LOCUS       MR227085                7869 bp    DNA     circular  SYN 
DEFINITION  Ephb4 (Myc-DDK-tagged) - Mouse Eph receptor B4 (Ephb4), transcript variant 1
ACCESSION   NM_001159571
VERSION     NM_001159571.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4026..4117 
                     /label="Myc-DDK"  
     misc_feature    4147..4732 
                     /label="PolyA signal"  
     misc_feature    4204..4223 
                     /label="Restriction Enzyme"  
     misc_feature    5008..5680 
                     /label="ColE1"  
     misc_feature    6110..6904 
                     /label="Kan/Neo"  
     misc_feature    6939..7241 
                     /label="SV40 ori"  
     misc_feature    7327..7804 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4008..4014 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4008 
                     /label="Ephb4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGCTCCGA GCGCTGCTGT GCTGGGCTTC CCTCGCCACT GCTTTAGAAG 
     1081 AGACCCTGTT GAACACAAAA CTGGAAACGG CGGATCTGAA ATGGGTGACT TACCCTCAGG 
     1141 CAGAGGGCCA GTGGGAGGAG CTAAGCGGCC TGGATGAGGA ACAGCACAGC GTCCGCACCT 
     1201 ATGAGGTGTG CGACATGAAG CGTCCAGGGG GCCAGGCTCA CTGGCTGCGC ACTGGCTGGG 
     1261 TCCCAAGGCG AGGTGCTGTC CACGTGTATG CCACGATACG CTTCACCATG ATGGAATGCC 
     1321 TGTCCCTGCC GAGGGCCAGT CGCTCCTGCA AGGAGACATT CACTGTCTTC TATTACGAGA 
     1381 GCGAAGCTGA TACGGCCACG GCCCATACGC CCGCCTGGAT GGAGAACCCC TACATCAAGG 
     1441 TGGACACAGT GGCCGCAGAA CATCTGACTC GGAAGCGCCC TGGAGCTGAA GCCACAGGGA 
     1501 AAGTTAATAT CAAGACGCTG CGCCTGGGTC CTCTCAGCAA AGCTGGCTTC TACCTGGCTT 
     1561 TCCAGGACCA AGGAGCCTGC ATGGCTCTGC TCTCCCTGCA TCTCTTTTAC AAGAAGTGCT 
     1621 CCTGGCTGAT CACGAACTTG ACCTACTTCC CCGAGACGGT GCCTCGGGAG CTCGTGGTGC 
     1681 CGGTGGCAGG TAGCTGCGTG GCCAACGCGG TCCCTACCGC CAACCCCAGC CCCAGCCTCT 
     1741 ACTGCCGGGA AGATGGTCAA TGGGCTGAGC AGCAGGTCAC GGGCTGCAGC TGCGCGCCAG 
     1801 GGTACGAGGC TGCGGAAAGC AACAAAGTAT GCAGAGCCTG TGGCCAGGGA ACCTTCAAGC 
     1861 CCCAAATAGG AGACGAGTCC TGCCTGCCAT GCCCAGCCAA CAGCCACTCG AATAACATTG 
     1921 GGTCTCCTGT CTGCCTGTGT CGAATTGGGT ATTACCGGGC CCGCTCAGAC CCCCGGAGTT 
     1981 CACCTTGCAC TACCCCACCC TCTGCTCCAA GAAGCGTGGT TCACCATTTG AATGGTTCCA 
     2041 CCCTGCGCCT GGAATGGAGT GCTCCCCTTG AGTCCGGAGG CCGAGAGGAC CTCACTTATG 
     2101 CTGTACGCTG CCGAGAGTGC CGTCCTGGGG GTTCCTGCTT GCCCTGTGGG GGCGACATGA 
     2161 CCTTCGACCC CGGTCCTCGA GACCTGGTTG AGCCCTGGGT GGCAATCCGA GGGCTGCGTC 
     2221 CTGATGTCAC CTATACCTTT GAGGTTGCTG CTTTGAATGG TGTGTCTACC TTAGCCACTG 
     2281 GACCACCTCC TTTTGAGCCT GTCAATGTCA CCACTGACCG TGAGGGGCGC ATTGGGTTTC 
     2341 TTTCTCCTGC AGTGCCTCCT GCAGTGTCTG ACATCCGAGT GACTCGGTCG TCACCCAGCA 
     2401 GCTTGATCCT GTCATGGGCT ATCCCCAGAG CACCCAGTGG GGCCGTGCTG GACTACGAGG 
     2461 TCAAGTATCA TGAGAAGGGC GCAGAGGGCC CCAGCAGTGT TCGTTTCCTG AAGACATCAG 
     2521 AAAACCGAGC TGAGCTCCGG GGGCTGAAGC GGGGAGCCAG CTATCTGGTC CAGGTACGCG 
     2581 CACGGTCCGA GGCTGGCTAC GGTCCCTTCG GCCAGGAGCA TCACAGTCAG ACTCAACTGG 
     2641 ATGAGAGCGA GAGCTGGCGG GAGCAGCTGG CCCTGATTGC AGGCACTGCG GTTGTGGGTG 
     2701 TGGTCCTGGT CCTGGTGGTC GTCATCATTG CAGTTCTCTG CCTCAGGAAG CAGAGCAATG 
     2761 GGAGGGAAGT TGAGTACTCG GATAAGCATG GGCAGTATCT CATCGGGCAC GGTACCAAGG 
     2821 TCTACATTGA TCCTTTTACT TACGAAGACC CTAATGAGGC AGTGAGGGAA TTTGCCAAAG 
     2881 AGATCGATGT CTCCTATGTC AAGATTGAAG AGGTAATTGG TGCAGGTGAG TTCGGCGAGG 
     2941 TGTGCCGGGG TCGGCTGAAG GCACCAGGGA AAAAGGAGAG CTGTGTGGCC ATCAAGACTC 
     3001 TGAAGGGTGG CTACACCGAG CGCCAGAGGC GTGAGTTCCT GAGCGAGGCC TCCATCATGG 
     3061 GCCAGTTCGA GCATCCCAAC ATCATCCGCC TCGAGGGCGT GGTCACCAAC AGTGTGCCGG 
     3121 TTATGATCCT CACGGAATTC ATGGAGAACG GAGCCCTGGA CTCCTTCCTG CGGCTGAACG 
     3181 ACGGGCAGTT CACAGTCATC CAGCTGGTGG GCATGCTGAG GGGCATCGCC TCGGGCATGC 
     3241 GGTACCTGGC TGAAATGAGC TATGTCCACC GAGACCTGGC TGCTCGGAAC ATCTTGGTCA 
     3301 ACAGTAACCT GGTCTGCAAG GTGTCCGACT TTGGCCTCTC CAGATTCTTG GAGGAGAACT 
     3361 CCTCTGATCC CACCTACACA AGTTCCCTGG GAGGCAAGAT TCCCATCCGA TGGACCGCCC 
     3421 CTGAAGCCAT TGCCTTCAGG AAGTTCACCT CTGCCAGTGA TGCCTGGAGC TATGGGATCG 
     3481 TCATGTGGGA GGTCATGTCT TTTGGGGAAC GGCCATACTG GGACATGAGC AACCAGGATG 
     3541 TGATCAATGC CATTGAACAG GACTACCGGC TGCCTCCTCC TCCAGACTGC CCCACCTCCC 
     3601 TCCACCAGCT CATGCTGGAT TGTTGGCAGA AGGACCGGAA TGCCCGGCCC CGCTTTCCCC 
     3661 AGGTGGTCAG CGCTCTGGAC AAGATGATCC GGAATCCCGC TAGCCTCAAA ATCGTGGCCA 
     3721 GGGAGAATGG CGGGGCCTCA CATCCACTCT TGGACCAACG GCAGCCTCAC TATTCTGCTT 
     3781 TCGGTTCTGT GGGCGAGTGG CTTCGAGCCA TCAAGATGGG AAGATACGAG GAAAGTTTTG 
     3841 CAGCGGCTGG ATTCGGCTCC TTTGAGGTGG TCAGTCAGAT CTCTGCCGAG GACCTTCTCC 
     3901 GAATTGGAGT CACTCTGGCA GGACACCAGA AGAAAATCTT GGCCAGTGTG CAGCATATGA 
     3961 AGTCCCAAGC TAAGCCAGGA GCCCCTGGTG GGACAGGGGG ACCAGCCCAG CAGTTCACGC 
     4021 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     4081 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     4141 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     4201 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     4261 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     4321 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     4381 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     4441 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     4501 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     4561 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     4621 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     4681 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     4741 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     4801 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     4861 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     4921 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     4981 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     5041 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     5101 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     5161 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     5221 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     5281 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     5341 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     5401 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     5461 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     5521 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     5581 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     5641 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     5701 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     5761 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     5821 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     5881 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     5941 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     6001 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     6061 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     6121 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     6181 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     6241 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     6301 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     6361 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     6421 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     6481 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     6541 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     6601 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     6661 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     6721 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     6781 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     6841 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     6901 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     6961 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     7021 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     7081 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     7141 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     7201 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7261 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     7321 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     7381 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     7441 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     7501 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     7561 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     7621 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     7681 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     7741 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     7801 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     7861 GCGAATTTT
//