LOCUS       MR226858                7563 bp    DNA     circular  SYN 
DEFINITION  Lepr (Myc-DDK-tagged) - Mouse leptin receptor (Lepr), transcript variant 3
ACCESSION   NM_001122899
VERSION     NM_001122899.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3720..3811 
                     /label="Myc-DDK"  
     misc_feature    3841..4426 
                     /label="PolyA signal"  
     misc_feature    3898..3917 
                     /label="Restriction Enzyme"  
     misc_feature    4702..5374 
                     /label="ColE1"  
     misc_feature    5804..6598 
                     /label="Kan/Neo"  
     misc_feature    6633..6935 
                     /label="SV40 ori"  
     misc_feature    7021..7498 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3702..3708 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3702 
                     /label="Lepr"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATGTGTCAG AAATTCTATG TGGTTTTGTT ACACTGGGAA TTTCTTTATG 
     1081 TGATAGCTGC ACTTAACCTG GCATATCCAA TCTCTCCCTG GAAATTTAAG TTGTTTTGTG 
     1141 GACCACCGAA CACAACCGAT GACTCCTTTC TCTCACCTGC TGGAGCCCCA AACAATGCCT 
     1201 CGGCTTTGAA GGGGGCTTCT GAAGCAATTG TTGAAGCTAA ATTTAATTCA AGTGGTATCT 
     1261 ACGTTCCTGA GTTATCCAAA ACAGTCTTCC ACTGTTGCTT TGGGAATGAG CAAGGTCAAA 
     1321 ACTGCTCTGC ACTCACAGAC AACACTGAAG GGAAGACACT GGCTTCAGTA GTGAAGGCTT 
     1381 CAGTTTTTCG CCAGCTAGGT GTAAACTGGG ACATAGAGTG CTGGATGAAA GGGGACTTGA 
     1441 CATTATTCAT CTGTCATATG GAGCCATTAC CTAAGAACCC CTTCAAGAAT TATGACTCTA 
     1501 AGGTCCATCT TTTATATGAT CTGCCTGAAG TCATAGATGA TTCGCCTCTG CCCCCACTGA 
     1561 AAGACAGCTT TCAGACTGTC CAATGCAACT GCAGTCTTCG GGGATGTGAA TGTCATGTGC 
     1621 CGGTACCCAG AGCCAAACTC AACTACGCTC TTCTGATGTA TTTGGAAATC ACATCTGCCG 
     1681 GTGTGAGTTT TCAGTCACCT CTGATGTCAC TGCAGCCCAT GCTTGTTGTG AAACCCGATC 
     1741 CACCCTTAGG TTTGCATATG GAAGTCACAG ATGATGGTAA TTTAAAGATT TCTTGGGACA 
     1801 GCCAAACAAT GGCACCATTT CCGCTTCAAT ATCAGGTGAA ATATTTAGAG AATTCTACAA 
     1861 TTGTAAGAGA GGCTGCTGAA ATTGTCTCAG CTACATCTCT GCTGGTAGAC AGTGTGCTTC 
     1921 CTGGATCTTC ATATGAGGTC CAGGTGAGGA GCAAGAGACT GGATGGTTCA GGAGTCTGGA 
     1981 GTGACTGGAG TTCACCTCAA GTCTTTACCA CACAAGATGT TGTGTATTTT CCACCCAAAA 
     2041 TTCTGACTAG TGTTGGATCG AATGCTTCTT TTCATTGCAT CTACAAAAAC GAAAACCAGA 
     2101 TTATCTCCTC AAAACAGATA GTTTGGTGGA GGAATCTAGC TGAGAAAATC CCTGAGATAC 
     2161 AGTACAGCAT TGTGAGTGAC CGAGTTAGCA AAGTTACCTT CTCCAACCTG AAAGCCACCA 
     2221 GACCTCGAGG GAAGTTTACC TATGACGCAG TGTACTGCTG CAATGAGCAG GCGTGCCATC 
     2281 ACCGCTATGC TGAATTATAC GTGATCGATG TCAATATCAA TATATCATGT GAAACTGACG 
     2341 GGTACTTAAC TAAAATGACT TGCAGATGGT CACCCAGCAC AATCCAATCA CTAGTGGGAA 
     2401 GCACTGTGCA GCTGAGGTAT CACAGGCGCA GCCTGTATTG TCCTGATAGT CCATCTATTC 
     2461 ATCCTACGTC TGAGCCCAAA AACTGCGTCT TACAGAGAGA CGGCTTTTAT GAATGTGTTT 
     2521 TCCAGCCAAT CTTTCTATTA TCTGGCTATA CAATGTGGAT CAGGATCAAC CATTCTTTAG 
     2581 GTTCACTTGA CTCGCCACCA ACGTGTGTCC TTCCTGACTC CGTAGTAAAA CCACTACCTC 
     2641 CATCTAACGT AAAAGCAGAG ATTACTGTAA ACACTGGATT ATTGAAAGTA TCTTGGGAAA 
     2701 AGCCAGTCTT TCCGGAGAAT AACCTTCAAT TCCAGATTCG ATATGGCTTA AGTGGAAAAG 
     2761 AAATACAATG GAAGACACAT GAGGTATTCG ATGCAAAGTC AAAGTCTGCC AGCCTGCTGG 
     2821 TGTCAGACCT CTGTGCAGTC TATGTGGTCC AGGTTCGCTG CCGGCGGTTG GATGGACTAG 
     2881 GATATTGGAG TAATTGGAGC AGTCCAGCCT ATACGCTTGT CATGGATGTA AAAGTTCCTA 
     2941 TGAGAGGGCC TGAATTTTGG AGAAAAATGG ATGGGGACGT TACTAAAAAG GAGAGAAATG 
     3001 TCACCTTGCT TTGGAAGCCC CTGACGAAAA ATGACTCACT GTGTAGTGTG AGGAGGTACG 
     3061 TGGTGAAGCA TCGTACTGCC CACAATGGGA CGTGGTCAGA AGATGTGGGA AATCGGACCA 
     3121 ATCTCACTTT CCTGTGGACA GAACCAGCGC ACACTGTTAC AGTTCTGGCT GTCAATTCCC 
     3181 TCGGCGCTTC CCTTGTGAAT TTTAACCTTA CCTTCTCATG GCCCATGAGT AAAGTGAGTG 
     3241 CTGTGGAGTC ACTCAGTGCT TATCCCCTGA GCAGCAGCTG TGTCATCCTT TCCTGGACAC 
     3301 TGTCACCTGA TGATTATAGT CTGTTATATC TGGTTATTGA ATGGAAGATC CTTAATGAAG 
     3361 ATGATGGAAT GAAGTGGCTT AGAATTCCCT CGAATGTTAA AAAGTTTTAT ATCCACGATA 
     3421 ATTTTATTCC CATCGAGAAA TATCAGTTTA GTCTTTACCC AGTATTTATG GAAGGAGTTG 
     3481 GAAAACCAAA GATAATTAAT GGTTTCACCA AAGATGCTAT CGACAAGCAG CAGAATGACG 
     3541 CAGGGCTGTA TGTCATTGTA CCCATAATTA TTTCCTCTTG TGTCCTACTG CTCGGAACAC 
     3601 TGTTAATTTC ACACCAGAGA ATGAAAAAGT TGTTTTGGGA CGATGTTCCA AACCCCAAGA 
     3661 ATTGTTCCTG GGCACAAGGA CTGAATTTCC AAAAGAGAAC GGACACTCTT ACGCGTACGC 
     3721 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     3781 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     3841 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     3901 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     3961 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     4021 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     4081 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     4141 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     4201 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     4261 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     4321 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     4381 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     4441 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     4501 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     4561 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     4621 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     4681 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     4741 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     4801 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     4861 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     4921 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     4981 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     5041 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     5101 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     5161 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     5221 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     5281 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     5341 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     5401 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     5461 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     5521 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     5581 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     5641 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     5701 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     5761 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     5821 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     5881 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     5941 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     6001 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     6061 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     6121 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     6181 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     6241 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     6301 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     6361 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     6421 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     6481 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     6541 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     6601 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     6661 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     6721 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     6781 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     6841 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     6901 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     6961 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     7021 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     7081 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     7141 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     7201 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     7261 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     7321 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     7381 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     7441 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     7501 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     7561 TTT
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