LOCUS       MR226808                8622 bp    DNA     circular  SYN 
DEFINITION  Jag2 (Myc-DDK-tagged) - Mouse jagged 2 (Jag2)
ACCESSION   NM_010588
VERSION     NM_010588.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4779..4870 
                     /label="Myc-DDK"  
     misc_feature    4900..5485 
                     /label="PolyA signal"  
     misc_feature    4957..4976 
                     /label="Restriction Enzyme"  
     misc_feature    5761..6433 
                     /label="ColE1"  
     misc_feature    6863..7657 
                     /label="Kan/Neo"  
     misc_feature    7692..7994 
                     /label="SV40 ori"  
     misc_feature    8080..8557 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4761..4767 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4761 
                     /label="Jag2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCGGGCACGC GGCTGGGGAC GCCTGCCTCG GCGGCTGCTG CTGCTACTGG 
     1081 TTCTGTGCGT GCAGGCGACG CGGCCCATGG GCTATTTCGA GCTGCAGCTG AGCGCGCTGC 
     1141 GGAACGTGAA CGGGGAGCTG CTGAGCGGCG CCTGCTGTGA CGGCGACGGC CGGACGACGC 
     1201 GCGCGGGGGG CTGCGGCCGC GACGAGTGCG ACACGTACGT GCGCGTGTGC CTTAAGGAGT 
     1261 ACCAGGCCAA GGTGACGCCC ACGGGGCCCT GCAGCTACGG CTACGGCGCC ACGCCCGTGC 
     1321 TGGGTGGCAA CTCCTTCTAC CTGCCGCCGG CGGGCGCTGC GGGGGACCGA GCGCGCGCGC 
     1381 GGTCTCGGAC CGGCGGCCAC CAGGACCCGG GCCTCGTCGT CATTCCCTTT CAGTTCGCCT 
     1441 GGCCGCGTTC TTTCACCCTC ATCGTGGAGG CCTGGGACTG GGACAATGAC ACCACTCCAG 
     1501 ATGAGGAGCT GCTGATTGAG CGGGTGTCGC ACGCTGGCAT GATCAACCCC GAGGACCGCT 
     1561 GGAAGAGCCT GCACTTCAGC GGCCACGTGG CACACCTGGA GCTGCAGATC CGAGTACGCT 
     1621 GTGATGAGAA CTACTACAGT GCCACCTGCA ACAAGTTCTG CCGGCCCCGC AACGACTTCT 
     1681 TTGGCCACTA TACCTGCGAC CAGTACGGCA ACAAGGCCTG CATGGATGGC TGGATGGGCA 
     1741 AAGAATGCAA AGAAGCCGTG TGTAAACAAG GATGTAATTT GCTCCACGGG GGATGCACTG 
     1801 TGCCTGGGGA GTGCAGGTGC AGCTACGGCT GGCAGGGCAA GTTCTGTGAC GAGTGTGTCC 
     1861 CCTACCCTGG CTGCGTGCAT GGCAGCTGTG TGGAGCCCTG GCACTGTGAC TGTGAGACCA 
     1921 ACTGGGGTGG CCTGCTCTGT GACAAAGACC TGAACTACTG TGGCAGCCAC CACCCCTGTG 
     1981 TCAACGGGGG TACCTGCATC AATGCTGAGC CTGACCAATA CCTCTGCGCC TGCCCAGATG 
     2041 GCTACTTGGG CAAGAACTGC GAGCGGGCTG AGCACGCCTG TGCCTCCAAC CCGTGTGCCA 
     2101 ATGGGGGCTC TTGCCACGAA GTGCCATCTG GCTTTGAATG CCACTGTCCG TCAGGATGGA 
     2161 ACGGACCCAC CTGTGCGCTC GACATTGATG AGTGTGCCTC TAACCCATGT GCAGCGGGTG 
     2221 GTACCTGCGT GGATCAGGTG GACGGCTTCG AGTGCATCTG CCCGGAGCAG TGGGTGGGGG 
     2281 CTACTTGCCA GCTGGACGCC AATGAGTGTG AAGGGAAGCC GTGCCTTAAT GCTTTTTCTT 
     2341 GCAAAAACCT GATTGGCGGC TATTACTGTG ATTGCCTCCC GGGCTGGAAG GGCATCAACT 
     2401 GCCAAATCAA CATCAACGAT TGCCATGGGC AGTGTCAGCA TGGGGGCACC TGCAAGGACC 
     2461 TGGTCAATGG GTACCAGTGT GTGTGCCCGC GGGGCTTTGG AGGTCGCCAT TGCGAACTAG 
     2521 AGTACGACAA GTGTGCCAGC AGCCCCTGCC GCCGGGGTGG CATCTGTGAG GACCTGGTGG 
     2581 ATGGCTTCCG CTGCCACTGC CCACGGGGCC TCTCTGGGCT GCACTGTGAG GTGGACATGG 
     2641 ATCTCTGTGA ACCAAGCCCC TGCCTCAACG GTGCTCGCTG CTACAACCTT GAGGGTGACT 
     2701 ACTACTGCGC CTGCCCAGAA GACTTTGGTG GCAAGAACTG CTCAGTGCCC AGGGACACAT 
     2761 GCCCTGGCGG GGCATGTAGA GTGATCGATG GCTGCGGGTT CGAGGCAGGG TCCAGGGCAC 
     2821 GCGGTGTCGC ACCCTCTGGT ATATGTGGCC CTCACGGGCA CTGCGTTAGC CTGCCTGGGG 
     2881 GAAACTTCTC CTGCATCTGT GACAGCGGCT TCACAGGCAC CTACTGCCAT GAAAACATTG 
     2941 ACGACTGCAT GGGCCAGCCC TGCCGCAACG GGGGCACGTG CATTGACGAA GTGGACTCCT 
     3001 TCCGCTGCTT CTGCCCCAGT GGCTGGGAAG GAGAACTCTG TGACATCAAT CCCAACGACT 
     3061 GCCTCCCCGA CCCCTGCCAC AGCCGCGGCC GCTGCTATGA CCTGGTCAAT GACTTCTACT 
     3121 GTGCCTGTGA CGATGGCTGG AAGGGCAAGA CCTGCCACTC ACGCGAGTTC CAGTGTGACG 
     3181 CCTACACCTG CAGCAACGGT GGCACATGCT ATGACAGCGG CGACACCTTC CGCTGCGCGT 
     3241 GCCCTCCGGG CTGGAAGGGC AGCACCTGCA CCATCGCCAA GAACAGCAGC TGTGTGCCCA 
     3301 ATCCCTGTGT GAATGGAGGC ACCTGCGTGG GCAGCGGAGA CTCTTTCTCC TGCATCTGCC 
     3361 GGGATGGCTG GGAGGGCCGC ACCTGCACAC ATAACACCAA TGACTGCAAC CCTCTGCCCT 
     3421 GCTATAACGG AGGCATCTGT GTTGATGGCG TCAACTGGTT CCGCTGCGAG TGTGCGCCTG 
     3481 GCTTTGCGGG TCCTGACTGC CGTATCAACA TTGATGAGTG CCAGTCCTCG CCCTGTGCCT 
     3541 ACGGAGCCAC GTGTGTGGAT GAGATCAACG GGTACCGCTG CAGCTGCCCA CCAGGTCGTT 
     3601 CTGGCCCCAG GTGCCAGGAA GTGGTCATAT TCACGAGGCC CTGCTGGTCC CGGGGAATGT 
     3661 CCTTCCCGCA TGGGAGTTCC TGGATGGAAG ACTGCAACAG CTGCCGCTGC CTGGATGGCC 
     3721 ACCGGGATTG TAGCAAGGTA TGGTGCGGAT GGAAGCCTTG CCTGCTCTCT GGTCAGCCCA 
     3781 GCGATCCGAG TGCCCAGTGC CCCCCAGGGC AGCAATGTCA GGAGAAGGCC GTGGGTCAGT 
     3841 GCTTGCAGCC ACCCTGTGAG AACTGGGGGG AGTGTACAGC GGAGGAGCCT CTGCCACCCA 
     3901 GCACCCCCTG TCAGCCACGG AGCAGTCATT TGGACAACAA CTGTGCCCGA CTCACACTGC 
     3961 GCTTCAACCG TGATCAAGTG CCTCAGGGCA CCACCGTGGG CGCTATCTGC TCTGGAATCC 
     4021 GAGCCTTGCC TGCCACGAGG GCGGCGGCAC ACGACCGCCT CCTCCTGCTG CTTTGTGATC 
     4081 GAGCATCCTC GGGGGCCAGT GCTGTGGAGG TGGCTGTGTC TTTCAGCCCT GCAAGGGACC 
     4141 TGCCTGACAG CAGCCTGATC CAGAGCACAG CCCACGCCAT CGTGGCTGCT ATCACTCAGA 
     4201 GAGGAAATAG CTCACTGCTG CTGGCTGTCA CCGAGGTCAA GGTGGAAACA GTTGTTATGG 
     4261 GTGGCTCTTC CACAGGTCTG TTGGTGCCCG TGCTGTGCAG CGTGTTCAGT GTGCTGTGGC 
     4321 TCGCCTGTGT GGTTATCTGC GTATGGTGGA CACGAAAGCG CAGGAAAGAA CGTGAGAGGA 
     4381 GCCGGCTACC ACGGGATGAG AGCGCCAACA ACCAGTGGGC CCCGCTCAAT CCCATCCGCA 
     4441 ACCCCATTGA GCGGCCAGGC GGCAGCGGTC TGGGAACTGG GGGCCACAAG GACATACTCT 
     4501 ACCAGTGCAA AAACTTCACA CCGCCGCCCC GCAGGGCAGG CGAGGCACTG CCCGGGCCAG 
     4561 CTGGCCATGG GGCTGGTGGG GAGGACGAGG AGGATGAAGA GCTGAGCCGT GGAGATGGGG 
     4621 ACTCCCCAGA GGCAGAGAAG TTCATCTCAC ACAAGTTCAC CAAAGACCCC AGCTGCTCCC 
     4681 TCGGAAGGCC AGCCCGCTGG GCTCCAGGGC CCAAAGTGGA CAACCGCGCC GTCAGAAGTA 
     4741 CCAAGGACGT GCGCCGTGCT GGCAGGGAAA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     4801 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     4861 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     4921 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     4981 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     5041 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     5101 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     5161 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     5221 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     5281 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     5341 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     5401 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     5461 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     5521 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     5581 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     5641 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     5701 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     5761 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     5821 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     5881 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     5941 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     6001 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     6061 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     6121 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     6181 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     6241 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     6301 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     6361 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     6421 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     6481 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     6541 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     6601 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     6661 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     6721 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     6781 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     6841 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     6901 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     6961 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     7021 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     7081 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     7141 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     7201 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     7261 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     7321 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     7381 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     7441 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     7501 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     7561 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     7621 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     7681 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     7741 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     7801 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     7861 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7921 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7981 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     8041 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     8101 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     8161 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     8221 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     8281 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     8341 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     8401 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     8461 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     8521 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     8581 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//