LOCUS       MR226518                9129 bp    DNA     circular  SYN 
DEFINITION  Blm (Myc-DDK-tagged) - Mouse Bloom syndrome, RecQ helicase-like (Blm), transcript variant 2
ACCESSION   NM_001042527
VERSION     NM_001042527.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5286..5377 
                     /label="Myc-DDK"  
     misc_feature    5407..5992 
                     /label="PolyA signal"  
     misc_feature    5464..5483 
                     /label="Restriction Enzyme"  
     misc_feature    6268..6940 
                     /label="ColE1"  
     misc_feature    7370..8164 
                     /label="Kan/Neo"  
     misc_feature    8199..8501 
                     /label="SV40 ori"  
     misc_feature    8587..9064 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5268..5274 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5268 
                     /label="Blm"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTGCTGTT CCTCTGAACA ATCTACAAGA ACAACTACAG CGACACTCAG 
     1081 CCAGAAAACT TAATAATCAA CCCAGCCTTT CAAAACCAAA ATCTTTAGGT TTTACTTTTA 
     1141 AAAAGAAAAC ATCAGAGGGT GATGTGTCTG TCACTAGTGT GTCCGTAGTA AAAACACCTG 
     1201 CGTTAAGTGA TAAAGATGTG AACGTGTCTG AGGCCTTTTC ATTCACTGAG TCTCCACTCC 
     1261 ACAAACCAAA GCAGCAGGCA AAGATTGAAG GCTTCTTTAA ACATTTCCCT GGAAGGCAGC 
     1321 AAAGCAAGGG GACCTGCTCT GAGCCGTCAC TGCCGGCCAC GGTACAGACT GCTCAGGACA 
     1381 CTTTGTGCAC TACCCCCAAA ACCCCCACTG CGAAGAAACT GCCCGTGGCT GTTTTCAAGA 
     1441 AATTAGAATT TAGTTCTTCT GCAGACTCCC TCAGTGACTG GGCTGATATG GATGACTTTG 
     1501 ATATGTCAGC ATCAGATGCG TTTGCTTCAC TGGCTAAAAA TCCTGCCACA AGAGTAAGCA 
     1561 CCGCTCAGAA AATGAAAAAG ACTAAGAGAA ACTTCTTTAA ACCACCACCT CGTAAAGCCA 
     1621 ATGCAGTAAA GACTGACTTG ACTCCTCCCT CCCCCGAATG CCTGCAAGTG GATTTAACGA 
     1681 AGGAATCGGA GGAGGAGGAG GAGGAGGAGG AGGAGGCGGA GGGGGCGGAC TGCCTGAGCA 
     1741 GGGATGTGAT CTGCATTGAC AATGATTCTG CTTCTGAAGA GCTCACGGAG AAAGACACGC 
     1801 AGGAAAGCCA GTCTTTGAAA GCTCACTTGG GAGCTGAAAG AGGTGACAGT GAAAAGAAGA 
     1861 GCCATGAAGA CGAAGCTGTG TTCCATTCAG TTCAGAACAC TGAATACTTT GAACACAATG 
     1921 ACAATGATTA TGATATAGAT TTTGTTCCAC CTTCTCCAGA AGAAATCATC TCCACTGCTT 
     1981 CTTCCTCGTT GAAATGTTCC AGTATGTTAA AGGATCTTGA TGACTCTGAC AAAGAAAAGG 
     2041 GCATTCTTAG CACCTCAGAA GAGCTTCTGT CAAAACCAGA GGAAATGACC ACACACAAGT 
     2101 CTGATGCAGG AACCAGTAAA GACTGTGATG CCCAGCAGAT ACGCATACAG CAGCAGCTTA 
     2161 TTCATGTGAT GGAGCACATC TGTAAGTTAG TTGATACTGT TCCTACTGAT GAACTGGAAG 
     2221 CTTTGAATTG TGGGACCGAA TTGCTTCAAC AACGAAACAT AAGGAGGAAG CTCCTAGCTG 
     2281 AAGCAGGTTT TAATGGAAAT GACGTCAGAC TTCTGGGTTC TCTGTGGAGG CACAGGCCTG 
     2341 ATTCACTTGA TAACACAGTG CAGGGCGACT CCTGCCCTGT GGGGCATCCT AATAAAGAGT 
     2401 TAAATTCTCC ATACCTTCTC TCACATTCCC CTTCCACTGA GGAATGTTTA CCCACCACCA 
     2461 CTCCAGGAAA GACAGGATTC TCAGCCACCC CGAAGAATCT CTTTGAAAGG CCGTTATTGA 
     2521 ATTCCCATTT ACAGAAGTCC TTTGTAAGTA GCAACTGGGC TGAAACACCA AGGATGGAAA 
     2581 ACAGGAACGA AAGCACTGAC TTCCCAGGGA GTGTTCTCAC CAGCACCACT GTGAAAGCTC 
     2641 AGAGTAAACA AGCTGCTTCA GGATGGAACG TAGAGAGACA CGGCCAGGCT TCCTATGATA 
     2701 TCGATAACTT TAATATTGAT GACTTTGATG ATGATGATGA TGATGATGAC TGGGAAAACA 
     2761 TAATGCACAA TTTTCCAGCC AGCAAATCTT CCACAGCCAC CTACCCACCC ATCAAGGAAG 
     2821 GTGGGCCAGT TAAATCTCTC TCAGAAAGGA TTTCTTCAGC CAAGGCAAAG TTTCTTCCAG 
     2881 TGGTATCAAC CGCTCAAAAT ACAAACCTCT CAGAGTCAAT TCAGAATTGC TCTGATAAGC 
     2941 TGGCCCAAAA TTTATCATCA AAAAATCCAA AACATGAACA TTTTCAAAGT CTTAATTTTC 
     3001 CTCATACAAA AGAAATGATG AAGATTTTCC ATAAGAAATT TGGCTTGCAT AATTTTAGAA 
     3061 CTAATCAGCT AGAGGCGATC AATGCTGCGC TGCTTGGTGA AGACTGCTTT ATCCTAATGC 
     3121 CCACTGGAGG AGGTAAAAGT TTGTGCTACC AGCTCCCTGC CTGTGTTTCT CCTGGGGTCA 
     3181 CAATTGTCAT TTCTCCCTTG AGATCACTAA TAGTAGATCA AGTCCAAAAG CTGACTTCCT 
     3241 TTGATATTCC AGCTACATAT CTGACAGGGG ATAAGACTGA CTCAGAAGCT GCAAATATTT 
     3301 ACCTCCAATT ATCCAAAAAA GACCCAATCA TCAAGCTTTT GTATGTTACT CCAGAGAAGG 
     3361 TCTGTGCAAG TAACAGGCTG ATTTCTACTC TGGAGAATCT GTATGAGCGG AAGCTCTTGG 
     3421 CACGTTTTGT CATTGATGAA GCGCATTGTG TGAGTCAGTG GGGTCATGAT TTTCGTCAAG 
     3481 ATTACAAAAG GATGAATATG CTTCGCCAGA AGTTTCCTTC TGTTCCAGTG ATGGCCCTCA 
     3541 CGGCCACAGC GAACCCCAGG GTCCAGAAGG ACATCCTCAC TCAGCTGAAG ATCCTCAGAC 
     3601 CTCAGGTGTT TAGCATGAGC TTTAACAGAC ACAATCTGAA GTACTATGTA TTACCCAAGA 
     3661 AGCCCAAAAA AGTAGCATTT GATTGCCTAG AGTGGATCAG AAAGCATCAC CCTTATGACT 
     3721 CGGGGATAAT TTACTGCCTC TCCAGGAGGG AATGTGACAC AATGGCTGAC ACTTTACAGA 
     3781 GAGAAGGCCT GGCTGCCCTG GCTTACCATG CGGGCCTCAG TGACTCTGCC AGAGATGAGG 
     3841 TGCAGCACAA GTGGATCAAC CAGGACAACT GCCAGGTTAT CTGTGCGACA ATTGCGTTTG 
     3901 GAATGGGAAT TGACAAACCT GACGTGCGAT TTGTGATTCA TGCATCTCTT CCTAAATCTA 
     3961 TGGAGGGTTA TTATCAAGAA TCCGGCCGAG CTGGAAGAGA TGGGGAAATA TCTCACTGCG 
     4021 TGCTTTTCTA TACATATCAT GATGTGACCA GACTGAAGAG ACTTATAATG ATGGAAAAAG 
     4081 ATGGAAACTA TCATACGAAG GAAACTCACG TCAATAATCT ATATAGCATG GTACATTACT 
     4141 GTGAAAACAT AACGGAATGC AGAAGAATAC AGCTTTTAGC TTACTTTGGT GAAAAAGGAT 
     4201 TCAACCCTGA TTTTTGTAAG AAATACCCAG ATGTTTCTTG TGACAATTGC TGTAAAACAA 
     4261 AGGATTATAA AACAAAAGAT GTGACTGATG ACGTGAAAAA TATTATAAGA TTTGTTCAAG 
     4321 AACACAGTTC ATCACCAGGA ACAAGAAATA TAGGACCTGC TGGAAGATTT ACTCTGAACA 
     4381 TGCTGGTCGA CATTTTCTTG GGGAGCAAGA GTGCAAAAGT TAAGTCTGGA ATATTTGGAA 
     4441 AGGGGACTAC ATATTCACGA CATAATGCCG AAAGACTTTT TAAAAAGCTG ATTCTAGACA 
     4501 AAATCCTGGA TGAAGACTTA TATATCAATG CCAATGACCA ACCAATTGCC TATGTGATGC 
     4561 TAGGAACAAA AGCCCACAGT GTGCTGAGTG GCCACTTGAA GGTGGACTTC ATGGAAACGG 
     4621 AAAATTCCAG CAGTATTAAA AAACAAAAAG CTTTAGTGGC CAAAGTATCC CAGAGAGAAG 
     4681 AGGTAGTTAA GAAATGTCTT GGAGAACTTA CAGAGGTCTG CAAATTGCTG GGGAAAGTCT 
     4741 TTGGTGTCCA TTACTTCAAT ATTTTTAATA CAGCCACACT CAAAAAGCTT GCAGAATCTT 
     4801 TATCTTCTGA TCCTGAGGTT TTGCTTCAGA TTGATGGTGT TACCGAAGAC AAGCTGGAAA 
     4861 AATATGGTGC AGAAGTGATT CCAGTATTAC AGAAGTACTC AGAATGGACA GTGCCAGCTG 
     4921 AGGATGGTTC CCCAGGCGCC AGAGGCGCCC CAGAGGACAC TGAGGAGGAG GAGGAGGAAG 
     4981 CGCCTGTATC TTCTCACTAC TTTGCAAATC AAACTAGAAA TGAAAGAAAG AGAAAGAAAA 
     5041 TGTCAGCCAC CCATAAGCCC AAGAGGAGAA GAACTAGTTA CGGTGGCTTC AGAGCAAAGG 
     5101 GGGGCTCTAC TACATGCAGA AAAACGACTT CTAAAAGTAA ATTCTATGGT GTAACTGGAT 
     5161 CCCGCTCGGC CTCATGTGCT TCTCAGGCAA CATCATCAGC CAGTAGAAAA CTGGGGATTA 
     5221 TGGCTCCTCC AAAGCCTGTA AATAGAACGT TCCTCAGGCC TTCATATGCC TTCTCCACGC 
     5281 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     5341 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     5401 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     5461 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     5521 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     5581 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     5641 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     5701 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     5761 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     5821 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     5881 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     5941 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     6001 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     6061 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     6121 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     6181 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     6241 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     6301 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     6361 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     6421 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     6481 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     6541 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     6601 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     6661 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     6721 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     6781 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     6841 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     6901 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     6961 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     7021 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     7081 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     7141 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     7201 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     7261 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     7321 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     7381 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     7441 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     7501 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     7561 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     7621 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     7681 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     7741 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     7801 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     7861 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     7921 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     7981 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     8041 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     8101 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     8161 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     8221 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     8281 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     8341 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     8401 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     8461 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     8521 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     8581 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     8641 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     8701 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     8761 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     8821 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     8881 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     8941 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     9001 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     9061 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     9121 GCGAATTTT
//