LOCUS       MR227017                7242 bp    DNA     circular  SYN 
DEFINITION  Itgb6 (Myc-DDK-tagged) - Mouse integrin beta 6 (Itgb6), transcript variant 2
ACCESSION   NM_021359
VERSION     NM_021359.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3399..3490 
                     /label="Myc-DDK"  
     misc_feature    3520..4105 
                     /label="PolyA signal"  
     misc_feature    3577..3596 
                     /label="Restriction Enzyme"  
     misc_feature    4381..5053 
                     /label="ColE1"  
     misc_feature    5483..6277 
                     /label="Kan/Neo"  
     misc_feature    6312..6614 
                     /label="SV40 ori"  
     misc_feature    6700..7177 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3381..3387 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3381 
                     /label="Itgb6"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGGATTGAG CTGGTCTGCC TGTTTCTGCT ACTTCTAGGA AGGAATGATC 
     1081 ACGTCCAAGG TGGCTGTGCC TGGGGTGGTG CAGAAAGCTG CTCAGACTGC CTGCTCACAG 
     1141 GACCCCACTG CGCCTGGTGC TCCCAGGAGA ATTTCACCCA CCTGTCTGGA GCTGGCGAGA 
     1201 GGTGCGACAC CCCAGCGAAT CTTTTAGCCA AAGGATGTCA ACTACCCTTC ATTGAAAACC 
     1261 CTGTCTCCCG CATAGAAGTC CTTCAAAATA AGCCTCTCAG CGTGGGCCGA CAGAAGAACA 
     1321 GTTCTGACAT TGTTCAGATT GCTCCTCAAA GCTTGGTTCT TAAATTGAGA CCAGGGCGTG 
     1381 AGCAGACTCT GCAAGTGCAG GTCCGCCAAA CTGAAGATTA CCCAGTAGAT CTGTATTACC 
     1441 TCATGGACCT CTCCGCCTCT ATGGATGACG ACCTCAACAC CATCAAAGAG CTTGGCTCCC 
     1501 GGCTGGCCAA AGAGATGTCC AAACTAACCA GCAACTTTAG ACTGGGCTTT GGCTCTTTTG 
     1561 TGGAAAAGCC CGTTTCTCCT TTTATGAAAA CAACCCCAGA GGAAATCACC AACCCTTGCA 
     1621 GTAGTATCCC CTATTTCTGC TTACCTACAT TTGGATTCAA GCACATTTTG CCATTAACTG 
     1681 ATGATGCCGA GAGATTCAAT GAAATTGTGA GGAAACAGAA GATTTCTGCT AATATTGACA 
     1741 CACCTGAAGG TGGATTTGAT GCAATCATGC AGGCTGCTGT GTGTAAGGAA AAGATTGGCT 
     1801 GGCGCAATGA CTCGCTCCAC CTCCTGGTTT TTGTGAGTGA TGCCGATTCT CATTTTGGAA 
     1861 TGGACAGCAA GCTGGCAGGC ATTGTCATTC CCAATGATGG ACTCTGTCAC TTGGACCACA 
     1921 GGAATGAATA TTCCATGTCA ACTGTCTTGG AATATCCAAC TATCGGCCAA CTCATTGATA 
     1981 AACTGGTACA AAACAACGTG CTACTGATCT TTGCAGTCAC CCAAGAACAA GTCCATCTGT 
     2041 ATGAGAACTA TGCAAAACTC ATTCCTGGAG CAACCGTGGG ACTGCTTCAG AAGGATTCTG 
     2101 GGAACATCCT TCAGCTGATC ATCTCCGCTT ATGAAGAACT GCGGTCTGAG GTGGAACTGG 
     2161 AAGTGTTAGG GGACACAGAA GGACTCAACC TGTCTTTCAC AGCTCTCTGT AACAATGGTG 
     2221 TCCTCTTCCC ACACCAAAAG AAATGCTCCC ACATGAAAGT TGGAGACACA GCATCCTTCA 
     2281 ATGTGACTGT GAGCGTATCC AACTGTGAGA AAAGAAGCAG GAACCTCATC ATAAAGCCAG 
     2341 TGGGGCTGGG GGACACCCTA GAAATACTCG TCAGTGCAGA ATGTGACTGC GACTGCCAGA 
     2401 GAGAAATAGA AACTAACAGC TCTAAGTGTC ACAATGGGAA TGGCTCCTTC CAGTGTGGGG 
     2461 TGTGTACCTG CAACCCCGGC CACATGGGTC CTCACTGCGA GTGTGGTGAG GATATGGTGA 
     2521 GCACGGATTC CTGCAAGGAG TCCCCGGGTC ACCCTTCATG CAGTGGAAGG GGTGACTGCT 
     2581 ATTGTGGGCA GTGCATCTGC CACTTATCGC CGTACGGAAG CATCTACGGA CCTTACTGCC 
     2641 AGTGTGACAA CTTCTCCTGT CTGAGACACA AAGGCCTTCT CTGTGGAGAT AACGGTGACT 
     2701 GTGACTGTGG CGAGTGTGTG TGCCGGGATG GCTGGACAGG TGAATACTGT AACTGTACAA 
     2761 CCAACAGGGA CTCGTGTACA TCTGAGGATG GAGTGCTGTG CAGTGGACGT GGGGACTGTG 
     2821 TCTGTGGCAA GTGTGTCTGC AGAAACCCTG GAGCCTCAGG ACCCACCTGT GAACGCTGTC 
     2881 CTACCTGTGG CGACCCCTGT AACTCTAAAC GGAGTTGCAT CGAGTGCTAC CTGTCTGCAG 
     2941 ATGGCCAGGC CCAAGAAGAG TGTGCTGACA AGTGTAAAGC CATTGGTGCC ACCATCAGTG 
     3001 AGGAAGATTT TTCAAAGGAT ACTTCTGTCT CCTGCTCTCT ACAAGGAGAA AATGAATGCC 
     3061 TTATTACATT CCTAATAACT ACGGATAATG AAGGGAAAAC CATCATTCAC AACATCAATG 
     3121 AAAAAGACTG CCCCAAACCT CCAAACATTC CCATGATCAT GTTGGGGGTG TCACTGGCGA 
     3181 TCCTGCTCAT CGGAGTTGTG CTACTGTGCA TTTGGAAGCT GCTGGTATCA TTTCATGACC 
     3241 GGAAGGAAGT TGCTAAATTT GAAGCAGAAC GCTCTAAGGC CAAGTGGCAA ACGGGAACCA 
     3301 ATCCTCTGTA CCGAGGTTCC ACCAGCACTT TTAAGAACGT GACCTACAAG CACAGGGAGA 
     3361 AGCACAAAGC AGGCCTTTCC TCAGATGGGA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     3421 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     3481 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     3541 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     3601 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     3661 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     3721 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     3781 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     3841 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     3901 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     3961 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     4021 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     4081 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     4141 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     4201 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     4261 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     4321 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     4381 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     4441 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     4501 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     4561 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     4621 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     4681 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     4741 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     4801 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     4861 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     4921 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     4981 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     5041 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     5101 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     5161 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     5221 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     5281 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     5341 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     5401 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     5461 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     5521 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     5581 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     5641 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     5701 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     5761 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     5821 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     5881 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     5941 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     6001 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     6061 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     6121 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     6181 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     6241 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     6301 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     6361 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     6421 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     6481 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     6541 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     6601 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     6661 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     6721 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     6781 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     6841 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     6901 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     6961 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     7021 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     7081 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     7141 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     7201 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//