LOCUS       MR226939                9276 bp    DNA     circular  SYN 
DEFINITION  Pola1 (Myc-DDK-tagged) - Mouse polymerase (DNA directed), alpha 1 (Pola1)
ACCESSION   NM_008892
VERSION     NM_008892.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5433..5524 
                     /label="Myc-DDK"  
     misc_feature    5554..6139 
                     /label="PolyA signal"  
     misc_feature    5611..5630 
                     /label="Restriction Enzyme"  
     misc_feature    6415..7087 
                     /label="ColE1"  
     misc_feature    7517..8311 
                     /label="Kan/Neo"  
     misc_feature    8346..8648 
                     /label="SV40 ori"  
     misc_feature    8734..9211 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5415..5421 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5415 
                     /label="Pola1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGCCCATG CACGAAGAGG ACTGTAAACT GGAGGCAAGC GCTGTGTCAG 
     1081 ATTCAGGGAG TTTTGCAGCT TCCCGAGCTC GGCGAGAAAA GAAATCAAAG AAAGGACGTC 
     1141 AAGAAGCTTT AGAGAGACTG AAAAAGGCTA AAGCTGGGGA GAAGTATAAA TACGAAGTTG 
     1201 AGGACCTCAC AAGTGTTTAT GAAGAAGTTG ATGAAGAGCA GTATTCGAAA CTGGTTCAGG 
     1261 CCCGTCAGGA TGATGACTGG ATTGTGGATG ACGATGGGAT AGGCTATGTG GAAGATGGCA 
     1321 GAGAGATTTT TGATGATGAC CTTGAGGATG ATGCACTTGA CACCTGTGGA AAAGGAAGTG 
     1381 ATGGCAAAGC ACACAGAAAG GACAGGAAGG ATGTAAAGAA ACCTTCAGTG ACAAAACCAA 
     1441 ACAATATCAA GGCAATGTTC ATTGCTAGTG CCGGGAAGAA AACTACAGAT AAAGCAGTAG 
     1501 ACTTGTCCAA GGATGATCTG CTAGGTGACA TTCTACAGGA TCTAAACACT GAGACAGCTC 
     1561 AAATAACTCC ACCACCTGTA TTGATACCAA AGAAAAAAAG ATCTACTGGG GCTTTGCTCA 
     1621 ACCCTTTTTC AGTGCACACC CCTAAGGCAA TTCCTTCAGG AAAACCTGCT TCCCCTGTCC 
     1681 TCAGGAATGA GCCTCTGTTA ACTCCCATTC CTCTTAAACG TGCTGAACTT GCTGGAGAGC 
     1741 TGGCTCAGCC AGAATGTCCA GAAGATGAGC AGGAGCTAGG GGTAATGGAG TTTGAAGACG 
     1801 GTGACTTTGA TGAGTCCATG GACACAGAGA AGGTAGATGA GAAGCCTGTG ACTGCTAAGA 
     1861 CTTGGGACCA AGAGACTGAG CCAGTAGAAA GGGTGGAACA TGAGGCAGAT CCTGAGAGGG 
     1921 GAACCACATC TTACTTAGAA AATTTTCTGC CAGATGTCTC TTGTTGGGAC ATTGATCAGG 
     1981 ATGATGAAAG CATACCTCAG GAAGTTCAAG TGGATTCCAG TAATCTCCCA TTGGTAAAAG 
     2041 GGGCAGATGA TGAACAAGTG TTTCAGTTTT ATTGGCTGGA TGCTTATGAA GATCCATACA 
     2101 ACCAACCAGG TGTGGTTTTT CTGTTTGGCA AAGTGTGGAT TGAATCAGTC AAAACCCATG 
     2161 TGAGCTGTTG TGTTATGGTG AAAAATATTG AGCGAACACT CTACTTCCTT CCCCGTGAAA 
     2221 TGAAATTTGA CCTAAACACA GGGAAAGAAA CAGCAATTCC AGTTACAATG AAGGATGTTT 
     2281 ATGAAGAATT TGATTCAAAA ATTTCAGCAA AATATAAAAT AATGAAGTTC AAGTCCAAGA 
     2341 TAGTGGAAAA GAACTATGCT TTTGAGATAC CTGATGTTCC AGAAAAATCT GAGTACCTGG 
     2401 AAGTTAGATA CTCGGCAGAA GTGCCACAGC TTCCACAGAA TTTAAAAGGA GAAACATTTT 
     2461 CTCATGTGTT TGGAACTAAC ACGTCTAGCT TGGAACTGTT CTTGATGAAT AGGAAGATCA 
     2521 AAGGACCTTG TTGGCTTGAA GTAAAAAATC CACAACTCTT GAATCAGCCA ATCAGTTGGT 
     2581 GTAAATTTGA GGTAATGGCT TTGAAACCAG ACCTGGTGAA TGTAATTAAG GATGTCAGTC 
     2641 CTCCTCCACT TGTGGTGATG TCTTTCAGCA TGAAGACAAT GCAGAATGTA CAGAACCATC 
     2701 AACATGAGAT TATTGCTATG GCGGCTTTGG TTCATCACAG TTTTGCATTA GATAAAGCAC 
     2761 CTCCAGAGCC TCCCTTTCAG ACACATTTCT GTGTTGTGTC TAAACCAAAG GACTGTATTT 
     2821 TCCCATGTGA TTTCAAAGAA GTGATTTCGA AAAAGAATAT GAAGGTTGAG ATCGCTGCAA 
     2881 CAGAAAGAAC CTTGATAGGA TTTTTCCTTG CAAAAGTTCA CAAAATTGAC CCTGACATTC 
     2941 TTGTGGGTCA TAATATTTGT AGTTTTGAAC TGGAAGTGCT GCTGCAAAGA ATTAATGAGT 
     3001 GCAAGGTTCC TTACTGGTCC AAGATAGGTC GGCTGAGGAG ATCCAACATG CCAAAACTTG 
     3061 GGAGCCGGAG TGGATTTGGT GAAAGAAATG CTACCTGTGG CCGAATGATA TGTGATGTGG 
     3121 AAATTTCAGC AAAAGAATTG ATTCATTGTA AAAGCTACCA TTTGTCTGAA CTTGTTCAGC 
     3181 AGATTCTGAA AACTGAGAGG ATTGTAATTC CAACAGAAAA TATACGAAAC ATGTATAGTG 
     3241 AATCTTCTTA TCTGTTGTAC CTGTTGGAAC ATATCTGGAA AGATGCCAGA TTCATTTTGC 
     3301 AGATCATGTG TGAGCTAAAT GTTCTTCCAT TAGCATTGCA GATCACTAAC ATCGCTGGGA 
     3361 ACATTATGTC TAGGACACTG ATGGGTGGAC GATCTGAGCG AAATGAGTTT TTGTTGCTTC 
     3421 ATGCATTTTA TGAAAACAAC TATATTGTGC CTGACAAGCA GATTTTCAGA AAACCTCAGC 
     3481 AAAAACTGGG AGATGAAGAT GAGGAAATTG ATGGGGACAC CAATAAATAC AAGAAAGGGC 
     3541 GTAAGAAAGC AACTTACGCT GGAGGCTTGG TTTTGGACCC CAAAGTTGGT TTTTATGACA 
     3601 AGTTCATTTT GCTCCTGGAT TTCAACAGTT TATATCCTTC CATCATTCAG GAATTTAACA 
     3661 TTTGTTTTAC AACAGTGCAA AGAGTTACTT CAGAGGTGCA GAAAGCTACA GAGGATGAGG 
     3721 AACAAGAACA GATCCCTGAG TTGCCAGATC CAAACTTAGA GATGGGCATT TTGCCCAGAG 
     3781 AGATTCGGAA ACTGGTAGAG CGGAGAAAAC AAGTCAAACA GCTAATGAAA CAGCAAGATT 
     3841 TAAATCCAGA CCTTGTTCTT CAGTATGACA TCCGACAGAA GGCTTTGAAG CTCACAGCAA 
     3901 ACAGTATGTA TGGTTGCCTG GGATTTTCGT ACAGCAGATT TTATGCTAAA CCACTGGCTG 
     3961 CCTTGGTGAC ATACAAAGGA AGGGAGATTT TGATGCATAC GAAAGACATG GTACAGAAGA 
     4021 TGAATCTTGA AGTTATTTAT GGAGACACAG ATTCAATTAT GATAAATACC AATAGCACCA 
     4081 ACCTGGAAGA AGTATTTAAG TTGGGAAACA AGGTAAAAAG TGAAGTGAAT AAATTGTACA 
     4141 AACTGCTTGA AATAGACATT GATGCTGTTT TCAAGTCTCT GCTGTTGCTG AAGAAAAAGA 
     4201 AATATGCTGC TCTAGTTGTT GAACCAACAT CAGATGGGAA TTACATTACC AAGCAGGAGC 
     4261 TGAAAGGATT AGATATAGTT AGAAGAGACT GGTGTGATCT TGCCAAGGAC ACTGGAAACT 
     4321 TTGTCATTGG CCAGATTCTT TCTGATCAAA GCCGGGACAC CATTGTGGAA AACATTCAGA 
     4381 AGAGGTTAAT AGAAATTGGA GAAAATGTGC TAAATGGCAG TGTCCCAGTG AGCCAGTTTG 
     4441 AAATTAACAA GGCATTGACA AAGGATCCCC AGGATTACCC TGATAGAAAA AGCTTACCTC 
     4501 ATGTACATGT TGCACTGTGG ATAAATTCTC AAGGAGGCAG GAAGGTGAAA GCTGGTGATA 
     4561 CGGTGTCCTA TGTTATTTGT CAGGATGGGT CAAACCTCAC TGCAACTCAG AGGGCCTATG 
     4621 CCCCAGAACA ATTGCAGAAA CTTGACAATT TAGCCATCGA CACCCAATAC TACTTGGCCC 
     4681 AGCAGATCCA CCCTGTTGTG GCTCGGATCT GTGAGCCAAT AGATGGAATT GATGCTGTAC 
     4741 TCATTGCATT ATGGCTAGGA CTTGACTCCA CTCAATTTAG AGTTCATCAG TATCACAAAG 
     4801 ATGAAGAGAA TGATGCCCTC CTTGGTGGTC CAGCACAGCT AACTGATGAA GAGAAATACA 
     4861 AGGATTGTGA AAAATTCAAA TGCCTTTGTC CTTCATGTGG AACTGAAAAT ATTTATGATA 
     4921 ATGTCTTTGA AGGCTCGGGA CTGGATATGG AACCCAGTTT GTATCGTTGC AGTAATGTTG 
     4981 ATTGTAAGGT TTCACCGTTG ACCTTCATGG TACAGCTGAG CAACAAATTG ATCATGGACA 
     5041 TTAGACGGTG CATTAAAAAG TACTATGATG GCTGGTTGAT ATGTGAAGAG CCAACTTGTT 
     5101 GCAGTCGCCT TCGACGTCTT CCCCTTCATT TCTCCCGAAA TGGGCCTCTG TGTCCAGTCT 
     5161 GCATGAAAGC TGTGCTTAGA CCAGAGTATT CTGACAAGTC CTTGTACACC CAGCTGTGCT 
     5221 TTTACCGGTA CATTTTTGAT GCGGACTGTG CACTGGAAAA ACTTACTGAA CATGAGAAAG 
     5281 ATAAATTGAA GAAGCAGTTT TTCCCTCTCC GAGTTCTTCA GGACTACAGA AAAGTCAAGA 
     5341 ACATAGCAGA GCAGTTCTTG TCCTGGAGTG GCTACTCTGA AGTGAACCTC AGCAAGCTCT 
     5401 TCGCCAATTA CGCTGGGAAG TCCACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     5461 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     5521 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     5581 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     5641 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     5701 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     5761 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     5821 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     5881 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     5941 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     6001 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     6061 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     6121 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     6181 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     6241 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     6301 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     6361 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     6421 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     6481 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     6541 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     6601 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     6661 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     6721 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     6781 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     6841 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     6901 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     6961 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     7021 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     7081 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     7141 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     7201 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     7261 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     7321 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     7381 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     7441 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     7501 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     7561 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     7621 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     7681 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     7741 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     7801 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     7861 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     7921 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     7981 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     8041 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     8101 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     8161 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     8221 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     8281 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     8341 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     8401 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     8461 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     8521 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     8581 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     8641 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     8701 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     8761 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     8821 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     8881 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     8941 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     9001 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     9061 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     9121 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     9181 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     9241 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//