LOCUS       MR227032                9021 bp    DNA     circular  SYN 
DEFINITION  Map3k5 (Myc-DDK-tagged) - Mouse mitogen-activated protein kinase kinase kinase 5 (Map3k5)
ACCESSION   NM_008580
VERSION     NM_008580.4
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5178..5269 
                     /label="Myc-DDK"  
     misc_feature    5299..5884 
                     /label="PolyA signal"  
     misc_feature    5356..5375 
                     /label="Restriction Enzyme"  
     misc_feature    6160..6832 
                     /label="ColE1"  
     misc_feature    7262..8056 
                     /label="Kan/Neo"  
     misc_feature    8091..8393 
                     /label="SV40 ori"  
     misc_feature    8479..8956 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5160..5166 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5160 
                     /label="Map3k5"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGCACCGAA GCCGGCGAGG GCATCACTTT CTCCGTGCCG CCCTTTGCGT 
     1081 CCGTGGGCTT TTGCACCATC CCGGAGGGCG GCAGCTGCCG GAGGGGAGGA GGAGCGGCGA 
     1141 CGGCGGCGGA GGGAGAGCCG AGCCTGCAGC CCTTGCTTGT GCCGCCGCCG CCGCCACCGC 
     1201 CGGGCAGTTT CTGGAACGTG GAGAGCGCGG CGGCTCCCGG CACCAGCTGC CCTACCACCG 
     1261 CCCCCGGGAG CAGCGCCACC CGAGGCCGGG GCAACTCTGG CAGCGGGGGC GGCCGGCGTA 
     1321 CCACCGTGGC TTATGTGATC AATGAGGCGA GCCAGGGGCA GCTGGTGGTG GCGGAGAGCG 
     1381 AGGCCCTGCA GAGCCTGCGG GAGGCGTGCG AGGCGGTGGG CGCCACCCTG GAGACCCTGC 
     1441 ATTTCGGGAA GCTGGACTTC GGGGAAACCG CCGTGCTGGA CCGTTTTTAC AACGCAGATA 
     1501 TTGCTGTGGT GGAGATGAGC GACGCCTTCC GGCAGCCTTC CTTGTTTTAC CATCTTGGAG 
     1561 TGCGAGAAAG CTTCAGTATG GCCAACAACA TCATCCTCTA CTGCGATACT AATTCCGATT 
     1621 CACTCCAGTC CCTGAAGGAA ATTATTTGCC AGAAGAATAC TGTGTGCACC GGGAACTACA 
     1681 CCTTCATCCC CTACATGGTG ACACCACACA ACAAGGTCTA CTGCTGTGAC AGCAGCTTCA 
     1741 TGAAGGGCCT GACTGAGCTC ATGCAGCCTA ACTTCGAGCT CCTGCTGGGG CCCATCTGCT 
     1801 TACCCCTCGT GGACCGTTTC GTTCAGCTTT TGAAGGTGGC GCAAGCGAGT TCCAGCCAGT 
     1861 ACTTCCGGGA ATCCATACTC AGCGATATCA GGAAAGCACG GAACCTGTAC ACTGGTAAAG 
     1921 AATTGGCAGC TGAATTGGCG AGGATCCGGC AACGGGTAGA CAATATTGAA GTTTTGACAG 
     1981 CAGACATTGT TATCAACCTG TTGCTTTCCT ACAGAGACAT CCAGGACTAT GACTCCATAG 
     2041 TGAAGCTGGT AGAGACGTTA GAAAAACTTC CAACCTTCGA CTTGGCCTCG CACCACCACG 
     2101 TGAAGTTTCA TTACGCATTT GCACTGAACA GGAGAAATCT CCCTGGAGAC AGAGCAAAAG 
     2161 CTCTTGACAT CATGATCCCC ATGGTACAAA GTGAAGAGCA AGTTGCTTCG GATATGTATT 
     2221 GTCTCGTTGG CCGAATCTAC AAAGATATGT TTTTGGATTC AAATTTCACT GACACGGAAA 
     2281 GCAGGGACCA TGGAGCATCT TGGTTCAAAA AGGCATTTGA GTCTGAGCCA ACACTACAGT 
     2341 CAGGAATTAA TTATGCAGTT CTCTTGCTGG CGGCTGGGCA CCAGTTTGAA TCTTCTTTTG 
     2401 AGCTTCGGAA AGTTGGAGTG AAGCTGAGTA GCCTTCTGGG TAAAAAGGGG AACTTGGAAA 
     2461 AACTCCAGAG CTACTGGGAA GTCGGATTTT TTCTGGGAGC CAGTGTCCTG GCCAACGACC 
     2521 ACCTCAGGGT CATTCAGGCA TCCGAGAAGC TGTTCAGACT GAAGACACCA GCGTGGTACC 
     2581 TCAAGTCTAT CGTGGAGACC ATTCTGATAT ATAAACACTT TGTGAAACTG ACCACAGAGC 
     2641 AGCCCTCGGC TAAGCAGGAA CTCGTGGACT TCTGGATGGA TTTCCTGGTC GAGGCCACAA 
     2701 AAACAGATGT TACTGTTGTC AGGTTCCCAG TATTAATATT AGAGCCAACC AAAATCTATC 
     2761 AGCCTTCTTA CCTGTCTATC AATAATGAAG TTGAGGAGAA GACAATATCT ATTTGGCATG 
     2821 TTCTCCCTGA TGACAAGAAA GGTATACACG AGTGGAACTT CGGTGCTTCC TCTGTCAGGG 
     2881 GAGTGAGCAT CTCTAAGTTT GAAGAACGAT GCTGTTTCCT TTATGTGCTT CACAATTCTG 
     2941 ATGACTTCCA AATCTACTTC TGCACAGAAC TTCACTGTAA AAGGTTTTTT GAAATGGTGA 
     3001 ATACCATCAC GGAAGAGAAG GGGAGAGGCG CGGAGGACGG AGACTGTGAG GGTGACTCCC 
     3061 TGGAGTATGA CTACGAATAC GATGAAAATG GAGACAGAGT TGTGTTAGGG AAGGGCACTT 
     3121 ATGGGATCGT CTATGCAGGA CGAGACCTGA GCAACCAGGT CAGAATTGCT ATTAAGGAAA 
     3181 TCCCAGAGAG AGACAGCAGA TACTCTCAGC CCCTGCATGA AGAAATCGCC CTGCACAAGC 
     3241 ATCTGAAACA CAAAAACATT GTCCAGTATC TGGGCTCCTT CAGTGAGAAC GGCTTCATCA 
     3301 AAATCTTCAT GGAGCAAGTC CCAGGAGGAA GCCTCTCTGC TCTCCTTCGT TCAAAATGGG 
     3361 GCCCATTAAA GGACAACGAA CAGACGATTG GCTTCTATAC GAAGCAGATA CTGGAAGGAT 
     3421 TAAAATACCT TCACGACAAT CAGATAGTCC ACCGGGATAT AAAGGGTGAC AATGTATTGA 
     3481 TTAATACCTA CAGTGGTGTG CTCAAGATCT CTGACTTCGG GACATCCAAG AGGCTTGCTG 
     3541 GCATAAACCC ATGTACCGAA ACCTTTACAG GTACCCTTCA GTATATGGCG CCAGAAATAA 
     3601 TCGATAAAGG ACCACGAGGC TATGGAAAGG CCGCCGACAT TTGGTCACTG GGCTGCACAA 
     3661 TCATCGAAAT GGCTACTGGA AAACCACCAT TTTATGAACT AGGAGAGCCA CAGGCAGCCA 
     3721 TGTTCAAGGT GGGGATGTTT AAGGTCCACC CGGAGATCCC AGAGTCCATG TCGGCGGAGG 
     3781 CCAAGGCGTT CATACTGAAG TGTTTTGAAC CAGACCCTGA CAAGAGAGCC TGTGCTAATG 
     3841 ACTTGCTTAT TGATGAGTTC TTAAAAGTGT CCAGCAAAAA GAAAAAGACA CAACCCAAAC 
     3901 TTTCAGCTCT CTCGACTGGA TCGAATGAGT ATCTTAGAAG CATCTCCCTG CCGGTCCCTG 
     3961 TCCTGGTAGA AGACACCAGC AGCAGTAGCG AGTATGGCTC TGTTTCTCCT GATACAGAGC 
     4021 TGAAGGCGGA CCCCTTCTCC TTCAAAGCCA GAGCTAAGTC CTGTGGAGAA AAGGACGGGA 
     4081 AAGGGATACG GACATTGTTC CTGGGCATTC CAGATGAAAA TTTTGAAGAT CACAGTGCGC 
     4141 CCCCATCTCC TGAAGAGAAA GACTCGGGCT TTTTTATGCT GAGAAAGGAT AGTGAGAGGC 
     4201 GAGCTACCCT TCACAGAATC CTGACCGAAG ACCAGGACAA GGTTGTAAGG AACCTAATGG 
     4261 AATCTCTGGC CCAGGGTGCT GAAGAGCCTA AACTAAAGTG GGAACACATC ACAACCCTCA 
     4321 TCTCGAGCCT CAGAGAGTTT GTGAGGTCCA CTGACCGAAA AATCATAGCC ACTACACTGT 
     4381 CCAAGCTCAA GCTGGAGCTG GACTTCGACA GCCACGGCAT CAGCCAGGTG CAGGTGGTCC 
     4441 TCTTTGGCTT CCAGGATGCG GTCAATAAAG TTCTTCGGAA TCATAACATC AAGCCACACT 
     4501 GGATGTTTGC CCTGGACAGC ATCATCCGAA AGGCTGTGCA GACTGCAATT ACCATTCTGG 
     4561 TTCCAGAGTT GAGGCCACAT TTTAGCCTTG CATCAGAGAG CGACACAGCT GATCCGGAAG 
     4621 ACCTGGATGT CGAAGATGAA CACGAGGAAT TGTCTTCAAA TCAGACAGTC CGACGGCCTC 
     4681 AGGCCATCAC TGAGGACGCT GTGGCTACCT CGGGGGTGAG CACGCTAAGC TCCACTGTGT 
     4741 CCCATGACTC CCAGAATGCA CACCGGTCGC TGAACGTGCA GCTTGGAAGG ATGAAGATTG 
     4801 AAACTAATAG GTTACTTGAA GAGCTGGTTC GGAAAGAGAG AGAGTTACAG GCACTCCTCC 
     4861 ATCAAGCCAT TGAGGAAAAA GACCAAGAAA TTAGGCACCT GAAGCTCAAG TCCCAGCCCA 
     4921 TAGATATCCC TGGGTTTCCT GTGTGCCACC TGAACTCTCC TGGCACGACC ACGGAAGATT 
     4981 CTGAACTTCC TGGCTGGCTG AGAGAAAATG GAGCTGATGA AGACACTATA AGTCGGTTTC 
     5041 TGGCTGAGGA TTACACGCTA GTGGATGTTC TTTACTACGT CACACGTGAT GATCTGAAGT 
     5101 GCCTCAGACT AAGGGGCGGG ATGCTGTGCA CGCTGTGGAA GGCCATCATT GACTTTCGGA 
     5161 ACAAATGCAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     5221 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     5281 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     5341 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     5401 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     5461 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     5521 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     5581 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     5641 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     5701 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     5761 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     5821 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     5881 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     5941 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     6001 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     6061 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     6121 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     6181 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     6241 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     6301 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     6361 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     6421 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     6481 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     6541 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     6601 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     6661 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     6721 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     6781 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     6841 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     6901 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     6961 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     7021 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     7081 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     7141 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     7201 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     7261 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     7321 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     7381 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     7441 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     7501 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     7561 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     7621 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     7681 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     7741 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     7801 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     7861 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     7921 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     7981 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     8041 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     8101 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     8161 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     8221 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     8281 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     8341 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     8401 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     8461 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     8521 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     8581 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     8641 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     8701 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     8761 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     8821 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     8881 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     8941 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     9001 CAAAAATTTA ACGCGAATTT T
//