTGG GGAAGGGAGT GCAGACACAG AGGACAGAAA GCCTACAGCC TCGGGCACTG 
     1621 ATGTCAGGGT TCACAGGGCA CGCAAGACCA TGCCGAAGTC CATCTTGGGC CTGCATGCAG 
     1681 CCAGCAAAGA CCATAGAGAA GTTCAAGACC ATAAGGAACC AAAAGAGGAT ATCAACAGAA 
     1741 ACATTTCTGA ATGTGGACGA CAGCAGCTTT TACCAACCTT CCCAGCCCTC CACCAGTCGC 
     1801 TACCTCAGAA TCAGTGCTAC ATGGCCACCA CAAAGTCCCA GACAGCTTGC TTGCCTTTTG 
     1861 TTTTAGCAGC TGCAGTATCT CGGAAGAAAA AACGAAGAAT GGGAACCTAT AGTTTAGTTC 
     1921 CCAAGAAAAA GACCAAAGTA TTAAAACAGA GGACGGTGAT TGAGATGTTT AAAAGCATAA 
     1981 CCCATTCCAC TGTGGGCGCC AAGGGCGAGA AAGCCTTAGA TGATAGTGCC CTGCATGTAA 
     2041 ATGGCGAGAG CTTGGAGATG GACTCAGAAG ATGAAGACTC CGATGAGCTG GAGGATGACG 
     2101 AGGACCATGG AGCTGAGCAA GCGGCTGCAT TTCCCACCGA GGATAGTAGG ACTTCTAAAG 
     2161 AGAGCATGTC TGAGACTGAC CGGGCTGCAA AGATGGATGG AGATTCAGAG GAGGAACAGG 
     2221 AGTCTCCCGA CACAGGGGAG GATGAAGATG GTGGAGACGA GTCTGACCTG AGTTCTGAAT 
     2281 CCAGTATCAA GAAGAAATTT CTCAAGAGGA GAGGGAAGAC TGACAGCCCC TGGATCAAAC 
     2341 CTGCTCGGAA AAGGAGGCGG AGAAGTAGAA AGAAGCCGAG CAGCATGCTT GGTTCCGAGG 
     2401 CTTGTAAGTC ATCTCCAGGA AGCATGGAGC AGGCAGCTCT GGGAGACAGC GCTGGCTATA 
     2461 TGGAAGTTTC CCTGGATTCC CTGGATCTCC GTGTCAGAGG AATTCTGTCT TCCCAGACAG 
     2521 AAAATGAAGG GCTGGCCAGT GGTCCGGATG TGCTGGGGAC GGATGGCCTC CAGGAAGTGC 
     2581 CTCTCTGCAG CTGCCGAATG GAAACCCCCA AGAGCCGCGA GATCAGCACC CTGGCCAACA 
     2641 ACCAGTGCAT GGCCACTGAG AGCGTGGATC ACGAATTGGG CCGGTGTACA AACAGCGTGG 
     2701 TCAAGTATGA GCTGATGCGC CCATCCAACA AAGCACCGCT CTTGGTGTTA TGTGAAGACC 
     2761 ATCGGGGTCG CATGGTGAAG CACCAGTGCT GTCCTGGCTG CGGCTACTTC TGCACGGCGG 
     2821 GTAACTTCAT GGAATGCCAG CCTGAGAGCA GCATCTCTCA TCGTTTCCAT AAGGACTGTG 
     2881 CCTCTCGAGT CAACAATGCC AGCTACTGTC CCCATTGTGG GGAAGAAGCT TCCAAGGCCA 
     2941 AAGAGGTGAC CATAGCAAAA GCAGACACAA CTTCCACAGT GACCCTAGCC CCTGGACAGG 
     3001 AGAAGAGCCT GGCTGCTGAA GGCAGGGCTG ACACGACCAC GGGCAGCATT GCTGGAGCCC 
     3061 CAGAGGATGA AAGATCGCAG AGCACAGCCC CCCAGGCACC AGAGTGCTTC GACCCTGCCG 
     3121 GACCGGCTGG GCTCGTGAGG CCGACATCTG GCCTTTCCCA GGGCCCAGGA AAGGAAACCT 
     3181 TGGAAAGTGC TCTAATCGCT CTAGACTCTG AAAAACCCAA GAAACTTCGC TTCCACCCAA 
     3241 AGCAGCTGTA CTTCTCTGCC AGGCAGGGTG AGCTGCAGAA GGTGCTTCTC ATGCTGGTTG 
     3301 ATGGAATTGA TCCCAACTTC AAAATGGAGC ACCAAAGTAA GCGTTCCCCA TTACATGCTG 
     3361 CTGCGGAGGC TGGCCACGTG GACATCTGCC ACATGCTGGT TCAGGCGGGT GCCAATATTG 
     3421 ACACTTGCTC AGAGGACCAG CGGACCCCAC TGATGGAGGC TGCAGAGAAC AACCACTTGG 
     3481 ATGCAGTGAA GTACCTCATC AAGGCTGGAG CACAGGTGGA TCCGAAGGAC GCAGAGGGCT 
     3541 CCACATGTTT GCATTTGGCT GCCAAGAAAG GCCACTATGA TGTGGTTCAG TATCTGCTTT 
     3601 CAAATGGACA GATGGATGTC AACTGCCAGG ATGACGGTGG ATGGACACCT ATGATCTGGG 
     3661 CCACTGAGTA CAAGCACGTG GAGCTGGTGA AGCTGCTGCT GTCTAAGGGC TCTGACATCA 
     3721 ACATCCGGGA CAACGAGGAG AACATTTGTC TGCACTGGGC AGCATTTTCA GGCTGTGTGG 
     3781 ACATAGCTGA AATACTTCTG GCTGCCAAGT GTGACCTGCA TGCTGTGAAT ATCCATGGAG 
     3841 ACTCACCCCT GCACATCGCA GCCAGGGAGA ATCGCTACGA CTGTGTTGTC CTCTTTCTTT 
     3901 CTCGGGATTC AGATGTTACT CTGAAAAACA AGGAAGGAGA GACTCCCTTG CAGTGTGCAA 
     3961 GTCTCAGTTC GCAGGTGTGG AGTGCATTGC AGATGAGCAA AGCACTTCGG GACTCAGCCC 
     4021 CTGACAAGCC CGTTGCTGTT GAGAAGACGG TGAGCAGGGA TATCGCTCGA GGGTATGAGC 
     4081 GCATTCCCAT TCCCTGTGTC AATGCTGTGG ACAGTGAGCT GTGTCCTACC AACTATAAGT 
     4141 ATGTCTCCCA GAACTGTGTG ACATCCCCCA TGAACATTGA CAGGAACATC ACTCATTTGC 
     4201 AGTACTGCGT GTGTGTAGAT GACTGCTCCT CTAGCACCTG CATGTGTGGC CAGCTGAGCA 
     4261 TGCGCTGCTG GTATGATAAG GATGGCCGAC TTCTGCCTGA GTTTAACATG GCAGAACCAC 
     4321 CCTTGATCTT CGAGTGCAAT CATGCCTGCT CATGCTGGAG GAACTGCCGC AATCGTGTGG 
     4381 TGCAAAATGG TCTCAGGGCA AGGCTGCAGC TTTATCGGAC ACAGGACATG GGCTGGGGTG 
     4441 TGCGGTCCCT CCAGGATATC CCACTGGGCA CCTTTGTCTG CGAATACGTA GGGGAGCTGA 
     4501 TTTCGGACTC TGAAGCTGAT GTTCGGGAAG AGGACTCTTA CCTCTTTGAT CTTGACAATA 
     4561 AGGATGGAGA GGTATACTGC ATTGACGCTC GGTTCTATGG GAATGTCAGC CGGTTCATAA 
     4621 ACCACCACTG CGAACCCAAC CTTGTGCCTG TGCGAGTGTT CATGTCACAC CAGGACCTGC 
     4681 GGTTTCCCAG GATTGCCTTC TTCAGTACCC GCCTGATTCA GGCTGGGGAG CAGCTCGGGT 
     4741 TCGACTACGG GGAGCGCTTT TGGGACGTCA AGGGCAAGCT CTTCAGTTGC CGGTGTGGGT 
     4801 CTTCCAAGTG TCGGCACTCA AGCGCAGCCC TGGCCCAGAG GCAAGCCAGT GCAGCCCAGG 
     4861 AGCCTCAGGA GAATGGCCTT CCAGATACCA GCTCTGCAGC CGCTGCTGAC CCCCTAACGC 
     4921 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     4981 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     5041 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     5101 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     5161 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     5221 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     5281 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     5341 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     5401 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     5461 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     5521 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     5581 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     5641 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     5701 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     5761 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     5821 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     5881 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     5941 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     6001 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     6061 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     6121 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     6181 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     6241 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     6301 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     6361 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     6421 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     6481 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     6541 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     6601 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     6661 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     6721 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     6781 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     6841 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     6901 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     6961 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     7021 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     7081 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     7141 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     7201 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     7261 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     7321 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     7381 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     7441 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     7501 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     7561 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     7621 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     7681 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     7741 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     7801 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     7861 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     7921 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     7981 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     8041 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     8101 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     8161 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     8221 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     8281 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     8341 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     8401 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     8461 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     8521 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     8581 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     8641 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     8701 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     8761 GCGAATTTT
//