LOCUS       MR226566                9018 bp    DNA     circular  SYN 
DEFINITION  Met (Myc-DDK-tagged) - Mouse met proto-oncogene (Met)
ACCESSION   NM_008591
VERSION     NM_008591.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5175..5266 
                     /label="Myc-DDK"  
     misc_feature    5296..5881 
                     /label="PolyA signal"  
     misc_feature    5353..5372 
                     /label="Restriction Enzyme"  
     misc_feature    6157..6829 
                     /label="ColE1"  
     misc_feature    7259..8053 
                     /label="Kan/Neo"  
     misc_feature    8088..8390 
                     /label="SV40 ori"  
     misc_feature    8476..8953 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5157..5163 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5157 
                     /label="Met"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAGGCTCCC ACCGTGCTGG CACCTGGCAT TCTGGTGCTG CTGTTGTCCT 
     1081 TGGTGCAGAG GAGCCATGGG GAGTGCAAGG AGGCCCTAGT GAAGTCTGAG ATGAACGTGA 
     1141 ACATGAAGTA TCAGCTCCCC AACTTCACGG CAGAAACCCC CATCCAGAAT GTCGTCCTAC 
     1201 ACGGCCATCA TATTTATCTC GGAGCCACAA ACTACATTTA TGTTTTAAAT GACAAAGACC 
     1261 TTCAGAAGGT ATCCGAATTC AAGACCGGGC CCGTGTTGGA ACACCCAGAT TGTTTACCTT 
     1321 GTCGGGACTG CAGCAGCAAA GCCAATTCAT CAGGAGGGGT TTGGAAAGAC AACATCAACA 
     1381 TGGCTCTGCT TGTTGACACA TACTATGATG ATCAACTCAT TAGCTGTGGC AGTGTCAACA 
     1441 GAGGGACTTG CCAGCGGCAT GTCCTTCCTC CTGACAATTC TGCTGACATC CAGTCTGAGG 
     1501 TCCACTGCAT GTTCTCCCCA GAAGAGGAGT CAGGGCAGTG TCCTGACTGT GTAGTGAGTG 
     1561 CCCTCGGAGC CAAAGTCCTC CTGTCGGAAA AGGACCGGTT CATCAATTTC TTTGTGGGGA 
     1621 ATACGATCAA TTCCTCCTAT CCTCCTGGTT ATTCACTGCA TTCGATATCG GTGAGACGGC 
     1681 TGAAGGAAAC CCAAGATGGT TTTAAGTTTT TGACAGACCA GTCCTATATT GATGTCTTAC 
     1741 CAGAATTCCA AGATTCCTAC CCCATAAAGT ACATACATGC CTTCGAAAGC AACCATTTTA 
     1801 TTTACTTTCT GACTGTCCAA AAGGAAACTC TAGATGCTCA GACTTTTCAT ACAAGAATAA 
     1861 TCAGGTTCTG TTCCGTAGAC TCTGGGTTGC ACTCCTACAT GGAAATGCCC CTGGAATGCA 
     1921 TCCTGACAGA AAAAAGAAGG AAGAGATCCA CAAGGGAAGA AGTGTTTAAT ATCCTCCAAG 
     1981 CCGCGTATGT CAGTAAACCA GGGGCCAATC TTGCTAAGCA AATAGGAGCT AGCCCTTCTG 
     2041 ATGACATTCT CTTCGGGGTG TTTGCACAAA GCAAGCCAGA TTCTGCTGAA CCTGTGAATC 
     2101 GATCAGCAGT CTGTGCATTC CCCATCAAAT ATGTCAATGA CTTCTTCAAC AAGATTGTCA 
     2161 ACAAAAACAA CGTGAGATGT CTCCAGCATT TTTACGGACC CAACCATGAG CACTGTTTCA 
     2221 ATAGGACCCT GCTGAGAAAC TCTTCCGGCT GTGAAGCGCG CAGTGACGAG TATCGGACAG 
     2281 AGTTTACCAC GGCTTTGCAG CGCGTCGACT TATTCATGGG CCGGCTTAAC CAAGTGCTCC 
     2341 TGACATCCAT CTCCACCTTC ATCAAAGGTG ACCTCACCAT TGCTAATCTA GGGACGTCAG 
     2401 AAGGTCGCTT CATGCAGGTG GTGCTCTCTC GAACAGCACA CCTCACTCCT CATGTGAACT 
     2461 TCCTCCTGGA CTCCCATCCT GTATCTCCAG AAGTTATTGT TGAGCATCCA TCAAATCAAA 
     2521 ATGGCTATAC ATTGGTTGTC ACAGGAAAGA AGATCACCAA GATTCCATTG AATGGCCTGG 
     2581 GCTGTGGACA TTTCCAATCC TGCAGTCAGT GCCTCTCTGC CCCTTACTTT ATACAGTGTG 
     2641 GCTGGTGCCA CAATCAATGT GTGCGTTTTG ATGAATGCCC CAGCGGTACA TGGACTCAAG 
     2701 AGATCTGTCT GCCGGCGGTT TATAAGGTGT TCCCCACCAG CGCGCCCCTT GAAGGAGGAA 
     2761 CAGTGTTGAC CATATGTGGC TGGGACTTTG GATTCAGGAA GAATAATAAA TTTGATTTAA 
     2821 GGAAAACCAA AGTTCTGCTT GGCAACGAGA GCTGTACCTT GACCTTAAGC GAGAGCACGA 
     2881 CAAATACGTT GAAATGCACA GTTGGTCCCG CGATGAGTGA GCACTTCAAT GTGTCTGTAA 
     2941 TTATCTCAAA CAGTCGAGAG ACGACGCAAT ACAGTGCATT CTCCTATGTA GATCCTGTAA 
     3001 TAACAAGCAT TTCTCCGAGG TACGGCCCTC AGGCTGGAGG CACCTTACTC ACTCTTACTG 
     3061 GGAAATACCT CAACAGTGGC AATTCTAGAC ACATTTCAAT TGGAGGGAAA ACATGTACTT 
     3121 TAAAAAGTGT ATCAGATAGT ATTCTTGAAT GCTACACCCC AGCCCAAACT ACCTCTGATG 
     3181 AGTTTCCTGT GAAATTGAAG ATTGACTTGG CTAACCGAGA GACCAGCAGC TTCAGTTACC 
     3241 GGGAAGACCC CGTTGTCTAT GAAATCCACC CGACCAAATC TTTTATTAGT GGTGGAAGCA 
     3301 CAATAACGGG TATTGGGAAG ACCCTGAACT CGGTTAGCCT CCCAAAGCTG GTAATAGATG 
     3361 TGCATGAAGT GGGTGTGAAC TACACAGTGG CATGTCAGCA TCGCTCAAAT TCAGAGATCA 
     3421 TCTGCTGCAC TACTCCTTCA CTGAAACAGC TGGGCCTGCA ACTCCCCCTG AAGACCAAAG 
     3481 CCTTCTTCCT GTTAGACGGG ATTCTTTCCA AACACTTTGA TCTCACTTAT GTGCATAATC 
     3541 CTGTGTTTGA GCCTTTTGAA AAGCCAGTAA TGATCTCAAT AGGCAATGAA AATGTAGTGG 
     3601 AAATTAAGGG AAACAATATT GACCCTGAAG CAGTTAAAGG TGAAGTGTTA AAAGTTGGAA 
     3661 ATCAGAGCTG CGAGAGTCTC CACTGGCACT CTGGAGCTGT GTTGTGTACA GTCCCCAGTG 
     3721 ACCTGCTCAA ACTGAACAGC GAGCTAAATA TAGAGTGGAA GCAAGCAGTC TCTTCAACTG 
     3781 TTCTTGGAAA AGTGATCGTT CAACCGGATC AGAATTTTGC AGGATTGATC ATTGGTGCGG 
     3841 TCTCAATATC AGTAGTAGTT TTGTTATTAT CCGGGCTCTT CCTGTGGATG AGAAAGAGAA 
     3901 AGCATAAAGA TCTGGGCAGT GAATTAGTTC GCTATGACGC AAGAGTACAC ACTCCTCATT 
     3961 TGGATAGGCT TGTAAGTGCC CGAAGTGTAA GTCCAACTAC AGAGATGGTT TCAAATGAGT 
     4021 CTGTAGACTA CAGAGCTACT TTTCCAGAAG ACCAGTTTCC CAACTCCTCT CAGAATGGAG 
     4081 CATGCAGACA AGTGCAATAC CCTCTGACAG ACCTGTCCCC TATCCTGACA AGTGGAGACT 
     4141 CTGATATATC CAGCCCATTA CTACAAAATA CTGTTCACAT TGACCTCAGT GCTCTAAATC 
     4201 CAGAGCTGGT CCAAGCAGTT CAGCACGTAG TGATTGGACC CAGCAGCCTG ATTGTGCATT 
     4261 TCAATGAAGT CATAGGAAGA GGGCATTTTG GCTGTGTCTA TCATGGGACT TTGCTGGACA 
     4321 ATGACGGAAA GAAAATTCAC TGTGCTGTGA AATCCTTAAA TAGAATCACA GATATAGAAG 
     4381 AGGTCTCCCA GTTTCTGACT GAGGGAATCA TCATGAAAGA CTTCAGCCAT CCCAATGTTC 
     4441 TCTCACTCTT GGGAATCTGC CTGAGGAGTG AAGGGTCTCC TCTGGTGGTC CTGCCCTATA 
     4501 TGAAGCATGG AGATCTGCGA AATTTCATTC GAAACGAGAC TCATAATCCA ACTGTGAAAG 
     4561 ATCTTATAGG ATTTGGCCTT CAAGTAGCCA AAGGCATGAA ATATCTTGCC AGCAAAAAGT 
     4621 TTGTCCACAG AGACTTAGCT GCAAGAAACT GCATGTTGGA TGAAAAATTC ACTGTCAAGG 
     4681 TTGCTGATTT CGGTCTTGCC AGAGACATGT ACGATAAAGA GTACTATAGT GTCCACAACA 
     4741 AGACGGGTGC CAAGCTACCA GTAAAGTGGA TGGCTTTAGA GAGTCTGCAA ACGCAGAAGT 
     4801 TCACCACCAA GTCAGATGTG TGGTCCTTTG GTGTGCTCCT CTGGGAGCTC ATGACGAGAG 
     4861 GAGCCCCTCC TTATCCCGAC GTGAACACAT TTGATATCAC TATCTACCTG TTGCAAGGCA 
     4921 GAAGACTCTT GCAACCAGAA TACTGTCCAG ACGCCTTGTA CGAAGTGATG CTAAAATGCT 
     4981 GGCACCCCAA AGCGGAAATG CGCCCGTCCT TTTCCGAACT GGTCTCCAGG ATATCCTCAA 
     5041 TCTTCTCCAC GTTCATTGGG GAACACTACG TCCACGTGAA CGCTACTTAT GTGAATGTAA 
     5101 AATGTGTTGC TCCATATCCT TCTCTGTTGC CATCCCAAGA CAACATTGAT GGCGAGGGGA 
     5161 ACACAACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     5221 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     5281 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     5341 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     5401 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     5461 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     5521 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     5581 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     5641 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     5701 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     5761 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     5821 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     5881 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     5941 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     6001 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     6061 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     6121 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     6181 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     6241 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     6301 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     6361 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     6421 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     6481 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     6541 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     6601 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     6661 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     6721 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     6781 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     6841 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     6901 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     6961 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     7021 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     7081 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     7141 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     7201 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     7261 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     7321 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     7381 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     7441 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     7501 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     7561 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     7621 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     7681 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     7741 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     7801 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     7861 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     7921 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     7981 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     8041 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     8101 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     8161 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     8221 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     8281 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     8341 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     8401 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     8461 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     8521 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     8581 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     8641 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     8701 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     8761 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     8821 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     8881 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     8941 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     9001 AAATTTAACG CGAATTTT
//