LOCUS       MR226459                8613 bp    DNA     circular  SYN 
DEFINITION  Dhx8 (Myc-DDK-tagged) - Mouse DEAH (Asp-Glu-Ala-His) box polypeptide 8 (Dhx8)
ACCESSION   NM_144831
VERSION     NM_144831.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4770..4861 
                     /label="Myc-DDK"  
     misc_feature    4891..5476 
                     /label="PolyA signal"  
     misc_feature    4948..4967 
                     /label="Restriction Enzyme"  
     misc_feature    5752..6424 
                     /label="ColE1"  
     misc_feature    6854..7648 
                     /label="Kan/Neo"  
     misc_feature    7683..7985 
                     /label="SV40 ori"  
     misc_feature    8071..8548 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4752..4758 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4752 
                     /label="Dhx8"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTGTAGCC GTGGCCGCCG CCGGGGTCCT AATGGGCTCG GAGCCCGGCC 
     1081 CGGCGGAAGA ACTCGCTAAA CTTGAGTACC TGTCTTTGGT GTCGAAGGTT TGCACGGAGC 
     1141 TGGACAATCA CTTGGGCATC AACGACAAGG ATCTGGCTGA ATTTGTCATC AGTCTTGCTG 
     1201 AGAAAAATAC TACCTTTGAT ACTTTTAAGG CTTCTCTGGT CAAAAATGGT GCAGAATTCA 
     1261 CAGATTCTCT TATTAGCAAC CTCCTGCGTC TCATACAGAC CATGCGGCCT CCTGCAAAGC 
     1321 CTTCTACCAG TAAAGATCCG GTTGTTAAAC CCAAAACCGA AAAAGAAAAG CTGAGGGAAC 
     1381 TCTTCCCCGT CCTCTGCCAG CCGGACAACC CTTCAGCTCG GACCATGTTG GATGAGGAAG 
     1441 ATGTGAAAGT CGCTGTGGAC GTCTTGAAAG AGCTGGAAGC TTTAATGCCC AGCGCAGCAG 
     1501 GCCAAGAGAA ACAAAGAGAC CCTGAGCACA GGGACAGGAC AAAGAAGAAG AAGAGGAGTC 
     1561 GGAGCCGAGA CCGAGATCGA GACCGAGACC GAGACCGGGA CCGGGACCGG GACCGGGACA 
     1621 GGGACCGTGA CAAGGACCGA GAACGAGATA GAGATCGAGA ACGAGACAGA GAGCGAGATA 
     1681 GAGAGAGAGA CCATAAGCGG AGACACCGAT CACGCTCTCG GTCACATTCT AGAACCCGGG 
     1741 AGAGGACTAA GGGGAAATCT AGATACAGAT CTAGGAGCAG AAGTCAGAGT CCCTTCAAAG 
     1801 ATCGGAAAGA CCGAGAAAAG TATGGGGAGA GGAACTTGGA CAGATGGCGG GATAAGCATG 
     1861 TGGACCGCCC TCCCCCGGAA GAGCCTGCCA TTGGAGATAT TTACAATGGC AAAGTGACCA 
     1921 GTATCATGCA GTTCGGTTGC TTTGTGCAGC TGGAAGGCCT AAGGAAGCGG TGGGAAGGCC 
     1981 TGGTGCACAT CTCTGAGCTC CGACGGGAAG GTCGAGTGGC CAATGTGGCT GATGTTGTGA 
     2041 GTAAAGGCCA GAGAGTCAAG GTCAAAGTTC TGTCCTTCAC TGGAACCAAG ACCAGCCTGA 
     2101 GCATGAAGGA TGTGGACCAA GAGACAGGAG AAGACCTAAA CCCAAACAGA CGGAGAAATC 
     2161 TGGTTGGCGA GACCAATGAA GAGACGTCAA TGCGGAACCC AGACAGACCC ACTCACCTCT 
     2221 CCCTTGTCAG CGCTCCTGAA GTAGAGGATG ACTCGCTGGA GCGCAAGCGG CTCACCCGAA 
     2281 TCTCTGACCC AGAGAAGTGG GAGATCAAAC AGATGATTGC TGCCAATGTG CTTTCCAAAG 
     2341 AAGAGTTTCC AGACTTCGAC GAAGAGACTG GCATTCTCCC CAAGGTGGAT GATGAAGAAG 
     2401 ATGAGGATCT TGAAATTGAA CTGGTTGAAG AGGAGCCTCC GTTCCTGAGA GGGCATACCA 
     2461 AGCAGAGCAT GGATATGAGC CCCATCAAAA TCGTTAAGAA CCCAGATGGC TCACTCTCCC 
     2521 AAGCAGCCAT GATGCAGAGT GCCCTGGCCA AGGAAAGACG GGAGCTGAAG CAGGCCCAGC 
     2581 GGGAAGCAGA GATGGATTCT ATACCCATGG GGCTCAACAA ACACTGGGTT GATCCCTTGC 
     2641 CCGATGCGGA AGGCAGGCAG ATCGCTGCCA ACATGAGGGG GATTGGGATG ATGCCCAACG 
     2701 ACATTCCTGA GTGGAAGAAG CATGCCTTTG GGGGCAACAA AGCTTCTTAT GGGAAGAAGA 
     2761 CCCAGATGTC AATCCTTGAG CAGAGAGAGA GCCTGCCCAT CTACAAACTG AAGGAGCAGC 
     2821 TGGTGCAGGC TGTGCATGAC AATCAGATCC TAATTGTAAT TGGAGAGACA GGGTCTGGGA 
     2881 AGACAACACA GATCACCCAG TACCTGGCAG AGGCAGGCTA TACTTCCAGG GGCAAGATTG 
     2941 GGTGTACCCA GCCCAGAAGA GTGGCAGCTA TGTCTGTGGC CAAAAGAGTC TCAGAAGAGT 
     3001 TTGGTTGTTG CCTTGGTCAA GAGGTGGGCT ACACCATTCG ATTTGAGGAT TGCACCAGCC 
     3061 CAGAAACAGT CATCAAGTAC ATGACAGATG GCATGCTGCT AAGAGAGTGC CTGATTGACC 
     3121 CCGATCTCAC TCAGTATGCC ATCATTATGT TGGATGAAGC ACACGAGAGG ACCATTCACA 
     3181 CAGATGTGCT CTTTGGATTG TTGAAAAAGA CAGTTCAAAA ACGACAAGAC ATGAAGCTGA 
     3241 TTGTCACCTC AGCCACGCTG GATGCGGTGA AGTTTTCTCA GTACTTCTAT GAAGCACCCA 
     3301 TCTTCACAAT CCCAGGTCGA ACCTATCCAG TGGAAATTCT GTACACAAAG GAGCCGGAAA 
     3361 CAGACTATTT GGACGCTAGC CTGATCACGG TCATGCAGAT CCATCTGACA GAGCCGCCAG 
     3421 GTGACATCTT GGTTTTCTTG ACTGGTCAGG AAGAGATCGA CACTGCTTGT GAGATCCTGT 
     3481 ATGAAAGAAT GAAGTCCCTG GGACCTGATG TCCCAGAGTT GATCATCCTC CCAGTGTACT 
     3541 CTGCCCTTCC GAGTGAGATG CAGACCCGAA TCTTTGATCC AGCTCCCCCT GGTAGCAGAA 
     3601 AGGTGGTGAT TGCCACCAAC ATTGCAGAAA CATCTCTGAC CATCGACGGC ATCTACTATG 
     3661 TGGTTGACCC TGGATTTGTG AAGCAGAAAG TGTACAATTC TAAAACAGGG ATCGATCAGC 
     3721 TTGTGGTGAC ACCTATTTCT CAGGCTCAAG CAAAGCAGAG AGCTGGCCGG GCTGGGAGAA 
     3781 CTGGCCCAGG AAAGTGTTAC AGACTGTACA CGGAGCGTGC CTACCGCGAT GAAATGCTGA 
     3841 CCACCAATGT ACCGGAAATC CAGAGAACCA ACCTGGCCAG CACAGTGCTG TCCCTCAAGG 
     3901 CCATGGGTAT TAATGACCTG CTGTCCTTTG ACTTCATGGA TGCCCCACCG ATGGAAACTC 
     3961 TGATCACGGC CATGGAGCAG CTGTATACAC TGGGGGCCCT GGACGACGAG GGCCTGCTCA 
     4021 CTCGCCTGGG CCGCAGGATG GCAGAGTTCC CTCTGGAGCC AATGCTGTGC AAAATGCTCA 
     4081 TCATGTCTGT GCATCTGGGC TGCAGCGAAG AGATGCTAAC TATCGTGTCC ATGCTGTCTG 
     4141 TGCAGAACGT CTTCTACCGA CCCAAGGATA AACAGGCCCT TGCAGATCAG AAGAAAGCCA 
     4201 AATTCCACCA GACAGAAGGG GACCACCTCA CCCTGCTGGC CGTATACAAC TCCTGGAAGA 
     4261 ACAACAAATT CTCCAACCCG TGGTGCTATG AGAATTTTAT TCAGGCTCGT TCCCTACGCC 
     4321 GTGCCCAGGA CATTCGGAAG CAGATGTTAG GCATAATGGA CAGGCACAAG CTGGACGTGG 
     4381 TCTCCTGTGG CAAATCCACA GTCCGAGTGC AGAAGGCCAT CTGCAGTGGG TTCTTCCGGA 
     4441 ACGCTGCCAA GAAGGACCCA CAGGAAGGCT ACCGGACTCT GATAGACCAG CAGGTGGTGT 
     4501 ATATCCACCC CTCCAGTGCT CTCTTCAACA GACAGCCCGA ATGGGTGGTA TACCACGAAC 
     4561 TTGTGCTGAC CACAAAGGAA TACATGCGTG AGGTCACCAC CATCGACCCC CGGTGGCTTG 
     4621 TGGAGTTTGC TCCGGCTTTC TTCAAAGTTT CTGACCCAAC CAAGCTAAGC AAGCAGAAGA 
     4681 AACAGCAGCG CCTGGAGCCT CTGTACAATC GGTATGAGGA GCCGAACGCC TGGAGAATCT 
     4741 CCCGAGCCTT CCGCCGGCGC ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     4801 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     4861 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     4921 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     4981 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     5041 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     5101 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     5161 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     5221 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     5281 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     5341 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     5401 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     5461 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     5521 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     5581 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     5641 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     5701 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     5761 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     5821 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     5881 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     5941 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     6001 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     6061 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     6121 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     6181 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     6241 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     6301 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     6361 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     6421 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     6481 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     6541 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     6601 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     6661 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     6721 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     6781 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     6841 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     6901 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     6961 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     7021 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     7081 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     7141 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     7201 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     7261 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     7321 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     7381 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     7441 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     7501 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     7561 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     7621 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     7681 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     7741 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     7801 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     7861 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7921 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7981 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     8041 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     8101 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     8161 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     8221 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     8281 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     8341 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     8401 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     8461 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     8521 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     8581 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//