LOCUS       MR226855                8367 bp    DNA     circular  SYN 
DEFINITION  Lepr (Myc-DDK-tagged) - Mouse leptin receptor (Lepr), transcript variant 1
ACCESSION   NM_146146
VERSION     NM_146146.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4524..4615 
                     /label="Myc-DDK"  
     misc_feature    4645..5230 
                     /label="PolyA signal"  
     misc_feature    4702..4721 
                     /label="Restriction Enzyme"  
     misc_feature    5506..6178 
                     /label="ColE1"  
     misc_feature    6608..7402 
                     /label="Kan/Neo"  
     misc_feature    7437..7739 
                     /label="SV40 ori"  
     misc_feature    7825..8302 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4506..4512 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4506 
                     /label="Lepr"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATGTGTCAG AAATTCTATG TGGTTTTGTT ACACTGGGAA TTTCTTTATG 
     1081 TGATAGCTGC ACTTAACCTG GCATATCCAA TCTCTCCCTG GAAATTTAAG TTGTTTTGTG 
     1141 GACCACCGAA CACAACCGAT GACTCCTTTC TCTCACCTGC TGGAGCCCCA AACAATGCCT 
     1201 CGGCTTTGAA GGGGGCTTCT GAAGCAATTG TTGAAGCTAA ATTTAATTCA AGTGGTATCT 
     1261 ACGTTCCTGA GTTATCCAAA ACAGTCTTCC ACTGTTGCTT TGGGAATGAG CAAGGTCAAA 
     1321 ACTGCTCTGC ACTCACAGAC AACACTGAAG GGAAGACACT GGCTTCAGTA GTGAAGGCTT 
     1381 CAGTTTTTCG CCAGCTAGGT GTAAACTGGG ACATAGAGTG CTGGATGAAA GGGGACTTGA 
     1441 CATTATTCAT CTGTCATATG GAGCCATTAC CTAAGAACCC CTTCAAGAAT TATGACTCTA 
     1501 AGGTCCATCT TTTATATGAT CTGCCTGAAG TCATAGATGA TTCGCCTCTG CCCCCACTGA 
     1561 AAGACAGCTT TCAGACTGTC CAATGCAACT GCAGTCTTCG GGGATGTGAA TGTCATGTGC 
     1621 CGGTACCCAG AGCCAAACTC AACTACGCTC TTCTGATGTA TTTGGAAATC ACATCTGCCG 
     1681 GTGTGAGTTT TCAGTCACCT CTGATGTCAC TGCAGCCCAT GCTTGTTGTG AAACCCGATC 
     1741 CACCCTTAGG TTTGCATATG GAAGTCACAG ATGATGGTAA TTTAAAGATT TCTTGGGACA 
     1801 GCCAAACAAT GGCACCATTT CCGCTTCAAT ATCAGGTGAA ATATTTAGAG AATTCTACAA 
     1861 TTGTAAGAGA GGCTGCTGAA ATTGTCTCAG CTACATCTCT GCTGGTAGAC AGTGTGCTTC 
     1921 CTGGATCTTC ATATGAGGTC CAGGTGAGGA GCAAGAGACT GGATGGTTCA GGAGTCTGGA 
     1981 GTGACTGGAG TTCACCTCAA GTCTTTACCA CACAAGATGT TGTGTATTTT CCACCCAAAA 
     2041 TTCTGACTAG TGTTGGATCG AATGCTTCTT TTCATTGCAT CTACAAAAAC GAAAACCAGA 
     2101 TTATCTCCTC AAAACAGATA GTTTGGTGGA GGAATCTAGC TGAGAAAATC CCTGAGATAC 
     2161 AGTACAGCAT TGTGAGTGAC CGAGTTAGCA AAGTTACCTT CTCCAACCTG AAAGCCACCA 
     2221 GACCTCGAGG GAAGTTTACC TATGACGCAG TGTACTGCTG CAATGAGCAG GCGTGCCATC 
     2281 ACCGCTATGC TGAATTATAC GTGATCGATG TCAATATCAA TATATCATGT GAAACTGACG 
     2341 GGTACTTAAC TAAAATGACT TGCAGATGGT CACCCAGCAC AATCCAATCA CTAGTGGGAA 
     2401 GCACTGTGCA GCTGAGGTAT CACAGGCGCA GCCTGTATTG TCCTGATAGT CCATCTATTC 
     2461 ATCCTACGTC TGAGCCCAAA AACTGCGTCT TACAGAGAGA CGGCTTTTAT GAATGTGTTT 
     2521 TCCAGCCAAT CTTTCTATTA TCTGGCTATA CAATGTGGAT CAGGATCAAC CATTCTTTAG 
     2581 GTTCACTTGA CTCGCCACCA ACGTGTGTCC TTCCTGACTC CGTAGTAAAA CCACTACCTC 
     2641 CATCTAACGT AAAAGCAGAG ATTACTGTAA ACACTGGATT ATTGAAAGTA TCTTGGGAAA 
     2701 AGCCAGTCTT TCCGGAGAAT AACCTTCAAT TCCAGATTCG ATATGGCTTA AGTGGAAAAG 
     2761 AAATACAATG GAAGACACAT GAGGTATTCG ATGCAAAGTC AAAGTCTGCC AGCCTGCTGG 
     2821 TGTCAGACCT CTGTGCAGTC TATGTGGTCC AGGTTCGCTG CCGGCGGTTG GATGGACTAG 
     2881 GATATTGGAG TAATTGGAGC AGTCCAGCCT ATACGCTTGT CATGGATGTA AAAGTTCCTA 
     2941 TGAGAGGGCC TGAATTTTGG AGAAAAATGG ATGGGGACGT TACTAAAAAG GAGAGAAATG 
     3001 TCACCTTGCT TTGGAAGCCC CTGACGAAAA ATGACTCACT GTGTAGTGTG AGGAGGTACG 
     3061 TGGTGAAGCA TCGTACTGCC CACAATGGGA CGTGGTCAGA AGATGTGGGA AATCGGACCA 
     3121 ATCTCACTTT CCTGTGGACA GAACCAGCGC ACACTGTTAC AGTTCTGGCT GTCAATTCCC 
     3181 TCGGCGCTTC CCTTGTGAAT TTTAACCTTA CCTTCTCATG GCCCATGAGT AAAGTGAGTG 
     3241 CTGTGGAGTC ACTCAGTGCT TATCCCCTGA GCAGCAGCTG TGTCATCCTT TCCTGGACAC 
     3301 TGTCACCTGA TGATTATAGT CTGTTATATC TGGTTATTGA ATGGAAGATC CTTAATGAAG 
     3361 ATGATGGAAT GAAGTGGCTT AGAATTCCCT CGAATGTTAA AAAGTTTTAT ATCCACGATA 
     3421 ATTTTATTCC CATCGAGAAA TATCAGTTTA GTCTTTACCC AGTATTTATG GAAGGAGTTG 
     3481 GAAAACCAAA GATAATTAAT GGTTTCACCA AAGATGCTAT CGACAAGCAG CAGAATGACG 
     3541 CAGGGCTGTA TGTCATTGTA CCCATAATTA TTTCCTCTTG TGTCCTACTG CTCGGAACAC 
     3601 TGTTAATTTC ACACCAGAGA ATGAAAAAGT TGTTTTGGGA CGATGTTCCA AACCCCAAGA 
     3661 ATTGTTCCTG GGCACAAGGA CTGAATTTCC AAAAGCCTGA AACATTTGAG CATCTTTTTA 
     3721 CCAAGCATGC AGAATCAGTG ATATTTGGTC CTCTTCTTCT GGAGCCTGAA CCCATTTCAG 
     3781 AAGAAATCAG TGTCGATACA GCTTGGAAAA ATAAAGATGA GATGGTCCCA GCAGCTATGG 
     3841 TCTCCCTTCT TTTGACCACA CCAGACCCTG AAAGCAGTTC TATTTGTATT AGTGACCAGT 
     3901 GTAACAGTGC TAACTTCTCT GGGTCTCAGA GCACCCAGGT AACCTGTGAG GATGAGTGTC 
     3961 AGAGACAACC CTCAGTTAAA TATGCAACTC TGGTCAGCAA CGATAAACTA GTGGAAACTG 
     4021 ATGAAGAGCA AGGGTTTATC CATAGTCCTG TCAGCAACTG CATCTCCAGT AATCATTCCC 
     4081 CACTGAGGCA GTCTTTCTCT AGCAGCTCCT GGGAGACAGA GGCCCAGACA TTTTTCCTTT 
     4141 TATCAGACCA GCAACCCACC ATGATTTCAC CACAACTTTC ATTCTCGGGG TTGGATGAGC 
     4201 TTTTGGAACT GGAGGGAAGT TTTCCTGAAG AAAATCACAG GGAGAAGTCT GTCTGTTATC 
     4261 TAGGAGTCAC CTCCGTCAAC AGAAGAGAGA GTGGTGTGCT TTTGACTGGT GAGGCAGGAA 
     4321 TCCTGTGCAC ATTCCCAGCC CAGTGTCTGT TCAGTGACAT CAGGATCCTC CAGGAGAGAT 
     4381 GCTCACACTT TGTAGAAAAT AATTTGAGTT TAGGGACCTC TGGTGAGAAC TTTGTACCTT 
     4441 ACATGCCCCA ATTTCAAACC TGTTCCACGC ACAGTCACAA GATAATGGAG AATAAGATGT 
     4501 GTGACTTAAC TGTGACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     4561 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     4621 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     4681 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     4741 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     4801 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     4861 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     4921 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     4981 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     5041 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     5101 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     5161 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     5221 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     5281 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     5341 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     5401 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     5461 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     5521 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     5581 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     5641 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     5701 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     5761 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     5821 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     5881 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     5941 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     6001 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     6061 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     6121 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     6181 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     6241 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     6301 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     6361 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     6421 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     6481 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     6541 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     6601 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     6661 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     6721 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     6781 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     6841 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     6901 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     6961 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     7021 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     7081 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     7141 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     7201 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     7261 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     7321 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     7381 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     7441 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     7501 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     7561 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     7621 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7681 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7741 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     7801 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     7861 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     7921 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     7981 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     8041 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     8101 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     8161 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     8221 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     8281 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     8341 ATTTAACAAA AATTTAACGC GAATTTT
//