LOCUS       MR226481                8427 bp    DNA     circular  SYN 
DEFINITION  Phka1 (Myc-DDK-tagged) - Mouse phosphorylase kinase alpha 1 (Phka1), transcript variant 2
ACCESSION   NM_173021
VERSION     NM_173021.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4584..4675 
                     /label="Myc-DDK"  
     misc_feature    4705..5290 
                     /label="PolyA signal"  
     misc_feature    4762..4781 
                     /label="Restriction Enzyme"  
     misc_feature    5566..6238 
                     /label="ColE1"  
     misc_feature    6668..7462 
                     /label="Kan/Neo"  
     misc_feature    7497..7799 
                     /label="SV40 ori"  
     misc_feature    7885..8362 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4566..4572 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4566 
                     /label="Phka1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGGAGCCGC AGTAACTCGG GAGTCCGCCT GGACGGCTAT GCTCGGCTGG 
     1081 TGCATCAGAC CATCCTGTGC CATCAGAATC CAGTGACAGG CTTGCTTCCA GCCAGCTATG 
     1141 ATCAAAAAGA TGCCTGGGTC CGAGATAATG TGTACAGTAT CCTGGCTGTG TGGGGTTTGG 
     1201 GCCTGGCATA TCGCAAAAAT GCTGACCGTG ATGAGGACAA GGCAAAGGCC TATGAACTGG 
     1261 AGCAGAGTGT AGTGAAGTTA ATGAGGGGAC TGCTGCACTG CATGATCAGA CAGGTGGATA 
     1321 AAGTAGAGTC CTTCAAGTAC AGTCAGAGTA CTAAAGATAG CCTCCATGCC AAGTACAACA 
     1381 CCAAAACTTG TGCCACTGTG GTGGGTGATG ACCAGTGGGG ACACCTGCAG TTGGATGCTA 
     1441 CTTCTGTGTA CCTGCTCTTC CTAGCACAAA TGACTGCCTC AGGACTCCAT ATCATCCACA 
     1501 GCCTAGACGA AGTCAATTTT ATACAGAACC TCGTGTTTTA CATTGAAGCT GCATATAAAA 
     1561 CTGCTGACTT TGGGATATGG GAACGTGGCG ATAAGACAAA CCAAGGCATC TCGGAATTGA 
     1621 ATGCGAGTTC AGTTGGAATG GCCAAGGCAG CCCTGGAAGC ACTAGATGAA TTAGACTTGT 
     1681 TTGGTGTGAA AGGTGGGCCA CAATCAGTTA TCCATGTCCT GGCTGATGAA GTCCAACACT 
     1741 GCCAGTCTAT CCTGAATTCA CTACTGCCCA GGGCTTCAAC ATCCAAAGAA GTTGATGCCA 
     1801 GTCTGCTCTC AGTGGTCTCT TTCCCAGCCT TTGCTGTAGA GGACAGCCAT TTGGTGGAGC 
     1861 TCACCAAACA GGAGATCATC ACCAAGCTTC AGGGTCGTTA TGGTTGCTGT CGTTTTCTGC 
     1921 GAGATGGATA TAAAACTCCT AAAGAGGATC CCAATCGCCT ATACTATGAA CCAGCTGAGC 
     1981 TGAAGCTATT TGAAAACATT GAGTGCGAAT GGCCATTGTT CTGGACATAC TTTATCCTTG 
     2041 ATGGGATCTT CAGTGGCAAC GTAGAACAGG TTCAAGAATA TAGAGAGGCT CTTGATGCAG 
     2101 TCCTCATCAA GGGCAAAAAT GGAGTCCCTC TTCTTCCAGA GCTGTACAGT GTCCCTCCTG 
     2161 ACAGGGTTGA TGAAGAGTAT CAAAATCCCC ACACTGTGGA TCGAGTCCCT ATGGGAAAAT 
     2221 TGCCTCACAT GTGGGGTCAG TCTCTATACA TTTTAGGAAG CTTGATGGCA GAGGGATTTT 
     2281 TAGCTCCTGG AGAAATTGAT CCCCTGAATC GTAGGTTTTC TACTGTGCCA AAGCCAGATG 
     2341 TGGTGGTTCA AGTCTCCATT CTGGCTGAAA CAGAAGAAAT CAAGGCCATT TTGAAGGACA 
     2401 AAGGAATTGA TGTGGAGACC ATTGCTGAAG TGTACCCCAT AAGAGTACAG CCAGCTCGTA 
     2461 TTCTCAGCCA TATTTATTCT AGTCTAGGAT GCAACAGTAG AATGAAACTC AGTGGACGAC 
     2521 CCTACAGGCT CATGGGTGTG CTTGGAACAT CAAAACTTTA TGACATTCGC AAAACTATCT 
     2581 TTACTTTCAC TCCACAGTTT ATAGACCAGC AACAGTTCTA CCTGGCTCTG GACAACCAGA 
     2641 TGATAGTAGA AATGCTCAGA ACAGACCTTT CCTACCTCTG TAGCCGCTGG AGGATGACAG 
     2701 GCCAGCCCAC GATCACTTTC CCTATCTCGC ACACCATGCT TGATGAAGAT GGAACCAGCT 
     2761 TGAATTCAAG TATCTTGGCA GCACTCCGAA AAATGCAGGA TGGCTATTTT GGTGGGGCCA 
     2821 GGATCCAAAC AGGTAAATTG TCAGAGTTTT TGACAACATC TTGCTGCACA CACTTAAGCT 
     2881 TCATGGACCC TGGACCTGAG GGTAAGCTGT ACAGTGAAGA TTATGATGAA GACTATGAAG 
     2941 ATGATTTGGA CTCTGGCAAC TGGATGGACA GCTATGATTC AACAAGTAAT GATGTTCACA 
     3001 TGTATCTTCC TACAAAGTTA TTTCAGCCTT CTCGTCCTTC ACTCAACTTA CTTGACTCCC 
     3061 CTGAGTCTCC ACAAGATAGC CAGGTTCCTT CCGTTCATGT AGAAGTGCAT CTTCCTAGGG 
     3121 ACCAGTCTGG GGAAGTGGAC TTCCAGTCAT TGGTTTCACA GTTGAAGGAG ACCTCAAGCT 
     3181 TACAGGAGCA AGCTGATATA CTCTACATGC TGTATTCAAT GAAAGGACCT GACTGGAACA 
     3241 CTGAATTGTA TGAAGAAGGG GGGGCTACTG TCAGAGAGCT TCTTAGTGAA CTGTATGTCA 
     3301 AAGTTGGTGA AATTCGGCAC TGGGGTCTGA TCCGATATAT CTCTGGGATC TTACGGAAGA 
     3361 AAGTGGAGGC ACTTGATGAG GCCTGCACAG ACCTTCTGTC CTACCAGAAA CACCTGACAG 
     3421 TAGGACTTCC TCCAGAACCT CGAGAGAAGA CCATCTCTGC GCCTCTACCG TACGAGGCAC 
     3481 TCACTAAGCT GATAGATGAA GCCAGTGAAG GCGACATGAG CATCTCAACC CTTACACAGG 
     3541 AAATAATGGT CTATCTTGCC ATGTATATGA GAACTCAGCC TGGCCTCTTT GCAGAAATGT 
     3601 TCAGACTTCG AATCGGTTTG ATCATACAAG TTATGGCAAC AGAACTAGCA CACTCTCTTC 
     3661 GATGTTCAGC TGAGGAAGCC ACAGAGGGCC TGATGAATCT CAGTCCTTCA GCCATGAAGA 
     3721 ACCTCCTGCA TCACATTCTC AGTGGCAAAG AGTTTGGAGT GGAACGAAGC GTTCGTCCCA 
     3781 CTGATTCAAA TGTGAGTCCT GCTATTTCCA TCCATGAGAT TGGTGCTGTT GGAGCAACAA 
     3841 AAACTGAACG AACTGGAATC ATGCAGTTAA AAAGTGAGAT AAAGCAGGTG GAATTTCGTA 
     3901 GGCTGTCTGT CTCGATGGAG AGTCAGACTA GTGGTGGTCA TCCCTCGGGT GTAGATTTGA 
     3961 TGTCGCCATC CTTTCTGTCC CCTGCAGCCT GTATTGCTGC AAGTAGTGGA TCCTTTCCTA 
     4021 CGGTGTGTGA TCATCAGACA TCTAAAGATA GTCGTCAAGG CCAGTGGCAA CGCAGGAGAA 
     4081 GGCTAGATGG AGCACTAAAT AGAGTACCAA TTGGATTTTA TCAAAAAGTA TGGAAAATTT 
     4141 TGCAGAAATG TCATGGGCTT TCTGTGGAAG GTTTTGTTCT TCCCTCTTCA ACCACTAGGG 
     4201 AGATGACCCC AGGTGAGATT AAATTCTCTG TCCATGTGGA GTCTGTCCTG AATCGTGTCC 
     4261 CTCAGCCAGA ATACCGCCAA CTTCTGGTTG AAGCCATCCT TGTTCTCACC ATGCTGGCAG 
     4321 ATATTGAAAT TCATAGCATT GGGAGCATCA TTGCTGTGGA GAAAATAGTT CATATTGCCA 
     4381 ACGACTTGTT CCTTCAAGAA CAGAAAACCC TCGGCGCAGA TGATACCATG TTGGCAAAGG 
     4441 ATCCTGCATC TGGCATCTGT ACTCTCCTGT ATGACAGTGC ACCCAGTGGC AGATTTGGCA 
     4501 CCATGACCTA CCTCTCCAAG GCAGCTGCCA CCTACGTGCA GGAGTTTCTG CCACACAGCC 
     4561 TCTGTGCCAT GCAGACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     4621 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     4681 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     4741 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     4801 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     4861 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     4921 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     4981 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     5041 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     5101 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     5161 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     5221 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     5281 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     5341 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     5401 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     5461 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     5521 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     5581 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     5641 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     5701 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     5761 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     5821 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     5881 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     5941 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     6001 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     6061 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     6121 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     6181 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     6241 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     6301 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     6361 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     6421 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     6481 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     6541 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     6601 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     6661 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     6721 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     6781 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     6841 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     6901 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     6961 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     7021 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     7081 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     7141 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     7201 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     7261 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     7321 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     7381 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     7441 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     7501 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     7561 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     7621 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     7681 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7741 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7801 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     7861 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     7921 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     7981 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     8041 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     8101 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     8161 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     8221 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     8281 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     8341 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     8401 ATTTAACAAA AATTTAACGC GAATTTT
//