LOCUS       MR226842                7104 bp    DNA     circular  SYN 
DEFINITION  Tbx3 (Myc-DDK-tagged) - Mouse T-box 3 (Tbx3), transcript variant 1
ACCESSION   NM_011535
VERSION     NM_011535.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3261..3352 
                     /label="Myc-DDK"  
     misc_feature    3382..3967 
                     /label="PolyA signal"  
     misc_feature    3439..3458 
                     /label="Restriction Enzyme"  
     misc_feature    4243..4915 
                     /label="ColE1"  
     misc_feature    5345..6139 
                     /label="Kan/Neo"  
     misc_feature    6174..6476 
                     /label="SV40 ori"  
     misc_feature    6562..7039 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3243..3249 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3243 
                     /label="Tbx3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGCCTCTCC ATGAGAGATC CGGTTATCCC TGGGACAAGC ATGGCCTATC 
     1081 ATCCCTTCCT ACCTCACCGG GCGCCGGACT TCGCCATGAG CGCGGTACTG GGCCACCAGC 
     1141 CGCCTTTCTT CCCCGCGCTA ACGCTGCCTC CCAACGGCGC CGCGGCGCTC TCGCTCCCCG 
     1201 GAGCTCTGGC CAAGCCCATC ATGGATCAGT TAGTGGGGGC TGCTGAGACC GGCATCCCTT 
     1261 TCTCATCCCT GGGACCCCAG GCACATCTGA GGCCTCTGAA GACCATGGAA CCCGAAGAAG 
     1321 ACGTAGAAGA TGACCCTAAG GTGCACCTGG AGGCCAAGGA ACTTTGGGAC CAGTTTCACA 
     1381 AGCGGGGTAC AGAGATGGTC ATCACGAAGT CAGGAAGGCG AATGTTCCCT CCGTTTAAAG 
     1441 TGAGGTGCTC TGGACTGGAT AAAAAGGCCA AGTATATTTT ATTGATGGAC ATTATAGCTG 
     1501 CTGACGACTG TCGATATAAA TTTCACAACT CTCGGTGGAT GGTGGCCGGT AAGGCAGACC 
     1561 CCGAAATGCC AAAGAGAATG TATATACACC CGGACAGCCC CGCTACGGGG GAGCAATGGA 
     1621 TGTCCAAAGT CGTCACTTTC CACAAACTGA AACTCACCAA CAACATATCG GATAAACACG 
     1681 GATTTACTTT GGCCTTCCCA AGCGATCACG CAACGTGGCA GGGGAATTAT AGTTTTGGGA 
     1741 CCCAGACTAT ACTAAACTCT ATGCATAAGT ACCAGCCGCG GTTCCACATC GTCAGAGCCA 
     1801 ACGATATCCT GAAACTGCCT TACAGTACTT TTCGAACCTA CCTGTTCCCG GAAACAGAAT 
     1861 TCATCGCCGT TACTGCCTAT CAGAATGACA AGATAACTCA GTTAAAAATA GACAACAATC 
     1921 CCTTTGCAAA GGGTTTTCGA GACACTGGCA ATGGCAGGAG AGAAAAAAGA AAGCAGCTCA 
     1981 CACTGCAGTC CATGAGAGTG TTTGAGGAGA GGCATAAGAA GGAGACTTCG GATGAGTCCT 
     2041 CCAGCGAGCA GGCAGCCTTC AACTGCTTTG CCCAGGCATC CTCTCCTGCT GTCTCCATCG 
     2101 TGGGGACATC CAACCTCAAA GATCTGTGTC CCAGCGAAGC CGAGAGTGAC GCCGAGGCTG 
     2161 AGAGCAAGGA GGAGCACGGC CCTGAGGCCT GCGACGCGGC CAAGATTTCC ACCACTACGG 
     2221 CCGAGGAGCC AGGCCGCGAC AAGGGCAGCC CAGCAACCAG GGCGCAGCTG TTCCCCGCGG 
     2281 AGCCCAGCCG GGCCCGCGAC ACCGCGCGTC TGGACAAGGC ATCGCCAGAC TCGCGCCATA 
     2341 GCCCGGCCAC CATCTCGTCC AGCACGCGAG TTCCGGGAGC TGATGAGCGC AGGAGTCCCG 
     2401 GGCGCGAGGG TCCAGTCGCC ACCAAGGTGG ATGAGGCGCG CGCGATTCCT GCCAAGGACG 
     2461 CCTTCGCTCC ACTGTCGGTG CAGACAGACG CCACCGCGCA CCTAGCGCAG GGGCCCCTCC 
     2521 CGGGCTTGGG TTTCGCCCCA GGCCTTGCGG GCCAGCAGTT CTTCAACGGG CACCCGCTCT 
     2581 TCCTGCACCC GGGCCAGTTC GCCATGGGCG GCGCCTTCTC TAGCATGGCT GCAGGCATGG 
     2641 GGCCCCTGCT GGCCACAGTA TCCGGAGCAT CCACCGGCGT CTCAGGCCTA GAATCCACAG 
     2701 CCATGGCCTC GGCTGCCGCA GCGCAGGGAC TGTCTGGGGC GTCGGCTGCC ACCCTGCCCT 
     2761 TCCACCTCCA ACAACACGTT CTGGCCTCCC AGGGCTTGGC TATGTCGCCT TTCGGGAGCC 
     2821 TGTTTCCTTA CCCCTACACA TACATGGCTG CAGCAGCTGC AGCCTCCACC GCGGCCGCCT 
     2881 CCAGCTCCGT GCACCGCCAC CCGTTCCTCA ATTTGAACAG CATGCGCCCC AGGCTGCGTT 
     2941 ACAGCCCCTA TTCCATCCCC GTCCCAGTGC CGGATAGCAG CAGTTTGCTG GCCACAGCTT 
     3001 TACCATCAAT GGCTTCCGCC GCGGGGCCCC TAGACGGCAA AGCGGCCGCC CTGGCAGCCA 
     3061 GCCCAGCCTC GGTGGCTGTG GACTCGGGGT CGGAACTGAA CAGCCGCTCG TCCACGCTGT 
     3121 CCTCTGGCTC AGTGTCCTTG TCACCCAAAC TCTGCTCCGA GAAGGAGGCG GCTACCAGCG 
     3181 AACTGCAGAG TATCCAGCGG CTGGTCAGTG GCTTGGAAGC CAAGCCAGAC AGGTCTTGCA 
     3241 GCGGGTCCCC TACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     3301 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     3361 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     3421 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     3481 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     3541 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     3601 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     3661 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     3721 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     3781 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     3841 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     3901 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     3961 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     4021 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     4081 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     4141 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     4201 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     4261 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     4321 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     4381 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     4441 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     4501 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     4561 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     4621 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     4681 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     4741 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     4801 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     4861 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     4921 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     4981 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     5041 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     5101 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     5161 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     5221 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     5281 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     5341 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     5401 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     5461 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     5521 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     5581 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     5641 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     5701 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     5761 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     5821 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     5881 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     5941 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     6001 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     6061 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     6121 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     6181 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     6241 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     6301 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     6361 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     6421 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     6481 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     6541 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     6601 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     6661 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     6721 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     6781 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     6841 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     6901 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     6961 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     7021 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     7081 TAACAAAAAT TTAACGCGAA TTTT
//