LOCUS       MR226506                7974 bp    DNA     circular  SYN 
DEFINITION  Ddx46 (Myc-DDK-tagged) - Mouse DEAD (Asp-Glu-Ala-Asp) box polypeptide 46 (Ddx46)
ACCESSION   NM_145975
VERSION     NM_145975.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4131..4222 
                     /label="Myc-DDK"  
     misc_feature    4252..4837 
                     /label="PolyA signal"  
     misc_feature    4309..4328 
                     /label="Restriction Enzyme"  
     misc_feature    5113..5785 
                     /label="ColE1"  
     misc_feature    6215..7009 
                     /label="Kan/Neo"  
     misc_feature    7044..7346 
                     /label="SV40 ori"  
     misc_feature    7432..7909 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4113..4119 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4113 
                     /label="Ddx46"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGCCGGGAG TCTCGCCACT ACCGAAAACG CTCAGCATCT CGAGGTCGTT 
     1081 CTGGAAGTCG ATCGAGGAGT CGCTCACCCT CTGACAAAAG AAGCAAACGT GGGGATGACA 
     1141 GACGCTCTCG GAGCAGAGAC AGAGACCGGA GGAGAGAGAG GTCTCGGAGT AGGGACAAGA 
     1201 GAAGATCTCG GTCAAGGGAC AGGAAGCGCC TGAGGCGCTC CAGAAGTAGA GAGCGAGACA 
     1261 GAAGCCGAGA GCGGAGAAGA TCTCGGAGCA GAGATAGAAG GCGCTCAAGG AGTCGAAGCC 
     1321 GGGGCAGGCG GTCCCGGTCC TCCAGTCCTG GCAGCAAAAC CAAGAAGACT GAGAACAGGT 
     1381 CTAGGTCCAA AGAGAAAGCA GAAGGTGGGG ACAGCTCCAA GGAGAAGAAA AAGGACAAGG 
     1441 ATGACAAGGA GGACGAGAAA GAGAAGGATG CTGGGAACTT TGACCAGAAT AAACTGGAAG 
     1501 AAGAAATGAG AAAACGGAAA GAAAGAGTTG AAAAATGGCG CGAAGAGCAG CGTAAGAAGG 
     1561 CTATGGAGAA CATAGGAGAG CTGAAGAAGG AGATTGAGGA GATGAAGCAG GGGAAGAAGT 
     1621 GGAGCTTGGA GGACGACGAC GATGATGAAG ATGATCCTGC CGAGGCTGAG AAGGAAGGGA 
     1681 CTGAAATGGA GGACGAGGAG CTGGACCCAT TAGACGCCTA CATGGAAGAA GTGAAGGAAG 
     1741 AGGTAAAAAA GTTTAACATG AGGAGCGTGA AAGGCGGCGC AGGAAATGAG AAGAAGTCTG 
     1801 GGCCAACAGT CACAAAAGTT GTCACTGTGG TGACCACCAA GAAAGCAGTA GTAGACGCGG 
     1861 ATAAGAAGAA GGGGGAACTC ATGGAGAATG ACCAGGACGC CATGGAGTAT TCCTCAGAGG 
     1921 AGGAGGAAGT TGACCTTCAA ACGGCCCTCA CAGGCTACCA GACAAAACAG AGAAAACTTC 
     1981 TAGAGCCAGT TGATCATGGA AAAATTGAAT ATGAGCCATT CAGAAAAAAC TTTTATGTTG 
     2041 AAGTTCCAGA ATTAGCAAAA ATGTCTCAAG AAGAGGTGAA TGTGTTTCGA TTGGAAATGG 
     2101 AGGGCATTAC AGTTAAAGGA AAAGGTTGCC CGAAACCAAT TAAATCATGG GTCCAGTGTG 
     2161 GAATTTCCAT GAAGATACTA AATTCCCTCA AAAAGCATGG CTATGAAAAG CCAACACCAA 
     2221 TCCAAACTCA AGCGATTCCT GCTATAATGT CTGGACGAGA TTTGATTGGC ATTGCCAAGA 
     2281 CAGGAAGTGG AAAAACCATT GCTTTTCTTT TGCCCATGTT TAGACACATC ATGGATCAGA 
     2341 GGTCATTAGA AGAAGGAGAG GGACCAATAG CTGTCATCAT GACTCCAACT CGAGAGCTGG 
     2401 CTTTACAAAT CACTAAAGAA TGTAAGAAGT TTTCGAAGAC CTTGGGACTG AGAGTGGTCT 
     2461 GTGTGTATGG AGGGACAGGA ATCAGCGAGC AGATTGCTGA GCTGAAAAGA GGTGCTGAAA 
     2521 TTATTGTTTG CACACCTGGT CGAATGATTG ACATGTTAGC GGCTAACAGT GGTCGGGTCA 
     2581 CAAATCTTCG AAGAGTGACA TATGTTGTTT TAGATGAAGC AGACAGAATG TTTGACATGG 
     2641 GTTTTGAACC ACAGGTCATG CGCATTGTGG ATAACGTTCG TCCTGACCGA CAGACAGTTA 
     2701 TGTTTTCAGC CACTTTCCCT AGAGCTATGG AGGCTTTGGC CCGCAGGATC CTGAGCAAAC 
     2761 CTATTGAAGT GCAGGTTGGA GGCAGGAGTG TGGTTTGCTC CGATGTGGAA CAGCAGGTGA 
     2821 TTGTGATTGA AGAAGAAAAG AAATTCTTGA AATTGCTGGA GCTTTTAGGC CATTATCAGG 
     2881 AGTCAGGATC TGTCATTATA TTTGTGGATA AGCAGGAACA CGCAGATGGT CTTCTGAAGG 
     2941 ACTTGATGAG AGCATCGTAC CCTTGCATGT CTCTTCACGG AGGTATTGAT CAATATGACA 
     3001 GGGACAGTAT CATAAATGAC TTTAAGAATG GAACCTGCAA GCTCCTCGTT GCCACATCTG 
     3061 TTGCAGCTCG TGGTCTCGAT GTGAAACATC TAATTCTTGT AGTGAATTAC AGCTGCCCCA 
     3121 ATCATTATGA AGATTATGTG CACAGAGCTG GGCGGACTGG AAGAGCGGGC AACAAGGGTT 
     3181 ATGCCTACAC CTTCATCACA GAGGATCAAG CTCGATATGC TGGTGACATC ATTAAAGCTC 
     3241 TTGAATTGTC AGGGACCGCA GTGCCTCCTG ACCTAGAAAA ACTCTGGAGT GACTTCAAAG 
     3301 ATCAACAGAA AGCTGAAGGA AAAATAATTA AAAAGAGTAG TGGCTTCTCT GGCAAGGGAT 
     3361 TCAAGTTTGA TGAAACAGAA CAGGCTTTAG CCAATGAGAG GAAAAAGCTG CAGAAAGCTG 
     3421 CTCTTGGCCT GCAAGATTCT GATGACGAGG ATGCTGCAGT TGATATTGAT GAACAGATTG 
     3481 AAAGCATGTT TAATTCAAAG AAGAGAGTAA AGGACATGGC TGCACCCGGC ACATCCAGCG 
     3541 TGCCCGCGCC CACTGCAGGC AATGCTGAGA AGCTAGAAAT CGCCAAGAGA TTGGCTCTCA 
     3601 GAATCAACGC TCAGAAGAAC CTGGGCATCG AGTCTCAGGA TGTGATGCAG CAGGCCACCA 
     3661 ACGCCATCCT CAGAGGTGGC ACTATCCTGG CTCCCACTGT GTCTGCGAAA ACCATTGCAG 
     3721 AGCAGCTTGC TGAGAAGATC AATGCCAAGC TCAACTACGT GCCTTTGGAG AAGCAGGAGG 
     3781 AAGAGAGACA GGAGGGTGGG CAGAGCGAGT CTTTCAAGAG ATACGAGGAA GAATTAGAAA 
     3841 TTAATGACTT CCCACAGACT GCGAGGTGGA AAGTCACCTC AAAGGAAGCC CTGCAGAGGA 
     3901 TCAGCGAGTA CTCTGAGGCC GCCATCACCA TCAGGGGCAC CTACTTCCCT CCCGGCAAGG 
     3961 AGCCCAAGGA AGGAGAGCGC AAGATCTACC TGGCCATCGA AAGTGCCAAC GAGCTGGCTG 
     4021 TGCAGAAAGC AAAGGCAGAG ATCACCAGGC TCATAAAAGA GGAACTGATC CGACTGCAAA 
     4081 ATTCATACCA ACCAACCAAC AAAGGACGAT ACAAAGTATT AACGCGTACG CGGCCGCTCG 
     4141 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     4201 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     4261 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     4321 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     4381 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     4441 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     4501 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     4561 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     4621 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     4681 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     4741 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     4801 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     4861 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     4921 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     4981 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     5041 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     5101 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     5161 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     5221 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     5281 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     5341 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     5401 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     5461 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     5521 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     5581 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     5641 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     5701 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     5761 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     5821 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     5881 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     5941 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     6001 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     6061 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     6121 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     6181 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     6241 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     6301 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     6361 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     6421 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     6481 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     6541 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     6601 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     6661 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     6721 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     6781 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     6841 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     6901 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     6961 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     7021 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     7081 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     7141 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     7201 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7261 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     7321 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     7381 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     7441 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     7501 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     7561 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     7621 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     7681 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     7741 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     7801 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     7861 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     7921 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//