LOCUS       MR227063                6981 bp    DNA     circular  SYN 
DEFINITION  Scnn1a (Myc-DDK-tagged) - Mouse sodium channel, nonvoltage-gated 1 alpha (Scnn1a)
ACCESSION   NM_011324
VERSION     NM_011324.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3138..3229 
                     /label="Myc-DDK"  
     misc_feature    3259..3844 
                     /label="PolyA signal"  
     misc_feature    3316..3335 
                     /label="Restriction Enzyme"  
     misc_feature    4120..4792 
                     /label="ColE1"  
     misc_feature    5222..6016 
                     /label="Kan/Neo"  
     misc_feature    6051..6353 
                     /label="SV40 ori"  
     misc_feature    6439..6916 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3120..3126 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3120 
                     /label="Scnn1a"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATGCTGGAC CACACCAGAG CCCCTGAGCT CAACCTTGAC CTAGACCTTG 
     1081 ACGTCTCCAA CTCACCGAAG GGATCCATGA AGGGCAACAA TTTCAAGGAG CAAGACCTTT 
     1141 GTCCTCCTCT GCCCATGCAA GGACTGGGCA AGGGGGACAA GCGTGAAGAA CAGGCGCTGG 
     1201 GCCCGGAACC CTCAGAGCCC CGGCAGCCCA CCGAGGAGGA GGAGGCACTG ATCGAGTTCC 
     1261 ACCGCTCCTA CCGGGAGCTC TTCCAGTTCT TCTGCAACAA TACCACCATC CACGGTGCCA 
     1321 TCCGCCTGGT GTGCTCCAAG CACAACCGCA TGAAGACGGC CTTCTGGGCG GTGCTCTGGC 
     1381 TCTGCACCTT CGGCATGATG TACTGGCAGT TTGCTTTGCT GTTCGAGGAA TACTTCAGCT 
     1441 ACCCCGTGAG TCTCAACATC AACCTCAATT CGGACAAGCT GGTCTTCCCT GCCGTCACTG 
     1501 TGTGCACCCT TAATCCTTAC AGATACACTG AAATTAAAGA GGATCTGGAA GAGCTGGACC 
     1561 GCATCACGGA ACAGACGCTT TTTGACCTGT ACAAATACAA CTCTTCCTAC ACTCGCCAGG 
     1621 CTGGGGGCCG CCGCCGCAGC ACCCGCGACC TCCGGGGTGC TCTCCCACAC CCCCTGCAGC 
     1681 GCCTGCGCAC ACCACCTCCG CCCAATCCCG CCCGCTCGGC GCGCAGCGCG TCCTCCAGTG 
     1741 TACGCGACAA CAATCCCCAA GTGGACAGGA AGGACTGGAA AATCGGCTTC CAACTGTGCA 
     1801 ACCAGAACAA ATCAGACTGC TTCTACCAGA CATACTCATC CGGGGTGGAT GCCGTGAGAG 
     1861 AATGGTACCG CTTCCATTAC ATCAACATTC TGTCCAGACT GCCCGACACC TCGCCTGCTC 
     1921 TAGAGGAAGA AGCCCTGGGC AGCTTCATCT TTACCTGTCG TTTCAACCAG GCCCCCTGCA 
     1981 ATCAGGCGAA TTATTCTCAG TTCCACCACC CCATGTATGG GAACTGCTAC ACTTTCAACA 
     2041 ACAAGAACAA CTCCAATCTC TGGATGTCTT CCATGCCTGG AGTCAACAAT GGTTTGTCCC 
     2101 TGACACTGCG CACAGAGCAG AATGACTTCA TCCCCCTGCT GTCCACAGTG ACGGGGGCCA 
     2161 GGGTGATGGT GCACGGTCAG GATGAGCCTG CTTTTATGGA TGATGGTGGC TTCAACGTGA 
     2221 GGCCTGGTGT GGAGACCTCC ATCAGTATGA GAAAGGAAGC CCTGGACAGC CTCGGAGGCA 
     2281 ACTACGGAGA CTGCACTGAG AATGGCAGTG ATGTCCCTGT CAAGAACCTT TACCCCTCCA 
     2341 AGTACACACA GCAGGTGTGC ATTCACTCCT GCTTCCAGGA GAACATGATC AAGAAGTGTG 
     2401 GCTGTGCCTA CATCTTCTAC CCTAAGCCCA AGGGTGTAGA GTTCTGTGAC TACCTAAAGC 
     2461 AGAGCTCCTG GGGCTACTGC TACTATAAAC TGCAGGCTGC CTTCTCCTTG GATAGCCTGG 
     2521 GCTGCTTCTC CAAGTGCAGG AAGCCGTGCA GTGTGACCAA CTACAAGCTC TCTGCTGGCT 
     2581 ACTCAAGATG GCCGTCTGTG AAGTCCCAGG ATTGGATCTT CGAGATGCTA TCCTTGCAGA 
     2641 ATAATTACAC GATCAACAAC AAAAGAAACG GAGTTGCTAA ACTCAACATC TTCTTCAAAG 
     2701 AGCTGAACTA TAAAACTAAT TCGGAGTCTC CCTCTGTCAC GATGGTCAGC CTCCTGTCCA 
     2761 ACCTGGGCAG CCAGTGGAGC CTGTGGTTCG GCTCATCTGT GCTGTCCGTG GTGGAAATGG 
     2821 CGGAGCTCAT CTTCGACCTC CTGGTCATCA CACTCATCAT GTTACTGCAC AGGTTCCGGA 
     2881 GCCGGTACTG GTCTCCAGGA CGAGGGGCCA GGGGTGCCAG GGAGGTGGCC TCTACCCCAG 
     2941 CTTCCTCCTT CCCTTCCCGT TTCTGTCCCC ACCCTACATC CCCGCCACCT TCTTTGCCCC 
     3001 AGCAGGGCAC GACCCCTCCC CTGGCCCTGA CAGCCCCTCC ACCTGCCTAT GCTACCCTAG 
     3061 GCCCCTCTGC CTCTCCACTG GACTCGGCTG TGCCTGGCTC TTCTGCCTGT GCTCCGGCCA 
     3121 TGGCACTCAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     3181 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     3241 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     3301 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     3361 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     3421 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     3481 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     3541 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     3601 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     3661 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     3721 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     3781 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     3841 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     3901 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     3961 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     4021 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     4081 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     4141 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     4201 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     4261 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     4321 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     4381 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     4441 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     4501 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     4561 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     4621 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     4681 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     4741 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     4801 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     4861 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     4921 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     4981 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     5041 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     5101 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     5161 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     5221 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     5281 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     5341 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     5401 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     5461 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     5521 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     5581 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     5641 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     5701 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     5761 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     5821 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     5881 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     5941 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     6001 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     6061 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     6121 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     6181 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     6241 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     6301 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     6361 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     6421 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     6481 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     6541 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     6601 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     6661 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     6721 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     6781 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     6841 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     6901 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     6961 CAAAAATTTA ACGCGAATTT T
//