LOCUS       MR226522                8703 bp    DNA     circular  SYN 
DEFINITION  Enam (Myc-DDK-tagged) - Mouse enamelin (Enam)
ACCESSION   NM_017468
VERSION     NM_017468.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4860..4951 
                     /label="Myc-DDK"  
     misc_feature    4981..5566 
                     /label="PolyA signal"  
     misc_feature    5038..5057 
                     /label="Restriction Enzyme"  
     misc_feature    5842..6514 
                     /label="ColE1"  
     misc_feature    6944..7738 
                     /label="Kan/Neo"  
     misc_feature    7773..8075 
                     /label="SV40 ori"  
     misc_feature    8161..8638 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4842..4848 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4842 
                     /label="Enam"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTTGCTGCTT CAGTGCAGAA ATCCGACTTC TCCTCCAAAG CCATGTGGCC 
     1081 TGGTACCAAA TGTAAAGATG AGTCTCCTTG TTTTCCTGGG TCTGCTTGGT GTCTCTGCTG 
     1141 CCATGCCATT CCAGATGCCA ATGCCCCGAA TGCCTGGATT TAGCAGTAAA AGTGAAGAGA 
     1201 TGATGCGATA TAATCAATTC AACTTCATGA ATGCCCCACC AATGATGCCT ATGGGCCCAT 
     1261 ATGGAAATGG TATGCCAATG CCGCCACACA TGCCTCCACA GTACCCTCCA TACCAGATGC 
     1321 CCATGTGGCC TCCACCAGTA CCCAATGGAT GGCAGCAACC CCCAATGCCC AATTTCCCAA 
     1381 GCAAGACTGA TCAAACCCAG GAGACCGCCA AACCCAACCA GACCAATCCA CAAGAGCCAC 
     1441 AGCCACAAAA GCAGCCTTTA AAGGAACCAC CAAATGAAGC AGCACGGGCC AAAGATGACG 
     1501 CCCAGCCACC TCAGCCATTC CCACCATTTG GCAATGGACT TTACCCCTAT CCACAACCAC 
     1561 CATGGCCAAT TCCACAGAGG GGACCACCAA CAGCGTTTGG ACGGCCAAAG TTCAGCAATG 
     1621 AAGAAGGAAA TCCTTACTAT GCATTTTTTG GATATCACGG CTTTGGGGGT CGTCCTTATT 
     1681 ACTCAGAAGA GATGTTTGAA GATTATGAAA AACCCAAAGA AAAAGACCCT CCTAAACCAG 
     1741 AGGACCCACC TCCAGATGAC CCACCCCCAG AGGCCTCTAC AAACTCAACT GTGCCTGATG 
     1801 CTAATGCCAC TCAATCAATT CCTGAAGGCG GAAATGACAC TAGCCCAATA GGAAACACAG 
     1861 GCCCTGGGCC GAACGCTGGG AACAATCCTA CAGTTCAAAA CGGTGTCTTC CCTCCCCCTA 
     1921 AAGTTAATGT TTCAGGCCAG GGAGTACCAA AAAGCCAAAT TCCGTGGAGA CCAAGTCAGC 
     1981 CAAATATTTA TGAGAATTAT CCTTACCCAA ATTATCCTTC AGAAAGACAA TGGCAAACCA 
     2041 CTGGTACCCA GGGGCCTAGA CAGAATGGAC CTGGCTACCG AAATCCACAA GTTGAAAGGG 
     2101 GTCCTCAGTG GAATTCCTTT GCTTGGGAAG GCAAACAAGC TACTCGTCCA GGAAACCCAA 
     2161 CTTACGGTAA ACCTCCCTCT CCTACCTCCG GGGTTAATTA TGCAGGAAAT CCAGTCCATT 
     2221 TCGGAAGAAA CCTGCCAGGG CCAAATAAAC CCTTTGTGGG AGCCAATCCG GCCTCAAATA 
     2281 AACCCTTTGT GGGAGCCAAT CCGGCCTCAA ACAAACCCTT TGTGGGAGCT AATCCGGCCT 
     2341 CAAACAAACC CTTTGTGGGA GCCAATCCGG CCTCAAATAA ACCCTTTGTG GGAGCCAATC 
     2401 CGGCCTCAAA TAAACCCTAT GTGGGAGCCA ATCCGGCCTC AAACAAACCC TTTATAGGAG 
     2461 CCAATCCGGC TGCAAACAAA CCATCTATAG GAACCAATCC AGCCGCAAAC AAACCATCTA 
     2521 TAGGAACCAA TCCAGCTGCA AATAAACCCT TTGTGAGAAA CAATGTAGGT GCAAATAAAC 
     2581 CCTTTGTGGG AACCAATCCC TCCTCAAACC AACCATTTCT GAGAAGCAAT CAGGCCTCAA 
     2641 ATAAACCATT TATGAGAAGC AATCAGGCCT CAAATAAACC ATTTGTGGGC ACCAATGTGG 
     2701 CCTCAGTGGG TCCTAAACAG GTCACTGTTA GCCACAATAT GAAAACTCAA AATCCAAAAG 
     2761 AAAAGTCACT AGGTCAAAAA GAAAGAACAG TCACTCCTAC CAAAGATGCA AGCAACCCCT 
     2821 GGAGAAGTGC TAAACAATAT GGAATTAACA ATCCAAACTA TAATTTGCCT CGCTCTGAGG 
     2881 GCAGCATGGT AGGCCCAAAT TTTAATTCCT TTGATCAACA AGAAAACTCC TACTTCTCAA 
     2941 AAGGAGCTTC CAAAAGAGTA CCAAGTCCTA ATATACAAAT CCAAAGCCAG AATTTGCCCA 
     3001 AAGGAATTGC TTTAGAGCCA AGAAGAACCC CATTTCAATC AGAAACTAAA AAACCTGAGT 
     3061 TAAAACATGG TACACACCAG CCTGCATACC CTAAGAAAAT CCCTTCTCCT ACAAGAAAAC 
     3121 ATTTCCCTGC TGAAAGAAAT ACCTGGAATC GTCAAAAAAT CCTTCCACCC TTAAAGGAAG 
     3181 ACTATGGGAG GCAAGACGAA AATTTACGTC ATCCGTCCTA TGGCTCTAGA GGAAATATTT 
     3241 TTTACCATGA ATATACCAAT CCTTATCATA ATGAAAAATC ACAGTACATT AAAAGCAATC 
     3301 CATGGGATAA GAGCTCTCCC AGTACTATGA TGCGGCCAGA AAACCCACAG TACACCATGA 
     3361 CTTCTCTAGA CCAGAAGGAG ACAGAGCAGT ACAATGAAGA GGATCCAATT GATCCAAATG 
     3421 AAGATGAATC TTTTCCAGGA CAAAGTAGAT GGGGGGATGA AGAGATGAAC TTCAAAGGAA 
     3481 ACCCAACAGT TAGGCAGTAT GAAGGTGAGC ACTACGCCTC AACCCTAGCG AAGGAATACC 
     3541 TTCCTTATTC CTTAAGTAAT CCACCAAAAC CCAGTGAAGA TTTTCCTTAC AGTGAATTCT 
     3601 ATCCCTGGAA CCCACAGGAA ACGTTTCCAA TATATAACCC AGGTCCTACT ATAGCACCAC 
     3661 CCGTGGACCC CAGAAGTTAT TATGTTAATA ATGCCATAGG ACAAGAAGAA AGCACTCTCT 
     3721 TTCCTTCATG GACCTCCTGG GACCACAGGA ATCAAGCTGA GAGGCAGAAA GAGAGTGAGC 
     3781 CATATTTTAA CAGAAATGTC TGGGATCAGT CAATAAATTT ACACAAATCT AATATACCAA 
     3841 ACCATCCTTA TTCCACTACA TCCCCTGCTA GATTTCCAAA AGATCCAACA TGGTTTGAAG 
     3901 GTGAGAATTT GAACTATGAT TTGCAAATTA CTAGTTTAAG TCCACCAGAA AGAGAACAGT 
     3961 TGGCTTTCCC AGACTTCCTG CCTCAAAGTT ACCCAACAGG TCAAAATGAA GCACACTTAT 
     4021 TTCACCAAAG TCAAAGAGGG TCTTGCTGTA TTGGTGGCTC CACAGGACAT AAAGACAATG 
     4081 TGCTGGCTCT ACAAGACTAC ACTTCATCCT ATGGTCTTCC ACCAAGGAAG AACCAAGAAA 
     4141 CCAGTCCAGT GCATACAGAA AGCAGTTATA TCAAGTATGC AAGACCTAAC GTTTCCCCAG 
     4201 CAAGCATCCT ACCTAGTCAA AGAAATATCT CAGAGAACAA ACTAACTGCA GAAAGCCCAA 
     4261 ACCCAAGTCC ATTTGGAGAT GGTGTGCCTA CTGTGAGGAA AAATACTCCA TATTCTGGAA 
     4321 AGAATCAACT AGAGACAGGA ATTGTGGCCT TTTCTGAAGC CAGCTCTTCT CAGCCAAAAA 
     4381 ACACGCCCTG TCTTAAAAGT GACCTTGGAG GAGATCGGAG GGATGTTCTG AAACAATTTT 
     4441 TTGAAGGCAG CCAGCTGAGT GAAAGAACTG CTGGCCTTAC TCCTGAGCAG CTCGTCATTG 
     4501 GTATTCCTGA TAAAGGCTCT GGCCCAGATA GCATACAAAG TGAAGTCCAA GGAAAAGAGG 
     4561 GTGAGATGCA GCAACAAAGG CCACCTACCA TCATGAAGTT GCCATGCTTT GGCTCCAATT 
     4621 CAAAATTTCA CTCTTCTACC ACTGGACCTC CAATTAACAA CAGAAGACCA ACCCTACTTA 
     4681 ATGGTGCTCT CTCCACACCC ACTGAAAGTC CTAACACATT GGTTGGGTTA GCTACTAGGG 
     4741 AACAACTTAA AAGTATAAAC GTAGATAAAC TTAATGCAGA TGAACACACT ACACTCGAAT 
     4801 CTTTTCAAGG AACCAGTCCA CAGGACCAAG GCTGCTTACT GCTTCAGGCT ACGCGTACGC 
     4861 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     4921 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     4981 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     5041 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     5101 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     5161 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     5221 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     5281 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     5341 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     5401 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     5461 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     5521 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     5581 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     5641 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     5701 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     5761 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     5821 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     5881 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     5941 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     6001 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     6061 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     6121 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     6181 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     6241 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     6301 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     6361 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     6421 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     6481 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     6541 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     6601 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     6661 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     6721 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     6781 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     6841 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     6901 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     6961 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     7021 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     7081 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     7141 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     7201 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     7261 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     7321 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     7381 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     7441 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     7501 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     7561 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     7621 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     7681 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     7741 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     7801 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     7861 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     7921 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     7981 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     8041 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     8101 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     8161 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     8221 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     8281 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     8341 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     8401 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     8461 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     8521 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     8581 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     8641 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     8701 TTT
//