LOCUS       MR226653                8487 bp    DNA     circular  SYN 
DEFINITION  Nos3 (Myc-DDK-tagged) - Mouse nitric oxide synthase 3, endothelial cell (Nos3)
ACCESSION   NM_008713
VERSION     NM_008713.4
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4644..4735 
                     /label="Myc-DDK"  
     misc_feature    4765..5350 
                     /label="PolyA signal"  
     misc_feature    4822..4841 
                     /label="Restriction Enzyme"  
     misc_feature    5626..6298 
                     /label="ColE1"  
     misc_feature    6728..7522 
                     /label="Kan/Neo"  
     misc_feature    7557..7859 
                     /label="SV40 ori"  
     misc_feature    7945..8422 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4626..4632 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4626 
                     /label="Nos3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGCAACTTG AAGAGTGTGG GCCAGGAGCC TGGGCCACCC TGTGGCCTAG 
     1081 GGCTCGGGCT GGGTTTAGGG CTGTGCGGCA AGCAGGGCCC AGCCTCTCCA GCACCGGAGC 
     1141 CTAGCCAGGC GCCAGCACCC CCGTCCCCAA CCCGACCAGC ACCAGACCAC AGCCCCCCGC 
     1201 TAACCCGGCC CCCAGACGGA CCCAGGTTTC CTCGAGTAAA GAATTGGGAA GTGGGCAGCA 
     1261 TCACCTACGA CACCCTCAGT GCCCAGGCTC AGCAGGATGG GCCCTGTACC TCAAGACGCT 
     1321 GCTTGGGATC CCTGGTGTTT CCAAGGAAGT TACAGAGCCG GCCCACCCAG GGCCCTTCAC 
     1381 CCACTGAGCA GCTATTGGGT CAAGCCCGGG ACTTCATCAA TCAGTACTAT AACTCCATCA 
     1441 AAAGGAGTGG CTCCCAGGCT CATGAGCAGC GGCTTCAGGA AGTGGAGGCT GAGGTGGCAG 
     1501 CCACAGGCAC CTACCAGCTC CGGGAGAGCG AGCTGGTGTT TGGGGCCAAG CAGGCCTGGC 
     1561 GCAATGCTCC CCGCTGTGTG GGCCGGATCC AGTGGGGAAA GCTGCAGGTA TTTGATGCTC 
     1621 GGGACTGCAG GACTGCACAG GAAATGTTCA CCTACATCTG TAACCACATT AAATACGCAA 
     1681 CAAATAGAGG CAATCTTCGT TCAGCCATCA CAGTGTTCCC CCAGCGCTGC CCTGGCCGGG 
     1741 GAGACTTCCG GATCTGGAAC AGCCAGCTGA TACGCTATGC GGGCTATAGG CAGCAGGATG 
     1801 GCTCCGTGCG AGGGGACCCC GCCAACGTGG AGATCACTGA GCTCTGTATC CAACATGGCT 
     1861 GGACCCCAGG AAATGGCCGC TTTGATGTGC TGCCCCTGTT ACTCCAGGCT CCTGATGAGC 
     1921 CCCCAGAACT CTTCACTCTG CCCCCAGAGA TGGTCCTCGA GGTGCCTCTG GAGCACCCCA 
     1981 CGCTCGAGTG GTTTGCTGCC CTTGGCCTGC GCTGGTATGC CCTCCCAGCT GTGTCCAACA 
     2041 TGCTGCTAGA AATCGGGGGC CTGGAGTTTC CTGCTGCCCC TTTCAGCGGC TGGTACATGA 
     2101 GTTCAGAGAT TGGCATGAGG GACCTGTGTG ACCCTCACCG CTACAACATA CTTGAGGATG 
     2161 TGGCTGTGTG CATGGATCTG GACACCAGGA CAACCTCATC CCTGTGGAAA GACAAGGCAG 
     2221 CGGTGGAAAT TAATGTGGCC GTGTTGCACA GTTACCAGCT GGCCAAAGTG ACCATAGTGG 
     2281 ACCACCACGC CGCCACAGCC TCCTTCATGA AGCACCTGGA AAATGAGCAG AAGGCCAGAG 
     2341 GGGGCTGCCC TGCCGATTGG GCCTGGATTG TGCCCCCCAT CTCAGGCAGC CTAACTCCTG 
     2401 TCTTCCATCA AGAGATGGTC AACTATTTCC TGTCCCCTGC CTTCCGCTAC CAGCCAGACC 
     2461 CCTGGAAGGG AAGTGCAGCA AAGGGGGCAG GCATCACCAG GAAGAAGACC TTTAAGGAAG 
     2521 TAGCCAATGC AGTGAAGATC TCTGCCTCAC TCATGGGCAC GGTGATGGCG AAGCGTGTGA 
     2581 AGGCAACCAT TCTGTATGGC TCTGAGACTG GCCGGGCCCA GAGCTACGCA CAGCAGCTGG 
     2641 GAAGACTCTT CCGGAAGGCG TTTGATCCCC GGGTCCTGTG CATGGATGAG TATGATGTGG 
     2701 TGTCCCTAGA GCACGAGGCA CTGGTGTTGG TGGTGACCAG CACATTTGGC AATGGGGATC 
     2761 CTCCGGAGAA TGGAGAGAGC TTTGCAGCAG CGCTCATGGA AATGTCAGGC CCGTACAACA 
     2821 GCTCCCCTAG GCCTGAGCAG CACAAGAGCT ACAAAATCCG ATTCAACAGT GTCTCCTGCT 
     2881 CAGACCCACT GGTATCCTCT TGGCGGCGCA AGAGGAAGGA GTCTAGCAAC ACAGACAGTG 
     2941 CAGGAGCCCT GGGCACCCTC AGGTTCTGTG TGTTTGGGCT GGGCTCCCGA GCATACCCCC 
     3001 ACTTCTGTGC CTTTGCTCGA GCGGTGGACA CAAGGCTGGA GGAGCTGGGC GGGGAGCGAC 
     3061 TACTGCAGCT GGGCCAAGGT GATGAGCTCT GTGGCCAGGA GGAGGCTTTC CGAGGCTGGG 
     3121 CCCAGGCCGC CTTCCAGGCT GCCTGTGAAA CCTTCTGTGT GGGAGAAGAT GCCAAAGCTG 
     3181 CTGCCCGAGA TATCTTCAGC CCCAAACGCA GCTGGAAGCG CCAGAGGTAC CGGCTGAGTA 
     3241 CCCAGGCTGA GAGCCTGCAA TTACTACCAG GGCTGACTCA CGTGCACAGG CGGAAGATGT 
     3301 TCCAGGCTAC AATCCTCTCT GTGGAAAACC TACAGAGCAG CAAATCCACC CGAGCCACGA 
     3361 TCCTGGTGCG TCTGGACACC GGAGGCCAGG AGGGACTGCA GTACCAGCCA GGGGACCACA 
     3421 TAGGTGTGTG CCCACCCAAC CGTCCTGGCC TAGTGGAGGC ACTGCTGAGC CGAGTGGAGG 
     3481 ACCCTCCGCC ATCCACAGAA CCTGTGGCTG TGGAACAACT GGAGAAAGGC AGCCCTGGTG 
     3541 GCCCTCCCCC CGGCTGGGTA CGGGACCCCC GGCTACCCCC ATGTACGCTG CGGCAGGCTC 
     3601 TCACCTACTT CCTGGACATC ACTTCCCCGC CTAGTCCTCG CCTCCTTCGA CTGCTCAGCA 
     3661 CCCTGGCAGA AGAGTCCAGC GAACAGCAGG AGCTAGAGGC TCTCAGCCAG GACCCCCGGC 
     3721 GCTACGAAGA ATGGAAGTGG TTCAGCTGCC CCACACTGCT AGAGGTGCTG GAGCAATTTC 
     3781 CTTCAGTGGC ACTGCCTGCC CCGCTGATCC TCACCCAGCT GCCCTTGCTC CAGCCCCGGT 
     3841 ACTACTCTGT CAGTTCAGCA CCCAGCGCCC ACCCAGGAGA GATCCACCTC ACCATAGCTG 
     3901 TGCTGGCTTA CAGAACCCAG GATGGGCTGG GCCCTCTGCA CTACGGTGTC TGCTCCACGT 
     3961 GGATGAGCCA GCTCAAGGCG GGAGATCCAG TGCCCTGCTT CATCAGGGGG GCTCCCTCCT 
     4021 TCCGGCTGCC ACCTGATCCT AACTTGCCCT GCATCCTGGT GGGCCCAGGG ACTGGCATTG 
     4081 CACCCTTCCG GGGATTCTGG CAAGACAGAC TACACGACAT TGAGATCAAA GGGCTACAAC 
     4141 CTGCCCCCAT GACTTTGGTG TTTGGCTGCC GATGCTCCCA ACTGGACCAT CTCTACCGGG 
     4201 ACGAGGTACT GGACGCCCAG CAGCGTGGGG TGTTTGGACA AGTCCTCACC GCCTTTTCCA 
     4261 GGGATCCTGG CAGCCCCAAG ACCTACGTGC AAGACCTCCT GAGGACAGAG CTAGCCGCGG 
     4321 AGGTTCACCG TGTGCTGTGC CTTGAGCAAG GACATATGTT TGTCTGCGGC GATGTCACTA 
     4381 TGGCAACCAG CGTCCTGCAA ACCGTGCAGA GAATTCTGGC AACAGAGGGC GGCATGGAGC 
     4441 TGGATGAAGC CGGTGACGTC ATCGGCGTGC TGCGGGATCA GCAACGCTAC CACGAGGACA 
     4501 TTTTCGGACT CACATTGCGC ACCCAGGAGG TGACAAGCCG CATACGCACC CAGAGCTTTT 
     4561 CTTTGCAGGA GCGACAGCTG AGGGGCGCAG TGCCCTGGTC CTTTGACCCG CCTGGCCCAG 
     4621 AAATACCTGG TTCCACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     4681 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     4741 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     4801 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     4861 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     4921 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     4981 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     5041 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     5101 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     5161 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     5221 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     5281 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     5341 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     5401 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     5461 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     5521 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     5581 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     5641 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     5701 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     5761 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     5821 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     5881 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     5941 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     6001 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     6061 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     6121 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     6181 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     6241 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     6301 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     6361 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     6421 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     6481 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     6541 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     6601 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     6661 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     6721 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     6781 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     6841 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     6901 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     6961 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     7021 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     7081 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     7141 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     7201 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     7261 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     7321 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     7381 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     7441 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     7501 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     7561 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     7621 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     7681 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     7741 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7801 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7861 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     7921 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     7981 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     8041 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     8101 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     8161 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     8221 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     8281 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     8341 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     8401 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     8461 ATTTAACAAA AATTTAACGC GAATTTT
//