LOCUS       MR226542                9411 bp    DNA     circular  SYN 
DEFINITION  Nrxn1 (Myc-DDK-tagged) - Mouse neurexin I (Nrxn1), transcript variant 1
ACCESSION   NM_020252
VERSION     NM_020252.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5566..5657 
                     /label="Myc-DDK"  
     misc_feature    5687..6272 
                     /label="PolyA signal"  
     misc_feature    5744..5763 
                     /label="Restriction Enzyme"  
     misc_feature    6548..7220 
                     /label="ColE1"  
     misc_feature    7650..8444 
                     /label="Kan/Neo"  
     misc_feature    8479..8781 
                     /label="SV40 ori"  
     misc_feature    8867..9344 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5541..5548 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5541 
                     /label="Nrxn1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGGACGGCG CTGGTCCAGC GCGGGGGCTG CTGTCTCCTC TGCCTGTCGC 
     1081 TGCTGCTGCT GGGCTGCTGG GCAGAGCTGG GCAGCGGGCT GGAGTTCCCG GGCGCCGAGG 
     1141 GCCAGTGGAC GCGCTTCCCC AAGTGGAACG CGTGCTGCGA GAGCGAGATG AGCTTCCAGC 
     1201 TGAAGACGCG CAGTGCCCGC GGCCTCGTGC TCTACTTCGA CGACGAGGGC TTCTGCGACT 
     1261 TCCTCGAGCT CATCCTGACG CGCGGCGGCC GCCTGCAGCT CAGCTTCTCC ATCTTCTGCG 
     1321 CCGAGCCCGC CACGCTGTTG GCCGACACGC CGGTCAACGA CGGCGCCTGG CACAGCGTGC 
     1381 GCATCCGCCG CCAGTTCCGC AACACCACGC TCTACATCGA CCGCGCCGAG GCCAAGTGGG 
     1441 TGGAGGTCAA GTCCAAGCGC AGGGACATGA CGGTGTTCAG CGGGCTCTTC GTGGGCGGCC 
     1501 TGCCACCCGA ACTGCGCGCC GCGGCCCTCA AGCTCACGCT GGCCTCGGTG CGCGAGCGCG 
     1561 AGCCCTTCAA GGGCTGGATC CGTGACGTCC GGGTCAACTC GTCACAGGCC CTGCCGGTGG 
     1621 ACGGCGGCGA GGTCAAGCTG GACGACGAGC CTCCCAACAG CGGTGGCGGG AGCCCATGCG 
     1681 AGGCGGGCGA AGAAGGCGAG GGCGGCGTGT GCCTCAACGG GGGCGTGTGC TCCGTGGTCG 
     1741 ACGACCAGGC CGTATGCGAC TGCTCGAGGA CCGGCTTCCG TGGCAAGGAC TGCAGCCAAG 
     1801 AAGACAACAA TGTGGAAGGT CTGGCACACC TGATGATGGG CGACCAAGGT AAAAGTAAAG 
     1861 GAAAAGAAGA GTATATTGCC ACGTTCAAGG GATCTGAATA CTTCTGCTAC GACTTATCTC 
     1921 AAAACCCCAT TCAAAGCAGC AGTGATGAAA TAACTCTGTC CTTTAAAACT CTTCAAAGGA 
     1981 ACGGACTGAT GCTTCACACA GGGAAATCAG CTGATTATGT CAATCTTGCA TTGAAAAATG 
     2041 GAGCTGTCTC TCTGGTCATT AATTTGGGAT CAGGGGCCTT TGAAGCACTA GTGGAGCCTG 
     2101 TGAATGGAAA GTTTAATGAT AATGCCTGGC ATGATGTGAA AGTCACCAGG AATCTGCGTC 
     2161 AGCACTCAGG CATTGGACAC GCTATGGTGA CAATATCAGT GGATGGAATT CTTACCACAA 
     2221 CGGGCTACAC GCAAGAAGAT TACACCATGC TGGGGTCTGA TGACTTTTTC TATGTTGGAG 
     2281 GCAGCCCCAG CACAGCCGAC CTTCCAGGGT CACCAGTCAG TAACAACTTT ATGGGCTGTC 
     2341 TCAAAGAGGT TGTATATAAA AATAATGATG TAAGGCTGGA ATTATCGCGA CTTGCCAAGC 
     2401 AAGGAGATCC TAAGATGAAG ATTCACGGTG TGGTGGCTTT TAAGTGTGAA AATGTGGCAA 
     2461 CCTTAGACCC AATAACTTTT GAGACCCCGG AATCTTTCAT TTCTTTGCCT AAGTGGAATG 
     2521 CAAAGAAAAC AGGCTCAATA TCATTTGATT TCCGTACAAC GGAGCCAAAT GGCCTCATCC 
     2581 TGTTTAGCCA TGGCAAGCCA AGACATCAGA AGGATGCCAA GCACCCACAG ATGATTAAGG 
     2641 TTGACTTTTT TGCTATTGAG ATGCTGGATG GCCACTTGTA CCTCCTCTTA GACATGGGAT 
     2701 CAGGTACTAT AAAAATCAAA GCCCTGCAGA AGAAAGTCAA TGATGGAGAA TGGTATCACG 
     2761 TGGATTTCCA ACGGGATGGA CGGTCAGGTA CCATTTCTGT CAACACACTA CGCACTCCCT 
     2821 ACACAGCTCC CGGTGAGAGT GAGATCCTGG ACCTAGATGA TGAGTTGTAC CTGGGTGGCT 
     2881 TGCCAGAAAA TAAGGCTGGC CTAGTCTTCC CTACCGAGGT GTGGACTGCT CTGCTCAACT 
     2941 ACGGCTACGT GGGCTGCATC CGGGACCTAT TTATTGATGG CCAGAGCAAA GACATCCGGC 
     3001 AGATGGCGGA AATTCAGAGC ACTGCTGGAG TGAAGCCATC CTGCTCAAAA GAGACAGCAA 
     3061 AACCGTGCCT TAGCAATCCT TGCAAAAACA ATGGCATGTG CAGAGATGGC TGGAACAGAT 
     3121 ACGTCTGCGA TTGCTCCGGA ACAGGCTATC TCGGCAGGTC CTGTGAGAGA GAGGCAACGG 
     3181 TTTTGAGCTA CGACGGGAGC ATGTTCATGA AAATCCAGCT TCCGGTGGTG ATGCACACTG 
     3241 AGGCTGAGGA CGTGTCCTTG CGGTTCCGAT CCCAGCGTGC ATATGGTATC CTGATGGCGA 
     3301 CCACTTCTAG AGACTCTGCA GACACCCTTC GATTGGAGCT AGATGCAGGA CGTGTGAAAC 
     3361 TCACGGTCAA TCTAGATTGT ATCAGGATTA ACTGTAATTC CAGCAAAGGT CCTGAGACCC 
     3421 TTTTTGCTGG CTATAACCTC AATGATAATG AATGGCACAC AGTGCGTGTG GTTCGGCGAG 
     3481 GAAAAAGTTT AAAGCTAACC GTGGATGATC AACAAGCCAT GACAGGCCAA ATGGCAGGTG 
     3541 ATCATACAAG GCTGGAGTTT CATAACATAG AGACAGGCAT CATCACCGAA CGACGCTACC 
     3601 TTTCTTCTGT CCCCTCCAAT TTCATTGGGC ACCTGCAAAG CCTGACATTT AATGGAATGG 
     3661 CATACATAGA CTTGTGCAAA AATGGTGATA TAGATTACTG TGAACTCAAT GCCAGGTTTG 
     3721 GATTCAGGAA CATCATCGCA GATCCTGTCA CCTTCAAGAC CAAATCGAGC TATGTTGCCT 
     3781 TAGCTACATT GCAAGCCTAC ACCTCTATGC ATCTATTTTT CCAATTCAAG ACAACATCTC 
     3841 TAGATGGACT AATTCTGTAT AACAGTGGGG ATGGAAATGA CTTTATTGTG GTTGAATTAG 
     3901 TTAAAGGGTA CTTACATTAT GTGTTTGACT TGGGAAACGG TGCTAACCTC ATCAAAGGGA 
     3961 GCTCAAACAA ACCACTCAAC GACAATCAGT GGCACAATGT GATGATTTCA CGGGACACCA 
     4021 GCAATCTCCA CACAGTAAAG ATCGACACAA AAATCACAAC ACAAATCACT GCGGGAGCCA 
     4081 GAAATCTAGA CCTCAAGAGT GACTTGTATA TTGGAGGAGT GGCTAAAGAA ACATATAAAT 
     4141 CCCTGCCAAA ACTGGTCCAT GCCAAAGAAG GCTTTCAAGG TTGCCTGGCA TCTGTGGACT 
     4201 TGAATGGCAG ACTTCCAGAT CTCATCTCGG ATGCTCTCTT CTGCAATGGG CAAATTGAGA 
     4261 GAGGATGCGA AGGGCCCAGC ACAACCTGCC AAGAGGATTC ATGTTCAAAT CAAGGCGTTT 
     4321 GCTTGCAGCA ATGGGATGGC TTCAGCTGTG ACTGTAGCAT GACTTCCTTC AGTGGCCCAC 
     4381 TCTGCAATGA CCCTGGGACA ACATATATCT TTAGCAAAGG TGGTGGACAG ATTACATATA 
     4441 AGTGGCCTCC CAATGACCGC CCCAGTACAC GAGCAGACAG GCTGGCCATC GGATTTAGCA 
     4501 CTGTCCAGAA GGAAGCCGTG TTGGTGCGTG TGGACAGTTC CTCAGGACTG GGTGACTACC 
     4561 TGGAGCTGCA CATACACCAA GGAAAAATTG GAGTTAAGTT TAATGTTGGG ACAGATGACA 
     4621 TCGCCATCGA AGAGTCTAAT GCAATCATTA ATGATGGGAA ATACCATGTA GTACGTTTCA 
     4681 CAAGGAGTGG TGGCAATGCC ACGTTACAGG TGGACAGCTG GCCAGTTATC GAACGCTACC 
     4741 CTGCAGGAAA CAATGATAAC GAGCGCCTGG CGATTGCTAG ACAGCGAATT CCATATCGAC 
     4801 TTGGTCGAGT AGTTGATGAA TGGCTACTCG ACAAAGGGCG TCAGCTCACA ATCTTCAATA 
     4861 GCCAAGCAAC CATAATAATT GGCGGGAAAG AGCAGGGCCA GCCCTTCCAG GGCCAGCTCT 
     4921 CTGGTCTTTA CTACAATGGC TTGAAAGTTC TGAATATGGC AGCAGAGAAC GATGCCAACA 
     4981 TCGCCATAGT GGGGAATGTG AGGCTGGTCG GTGAAGTGCC TTCCTCTATG ACAACTGAGT 
     5041 CGACAGCCAC TGCCATGCAG TCTGAGATGT CCACCTCAAT CATGGAGACC ACCACAACCC 
     5101 TGGCTACCAG CACAGCTAGA AGAGGCAAGC CCCCCACAAA GGAACCCATC AGCCAGACCA 
     5161 CAGATGACAT CCTTGTGGCC TCGGCAGAGT GTCCCAGTGA CGATGAGGAC ATTGACCCCT 
     5221 GTGAGCCGAG CTCAGGTGGG TTAGCCAACC CCACCAGAGT AGGTGGCCGT GAACCATACC 
     5281 CAGGCTCTGC AGAGGTGATT CGGGAGTCCA GCAGTACCAC TGGCATGGTG GTGGGGATTG 
     5341 TCGCAGCAGC CGCTCTGTGC ATCCTCATCC TCCTCTATGC CATGTACAAG TACAGGAACC 
     5401 GGGATGAAGG GTCGTACCAC GTGGATGAGA GTCGAAACTA CATCAGTAAC TCAGCACAGT 
     5461 CCAATGGGGC TGTGGTCAAG GAGAAGCAAC CCAGCAGTGC TAAAAGCGCC AACAAAAACA 
     5521 AGAAAAACAA GGATAAGGAG TATTACGTCA GCGGACCGAC GCGTACGCGG CCGCTCGAGC 
     5581 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     5641 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     5701 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     5761 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     5821 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     5881 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     5941 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     6001 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     6061 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     6121 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     6181 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     6241 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     6301 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     6361 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     6421 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     6481 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     6541 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     6601 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     6661 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     6721 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     6781 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     6841 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     6901 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     6961 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     7021 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     7081 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     7141 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     7201 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     7261 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     7321 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     7381 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     7441 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     7501 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     7561 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     7621 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     7681 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     7741 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     7801 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     7861 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     7921 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     7981 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     8041 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     8101 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     8161 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     8221 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     8281 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     8341 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     8401 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     8461 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     8521 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     8581 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     8641 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     8701 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     8761 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     8821 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     8881 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     8941 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     9001 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     9061 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     9121 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     9181 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     9241 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     9301 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     9361 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
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