LOCUS       MR226578                9309 bp    DNA     circular  SYN 
DEFINITION  Magi3 (Myc-DDK-tagged) - Mouse membrane associated guanylate kinase, WW and PDZ domain containing 3 (Magi3), transcript variant 1
ACCESSION   NM_001159354
VERSION     NM_001159354.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5466..5557 
                     /label="Myc-DDK"  
     misc_feature    5587..6172 
                     /label="PolyA signal"  
     misc_feature    5644..5663 
                     /label="Restriction Enzyme"  
     misc_feature    6448..7120 
                     /label="ColE1"  
     misc_feature    7550..8344 
                     /label="Kan/Neo"  
     misc_feature    8379..8681 
                     /label="SV40 ori"  
     misc_feature    8767..9244 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5448..5454 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5448 
                     /label="Magi3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCGAAGACG TTGAAGAAGA AGAAGCACTG GCTCAGCAAG GTGCAGGAGT 
     1081 GTGCGGTATC CTGGGCCGGG CCCCCGGGCG ACTTGGGCGC CGAGATCCGC GGCGGCGCCG 
     1141 AGCGCGGCGA GTTCCCTTAC CTGGGGCGGC TCCGCGACGA GGCTGGCGGC GGCGGAGGCA 
     1201 CCTGCTGCGT GGTCTCGGGC AAGGCGCCCA GTCCGGGCGA TGTGCTGTTG GAGGTGAACG 
     1261 GGACGCCGGT CAGTGGGCTC ACCAACCGGG ACACTCTGGC TGTCATCCGC CACTTCCGCG 
     1321 AGCCCATCCG TCTGAAGACG GTGAAGCCAG GCAAAGTTAT AAATAAAGAT TTGCGACACT 
     1381 ACCTGAGTCT TCAGTTTCAG AAAGGATCAA TTGACCACAA ACTACAGCAA GTCATCAGAG 
     1441 ATAATCTCTA TTTGAGAACC ATCCCATGCA CTACAAGGGC TCCCAGGGAT GGGGAAGTCC 
     1501 CAGGGGTGGA TTATAACTTC ATTTCTGTTG AACAATTCAA GGCACTGGAA GAAAGCGGAG 
     1561 CATTGTTAGA AAGTGGAACG TATGATGGAA ATTTCTATGG CACACCCAAG CCTCCAGCAG 
     1621 AGCCTAGTCC TTTCCAGCCC GATCCAGTTG ATCAGGTCCT CTTTGACAAT GAGTTTGACA 
     1681 CAGAATCCCA AAGAAAAAGA ACCACATCTG TCAGCAAGAT GGAAAGGATG GACAGCTCTC 
     1741 TCCCTGAAGA AGAAGAAGAT GAGGACAAAG AAGCTGTTAA TGGCAGCGGA AGCATGGAAA 
     1801 CTAGAGAGAT GCATTCTGAG ACATCTGACT GCTGGATGAA GACTGTTCCA AGTTATAACC 
     1861 AAACAAATAG CTCCATGGAC TTTAGAAATT ATATGATGAG AGATGAGAAT CTGGAACCGC 
     1921 TGCCCAAAAA CTGGGAAATG GCCTACACTG ACACAGGAAT GATCTACTTC ATTGATCATA 
     1981 ATACGAAGAC AACCACCTGG TTGGATCCTC GTCTCTGCAA GAAAGCCAAA GCCCCTGAAG 
     2041 ACTGTGAAGA TGGAGAGCTT CCCTATGGCT GGGAGAAAAT AGAGGACCCT CAGTATGGAA 
     2101 CATACTACGT TGATCACCTC AACCAGAAAA CTCAGTTTGA AAATCCAGTG GAGGAAGCCA 
     2161 AGAGGAAAAA GCAGTTAGGA CAGGCTGAAA TTCATTCTGC AAAAACAGAT GTGGAAAGAG 
     2221 CACACTTTAC TCGGGACCCA TCCCAACTTA AAGGTGTACT TGTTCGAGCA TCGCTGAAAA 
     2281 AAAGCACCAT GGGCTTTGGC TTTACCATTA TTGGTGGAGA TAGGCCTGAT GAATTTCTAC 
     2341 AAGTAAAAAA CGTGCTCAAA GATGGTCCTG CCGCTCAGGA TGGGAAAATT GCACCAGGTG 
     2401 ATGTTATTGT AGACATCAAT GGCAACTGTG TCCTTGGTCA CACCCATGCA GATGTTGTCC 
     2461 AGATGTTTCA ACTGGTACCT GTCAACCAGT ATGTCAACCT TACTTTATGC CGTGGTTATC 
     2521 CACTTCCTGA TGACAGTGAA GATCCTGTTG TGGACATTGT TGCTGCTACC CCTGTCATCA 
     2581 ATGGACAATC TTTAACAAAG GGAGAGACAT GCATGAATAC TCAGGATTTT AAACTGGGAG 
     2641 CAATGGTTTT GGATCAGAAT GGAAAATCAG GACAAATCTT GGCCAGTGAT CGTCTCAATG 
     2701 GTCCATCTGA GTCAAGTGAG CAGAGGGCAT CTCTGGCATC ATCAGGCAGC TCACAGCCTG 
     2761 AACTAGTGAC TATCCCTCTG ATTAAGGGCC CCAAAGGCTT TGGGTTTGCA ATTGCTGACA 
     2821 GCCCAACTGG ACAGAAGGTG AAAATGATAT TGGATAGCCA GTGGTGTCAA GGCCTTCAAA 
     2881 AAGGGGATAT CATCAAAGAA ATTTACCATC AGAATGTGCA GAATTTAACA CATCTCCAAG 
     2941 TCGTAGAAGT CCTAAAGCAG TTCCCAGTAG GTGCAGATGT TCCATTGCTT ATCTTAAGAG 
     3001 GAGGTCCTTG TTCACCAACT AAAACTGCCA AAACGAAAAC AGATACAAAG GAAAATTCAG 
     3061 GAAGTTTGGA GACTATAAAT GAGCCTATTC CCCAGCCTAT GCCTTTTCCG CCCAGCATAA 
     3121 TCAGATCAGG ATCCCCAAAA TTGGATCCTT CTGAGGTCTA CCTGAAATCT AAGACTTTAT 
     3181 ATGAAGATAA ACCACCAAAC ACCAAAGATC TGGATGTCTT TCTTCGGAAA CAAGAATCAG 
     3241 GGTTTGGCTT CAGGGTGCTA GGAGGAGATG GACCTGACCA GTCTATATAT ATTGGGGCCA 
     3301 TCATTCCCCT GGGAGCAGCT GAAAAAGATG GTAGGCTCCG TGCTGCTGAT GAATTGATGT 
     3361 GTATTGATGG GATTCCTGTT AAAGGAAAAT CACACAAGCA AGTATTAGAC TTAATGACTA 
     3421 CTGCTGCTCG AAATGGACAC GTGCTACTAA CTGTCAGGAG GAAGATCTTC TATGGAGAGA 
     3481 AACAACCCGA GGACGAAAGC CATCAAGCCT TCTCACAGAA CGGATCTCCT CGCCTGAATC 
     3541 GGGCAGAGCT TCCAACCAGG TCTGCCCCAC AGGAGGCCTA TGATGTCACC TTACAGAGGA 
     3601 AAGAAAATGA AGGGTTTGGT TTTGTCATCC TCACCTCTAA AAGCAAGCCG CCCCCAGGAG 
     3661 TTATTCCTCA TAAAATTGGC CGGGTCATAG ACGGAAGTCC AGCTGATCGT TGTGGAGGAC 
     3721 TGAAAGTTGG AGATCACATC TCTGCTGTGA ATGGGCAGTC CATTGTCGAC CTATCCCATG 
     3781 ATAATATTGT TCAGCTCATC AAAGATGCTG GAGTCACCGT CACGCTGACA GTGGTTGCTG 
     3841 AAGAAGAGCA TCATGGTCCA CCATCAGGAA CAAACTCAGC CAGGCAGAGC CCAGCACTAC 
     3901 AGCACAGGCC CATGGGACAA GCACAAGCCA ACCACATACC TGGGGACAGA ATTGCCCTAG 
     3961 AAGGTGAAAT TGGGAGAGAT GTCTGCAGTT CTTACAGACA TTCCTGGTCT GACCATAAGC 
     4021 ACCTTGCACA GCCTGACACT GCAGTGATTT CAGTTGTGGG CAGTCGGCAC AATCAGAGCC 
     4081 TTGGCTGTTA CCCTGTGGAG CTGGAGAGAG GCCCTCGAGG CTTTGGGTTC AGCCTCCGCG 
     4141 GAGGGAAGGA GTACAACATG GGGCTGTTCA TCCTGCGCCT GGCCGAGGAC GGGCCCGCCA 
     4201 TCAAAGACGG CAGGATTCAC GTTGGTGACC AGATCGTTGA AATCAATGGG GAACCGACAC 
     4261 AAGGCATCAC ACACACTCGA GCAATTGAGC TCATTCAGGC TGGTGGGAAT AAAGTCCTCC 
     4321 TCCTTCTGAG GCCAGGAACT GGCTTGATAC CTGACCACGG TGATTGGGAT ACTAATAGTC 
     4381 CTTCATCTTC CAATGTCATT TATGATGAAC AGCCGCCACC TCTCCCATCT TCACATTCTG 
     4441 CTTCCACATT TGAAGAGTCT CATGTGCCAG CAACTGAAGA CTCTCTGACT AGAGTTCAGA 
     4501 TATGTGAAAA GGCAGAAGAA TTAAAGGACA CTGTACAAGA AAAGAAAAGC ACTTTAAATG 
     4561 GAAGCCAGCC TGAGATGAAG TATCAGTCTG TCCACAAAAC TATGAGTAAG AAGGATCCAC 
     4621 CCAGAGGTTC CGGGCATGGC GAGAAGAGTC GACTAAAAGG AGAAAATGGT GTTACACGGA 
     4681 GAGGTAGATC TGCTAGTCCC AAAAAGTCAG TTAACCGACA TTCAGAGGAA CATTTGGAAA 
     4741 AGATTCCCAG GCCCCTAAAA AGTGATCCCA AAGAAAAATC TAGAGACCGC TCACTGAGCC 
     4801 CTAGGAAAGG AGAAAGTAAA GGTCGGCTCA CCATTAAGGC GGGCTCTGGA CAAGATCCCA 
     4861 ATAGAAAAGA CAGAGGACGG TCTTCTAGCC CCAAGAAGCA GCAAAAGATA GGAGGCAACA 
     4921 GCCTGTCAAA CACTGAAGGC AAATTATCAG AGGCTGGGAG CCGAAGAGCA GCAGGCCACC 
     4981 CTAGAGACAG CACTGAACAG CTCCCAGATG GGAGAGAAAA GTCAGGGGTC AGCAGGAAAG 
     5041 ATCTGAAGCA GAGTCAGCCA GGAAAAACCA GAACCAAGTC TCCAGAGAAA AAGAGCAGTA 
     5101 AAGTTGATGA AACATCTCTC CCATCCAAAA AGACTAGCAG CACTGCTGGT AGGGTAGTAT 
     5161 CTGAAAAGGA AAAAGGAAAG AAGCCAACAG CAGGAGAAAC AAGTAGAGAA ACAGTAGAGC 
     5221 ACACCCAGAT CTCAGCAAAG CAGCTCAAGC AGGAAGCTCA AGAGAAGACG GCATTAGGTA 
     5281 ACGCAGACGA TCACAAAGGC AGAGAATCAG AGGTCACGGA CAGATGCAGG GAGCGTGCGG 
     5341 GGTGCACACC TCAAAGTAGC TCTCTAGTCA AGAAAGCACC CATCACTCCA GGACCCTGGA 
     5401 GGGTGCCACG TGCAAATAAA GTCACAGGCA CTACTGGCAT GGCTGACAAA CAGCTGACGC 
     5461 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     5521 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     5581 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     5641 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     5701 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     5761 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     5821 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     5881 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     5941 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     6001 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     6061 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     6121 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     6181 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     6241 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     6301 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     6361 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     6421 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     6481 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     6541 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     6601 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     6661 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     6721 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     6781 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     6841 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     6901 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     6961 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     7021 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     7081 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     7141 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     7201 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     7261 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     7321 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     7381 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     7441 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     7501 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     7561 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     7621 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     7681 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     7741 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     7801 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     7861 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     7921 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     7981 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     8041 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     8101 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     8161 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     8221 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     8281 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     8341 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     8401 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     8461 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     8521 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     8581 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     8641 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     8701 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     8761 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     8821 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     8881 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     8941 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     9001 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     9061 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     9121 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     9181 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     9241 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     9301 GCGAATTTT
//