LOCUS       MR226531                9186 bp    DNA     circular  SYN 
DEFINITION  Ptprt (Myc-DDK-tagged) - Mouse protein tyrosine phosphatase, receptor type, T (Ptprt)
ACCESSION   NM_021464
VERSION     NM_021464.5
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5343..5434 
                     /label="Myc-DDK"  
     misc_feature    5464..6049 
                     /label="PolyA signal"  
     misc_feature    5521..5540 
                     /label="Restriction Enzyme"  
     misc_feature    6325..6997 
                     /label="ColE1"  
     misc_feature    7427..8221 
                     /label="Kan/Neo"  
     misc_feature    8256..8558 
                     /label="SV40 ori"  
     misc_feature    8644..9121 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5325..5331 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5325 
                     /label="Ptprt"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGGAGCCTT GGCGGGCTCG CCCTCTGCCT GCTCCGGCTC CTGCTCCTGG 
     1081 GGCTGCAGCG GCCGCCGTTG CCCGGCGCCG GAGCGCAGAG CGCCGCAGGT GGCTGTTCTT 
     1141 TTGACGAACA TTACAGCAAC TGCGGGTATA GCGTGGCTCT GGGAACCAAT GGGTTTACCT 
     1201 GGGAGCAGAT TAACACATGG GAGAAGCCAA TGCTGGACCC AGCTGTGCCC ACAGGGTCCT 
     1261 TCATGATGGT GAACAGCTCT GGAAGGGCTT CAGGCCAGAA AGCCCATCTT CTTCTGCCAA 
     1321 CCTTGAAGGA GAATGACACT CACTGCATTG ACTTCCATTA CTACTTCTCC AGTCGAGATC 
     1381 GCTCCAGCCC GGGAGCCTTG AATGTCTACG TGAAGGTGAA TGGTGGACCC CAAGGGAACC 
     1441 CTGTCTGGAA CGTATCTGGC GTCGTCACTG AGGGCTGGGT GAAGGCAGAG CTTGCCATCA 
     1501 GCACCTTCTG GCCTCATTTC TATCAGGTGA TATTTGAATC CGTCTCTTTG AAAGGTCATC 
     1561 CTGGTTACAT CGCTGTGGAC GAAGTTCGGG TCCTTGCTCA TCCATGCAGA AAAGCACCTC 
     1621 ATTTCCTGCG ACTCCAAAAC GTTGAGGTGA ATGTGGGGCA GAACGCCACG TTTCAGTGCA 
     1681 TTGCTGGTGG AAAGTGGTCC CAGCATGACA AACTTTGGCT CCAGCAATGG AATGGCAGAG 
     1741 ACACAGCCCT CATGGTCACC CGGGTGGTCA ACCACAGACG CTTCTCAGCC ACAGTGAGTG 
     1801 TGGCAGACAC CTCTCAACGC AGTATCTCCA AGTATCGCTG CGTGATCCGC TCAGATGGTG 
     1861 GGTCTGGTGT GTCCAACTAT GCAGAGCTGA TTGTGAAAGA GCCTCCCACG CCCATTGCTC 
     1921 CCCCGGAACT ACTGGCCGTG GGTGCCACCT ACCTGTGGAT TAAACCAAAT GCCAACTCCA 
     1981 TTATTGGGGA TGGCCCCATC ATACTGAAGG AGGTAGAATA CCGCACAACC ACAGGAACCT 
     2041 GGGCCGAGAC CCACATCGTG GACTCTCCCA ACTACAAGCT CTGGCATCTG GACCCTGATG 
     2101 TGGAGTATGA GATCCGGGTG CTGCTCACAC GACCAGGAGA GGGGGGCACA GGACCACCAG 
     2161 GACCACCCCT AACTACCAGG ACCAAGTGTG CCGATCCCGT GCATGGCCCG CAGAATGTGG 
     2221 AGATTGTGGA CATTCGGGCT CGGCAGCTGA CCCTGCAGTG GGAACCCTTT GGCTATGCAG 
     2281 TGACCCGCTG CCACAGCTAC AACCTCACAG TGCAGTACCA GTACGTGTTC AACCAGCAGC 
     2341 AGTATGAAGC TGAAGAGGTG ATCCAGACAT CTTCCCACTA TACCCTTCGG GGTCTACGGC 
     2401 CCTTCATGAC CATCAGACTG CGGCTGCTAC TGTCCAACCC TGAGGGCCGG ATGGAGAGTG 
     2461 AGGAGTTGGT GGTACAGACA GAAGAGGATG TTCCAGGAGC TGTTCCTCTC GAGTCCATCC 
     2521 AAGGGGGTCC CTTTGAGGAG AAGATCTACA TCCAGTGGAA ACCTCCCAAT GAGACCAATG 
     2581 GGGTCATCAC ACTCTATGAG ATTAACTACA AGGCTGTGGG CTCACTGGAT CCAAGTGCTG 
     2641 ACCTCTCCAG CCAGAGGGGA AAGGTGTTCA AACTCCGGAA TGAAACCCAC CACCTCTTTG 
     2701 TGGGTCTGTA CCCTGGGACT ACCTACTCTT TCACCATCAA GGCCAGCACA GCCAAGGGCT 
     2761 TTGGACCCCC AGTCACTACT CGGATTGCCA CCAAGATTTC AGCTCCATCA ATGCCTGAAT 
     2821 ATGACGCAGA TACCCCACTC AATGAAACAG ATACAACCAT CACGGTGATG CTCAAACCCG 
     2881 CCCAGTCCAG GGGAGCCCCA GTCAGTGTTT ACCAGCTGGT TGTCAAGGAG GAGCGACTCC 
     2941 AGAAGTCTCG GAGAGCAGCT GACATCATTG AGTGTTTCTC AGTACCTGTG AGCTACCGGA 
     3001 ACGCCTCCAA CCTCGATTCG CTACACTACT TTGCTGCTGA GCTGAAGCCC TCCAACCTGC 
     3061 CTGTCACCCA GCCATTTACA GTGGGAGACA ACAAGACCTA CAATGGCTAC TGGAACCCTC 
     3121 CTCTCTCCCC ATTGAAGAGC TACAGCATCT ACTTCCAGGC CCTCAGCAAA GCAAATGGAG 
     3181 AGACAAAAAT CAACTGTGTG CGTCTGGCTA CAAAAGGTGC TTCCACTCAG AATTCTAACA 
     3241 CTGTGGAGCC GGAGAAGCAG GTCGACAACA CGGTGAAGAT GGCTGGCGTG ATTGCTGGCC 
     3301 TCCTCATGTT CATCATCATT CTCCTGGGGG TGATGCTGAC CATCAAAAGG AGGAAGCTGG 
     3361 CTAAGAAGCA GAAGGAGACC CAGAGTGGAG CCCAGAGGGA GATGGGTCCT GTGGCCTCGA 
     3421 CTGACAAGCC TACCGCCAAG CTCGGCACCA ACCGCAATGA TGAAGGCTTC TCCTCCAGCT 
     3481 CTCAGGATGT TAATGGATTC ACAGATGGCA GCCGTGGGGA GCTGTCTCAG CCTACCCTCA 
     3541 CCATTCAGAC TCATCCCTAC CGGACTTGTG ACCCTGTAGA GATGAGCTAT CCCCGGGACC 
     3601 AGTTCCAGCC TGCCATCCGG GTGGCGGACC TGCTTCAACA CATCACCCAG ATGAAGAGAG 
     3661 GCCAGGGCTA CGGGTTCAAG GAGGAATACG AGGCCTTACC AGAAGGACAG ACAGCTTCGT 
     3721 GGGACACAGC CAAGGAAGAT GAAAACCGCA ATAAGAATCG ATACGGAAAC ATCATATCTT 
     3781 ATGACCACTC TCGAGTAAGG CTGTTGGTGC TGGATGGAGA CCCTCACTCA GACTACATCA 
     3841 ATGCCAACTA CATTGACGGG TACCACCGAC CCCGGCACTA CATTGCAACC CAAGGTCCAA 
     3901 TGCAAGAGAC GGTGAAGGAC TTTTGGAGAA TGATCTGGCA GGAAAACTCT GCCAGCATCG 
     3961 TCATGGTCAC AAACCTTGTG GAAGTGGGCA GGGTGAAGTG TGTCCGATAC TGGCCAGATG 
     4021 ACACAGAGGT CTATGGAGAC ATTAAAGTCA CCCTAATAGA AACAGAGCCC CTGGCAGAAT 
     4081 ACGTCATCCG CACCTTCACA GTCCAGAAGA AAGGCTACCA TGAGATCCGG GAGCTCCGCC 
     4141 TCTTCCACTT CACCAGCTGG CCTGACCACG GTGTTCCCTG CTATGCCACT GGCCTTCTGG 
     4201 GCTTTGTCCG CCAGGTCAAG TTTCTCAATC CCCCAGAAGC TGGGCCCATA GTGGTCCATT 
     4261 GCAGTGCTGG AGCCGGGAGG ACTGGCTGCT TCATTGCGAT TGACACCATG CTCGACATGG 
     4321 CTGAGAATGA AGGGGTTGTG GACATCTTCA ACTGTGTGCG TGAGCTCCGG GCACAGAGGG 
     4381 TCAACCTGGT GCAGACAGAG GAGCAGTACG TGTTTGTGCA CGATGCCATC CTGGAAGCAT 
     4441 GCCTCTGCGG CAATACTGCC ATCCCAGTGT GTGAGTTCCG CTCTCTCTAC TACAACATCA 
     4501 GCAGGCTGGA CCCGCAGACC AACTCCAGCC AGATCAAAGA CGAGTTTCAG ACACTCAACA 
     4561 TTGTGACACC TCGAGTGCGG CCTGAAGACT GCAGCATTGG GCTCTTACCC CGGAACCATG 
     4621 ATAAGAATCG GAGCATGGAT GTCCTGCCTC TGGACCGCTG TCTACCCTTC CTCATCTCAG 
     4681 TAGATGGAGA GTCCAGCAAC TACATCAATG CAGCACTGAT GGATAGCCAC AAGCAGCCTG 
     4741 CCGCCTTCGT GGTCACCCAG CATCCTCTAC CCAACACGGT GGCAGACTTC TGGAGGCTGG 
     4801 TGTTTGATTA TAATTGTTCA TCTGTGGTGA TGCTGAACGA GATGGATACT GCTCAGCTCT 
     4861 GTATGCAGTA CTGGCCTGAG AAGACCTCCG GGTGTTATGG TCCCATCCAG GTGGAGTTTG 
     4921 TCTCTGCAGA CATCGATGAG GACATCATCC ACAGAATCTT CCGGATCTGT AACATGGCTC 
     4981 GGCCACAGGA TGGTTATCGT ATTGTCCAGC ACCTCCAGTA CATCGGCTGG CCTGCATACC 
     5041 GGGACACGCC CCCCTCTAAG CGCTCTCTGC TCAAAGTGGT CCGACGGCTG GAGAAATGGC 
     5101 AGGAGCAATA CGACGGAAGA GAGGGGCGCA CTGTGGTCCA CTGCCTAAAT GGGGGAGGCC 
     5161 GCAGTGGAAC CTTCTGTGCT ATCTGCAGTG TGTGTGAGAT GATCCAGCAG CAGAACATTA 
     5221 TTGACGTGTT CCACATTGTG AAAACCCTCC GCAACAACAA GTCCAACATG GTGGAGACGC 
     5281 TGGAACAGTA TAAATTTGTA TATGAGGTGG CACTGGAATA TTTAAGCTCC TTTACGCGTA 
     5341 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     5401 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     5461 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     5521 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     5581 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     5641 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     5701 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     5761 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     5821 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     5881 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     5941 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     6001 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     6061 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     6121 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     6181 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     6241 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     6301 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     6361 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     6421 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     6481 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     6541 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     6601 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     6661 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     6721 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     6781 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     6841 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     6901 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     6961 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     7021 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     7081 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     7141 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     7201 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     7261 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     7321 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     7381 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     7441 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     7501 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     7561 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     7621 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     7681 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     7741 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     7801 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     7861 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     7921 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     7981 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     8041 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     8101 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     8161 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     8221 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     8281 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     8341 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     8401 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     8461 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     8521 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     8581 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     8641 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     8701 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     8761 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     8821 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     8881 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     8941 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     9001 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     9061 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     9121 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     9181 AATTTT
//