LOCUS       MR227067                8997 bp    DNA     circular  SYN 
DEFINITION  Insr (Myc-DDK-tagged) - Mouse insulin receptor (Insr)
ACCESSION   NM_010568
VERSION     NM_010568.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5154..5245 
                     /label="Myc-DDK"  
     misc_feature    5275..5860 
                     /label="PolyA signal"  
     misc_feature    5332..5351 
                     /label="Restriction Enzyme"  
     misc_feature    6136..6808 
                     /label="ColE1"  
     misc_feature    7238..8032 
                     /label="Kan/Neo"  
     misc_feature    8067..8369 
                     /label="SV40 ori"  
     misc_feature    8455..8932 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5136..5142 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5136 
                     /label="Insr"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGCTTCGGG AGAGGATGTG AGACGACGGC TGTGCCATTG CTGGTGGCCG 
     1081 TGGCCGCGTT GCTGGTGGGC ACAGCCGGCC ACCTGTACCC TGGAGAGGTG TGCCCTGGTA 
     1141 TGGACATCCG GAACAACCTG ACCAGGCTAC ATGAGCTGGA GAACTGCTCA GTCATTGAGG 
     1201 GCCATCTGCA GATCCTCCTG ATGTTCAAGA CCAGACCCGA AGATTTCCGA GACCTCAGTT 
     1261 TCCCCAAACT CATCATGATC ACAGATTACC TGCTTCTCTT CCGTGTCTAT GGTCTGGAAA 
     1321 GTCTGAAAGA CCTCTTCCCA AATCTCACAG TCATCCGAGG CTCCCGTCTC TTCTTCAACT 
     1381 ATGCCCTGGT TATCTTCGAG ATGGTCCACC TGAAGGAGCT GGGGCTTTAT AACCTCATGA 
     1441 ACATCACCCG GGGCTCTGTC CGCATCGAGA AGAATAATGA GCTCTGCTAC CTGGCCACTA 
     1501 TCGACTGGTC CCGTATCCTG GATTCTGTGG AGGACAACTA CATTGTACTG AACAAAGATG 
     1561 ACAACGAGGA ATGTGGGGAT GTCTGTCCAG GCACCGCCAA GGGCAAGACC AACTGTCCTG 
     1621 CCACTGTCAT CAATGGGCAG TTTGTGGAAC GGTGCTGGAC ACACAGTCAT TGTCAGAAAG 
     1681 TTTGCCCAAC CATCTGTAAG TCACATGGCT GCACAGCTGA AGGCCTGTGC TGCCACAAAG 
     1741 AGTGCCTGGG CAACTGTTCG GAACCTGATG ACCCCACCAA GTGTGTGGCC TGTCGCAACT 
     1801 TCTATCTGGA TGGTCAGTGT GTGGAGACCT GCCCGCCACC CTACTATCAC TTCCAGGACT 
     1861 GGCGCTGTGT GAACTTCAGC TTCTGCCAAG ACCTTCACTT CAAATGCAGG AACTCTCGGA 
     1921 AGCCTGGCTG CCACCAATAC GTCATTCACA ACAATAAGTG CATCCCCGAG TGCCCGTCTG 
     1981 GCTATACCAT GAATTCCAGC AACTTGATGT GCACCCCATG TCTGGGACCC TGCCCTAAGG 
     2041 TCTGCCAAAT CCTCGAAGGT GAGAAGACCA TTGATTCTGT GACATCTGCC CAGGAGCTCC 
     2101 GAGGCTGCAC TGTGATCAAC GGTAGCCTGA TCATCAACAT CCGAGGGGGC AACAACCTGG 
     2161 CAGCTGAGCT GGAGGCTAAC CTTGGCCTCA TTGAAGAAAT TTCGGGATTT CTAAAGATCC 
     2221 GCCGCTCCTA TGCTCTGGTA TCACTTTCTT TCTTCAGGAA GCTACATCTG ATTCGAGGAG 
     2281 AGACCTTGGA AATTGGGAAC TATTCTTTTT ATGCCTTGGA CAACCAGAAC CTGAGGCAAC 
     2341 TCTGGGACTG GAGCAAACAC AACCTCACCA TCACTCAGGG CAAGCTCTTC TTCCATTACA 
     2401 ACCCGAAACT CTGCTTGTCT GAAATTCACA AGATGGAAGA AGTCTCCGGA ACTAAGGGCC 
     2461 GTCAGGAGAG GAACGACATT GCCCTGAAGA CCAATGGGGA CCAGGCATCG TGTGAAAATG 
     2521 AATTGCTTAA ATTTTCTTTC ATTCGGACAT CTTTTGACAA GATCCTGTTG AGGTGGGAAC 
     2581 CCTACTGGCC CCCCGACTTC CGAGATCTCC TGGGATTCAT GTTGTTCTAC AAAGAGGCCC 
     2641 CTTATCAGAA TGTGACAGAG TTTGATGGGC AGGATGCTTG TGGCTCCAAC AGCTGGACTG 
     2701 TGGTGGATAT TGACCCGCCC CAGAGGTCCA ACGACCCCAA GTCTCAGACC CCAAGCCACC 
     2761 CTGGGTGGCT GATGCGGGGC CTCAAACCCT GGACCCAATA CGCCATCTTT GTGAAGACCT 
     2821 TGGTTACCTT CTCTGATGAA CGGCGGACCT ATGGAGCCAA AAGTGATATC ATCTATGTGC 
     2881 AAACAGATGC CACTAATCCT TCTGTCCCCC TGGATCCCAT ATCAGTTTCT AATTCCTCAT 
     2941 CTCAGATTAT CTTAAAGTGG AAGCCCCCCT CTGACCCCAA TGGCAACATC ACACACTACC 
     3001 TGGTGTACTG GGAGAGGCAA GCAGAGGACA GCGAGCTGTT TGAGCTGGAT TATTGTCTCA 
     3061 AAGGGCTGAA GCTCCCTTCA CGGACCTGGT CCCCACCCTT TGAGTCTGAT GATTCTCAGA 
     3121 AGCACAATCA GAGTGAGTAT GACGACTCGG CCAGTGAGTG CTGCTCATGC CCTAAGACTG 
     3181 ACTCTCAGAT CCTGAAGGAG CTGGAGGAGT CTTCATTCAG GAAGACCTTC GAGGATTACC 
     3241 TGCACAACGT GGTTTTTGTC CCCAGGCCAT CCCGAAAGCG AAGATCCCTT GAAGAGGTGG 
     3301 GGAATGTGAC AGCCACCACA CTCACACTTC CAGATTTCCC CAACGTCTCC TCTACCATTG 
     3361 TGCCCACAAG TCAGGAGGAG CACAGGCCAT TTGAGAAAGT GGTGAACAAG GAGTCACTTG 
     3421 TCATCTCTGG CCTGAGACAC TTCACTGGGT ACCGCATTGA GCTGCAGGCA TGCAATCAAG 
     3481 ATTCCCCAGA TGAGAGGTGC AGTGTGGCTG CCTACGTCAG TGCCCGGACC ATGCCTGAAG 
     3541 CTAAGGCAGA TGACATCGTT GGCCCTGTGA CTCATGAAAT CTTTGAGAAC AATGTTGTAC 
     3601 ACTTAATGTG GCAAGAGCCA AAGGAACCTA ATGGTCTGAT TGTGCTATAT GAAGTGAGCT 
     3661 ATCGCCGATA TGGTGATGAG GAGCTGCACC TCTGTGTCTC CCGGAAGCAT TTTGCCCTGG 
     3721 AGCGGGGCTG CAGGCTGCGA GGGCTCTCCC CAGGAAACTA CAGTGTTCGA GTCCGGGCTA 
     3781 CCTCTCTGGC AGGAAATGGC TCCTGGACAG AACCCACCTA TTTTTATGTG ACTGATTATT 
     3841 TAGATGTCCC ATCAAATATT GCCAAAATTA TCATTGGACC CCTCATCTTT GTCTTCCTCT 
     3901 TCAGTGTTGT GATTGGAAGT ATTTATCTAT TTCTGAGAAA GAGGCAGCCG GATGGGCCAA 
     3961 TGGGACCACT GTATGCATCT TCAAACCCTG AGTACCTCAG TGCCAGTGAT GTGTTTCCAT 
     4021 CTTCTGTGTA CGTGCCGGAC GAGTGGGAGG TGCCTCGAGA GAAGATCACC CTTCTTCGAG 
     4081 AGCTGGGGCA GGGATCCTTT GGTATGGTGT ATGAAGGCAA TGCCAAGGAT ATCATCAAGG 
     4141 GTGAGGCAGA GACCCGTGTT GCGGTTAAGA CTGTCAATGA GTCAGCCAGT CTTCGAGAAC 
     4201 GGATCGAGTT CCTCAATGAG GCATCAGTCA TGAAGGGATT CACCTGCCAT CATGTGGTCC 
     4261 GCCTTCTTGG GGTGGTATCC AAAGGACAGC CAACGCTGGT AGTGATGGAA TTGATGGCTC 
     4321 ATGGAGACCT GAAAAGTCAC CTCCGTTCTC TGAGGCCAGA TGCTGAGAAT AACCCAGGCC 
     4381 GCCCTCCCCC TACCTTGCAA GAAATGATTC AGATGACAGC AGAAATTGCT GATGGCATGG 
     4441 CATACTTGAA CGCCAAGAAG TTTGTGCACC GGGACCTGGC AGCTCGAAAC TGCATGGTTG 
     4501 CCCATGATTT TACTGTCAAA ATTGGAGACT TTGGAATGAC AAGGGACATC TACGAGACAG 
     4561 ATTACTATCG GAAAGGGGGC AAGGGACTGC TTCCTGTGAG GTGGATGTCA CCTGAGTCCC 
     4621 TGAAGGATGG AGTCTTTACT GCTTCTTCTG ATATGTGGTC CTTTGGGGTG GTCCTTTGGG 
     4681 AAATCACTAG CCTGGCTGAG CAACCTTATC AAGGCCTGTC TAATGAACAG GTGTTGAAGT 
     4741 TTGTCATGGA TGGAGGCTAT CTGGATCCCC CTGATAACTG TCCAGAGAGA CTCACTGACC 
     4801 TGATGCGCAT GTGCTGGCAG TTCAACCCCA AGATGAGGCC AACCTTCCTG GAAATCGTCA 
     4861 ACCTGCTCAA GGATGACCTC CACCCCAGCT TTCCAGAAGT TTCCTTCTTC TACAGCGAGG 
     4921 AGAACAAGGC TCCTGAGAGT GAGGAGCTGG AGATGGAGTT TGAAGACATG GAGAATGTCC 
     4981 CGTTGGATCG TTCCTCTCAC TGTCAGAGAG AAGAGGCTGG GGGCCGGGAG GGAGGGTCCT 
     5041 CACTGAGCAT CAAACGGACC TATGATGAAC ACATCCCCTA TACCCACATG AATGGGGGCA 
     5101 AGAAGAACGG ACGTGTCCTT ACCCTGCCAA GGTCAAACCC TTCCACGCGT ACGCGGCCGC 
     5161 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     5221 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     5281 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     5341 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     5401 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     5461 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     5521 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     5581 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     5641 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     5701 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     5761 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     5821 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     5881 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     5941 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     6001 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     6061 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     6121 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     6181 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     6241 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     6301 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     6361 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     6421 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     6481 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     6541 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     6601 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     6661 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     6721 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     6781 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     6841 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     6901 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     6961 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     7021 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     7081 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     7141 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     7201 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     7261 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     7321 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     7381 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     7441 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     7501 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     7561 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     7621 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     7681 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     7741 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     7801 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     7861 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     7921 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     7981 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     8041 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     8101 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     8161 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     8221 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     8281 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     8341 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     8401 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     8461 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     8521 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     8581 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     8641 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     8701 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     8761 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     8821 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     8881 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     8941 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//