LOCUS       MR226483                8604 bp    DNA     circular  SYN 
DEFINITION  Phka1 (Myc-DDK-tagged) - Mouse phosphorylase kinase alpha 1 (Phka1), transcript variant 1
ACCESSION   NM_008832
VERSION     NM_008832.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4761..4852 
                     /label="Myc-DDK"  
     misc_feature    4882..5467 
                     /label="PolyA signal"  
     misc_feature    4939..4958 
                     /label="Restriction Enzyme"  
     misc_feature    5743..6415 
                     /label="ColE1"  
     misc_feature    6845..7639 
                     /label="Kan/Neo"  
     misc_feature    7674..7976 
                     /label="SV40 ori"  
     misc_feature    8062..8539 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4743..4749 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4743 
                     /label="Phka1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGGAGCCGC AGTAACTCGG GAGTCCGCCT GGACGGCTAT GCTCGGCTGG 
     1081 TGCATCAGAC CATCCTGTGC CATCAGAATC CAGTGACAGG CTTGCTTCCA GCCAGCTATG 
     1141 ATCAAAAAGA TGCCTGGGTC CGAGATAATG TGTACAGTAT CCTGGCTGTG TGGGGTTTGG 
     1201 GCCTGGCATA TCGCAAAAAT GCTGACCGTG ATGAGGACAA GGCAAAGGCC TATGAACTGG 
     1261 AGCAGAGTGT AGTGAAGTTA ATGAGGGGAC TGCTGCACTG CATGATCAGA CAGGTGGATA 
     1321 AAGTAGAGTC CTTCAAGTAC AGTCAGAGTA CTAAAGATAG CCTCCATGCC AAGTACAACA 
     1381 CCAAAACTTG TGCCACTGTG GTGGGTGATG ACCAGTGGGG ACACCTGCAG TTGGATGCTA 
     1441 CTTCTGTGTA CCTGCTCTTC CTAGCACAAA TGACTGCCTC AGGACTCCAT ATCATCCACA 
     1501 GCCTAGACGA AGTCAATTTT ATACAGAACC TCGTGTTTTA CATTGAAGCT GCATATAAAA 
     1561 CTGCTGACTT TGGGATATGG GAACGTGGCG ATAAGACAAA CCAAGGCATC TCGGAATTGA 
     1621 ATGCGAGTTC AGTTGGAATG GCCAAGGCAG CCCTGGAAGC ACTAGATGAA TTAGACTTGT 
     1681 TTGGTGTGAA AGGTGGGCCA CAATCAGTTA TCCATGTCCT GGCTGATGAA GTCCAACACT 
     1741 GCCAGTCTAT CCTGAATTCA CTACTGCCCA GGGCTTCAAC ATCCAAAGAA GTTGATGCCA 
     1801 GTCTGCTCTC AGTGGTCTCT TTCCCAGCCT TTGCTGTAGA GGACAGCCAT TTGGTGGAGC 
     1861 TCACCAAACA GGAGATCATC ACCAAGCTTC AGGGTCGTTA TGGTTGCTGT CGTTTTCTGC 
     1921 GAGATGGATA TAAAACTCCT AAAGAGGATC CCAATCGCCT ATACTATGAA CCAGCTGAGC 
     1981 TGAAGCTATT TGAAAACATT GAGTGCGAAT GGCCATTGTT CTGGACATAC TTTATCCTTG 
     2041 ATGGGATCTT CAGTGGCAAC GTAGAACAGG TTCAAGAATA TAGAGAGGCT CTTGATGCAG 
     2101 TCCTCATCAA GGGCAAAAAT GGAGTCCCTC TTCTTCCAGA GCTGTACAGT GTCCCTCCTG 
     2161 ACAGGGTTGA TGAAGAGTAT CAAAATCCCC ACACTGTGGA TCGAGTCCCT ATGGGAAAAT 
     2221 TGCCTCACAT GTGGGGTCAG TCTCTATACA TTTTAGGAAG CTTGATGGCA GAGGGATTTT 
     2281 TAGCTCCTGG AGAAATTGAT CCCCTGAATC GTAGGTTTTC TACTGTGCCA AAGCCAGATG 
     2341 TGGTGGTTCA AGTCTCCATT CTGGCTGAAA CAGAAGAAAT CAAGGCCATT TTGAAGGACA 
     2401 AAGGAATTGA TGTGGAGACC ATTGCTGAAG TGTACCCCAT AAGAGTACAG CCAGCTCGTA 
     2461 TTCTCAGCCA TATTTATTCT AGTCTAGGAT GCAACAGTAG AATGAAACTC AGTGGACGAC 
     2521 CCTACAGGCT CATGGGTGTG CTTGGAACAT CAAAACTTTA TGACATTCGC AAAACTATCT 
     2581 TTACTTTCAC TCCACAGTTT ATAGACCAGC AACAGTTCTA CCTGGCTCTG GACAACCAGA 
     2641 TGATAGTAGA AATGCTCAGA ACAGACCTTT CCTACCTCTG TAGCCGCTGG AGGATGACAG 
     2701 GCCAGCCCAC GATCACTTTC CCTATCTCGC ACACCATGCT TGATGAAGAT GGAACCAGCT 
     2761 TGAATTCAAG TATCTTGGCA GCACTCCGAA AAATGCAGGA TGGCTATTTT GGTGGGGCCA 
     2821 GGATCCAAAC AGGTAAATTG TCAGAGTTTT TGACAACATC TTGCTGCACA CACTTAAGCT 
     2881 TCATGGACCC TGGACCTGAG GGTAAGCTGT ACAGTGAAGA TTATGATGAA GACTATGAAG 
     2941 ATGATTTGGA CTCTGGCAAC TGGATGGACA GCTATGATTC AACAAGTAAT GCTCGCTGTG 
     3001 GTGATGAAGT TGCCCGTTAT TTAGACCGCC TTTTGGCACA CACTGTTCCC CATCCTAAAC 
     3061 TAGCTCCTAC CTCACGGAAG GGAGGGCTAG ATCGGTTCCG AGCTGCTGTG CAAACAACTT 
     3121 GCGACTTAAT GTCCTTGGTG GCCAAGGCCA AGGAGCTGCA TATACAGAAT GTTCACATGT 
     3181 ATCTTCCTAC AAAGTTATTT CAGCCTTCTC GTCCTTCACT CAACTTACTT GACTCCCCTG 
     3241 AGTCTCCACA AGATAGCCAG GTTCCTTCCG TTCATGTAGA AGTGCATCTT CCTAGGGACC 
     3301 AGTCTGGGGA AGTGGACTTC CAGTCATTGG TTTCACAGTT GAAGGAGACC TCAAGCTTAC 
     3361 AGGAGCAAGC TGATATACTC TACATGCTGT ATTCAATGAA AGGACCTGAC TGGAACACTG 
     3421 AATTGTATGA AGAAGGGGGG GCTACTGTCA GAGAGCTTCT TAGTGAACTG TATGTCAAAG 
     3481 TTGGTGAAAT TCGGCACTGG GGTCTGATCC GATATATCTC TGGGATCTTA CGGAAGAAAG 
     3541 TGGAGGCACT TGATGAGGCC TGCACAGACC TTCTGTCCTA CCAGAAACAC CTGACAGTAG 
     3601 GACTTCCTCC AGAACCTCGA GAGAAGACCA TCTCTGCGCC TCTACCGTAC GAGGCACTCA 
     3661 CTAAGCTGAT AGATGAAGCC AGTGAAGGCG ACATGAGCAT CTCAACCCTT ACACAGGAAA 
     3721 TAATGGTCTA TCTTGCCATG TATATGAGAA CTCAGCCTGG CCTCTTTGCA GAAATGTTCA 
     3781 GACTTCGAAT CGGTTTGATC ATACAAGTTA TGGCAACAGA ACTAGCACAC TCTCTTCGAT 
     3841 GTTCAGCTGA GGAAGCCACA GAGGGCCTGA TGAATCTCAG TCCTTCAGCC ATGAAGAACC 
     3901 TCCTGCATCA CATTCTCAGT GGCAAAGAGT TTGGAGTGGA ACGAAGCGTT CGTCCCACTG 
     3961 ATTCAAATGT GAGTCCTGCT ATTTCCATCC ATGAGATTGG TGCTGTTGGA GCAACAAAAA 
     4021 CTGAACGAAC TGGAATCATG CAGTTAAAAA GTGAGATAAA GCAGGTGGAA TTTCGTAGGC 
     4081 TGTCTGTCTC GATGGAGAGT CAGACTAGTG GTGGTCATCC CTCGGGTGTA GATTTGATGT 
     4141 CGCCATCCTT TCTGTCCCCT GCAGCCTGTA TTGCTGCAAG TAGTGGATCC TTTCCTACGG 
     4201 TGTGTGATCA TCAGACATCT AAAGATAGTC GTCAAGGCCA GTGGCAACGC AGGAGAAGGC 
     4261 TAGATGGAGC ACTAAATAGA GTACCAATTG GATTTTATCA AAAAGTATGG AAAATTTTGC 
     4321 AGAAATGTCA TGGGCTTTCT GTGGAAGGTT TTGTTCTTCC CTCTTCAACC ACTAGGGAGA 
     4381 TGACCCCAGG TGAGATTAAA TTCTCTGTCC ATGTGGAGTC TGTCCTGAAT CGTGTCCCTC 
     4441 AGCCAGAATA CCGCCAACTT CTGGTTGAAG CCATCCTTGT TCTCACCATG CTGGCAGATA 
     4501 TTGAAATTCA TAGCATTGGG AGCATCATTG CTGTGGAGAA AATAGTTCAT ATTGCCAACG 
     4561 ACTTGTTCCT TCAAGAACAG AAAACCCTCG GCGCAGATGA TACCATGTTG GCAAAGGATC 
     4621 CTGCATCTGG CATCTGTACT CTCCTGTATG ACAGTGCACC CAGTGGCAGA TTTGGCACCA 
     4681 TGACCTACCT CTCCAAGGCA GCTGCCACCT ACGTGCAGGA GTTTCTGCCA CACAGCCTCT 
     4741 GTGCCATGCA GACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     4801 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     4861 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     4921 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     4981 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     5041 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     5101 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     5161 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     5221 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     5281 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     5341 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     5401 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     5461 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     5521 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     5581 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     5641 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     5701 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     5761 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     5821 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     5881 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     5941 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     6001 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     6061 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     6121 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     6181 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     6241 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     6301 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     6361 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     6421 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     6481 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     6541 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     6601 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     6661 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     6721 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     6781 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     6841 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     6901 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     6961 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     7021 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     7081 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     7141 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     7201 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     7261 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     7321 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     7381 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     7441 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     7501 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     7561 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     7621 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     7681 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     7741 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     7801 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     7861 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7921 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7981 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     8041 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     8101 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     8161 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     8221 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     8281 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     8341 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     8401 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     8461 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     8521 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     8581 TAACAAAAAT TTAACGCGAA TTTT
//